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The map label for this gene is ligB

Identifier: 16079109

GI number: 16079109

Start: 2192370

End: 2193182

Strand: Direct

Name: ligB

Synonym: BSU20500

Alternate gene names: 16079109

Gene position: 2192370-2193182 (Clockwise)

Preceding gene: 16079108

Following gene: 16079111

Centisome position: 52.01

GC content: 37.88

Gene sequence:

>813_bases
TTGTTTGTATCGCCAATGCTATTGCATTCAATAAAAGAGCCTTTTAACGACGAGAACTATATCACTGAGCTCAAATTTGA
TGGAATCAGACTCATCCTTTCTAAATTTGATAATCAGATTAAGCTATACACTCGTCACAACAACGAAGTAACAAGTAAGT
TCCCAGAACTCATGGACTTAGATATTCCAGATGGAACAGTGCTCGATGGTGAAGTTATTGTAGCTGCCTCAGGTGGTGCT
CCTGACTTTGAGGCTGTAATGGAACGTTTTATGTCCAAAAAATCAGCTCATAATATTGTTTACTGTGTCTTTGATGTTAT
CTACAAAGACGGTCAATCAATTGCAGCTAAGCCACTCACTGAGCGTAAGACAGTTCTGAATTCACTTGAACTGGATCATC
CTAATGTCTTTGTTATAGAGGGAACTCAAGGTAACGGTCTTGCTTACTTCAACCTGGCCAAAGAAAAGAATTTAGAGGGA
ATCGTAATCAAGAAAGCTAACTCCCCTTATGAGATCAATAAACGTTCGCATAATTGGCTTAAAGTGATTAATTACGATTA
CACTGAGGTTCTCATAACTGGCTACACCAAAAAGGATATAAAATTCCTTCTGTCTTATCCTGATGGAACTTCAGCTGGAT
TTATGGAATTTATGCCACATGCAGAACGAACTAAGTTCCACTCTATGAAACAAGTTAAGTCTGAATCTGATGAATATGTA
TTTATCGAACCTATCTTATGTAACGTTAAGCACAGATTTAAGACTAAGCACGGTAAACTCCGCATACCTTCCTTTGAATC
CTGGAGAGTGTAA

Upstream 100 bases:

>100_bases
CAATTCACCTGAGCTCATTGAATCCCATTAAGTACCACTGCCATATCGCTTTATATATCACCTTCGCTTAGCTAATATGT
TCTAAGTAGGAGGTGATATT

Downstream 100 bases:

>100_bases
TCTCTCCGTTACATAACTTCTTAGAGCCTAGCCTTTTAAAATTCAATGAAAATTTAGTACATGGATCAATTAGTATTCAT
CCATTCATCGAAGCTATAAG

Product: ATP-dependent DNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MFVSPMLLHSIKEPFNDENYITELKFDGIRLILSKFDNQIKLYTRHNNEVTSKFPELMDLDIPDGTVLDGEVIVAASGGA
PDFEAVMERFMSKKSAHNIVYCVFDVIYKDGQSIAAKPLTERKTVLNSLELDHPNVFVIEGTQGNGLAYFNLAKEKNLEG
IVIKKANSPYEINKRSHNWLKVINYDYTEVLITGYTKKDIKFLLSYPDGTSAGFMEFMPHAERTKFHSMKQVKSESDEYV
FIEPILCNVKHRFKTKHGKLRIPSFESWRV

Sequences:

>Translated_270_residues
MFVSPMLLHSIKEPFNDENYITELKFDGIRLILSKFDNQIKLYTRHNNEVTSKFPELMDLDIPDGTVLDGEVIVAASGGA
PDFEAVMERFMSKKSAHNIVYCVFDVIYKDGQSIAAKPLTERKTVLNSLELDHPNVFVIEGTQGNGLAYFNLAKEKNLEG
IVIKKANSPYEINKRSHNWLKVINYDYTEVLITGYTKKDIKFLLSYPDGTSAGFMEFMPHAERTKFHSMKQVKSESDEYV
FIEPILCNVKHRFKTKHGKLRIPSFESWRV
>Mature_270_residues
MFVSPMLLHSIKEPFNDENYITELKFDGIRLILSKFDNQIKLYTRHNNEVTSKFPELMDLDIPDGTVLDGEVIVAASGGA
PDFEAVMERFMSKKSAHNIVYCVFDVIYKDGQSIAAKPLTERKTVLNSLELDHPNVFVIEGTQGNGLAYFNLAKEKNLEG
IVIKKANSPYEINKRSHNWLKVINYDYTEVLITGYTKKDIKFLLSYPDGTSAGFMEFMPHAERTKFHSMKQVKSESDEYV
FIEPILCNVKHRFKTKHGKLRIPSFESWRV

Specific function: Unknown

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family

Homologues:

Organism=Homo sapiens, GI4557719, Length=213, Percent_Identity=26.7605633802817, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71985269, Length=212, Percent_Identity=28.7735849056604, Blast_Score=70, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIGB_BACSU (O31917)

Other databases:

- EMBL:   AL009126
- RefSeq:   NP_389932.1
- ProteinModelPortal:   O31917
- SMR:   O31917
- EnsemblBacteria:   EBBACT00000003206
- GeneID:   939992
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU20500
- NMPDR:   fig|224308.1.peg.2055
- GenoList:   BSU20500
- GeneTree:   EBGT00050000000592
- HOGENOM:   HBG344688
- OMA:   FDGIRLI
- ProtClustDB:   PRK07636
- BioCyc:   BSUB:BSU20500-MONOMER
- InterPro:   IPR012310

Pfam domain/function: PF01068 DNA_ligase_A_M

EC number: NA

Molecular weight: Translated: 31027; Mature: 31027

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: PS50160 DNA_LIGASE_A3

Important sites: ACT_SITE 25-25

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVSPMLLHSIKEPFNDENYITELKFDGIRLILSKFDNQIKLYTRHNNEVTSKFPELMDL
CCCCHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHCCCEEEEEEECCCCHHHHCCHHHCC
DIPDGTVLDGEVIVAASGGAPDFEAVMERFMSKKSAHNIVYCVFDVIYKDGQSIAAKPLT
CCCCCEEECCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHCCCCCCCCCCH
ERKTVLNSLELDHPNVFVIEGTQGNGLAYFNLAKEKNLEGIVIKKANSPYEINKRSHNWL
HHHHHHHHHCCCCCCEEEEECCCCCCEEEEEECCCCCCCEEEEEECCCCEEECCCCCCEE
KVINYDYTEVLITGYTKKDIKFLLSYPDGTSAGFMEFMPHAERTKFHSMKQVKSESDEYV
EEEECCCEEEEEECCCCCCEEEEEECCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCCEE
FIEPILCNVKHRFKTKHGKLRIPSFESWRV
EEEHEEECCHHHHCCCCCCEECCCCCCCCC
>Mature Secondary Structure
MFVSPMLLHSIKEPFNDENYITELKFDGIRLILSKFDNQIKLYTRHNNEVTSKFPELMDL
CCCCHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHCCCEEEEEEECCCCHHHHCCHHHCC
DIPDGTVLDGEVIVAASGGAPDFEAVMERFMSKKSAHNIVYCVFDVIYKDGQSIAAKPLT
CCCCCEEECCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHCCCCCCCCCCH
ERKTVLNSLELDHPNVFVIEGTQGNGLAYFNLAKEKNLEGIVIKKANSPYEINKRSHNWL
HHHHHHHHHCCCCCCEEEEECCCCCCEEEEEECCCCCCCEEEEEECCCCEEECCCCCCEE
KVINYDYTEVLITGYTKKDIKFLLSYPDGTSAGFMEFMPHAERTKFHSMKQVKSESDEYV
EEEECCCEEEEEECCCCCCEEEEEECCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCCEE
FIEPILCNVKHRFKTKHGKLRIPSFESWRV
EEEHEEECCHHHHCCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377