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The map label for this gene is dfrA

Identifier: 16079240

GI number: 16079240

Start: 2296603

End: 2297109

Strand: Reverse

Name: dfrA

Synonym: BSU21810

Alternate gene names: 16079240

Gene position: 2297109-2296603 (Counterclockwise)

Preceding gene: 16079241

Following gene: 255767477

Centisome position: 54.49

GC content: 42.41

Gene sequence:

>507_bases
ATGATTTCATTCATTTTTGCGATGGATGCCAACAGGCTTATCGGCAAAGACAATGATTTGCCGTGGCATTTGCCCAATGA
TCTTGCATACTTTAAGAAAATAACATCGGGCCATTCAATCATTATGGGCCGGAAAACATTTGAATCGATCGGACGTCCGC
TTCCAAATCGGAAAAATATTGTCGTTACCTCAGCGCCGGATTCAGAATTTCAGGGATGCACGGTTGTCAGTTCATTAAAG
GATGTACTGGACATTTGTTCAGGCCCTGAAGAATGCTTTGTGATCGGAGGGGCTCAGCTCTATACGGACCTGTTCCCTTA
TGCGGACAGACTGTATATGACGAAAATTCATCACGAGTTTGAGGGTGACCGTCACTTTCCTGAATTTGATGAATCCAATT
GGAAGCTGGTTTCTTCTGAGCAGGGGACCAAAGACGAAAAAAACCCGTATGATTACGAATTTCTAATGTATGAAAAAAAG
AATTCTTCTAAAGCGGGAGGATTTTAA

Upstream 100 bases:

>100_bases
GTTTCGCCAGAAAGGTTGATTCTATTTTTAACTTTGCATTTGAGGACTTTATCATCGAGGATTATGATCCGCATCCTCAT
ATAAAAGGGGCGGTCAGCGT

Downstream 100 bases:

>100_bases
TTGGTTCGCTACAGCCTTCTAGTGGTTTATATTGTGTATATGCTGTTAAAAAATATGAAACAATTATTTAATCAAACAAT
GCTCGATCCCCGTCTGTCAT

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MISFIFAMDANRLIGKDNDLPWHLPNDLAYFKKITSGHSIIMGRKTFESIGRPLPNRKNIVVTSAPDSEFQGCTVVSSLK
DVLDICSGPEECFVIGGAQLYTDLFPYADRLYMTKIHHEFEGDRHFPEFDESNWKLVSSEQGTKDEKNPYDYEFLMYEKK
NSSKAGGF

Sequences:

>Translated_168_residues
MISFIFAMDANRLIGKDNDLPWHLPNDLAYFKKITSGHSIIMGRKTFESIGRPLPNRKNIVVTSAPDSEFQGCTVVSSLK
DVLDICSGPEECFVIGGAQLYTDLFPYADRLYMTKIHHEFEGDRHFPEFDESNWKLVSSEQGTKDEKNPYDYEFLMYEKK
NSSKAGGF
>Mature_168_residues
MISFIFAMDANRLIGKDNDLPWHLPNDLAYFKKITSGHSIIMGRKTFESIGRPLPNRKNIVVTSAPDSEFQGCTVVSSLK
DVLDICSGPEECFVIGGAQLYTDLFPYADRLYMTKIHHEFEGDRHFPEFDESNWKLVSSEQGTKDEKNPYDYEFLMYEKK
NSSKAGGF

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain

Homologues:

Organism=Homo sapiens, GI4503323, Length=183, Percent_Identity=33.3333333333333, Blast_Score=85, Evalue=3e-17,
Organism=Homo sapiens, GI28827793, Length=163, Percent_Identity=31.9018404907975, Blast_Score=73, Evalue=1e-13,
Organism=Escherichia coli, GI1786233, Length=160, Percent_Identity=43.75, Blast_Score=146, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17505899, Length=182, Percent_Identity=33.5164835164835, Blast_Score=83, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6324810, Length=137, Percent_Identity=30.6569343065693, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI116008026, Length=177, Percent_Identity=29.9435028248588, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24647458, Length=177, Percent_Identity=29.9435028248588, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): DYR_BACSU (P11045)

Other databases:

- EMBL:   M20012
- EMBL:   L77246
- EMBL:   AL009126
- PIR:   JT0291
- RefSeq:   NP_390064.1
- ProteinModelPortal:   P11045
- SMR:   P11045
- EnsemblBacteria:   EBBACT00000004064
- GeneID:   939091
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU21810
- NMPDR:   fig|224308.1.peg.2187
- GenoList:   BSU21810
- GeneTree:   EBGT00050000002709
- HOGENOM:   HBG665708
- OMA:   APNTFGY
- PhylomeDB:   P11045
- ProtClustDB:   CLSK2502627
- BioCyc:   BSUB:BSU21810-MONOMER
- BRENDA:   1.5.1.3
- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796
- PANTHER:   PTHR11549:SF1
- PRINTS:   PR00070

Pfam domain/function: PF00186 DHFR_1

EC number: =1.5.1.3

Molecular weight: Translated: 19176; Mature: 19176

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00075 DHFR_1; PS51330 DHFR_2

Important sites: BINDING 27-27 BINDING 57-57 BINDING 114-114

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISFIFAMDANRLIGKDNDLPWHLPNDLAYFKKITSGHSIIMGRKTFESIGRPLPNRKNI
CEEEEEEECCCHHCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCCCE
VVTSAPDSEFQGCTVVSSLKDVLDICSGPEECFVIGGAQLYTDLFPYADRLYMTKIHHEF
EEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHC
EGDRHFPEFDESNWKLVSSEQGTKDEKNPYDYEFLMYEKKNSSKAGGF
CCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MISFIFAMDANRLIGKDNDLPWHLPNDLAYFKKITSGHSIIMGRKTFESIGRPLPNRKNI
CEEEEEEECCCHHCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCCCE
VVTSAPDSEFQGCTVVSSLKDVLDICSGPEECFVIGGAQLYTDLFPYADRLYMTKIHHEF
EEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHC
EGDRHFPEFDESNWKLVSSEQGTKDEKNPYDYEFLMYEKKNSSKAGGF
CCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840350; 9384377