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The map label for this gene is yocJ

Identifier: 16078983

GI number: 16078983

Start: 2096350

End: 2096976

Strand: Reverse

Name: yocJ

Synonym: BSU19230

Alternate gene names: 16078983

Gene position: 2096976-2096350 (Counterclockwise)

Preceding gene: 255767450

Following gene: 16078982

Centisome position: 49.74

GC content: 40.51

Gene sequence:

>627_bases
ATGTCTACAGTTTTATTTGTAAAATCAAGCGACCGTACAGCTGAAGAAGGCGTTTCAACTAAACTTTACGAAGCTTTCTT
AGCTGCTTATAAAGAAAACAACCCTAATGATGAAGTGGTTGAATTAGATCTTCATAAGGAAAACCTTCCTTACCTTGGCA
GAGATATGATTAACGGAACATTTAAAGCAGGTCAAGGAATGGAAATGACAGAAGATGAGAAAAAACAAGCAGCAATTGCT
GACAAATATCTGAACCAGTTTGTAAAAGCTGACAAAGTTGTTTTCGCATTCCCGCTTTGGAACTTCACAGTGCCAGCAGT
GCTTCATACTTATGTTGATTATCTGTCTCGCGCAGGCGTTACATTCAAATACACACAAGAAGGACCAGTCGGTTTAATGG
GCGGCAAAAAAGTTGCGCTTCTTAACGCTCGCGGCGGTGTCTACTCAGAAGGACCAATGGCTGCACTTGAAATGTCATTA
AACTTCATGAAAACAGTTCTTGGTTTCTGGGGTGTTCAAGACTTGCACACAGTTGTCATCGAAGGACATAACGCAGCACC
TGATCAAGCGCAAGAAATCGTTGAAAAAGGTTTACAAGAAGCAAAAGATCTTGCTGCAAAATTCTAA

Upstream 100 bases:

>100_bases
AAAAATCTCATATTCAAAATAAAGTGATTGACTTTACGTAAAAAAGAAAGTAACATACTATTTGTAAGTAAATATAAAAA
GTTTACCGGGAGGAATACAA

Downstream 100 bases:

>100_bases
TAAAGCCAAGACTCCTATCGTTACAAATAGGAGTCTTTTTGTATATACACATTAATATAGAAGAAAATAATCATTCGCTT
TTAGCAATAAAGGCTATTTT

Product: azoreductase

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase 1; FMN-dependent NADH-azo compound oxidoreductase 1

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MSTVLFVKSSDRTAEEGVSTKLYEAFLAAYKENNPNDEVVELDLHKENLPYLGRDMINGTFKAGQGMEMTEDEKKQAAIA
DKYLNQFVKADKVVFAFPLWNFTVPAVLHTYVDYLSRAGVTFKYTQEGPVGLMGGKKVALLNARGGVYSEGPMAALEMSL
NFMKTVLGFWGVQDLHTVVIEGHNAAPDQAQEIVEKGLQEAKDLAAKF

Sequences:

>Translated_208_residues
MSTVLFVKSSDRTAEEGVSTKLYEAFLAAYKENNPNDEVVELDLHKENLPYLGRDMINGTFKAGQGMEMTEDEKKQAAIA
DKYLNQFVKADKVVFAFPLWNFTVPAVLHTYVDYLSRAGVTFKYTQEGPVGLMGGKKVALLNARGGVYSEGPMAALEMSL
NFMKTVLGFWGVQDLHTVVIEGHNAAPDQAQEIVEKGLQEAKDLAAKF
>Mature_207_residues
STVLFVKSSDRTAEEGVSTKLYEAFLAAYKENNPNDEVVELDLHKENLPYLGRDMINGTFKAGQGMEMTEDEKKQAAIAD
KYLNQFVKADKVVFAFPLWNFTVPAVLHTYVDYLSRAGVTFKYTQEGPVGLMGGKKVALLNARGGVYSEGPMAALEMSLN
FMKTVLGFWGVQDLHTVVIEGHNAAPDQAQEIVEKGLQEAKDLAAKF

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity. Confers resistance to catechol, 2-methylhydroquinone (2-MHQ), and diamide. Probably co

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family

Homologues:

Organism=Escherichia coli, GI1787680, Length=190, Percent_Identity=32.6315789473684, Blast_Score=110, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AZOR1_BACSU (O35022)

Other databases:

- EMBL:   AF027868
- EMBL:   AL009126
- PIR:   G69901
- RefSeq:   NP_389804.1
- ProteinModelPortal:   O35022
- SMR:   O35022
- EnsemblBacteria:   EBBACT00000002043
- GeneID:   939669
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU19230
- NMPDR:   fig|224308.1.peg.1927
- GenoList:   BSU19230
- GeneTree:   EBGT00050000000772
- HOGENOM:   HBG565834
- OMA:   MATVLFV
- PhylomeDB:   O35022
- ProtClustDB:   PRK13556
- BioCyc:   BSUB:BSU19230-MONOMER
- HAMAP:   MF_01216
- InterPro:   IPR003680
- InterPro:   IPR023048

Pfam domain/function: PF02525 Flavodoxin_2

EC number: 3.1.4.14

Molecular weight: Translated: 22977; Mature: 22846

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVLFVKSSDRTAEEGVSTKLYEAFLAAYKENNPNDEVVELDLHKENLPYLGRDMINGT
CCEEEEEECCCCCHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHCCC
FKAGQGMEMTEDEKKQAAIADKYLNQFVKADKVVFAFPLWNFTVPAVLHTYVDYLSRAGV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHCCC
TFKYTQEGPVGLMGGKKVALLNARGGVYSEGPMAALEMSLNFMKTVLGFWGVQDLHTVVI
EEEECCCCCEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHEEEEE
EGHNAAPDQAQEIVEKGLQEAKDLAAKF
ECCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STVLFVKSSDRTAEEGVSTKLYEAFLAAYKENNPNDEVVELDLHKENLPYLGRDMINGT
CEEEEEECCCCCHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHCCC
FKAGQGMEMTEDEKKQAAIADKYLNQFVKADKVVFAFPLWNFTVPAVLHTYVDYLSRAGV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHCCC
TFKYTQEGPVGLMGGKKVALLNARGGVYSEGPMAALEMSLNFMKTVLGFWGVQDLHTVVI
EEEECCCCCEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHEEEEE
EGHNAAPDQAQEIVEKGLQEAKDLAAKF
ECCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377