Click here to switch to the map view.

The map label for this gene is yocI

Identifier: 16078982

GI number: 16078982

Start: 2094010

End: 2095785

Strand: Reverse

Name: yocI

Synonym: BSU19220

Alternate gene names: 16078982

Gene position: 2095785-2094010 (Counterclockwise)

Preceding gene: 16078983

Following gene: 16078981

Centisome position: 49.71

GC content: 43.41

Gene sequence:

>1776_bases
ATGTTACATAGAGCCCAATCCCTTCTGGCTCATTATTTTGGTTATGAGAAGTTTCGGAGCGGCCAAGATGAAGCGATCCG
CTTGGTGACGGAAGCGAGGCAAAATACGGCCTGCATTATGCCGACGGGAGGCGGCAAATCGATTTGCTATCAGATTCCGG
CATTAATGTTTGAAGGGACGACGATCGTCATTTCACCGTTGATTTCCTTGATGAAGGATCAAGTTGATGCGTTAGAAGAA
GCGGGGATTAACGCCGCCTACATTAACAGCACACAATCCAATCAGGAAATATATGAGAGGCTGAACGGACTCAAAGAAGG
GGCTTATAAGCTTTTTTACATTACACCAGAGCGACTAACGTCCATAGAGTTTATCCGGATTTTGCAGGGCATAGATGTTC
CTTTGGTCGCAATAGACGAGGCGCATTGTATTTCTCAATGGGGCCATGATTTCAGGCCAAGCTACAGGAATATTGAAATC
TTGTTCCGTGAATTACACGACAAGCCTGTCATCATGGCGTTAACAGCAACAGCCACACCCGAGGTTCATGATGATATTTG
CAAACAGCTGCATATTCAAAAAGAAAACACGGTATATACCGGATTTTCCCGAGAGAATTTAACCTTTAAAGTAGTAAAAG
GAGAAAATAAAGATCGGTTTATAGACGAATATGTGCAAAACAACAGGCATGAAGCAGGAATTGTCTATACAGCGACCAGA
AAAGAAGCTGATCGGATATATGAGAGATTGAAACGAAATCAGGTCAGGGCAGGCCGGTATCATGGAGGTTTGGCTGATGA
TGTGAGAAAAGAGCAGCAGGAAAGATTTCTTAATGATGAGCTTCAAGTGATGGTGGCAACCTCTGCTTTTGGAATGGGTA
TTGATAAATCAAATATCAGATTTGTTCTGCATGCACAAATCCCGAAAGATATGGAAAGCTATTATCAAGAGGCAGGACGA
GCTGGACGGGACGGTCTTGCCAGTGAATGTGTTCTCCTGTTTTCACCGCAGGATATTATGGTGCAGCGCTTCTTAATTGA
GCAATCAGAACATGAAGAGAAACAAAAGCAGGATTTAAAAAAGCTGAGACAAATGGTCGACTACTGTCATACGGAAGATT
GTCTGCAGCGTTTTATCCTTATGTATTTCGGAGAAAAGGAGCCTGATGCGTGCGGACAGTGCGGAAACTGTACCGATACA
AGAGCGGCCCATGACGTGACGCGGGAGGCGCAAATGGTCTTATCTTGTATCATTCGCATGAAAGAACGGTTTGGCAAGAC
GATGGTTGCCCAGGTGCTTGCCGGCTCCAAAAATAAAAAAGTTCTTGAAAACGGCTTTTCCGACCTTTCAACATACGGGA
TATTAAAACACCAATCCGTTGGCGAAATCAGTGATTTTATCGAATTTCTTATTTCTGACGACTTCATCAGGATGTCAGAC
GGAACGTTTCCGACTCTTTTTGTCAGCAGTAAAGGAAGGAATGTGCTGAAAGGAGAATTATCAGTAGCGAGAAAAGAAGC
GTTAAAAGCGGCAGCGATTACTGAAAATGACGAGCTATTTGAACGATTGCGCATGGTTCGGAAAGAAATTGCGGCTGAGC
AGGGTGTTCCGCCATTTGTTGTTTTCTCTGACCAAACGTTAAAAGAAATGTCAGGAAAACAGCCCGTAAATGATGATGAA
CTTTTGTCTATAAAAGGAGTCGGAGAACAAAAAAGAGCAAAATACGGCCGGCTTTTTTTACAAGAAATACAGGCTTATGC
AAGGATGACAGACTAA

