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The map label for this gene is ypwA

Identifier: 16079266

GI number: 16079266

Start: 2320355

End: 2321860

Strand: Reverse

Name: ypwA

Synonym: BSU22080

Alternate gene names: 16079266

Gene position: 2321860-2320355 (Counterclockwise)

Preceding gene: 16079267

Following gene: 16079265

Centisome position: 55.08

GC content: 42.1

Gene sequence:

>1506_bases
ATGGAGATACATACATATGAAAAAGAATTTTTTGATCTTTTAAAAAGAATCTCACATTACAGCGAAGCAGTTGCGTTAAT
GCACTGGGACTCCAGAACAGGCGCGCCGAAAAATGGCTCAGAGGATCGTGCCGAAAGTATCGGCCAGCTTTCAACAGATA
TATTCAATATCCAAACATCAGATCGAATGAAGGAGCTTATCGATGTCCTGTATGAACGATTTGATGACTTGTCGGAAGAT
ACGAAAAAAGCAGTGGAACTGGCGAAAAAAGAGTATGAAGAGAATAAGAAAATCCCTGAGGCTGAATACAAAGAATACGT
GATTCTGTGTTCAAAGGCAGAAACAGCGTGGGAGGAAGCGAAAGGAAAATCTGATTTCAGCTTGTTTTCTCCTTATTTAG
AACAGCTGATTGAATTCAATAAACGTTTTATTACGTATTGGGGATATCAAGAGCATCCTTATGATGCATTGCTTGATTTG
TTTGAGCCCGGCGTCACGGTAAAGGTGCTTGATCAGTTGTTTGCTGAGCTGAAGGAAGCGATTATCCCTCTTGTTAAACA
GGTGACAGCTTCCGGAAATAAACCAGATACAAGCTTTATTACAAAAGCGTTCCCGAAAGAAAAGCAAAAAGAGCTCAGTC
TGTATTTTTTACAGGAGCTTGGCTACGATTTTGATGGCGGAAGACTTGATGAAACCGTTCATCCGTTTGCAACTACGCTG
AATCGGGGAGACGTTCGGGTCACGACGAGATATGATGAAAAAGATTTCCGGACGGCGATCTTTGGGACCATTCATGAGTG
CGGGCACGCGATTTACGAGCAAAATATTGATGAAGCACTCAGTGGCACGAACTTGTCAGACGGCGCATCGATGGGAATTC
ATGAATCGCAATCATTATTTTACGAGAATTTTATCGGCCGCAACAAGCATTTTTGGACTCCGTACTATAAGAAGATTCAA
GAGGCATCACCTGTACAGTTCAAGGACATCTCCCTGGATGATTTTGTCCGGGCAATCAATGAATCAAAACCTTCATTTAT
CAGGGTCGAAGCGGATGAATTGACGTATCCTCTTCATATCATCATCCGTTATGAAATTGAAAAAGCGATTTTCAGCAATG
AAGTGTCTGTGGAAGATCTGCCGTCGCTTTGGAATCAAAAATACCAGGATTATTTAGGGATTACACCGCAGACCGACGCA
GAGGGGATTTTACAGGACGTCCATTGGGCAGGTGGAGATTTCGGCTACTTCCCATCCTATGCGCTCGGCTACATGTATGC
GGCTCAGCTGAAACAAAAAATGCTTGAGGATCTCCCTGAATTTGACGCTCTCTTGGAAAGAGGAGAGTTCCATCCGATAA
AGCAATGGCTGACGGAAAAGGTGCACATTCACGGAAAACGGAAAAAGCCGTTAGACATTATTAAAGATGCAACAGGAGAA
GAGCTGAATGTTCGCTATTTAATTGATTATTTATCAAACAAATATTCAAATCTCTATCTGTTATAA

Upstream 100 bases:

>100_bases
GCCACAAAACATACCGGCAGCCTTTTATACAAACAATTTGACTTTCATACCCCCTTCCATTAGCCTGTAATGGAACGGCT
AGAATAGGGAGGGAATGGAT

Downstream 100 bases:

>100_bases
TCAAAAGCCTGGCGGCGCGGTCGTCAGACTCTTTTATATCGAATCCCCTTGAAATACGAATGATATCTAAAAAAACAAAA
TTAAAGTTCGGGAATTTTTA

Product: metal-dependent carboxypeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MEIHTYEKEFFDLLKRISHYSEAVALMHWDSRTGAPKNGSEDRAESIGQLSTDIFNIQTSDRMKELIDVLYERFDDLSED
TKKAVELAKKEYEENKKIPEAEYKEYVILCSKAETAWEEAKGKSDFSLFSPYLEQLIEFNKRFITYWGYQEHPYDALLDL
FEPGVTVKVLDQLFAELKEAIIPLVKQVTASGNKPDTSFITKAFPKEKQKELSLYFLQELGYDFDGGRLDETVHPFATTL
NRGDVRVTTRYDEKDFRTAIFGTIHECGHAIYEQNIDEALSGTNLSDGASMGIHESQSLFYENFIGRNKHFWTPYYKKIQ
EASPVQFKDISLDDFVRAINESKPSFIRVEADELTYPLHIIIRYEIEKAIFSNEVSVEDLPSLWNQKYQDYLGITPQTDA
EGILQDVHWAGGDFGYFPSYALGYMYAAQLKQKMLEDLPEFDALLERGEFHPIKQWLTEKVHIHGKRKKPLDIIKDATGE
ELNVRYLIDYLSNKYSNLYLL

Sequences:

>Translated_501_residues
MEIHTYEKEFFDLLKRISHYSEAVALMHWDSRTGAPKNGSEDRAESIGQLSTDIFNIQTSDRMKELIDVLYERFDDLSED
TKKAVELAKKEYEENKKIPEAEYKEYVILCSKAETAWEEAKGKSDFSLFSPYLEQLIEFNKRFITYWGYQEHPYDALLDL
FEPGVTVKVLDQLFAELKEAIIPLVKQVTASGNKPDTSFITKAFPKEKQKELSLYFLQELGYDFDGGRLDETVHPFATTL
NRGDVRVTTRYDEKDFRTAIFGTIHECGHAIYEQNIDEALSGTNLSDGASMGIHESQSLFYENFIGRNKHFWTPYYKKIQ
EASPVQFKDISLDDFVRAINESKPSFIRVEADELTYPLHIIIRYEIEKAIFSNEVSVEDLPSLWNQKYQDYLGITPQTDA
EGILQDVHWAGGDFGYFPSYALGYMYAAQLKQKMLEDLPEFDALLERGEFHPIKQWLTEKVHIHGKRKKPLDIIKDATGE
ELNVRYLIDYLSNKYSNLYLL
>Mature_501_residues
MEIHTYEKEFFDLLKRISHYSEAVALMHWDSRTGAPKNGSEDRAESIGQLSTDIFNIQTSDRMKELIDVLYERFDDLSED
TKKAVELAKKEYEENKKIPEAEYKEYVILCSKAETAWEEAKGKSDFSLFSPYLEQLIEFNKRFITYWGYQEHPYDALLDL
FEPGVTVKVLDQLFAELKEAIIPLVKQVTASGNKPDTSFITKAFPKEKQKELSLYFLQELGYDFDGGRLDETVHPFATTL
NRGDVRVTTRYDEKDFRTAIFGTIHECGHAIYEQNIDEALSGTNLSDGASMGIHESQSLFYENFIGRNKHFWTPYYKKIQ
EASPVQFKDISLDDFVRAINESKPSFIRVEADELTYPLHIIIRYEIEKAIFSNEVSVEDLPSLWNQKYQDYLGITPQTDA
EGILQDVHWAGGDFGYFPSYALGYMYAAQLKQKMLEDLPEFDALLERGEFHPIKQWLTEKVHIHGKRKKPLDIIKDATGE
ELNVRYLIDYLSNKYSNLYLL

Specific function: Unknown

COG id: COG2317

COG function: function code E; Zn-dependent carboxypeptidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M32 family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YPWA_BACSU (P50848)

Other databases:

- EMBL:   L47838
- EMBL:   L77246
- EMBL:   AL009126
- PIR:   D69943
- RefSeq:   NP_390090.1
- PDB:   3HQ2
- PDBsum:   3HQ2
- ProteinModelPortal:   P50848
- SMR:   P50848
- MEROPS:   M32.002
- EnsemblBacteria:   EBBACT00000000249
- GeneID:   939061
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU22080
- NMPDR:   fig|224308.1.peg.2213
- GenoList:   BSU22080
- GeneTree:   EBGT00050000001421
- HOGENOM:   HBG310233
- OMA:   DGCMQDI
- ProtClustDB:   CLSK2752431
- BioCyc:   BSUB:BSU22080-MONOMER
- GO:   GO:0006508
- InterPro:   IPR001333
- PIRSF:   PIRSF006615
- PRINTS:   PR00998

Pfam domain/function: PF02074 Peptidase_M32

EC number: NA

Molecular weight: Translated: 58175; Mature: 58175

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: ACT_SITE 266-266

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIHTYEKEFFDLLKRISHYSEAVALMHWDSRTGAPKNGSEDRAESIGQLSTDIFNIQTS
CCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHEECCCH
DRMKELIDVLYERFDDLSEDTKKAVELAKKEYEENKKIPEAEYKEYVILCSKAETAWEEA
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCEEEEECCCHHHHHHH
KGKSDFSLFSPYLEQLIEFNKRFITYWGYQEHPYDALLDLFEPGVTVKVLDQLFAELKEA
CCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
IIPLVKQVTASGNKPDTSFITKAFPKEKQKELSLYFLQELGYDFDGGRLDETVHPFATTL
HHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHC
NRGDVRVTTRYDEKDFRTAIFGTIHECGHAIYEQNIDEALSGTNLSDGASMGIHESQSLF
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCHHHHHH
YENFIGRNKHFWTPYYKKIQEASPVQFKDISLDDFVRAINESKPSFIRVEADELTYPLHI
HHHHHCCCCCCCCHHHHHHHCCCCCEEECCCHHHHHHHHHCCCCCEEEEEHHHCCCEEEE
IIRYEIEKAIFSNEVSVEDLPSLWNQKYQDYLGITPQTDAEGILQDVHWAGGDFGYFPSY
EEEEHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCHHH
ALGYMYAAQLKQKMLEDLPEFDALLERGEFHPIKQWLTEKVHIHGKRKKPLDIIKDATGE
HHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCC
ELNVRYLIDYLSNKYSNLYLL
CCHHHHHHHHHHCCCCCEEEC
>Mature Secondary Structure
MEIHTYEKEFFDLLKRISHYSEAVALMHWDSRTGAPKNGSEDRAESIGQLSTDIFNIQTS
CCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHEECCCH
DRMKELIDVLYERFDDLSEDTKKAVELAKKEYEENKKIPEAEYKEYVILCSKAETAWEEA
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCEEEEECCCHHHHHHH
KGKSDFSLFSPYLEQLIEFNKRFITYWGYQEHPYDALLDLFEPGVTVKVLDQLFAELKEA
CCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
IIPLVKQVTASGNKPDTSFITKAFPKEKQKELSLYFLQELGYDFDGGRLDETVHPFATTL
HHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHC
NRGDVRVTTRYDEKDFRTAIFGTIHECGHAIYEQNIDEALSGTNLSDGASMGIHESQSLF
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCHHHHHH
YENFIGRNKHFWTPYYKKIQEASPVQFKDISLDDFVRAINESKPSFIRVEADELTYPLHI
HHHHHCCCCCCCCHHHHHHHCCCCCEEECCCHHHHHHHHHCCCCCEEEEEHHHCCCEEEE
IIRYEIEKAIFSNEVSVEDLPSLWNQKYQDYLGITPQTDAEGILQDVHWAGGDFGYFPSY
EEEEHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCHHH
ALGYMYAAQLKQKMLEDLPEFDALLERGEFHPIKQWLTEKVHIHGKRKKPLDIIKDATGE
HHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCC
ELNVRYLIDYLSNKYSNLYLL
CCHHHHHHHHHHCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377