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The map label for this gene is kdgA

Identifier: 16079268

GI number: 16079268

Start: 2323009

End: 2323599

Strand: Reverse

Name: kdgA

Synonym: BSU22100

Alternate gene names: 16079268

Gene position: 2323599-2323009 (Counterclockwise)

Preceding gene: 16079269

Following gene: 16079267

Centisome position: 55.12

GC content: 48.73

Gene sequence:

>591_bases
ATGGAGTCCAAAGTCGTTGAAAACCGTCTGAAAGAAGCAAAGCTGATTGCAGTCATTCGTTCAAAGGATAAGCAGGAGGC
CTGTCAGCAGATTGAGAGTTTATTAGATAAAGGGATTCGTGCAGTTGAAGTGACGTATACGACCCCCGGGGCATCAGATA
TTATCGAATCCTTCCGTAATAGGGAAGATATTTTAATTGGCGCGGGTACGGTCATCAGCGCGCAGCAAGCTGGGGAAGCT
GCTAAGGCTGGCGCGCAGTTTATTGTCAGTCCGGGTTTTTCAGCTGATCTTGCTGAACATCTATCTTTTGTAAAGACACA
TTATATCCCCGGCGTCTTGACTCCGAGCGAAATTATGGAAGCGCTGACATTCGGTTTTACGACATTAAAGCTGTTCCCAA
GCGGTGTGTTTGGCATTCCGTTTATGAAAAATTTAGCGGGTCCTTTCCCGCAGGTGACCTTTATTCCGACAGGCGGGATA
CATCCGTCTGAAGTGCCTGATTGGCTTAGAGCCGGAGCTGGCGCCGTCGGAGTCGGCAGCCAGTTGGGCAGCTGTTCAAA
AGAGGATTTGCAGGCTGTTTTCCAAGTGTAA

Upstream 100 bases:

>100_bases
CAGGCACCGGGGGATATGGACGGTTTGCCGACCAGGGAGAAATTAGCTTCTTTTTTATCTGCTCAAAGAACGGTTCACCA
AAAGAAAGGGGATTATTAAT

Downstream 100 bases:

>100_bases
GTGGAATTGTTTTTTCATAACCGGAGGATGGGAGAATCCACAATATGAAAATCAAGGCGACAATTGAACGGGTACCTGGC
GGAATGATGATTATTCCGCT

Product: 2-keto-3-deoxygluconate-6-phosphate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MESKVVENRLKEAKLIAVIRSKDKQEACQQIESLLDKGIRAVEVTYTTPGASDIIESFRNREDILIGAGTVISAQQAGEA
AKAGAQFIVSPGFSADLAEHLSFVKTHYIPGVLTPSEIMEALTFGFTTLKLFPSGVFGIPFMKNLAGPFPQVTFIPTGGI
HPSEVPDWLRAGAGAVGVGSQLGSCSKEDLQAVFQV

Sequences:

>Translated_196_residues
MESKVVENRLKEAKLIAVIRSKDKQEACQQIESLLDKGIRAVEVTYTTPGASDIIESFRNREDILIGAGTVISAQQAGEA
AKAGAQFIVSPGFSADLAEHLSFVKTHYIPGVLTPSEIMEALTFGFTTLKLFPSGVFGIPFMKNLAGPFPQVTFIPTGGI
HPSEVPDWLRAGAGAVGVGSQLGSCSKEDLQAVFQV
>Mature_196_residues
MESKVVENRLKEAKLIAVIRSKDKQEACQQIESLLDKGIRAVEVTYTTPGASDIIESFRNREDILIGAGTVISAQQAGEA
AKAGAQFIVSPGFSADLAEHLSFVKTHYIPGVLTPSEIMEALTFGFTTLKLFPSGVFGIPFMKNLAGPFPQVTFIPTGGI
HPSEVPDWLRAGAGAVGVGSQLGSCSKEDLQAVFQV

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family

Homologues:

Organism=Escherichia coli, GI1788156, Length=147, Percent_Identity=37.4149659863946, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI48994954, Length=173, Percent_Identity=31.2138728323699, Blast_Score=91, Evalue=7e-20,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ALKH_BACSU (P50846)

Other databases:

- EMBL:   L47838
- EMBL:   AL009126
- PIR:   H69647
- RefSeq:   NP_390092.1
- ProteinModelPortal:   P50846
- SMR:   P50846
- EnsemblBacteria:   EBBACT00000000660
- GeneID:   939066
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU22100
- NMPDR:   fig|224308.1.peg.2215
- GenoList:   BSU22100
- GeneTree:   EBGT00050000001976
- HOGENOM:   HBG670118
- OMA:   YANLQIM
- PhylomeDB:   P50846
- ProtClustDB:   CLSK887480
- BioCyc:   BSUB:BSU22100-MONOMER
- BRENDA:   4.1.2.14
- BRENDA:   4.1.3.16
- GO:   GO:0005737
- InterPro:   IPR000887
- InterPro:   IPR013785
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR01182

Pfam domain/function: PF01081 Aldolase

EC number: =4.1.3.16; =4.1.2.14

Molecular weight: Translated: 20865; Mature: 20865

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1; PS00160 ALDOLASE_KDPG_KHG_2

Important sites: ACT_SITE 43-43 ACT_SITE 47-47 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESKVVENRLKEAKLIAVIRSKDKQEACQQIESLLDKGIRAVEVTYTTPGASDIIESFRN
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHCC
REDILIGAGTVISAQQAGEAAKAGAQFIVSPGFSADLAEHLSFVKTHYIPGVLTPSEIME
CCCEEEECCCEECHHHHCHHHHHCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHH
ALTFGFTTLKLFPSGVFGIPFMKNLAGPFPQVTFIPTGGIHPSEVPDWLRAGAGAVGVGS
HHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCEEEEECCCCCHHCCHHHHHHCCCCCCCCH
QLGSCSKEDLQAVFQV
HHCCCCHHHHHHHHCC
>Mature Secondary Structure
MESKVVENRLKEAKLIAVIRSKDKQEACQQIESLLDKGIRAVEVTYTTPGASDIIESFRN
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHCC
REDILIGAGTVISAQQAGEAAKAGAQFIVSPGFSADLAEHLSFVKTHYIPGVLTPSEIME
CCCEEEECCCEECHHHHCHHHHHCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHH
ALTFGFTTLKLFPSGVFGIPFMKNLAGPFPQVTFIPTGGIHPSEVPDWLRAGAGAVGVGS
HHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCEEEEECCCCCHHCCHHHHHHCCCCCCCCH
QLGSCSKEDLQAVFQV
HHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377