Upstream 100 bases:

>100_bases
GTTTGGTATAGTGAATATTCCATTTTTGTGTGAAAGAAATGAAGGCCGCTTTTTTATGATATGATGGGAGAAATTCAAGG
AAAAGCGAGGTTTACACGTT

Downstream 100 bases:

>100_bases
AAAAACAGAAAAACAATCTTTTTTTCTATAAAAAACACATTTTATTTTCTCTGTTTCGCCTCAAAATAAAAAGACCTGCG
AAGCCTATTATATCAAGGGT

Product: ATP-dependent nucleic acid helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MLHRAQSLLAHYFGYEKFRSGQDEAIRLVTEARQNTACIMPTGGGKSICYQIPALMFEGTTIVISPLISLMKDQVDALEE
AGINAAYINSTQSNQEIYERLNGLKEGAYKLFYITPERLTSIEFIRILQGIDVPLVAIDEAHCISQWGHDFRPSYRNIEI
LFRELHDKPVIMALTATATPEVHDDICKQLHIQKENTVYTGFSRENLTFKVVKGENKDRFIDEYVQNNRHEAGIVYTATR
KEADRIYERLKRNQVRAGRYHGGLADDVRKEQQERFLNDELQVMVATSAFGMGIDKSNIRFVLHAQIPKDMESYYQEAGR
AGRDGLASECVLLFSPQDIMVQRFLIEQSEHEEKQKQDLKKLRQMVDYCHTEDCLQRFILMYFGEKEPDACGQCGNCTDT
RAAHDVTREAQMVLSCIIRMKERFGKTMVAQVLAGSKNKKVLENGFSDLSTYGILKHQSVGEISDFIEFLISDDFIRMSD
GTFPTLFVSSKGRNVLKGELSVARKEALKAAAITENDELFERLRMVRKEIAAEQGVPPFVVFSDQTLKEMSGKQPVNDDE
LLSIKGVGEQKRAKYGRLFLQEIQAYARMTD

Sequences:

>Translated_591_residues
MLHRAQSLLAHYFGYEKFRSGQDEAIRLVTEARQNTACIMPTGGGKSICYQIPALMFEGTTIVISPLISLMKDQVDALEE
AGINAAYINSTQSNQEIYERLNGLKEGAYKLFYITPERLTSIEFIRILQGIDVPLVAIDEAHCISQWGHDFRPSYRNIEI
LFRELHDKPVIMALTATATPEVHDDICKQLHIQKENTVYTGFSRENLTFKVVKGENKDRFIDEYVQNNRHEAGIVYTATR
KEADRIYERLKRNQVRAGRYHGGLADDVRKEQQERFLNDELQVMVATSAFGMGIDKSNIRFVLHAQIPKDMESYYQEAGR
AGRDGLASECVLLFSPQDIMVQRFLIEQSEHEEKQKQDLKKLRQMVDYCHTEDCLQRFILMYFGEKEPDACGQCGNCTDT
RAAHDVTREAQMVLSCIIRMKERFGKTMVAQVLAGSKNKKVLENGFSDLSTYGILKHQSVGEISDFIEFLISDDFIRMSD
GTFPTLFVSSKGRNVLKGELSVARKEALKAAAITENDELFERLRMVRKEIAAEQGVPPFVVFSDQTLKEMSGKQPVNDDE
LLSIKGVGEQKRAKYGRLFLQEIQAYARMTD
>Mature_591_residues
MLHRAQSLLAHYFGYEKFRSGQDEAIRLVTEARQNTACIMPTGGGKSICYQIPALMFEGTTIVISPLISLMKDQVDALEE
AGINAAYINSTQSNQEIYERLNGLKEGAYKLFYITPERLTSIEFIRILQGIDVPLVAIDEAHCISQWGHDFRPSYRNIEI
LFRELHDKPVIMALTATATPEVHDDICKQLHIQKENTVYTGFSRENLTFKVVKGENKDRFIDEYVQNNRHEAGIVYTATR
KEADRIYERLKRNQVRAGRYHGGLADDVRKEQQERFLNDELQVMVATSAFGMGIDKSNIRFVLHAQIPKDMESYYQEAGR
AGRDGLASECVLLFSPQDIMVQRFLIEQSEHEEKQKQDLKKLRQMVDYCHTEDCLQRFILMYFGEKEPDACGQCGNCTDT
RAAHDVTREAQMVLSCIIRMKERFGKTMVAQVLAGSKNKKVLENGFSDLSTYGILKHQSVGEISDFIEFLISDDFIRMSD
GTFPTLFVSSKGRNVLKGELSVARKEALKAAAITENDELFERLRMVRKEIAAEQGVPPFVVFSDQTLKEMSGKQPVNDDE
LLSIKGVGEQKRAKYGRLFLQEIQAYARMTD

Specific function: Probable DNA helicase. Required for DNA repair and intramolecular recombination; probably has overlapping function with recS (AC P50729). It probably acts to help generate ss-DNA from ds-DNA breaks

COG id: COG0514

COG function: function code L; Superfamily II DNA helicase

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Localized throughout the nucleoid in the presence or absence of DNA double- strand breaks

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HRDC domain

Homologues:

Organism=Homo sapiens, GI4557365, Length=633, Percent_Identity=34.913112164297, Blast_Score=330, Evalue=2e-90,
Organism=Homo sapiens, GI110735439, Length=592, Percent_Identity=33.1081081081081, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI169218196, Length=592, Percent_Identity=33.1081081081081, Blast_Score=290, Evalue=4e-78,
Organism=Homo sapiens, GI14591902, Length=416, Percent_Identity=38.7019230769231, Blast_Score=267, Evalue=2e-71,
Organism=Homo sapiens, GI14591904, Length=416, Percent_Identity=38.7019230769231, Blast_Score=267, Evalue=2e-71,
Organism=Homo sapiens, GI148612816, Length=445, Percent_Identity=35.9550561797753, Blast_Score=257, Evalue=3e-68,
Organism=Homo sapiens, GI51242943, Length=393, Percent_Identity=38.1679389312977, Blast_Score=246, Evalue=4e-65,
Organism=Homo sapiens, GI51242947, Length=393, Percent_Identity=38.1679389312977, Blast_Score=246, Evalue=6e-65,
Organism=Homo sapiens, GI284005309, Length=348, Percent_Identity=33.6206896551724, Blast_Score=197, Evalue=2e-50,
Organism=Escherichia coli, GI48994977, Length=592, Percent_Identity=40.2027027027027, Blast_Score=447, Evalue=1e-127,
Organism=Escherichia coli, GI87082221, Length=336, Percent_Identity=23.5119047619048, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25145561, Length=652, Percent_Identity=30.9815950920245, Blast_Score=288, Evalue=6e-78,
Organism=Caenorhabditis elegans, GI71987997, Length=511, Percent_Identity=33.6594911937378, Blast_Score=277, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI71987993, Length=511, Percent_Identity=33.6594911937378, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI32564293, Length=479, Percent_Identity=35.9081419624217, Blast_Score=266, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI17552866, Length=441, Percent_Identity=33.1065759637188, Blast_Score=237, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI17507945, Length=314, Percent_Identity=23.8853503184713, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323844, Length=530, Percent_Identity=33.3962264150943, Blast_Score=293, Evalue=7e-80,
Organism=Drosophila melanogaster, GI24646066, Length=633, Percent_Identity=33.9652448657188, Blast_Score=316, Evalue=3e-86,
Organism=Drosophila melanogaster, GI24664226, Length=428, Percent_Identity=35.7476635514019, Blast_Score=259, Evalue=4e-69,
Organism=Drosophila melanogaster, GI62472181, Length=428, Percent_Identity=35.7476635514019, Blast_Score=258, Evalue=9e-69,
Organism=Drosophila melanogaster, GI24664230, Length=428, Percent_Identity=35.7476635514019, Blast_Score=258, Evalue=9e-69,
Organism=Drosophila melanogaster, GI21358123, Length=356, Percent_Identity=35.3932584269663, Blast_Score=201, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECQ_BACSU (O34748)

Other databases:

- EMBL:   AF027868
- EMBL:   AL009126
- PIR:   F69901
- RefSeq:   NP_389803.1
- HSSP:   P15043
- ProteinModelPortal:   O34748
- EnsemblBacteria:   EBBACT00000001699
- GeneID:   939671
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU19220
- NMPDR:   fig|224308.1.peg.1926
- GenoList:   BSU19220
- GeneTree:   EBGT00070000031730
- HOGENOM:   HBG651799
- OMA:   PSCKLLY
- PhylomeDB:   O34748
- ProtClustDB:   CLSK887416
- BioCyc:   BSUB:BSU19220-MONOMER
- GO:   GO:0005737
- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004589
- InterPro:   IPR006293
- InterPro:   IPR002121
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR010997
- InterPro:   IPR018982
- PANTHER:   PTHR13710
- SMART:   SM00487
- SMART:   SM00490
- SMART:   SM00341
- SMART:   SM00956
- TIGRFAMs:   TIGR01389
- TIGRFAMs:   TIGR00614

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C; PF00570 HRDC; PF09382 RQC; SSF47819 HRDC_like

EC number: =3.6.4.12

Molecular weight: Translated: 67354; Mature: 67354

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00690 DEAH_ATP_HELICASE; PS51192 HELICASE_ATP_BIND_1; PS51194 HELICASE_CTER; PS50967 HRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHRAQSLLAHYFGYEKFRSGQDEAIRLVTEARQNTACIMPTGGGKSICYQIPALMFEGT
CCCHHHHHHHHHHCHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCC
TIVISPLISLMKDQVDALEEAGINAAYINSTQSNQEIYERLNGLKEGAYKLFYITPERLT
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECHHHHH
SIEFIRILQGIDVPLVAIDEAHCISQWGHDFRPSYRNIEILFRELHDKPVIMALTATATP
HHHHHHHHHCCCCCEEEECCHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCC
EVHDDICKQLHIQKENTVYTGFSRENLTFKVVKGENKDRFIDEYVQNNRHEAGIVYTATR
HHHHHHHHHHCCCCCCCEEECCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCEEEEECH
KEADRIYERLKRNQVRAGRYHGGLADDVRKEQQERFLNDELQVMVATSAFGMGIDKSNIR
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHEEEEEEHHHCCCCCCCCCE
FVLHAQIPKDMESYYQEAGRAGRDGLASECVLLFSPQDIMVQRFLIEQSEHEEKQKQDLK
EEEEECCCHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
KLRQMVDYCHTEDCLQRFILMYFGEKEPDACGQCGNCTDTRAAHDVTREAQMVLSCIIRM
HHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
KERFGKTMVAQVLAGSKNKKVLENGFSDLSTYGILKHQSVGEISDFIEFLISDDFIRMSD
HHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECC
GTFPTLFVSSKGRNVLKGELSVARKEALKAAAITENDELFERLRMVRKEIAAEQGVPPFV
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEE
VFSDQTLKEMSGKQPVNDDELLSIKGVGEQKRAKYGRLFLQEIQAYARMTD
EECCHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLHRAQSLLAHYFGYEKFRSGQDEAIRLVTEARQNTACIMPTGGGKSICYQIPALMFEGT
CCCHHHHHHHHHHCHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCC
TIVISPLISLMKDQVDALEEAGINAAYINSTQSNQEIYERLNGLKEGAYKLFYITPERLT
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECHHHHH
SIEFIRILQGIDVPLVAIDEAHCISQWGHDFRPSYRNIEILFRELHDKPVIMALTATATP
HHHHHHHHHCCCCCEEEECCHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCC
EVHDDICKQLHIQKENTVYTGFSRENLTFKVVKGENKDRFIDEYVQNNRHEAGIVYTATR
HHHHHHHHHHCCCCCCCEEECCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCEEEEECH
KEADRIYERLKRNQVRAGRYHGGLADDVRKEQQERFLNDELQVMVATSAFGMGIDKSNIR
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHEEEEEEHHHCCCCCCCCCE
FVLHAQIPKDMESYYQEAGRAGRDGLASECVLLFSPQDIMVQRFLIEQSEHEEKQKQDLK
EEEEECCCHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
KLRQMVDYCHTEDCLQRFILMYFGEKEPDACGQCGNCTDTRAAHDVTREAQMVLSCIIRM
HHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
KERFGKTMVAQVLAGSKNKKVLENGFSDLSTYGILKHQSVGEISDFIEFLISDDFIRMSD
HHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECC
GTFPTLFVSSKGRNVLKGELSVARKEALKAAAITENDELFERLRMVRKEIAAEQGVPPFV
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEE
VFSDQTLKEMSGKQPVNDDELLSIKGVGEQKRAKYGRLFLQEIQAYARMTD
EECCHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 9642195