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The map label for this gene is thyA

Identifier: 16079241

GI number: 16079241

Start: 2297106

End: 2297900

Strand: Reverse

Name: thyA

Synonym: BSU21820

Alternate gene names: 16079241

Gene position: 2297900-2297106 (Counterclockwise)

Preceding gene: 16079242

Following gene: 16079240

Centisome position: 54.51

GC content: 44.15

Gene sequence:

>795_bases
ATGAAACAGTATAAGGATTTCTGCAGACATGTTTTAGAGCATGGTGAGAAAAAGGGAGACCGGACTGGGACCGGAACAAT
CAGCACTTTCGGATATCAAATGAGATTTAATTTACGGGAAGGCTTTCCGATGCTCACCACTAAAAAACTCCACTTTAAAT
CAATTGCGCATGAACTGCTGTGGTTCTTAAAAGGAGATACGAATGTACGCTATCTGCAGGAAAACGGAGTGCGAATCTGG
AATGAGTGGGCTGATGAAAACGGTGAACTTGGACCTGTATATGGCTCCCAATGGCGTTCTTGGCGGGGAGCTGATGGAGA
AACCATTGATCAAATTTCCCGTCTTATTGAAGATATTAAAACAAATCCGAACTCCAGACGCTTAATCGTCAGCGCCTGGA
ATGTTGGTGAAATTGATAAAATGGCGTTGCCGCCGTGCCATTGCCTGTTCCAATTCTATGTGTCTGACGGCAAGCTGTCC
TGTCAGCTGTATCAGCGCTCTGCCGATGTTTTCTTAGGTGTGCCGTTTAATATTGCATCTTATGCCCTCCTAACCATGAT
CATTGCTCATGTGACTGGGCTTGAACCGGGCGAGTTCATCCATACGTTTGGTGATGTTCATATTTACCAAAATCATATTG
AACAAGTCAATTTGCAGCTGGAAAGAGATGTTAGACCGCTTCCGCAGCTTCGTTTCGCCAGAAAGGTTGATTCTATTTTT
AACTTTGCATTTGAGGACTTTATCATCGAGGATTATGATCCGCATCCTCATATAAAAGGGGCGGTCAGCGTATGA

Upstream 100 bases:

>100_bases
AGCCTGTCGTCAGCTGACTATAAAAAAATCATTTCTGGGTTCAGAAATGATTTTTTATTGTGTTACACTACTAGAAGACT
ACTTTTAAAGGATGAAAAAA

Downstream 100 bases:

>100_bases
TTTCATTCATTTTTGCGATGGATGCCAACAGGCTTATCGGCAAAGACAATGATTTGCCGTGGCATTTGCCCAATGATCTT
GCATACTTTAAGAAAATAAC

Product: thymidylate synthase

Products: NA

Alternate protein names: TS 2; TSase 2; Thymidylate synthase B; TS B; TSase B [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKQYKDFCRHVLEHGEKKGDRTGTGTISTFGYQMRFNLREGFPMLTTKKLHFKSIAHELLWFLKGDTNVRYLQENGVRIW
NEWADENGELGPVYGSQWRSWRGADGETIDQISRLIEDIKTNPNSRRLIVSAWNVGEIDKMALPPCHCLFQFYVSDGKLS
CQLYQRSADVFLGVPFNIASYALLTMIIAHVTGLEPGEFIHTFGDVHIYQNHIEQVNLQLERDVRPLPQLRFARKVDSIF
NFAFEDFIIEDYDPHPHIKGAVSV

Sequences:

>Translated_264_residues
MKQYKDFCRHVLEHGEKKGDRTGTGTISTFGYQMRFNLREGFPMLTTKKLHFKSIAHELLWFLKGDTNVRYLQENGVRIW
NEWADENGELGPVYGSQWRSWRGADGETIDQISRLIEDIKTNPNSRRLIVSAWNVGEIDKMALPPCHCLFQFYVSDGKLS
CQLYQRSADVFLGVPFNIASYALLTMIIAHVTGLEPGEFIHTFGDVHIYQNHIEQVNLQLERDVRPLPQLRFARKVDSIF
NFAFEDFIIEDYDPHPHIKGAVSV
>Mature_264_residues
MKQYKDFCRHVLEHGEKKGDRTGTGTISTFGYQMRFNLREGFPMLTTKKLHFKSIAHELLWFLKGDTNVRYLQENGVRIW
NEWADENGELGPVYGSQWRSWRGADGETIDQISRLIEDIKTNPNSRRLIVSAWNVGEIDKMALPPCHCLFQFYVSDGKLS
CQLYQRSADVFLGVPFNIASYALLTMIIAHVTGLEPGEFIHTFGDVHIYQNHIEQVNLQLERDVRPLPQLRFARKVDSIF
NFAFEDFIIEDYDPHPHIKGAVSV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=283, Percent_Identity=53.7102473498233, Blast_Score=309, Evalue=2e-84,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=64.0151515151515, Blast_Score=372, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI71993377, Length=283, Percent_Identity=45.2296819787986, Blast_Score=267, Evalue=4e-72,
Organism=Saccharomyces cerevisiae, GI6324648, Length=292, Percent_Identity=42.4657534246575, Blast_Score=220, Evalue=2e-58,
Organism=Drosophila melanogaster, GI17137556, Length=284, Percent_Identity=53.8732394366197, Blast_Score=309, Evalue=1e-84,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 30538; Mature: 30538

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYKDFCRHVLEHGEKKGDRTGTGTISTFGYQMRFNLREGFPMLTTKKLHFKSIAHELL
CCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCEEECHHHHHHHHHHHH
WFLKGDTNVRYLQENGVRIWNEWADENGELGPVYGSQWRSWRGADGETIDQISRLIEDIK
HHHCCCCCEEEEECCCCEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TNPNSRRLIVSAWNVGEIDKMALPPCHCLFQFYVSDGKLSCQLYQRSADVFLGVPFNIAS
CCCCCCEEEEEECCCCCCHHHCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEECCHHHHH
YALLTMIIAHVTGLEPGEFIHTFGDVHIYQNHIEQVNLQLERDVRPLPQLRFARKVDSIF
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
NFAFEDFIIEDYDPHPHIKGAVSV
HHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKQYKDFCRHVLEHGEKKGDRTGTGTISTFGYQMRFNLREGFPMLTTKKLHFKSIAHELL
CCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCEEECHHHHHHHHHHHH
WFLKGDTNVRYLQENGVRIWNEWADENGELGPVYGSQWRSWRGADGETIDQISRLIEDIK
HHHCCCCCEEEEECCCCEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TNPNSRRLIVSAWNVGEIDKMALPPCHCLFQFYVSDGKLSCQLYQRSADVFLGVPFNIAS
CCCCCCEEEEEECCCCCCHHHCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEECCHHHHH
YALLTMIIAHVTGLEPGEFIHTFGDVHIYQNHIEQVNLQLERDVRPLPQLRFARKVDSIF
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
NFAFEDFIIEDYDPHPHIKGAVSV
HHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA