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The map label for this gene is pelB

Identifier: 16078925

GI number: 16078925

Start: 2034745

End: 2035782

Strand: Direct

Name: pelB

Synonym: BSU18650

Alternate gene names: 16078925

Gene position: 2034745-2035782 (Clockwise)

Preceding gene: 255767439

Following gene: 16078926

Centisome position: 48.27

GC content: 38.82

Gene sequence:

>1038_bases
ATGAAACGACTTTGTTTATGGTTCACCGTCTTTAGCTTGTTTTTGGTTCTCTTGCCTGGAAAAGCATTAGGTGCAGTCGA
TTTTCCGAATACCTCAACAAATGGCCTTTTAGGTTTTGCCGGAAATGCCAAAAATGAAAAAGGCATCTCTAAAACGGGCA
CAACGGGTGGAAAAAACGGGCAGATTGTTTACATTCAAAGCGTAAATGATTTAAAAACCCATCTAGGAGGATCCACTCCG
AAAATTTTAGTGCTTCAAAATGATCTTTCCGCATCTGCGAAAACAACAGTTACCATTGGCTCAAATAAAACACTCGTGGG
CTCTTACGCGAAAAAAACGCTGAAAAATATTTATTTAACAACTTCATCAGCTTTTGGAAATGTCATTTTTCAAAATTTAA
CGTTTGAACACAGTCCGCAGATTAACGGAAATAATGATATCCAGCTTTATTTAGATTCCGGTATGAACTATTGGATTGAT
CACGTCACATTCTCAGGTCATAGCTATAATGCAAGCGGAAGCGATTTGGACAAACTCGTGTATATCGGAAAATCCGCAGA
TTATATCACGATCAGCAACTCAAAATTTGCGAATCATAAATACGGTTTGATTTTAGGATACCCGGATGATTCTCAGCATC
AATATGACGGCTACCCCCACATGACAATCGCCAATAATTATTTTGAGAACTTGTATGTTAGAGGCCCAGGTCTGATGAGA
TACGGTTACTTTCATGTTAAAAACAATTACAGCAATAATTTTAACCAGGCGATTACAATTGCTACAAAAGCAAAAATATA
TTCCGAATATAATTACTTTGGGAAAGGCAGTGAAAAAGGCGGCATCCTTGACGACAAGGGCACTGGTTATTTCAAAGACA
TAGGCAGCTATCCATCACTCAACAAGCAAACCTCTCCGCTGACTTCTTGGAATCCTGGGTCAAACTATAGCTATCGTGTC
CAAACGCCGGAATATACAAAAGAATTTGTCACGAAATATGCCGGATCACAAAGTACAACTCTGGTATTTGGCTACTGA

Upstream 100 bases:

>100_bases
CCAACATTAATTAGGAAGATAGAACCTATTATTCCTTTCCATTAACTGTAATAATAATCCCCGCAAGGAAATGATTAAAA
TTGAAAGGAAGCATATTGTG

Downstream 100 bases:

>100_bases
TATAAAAGAGGCCCTGCGTTAAAAACGCTGTGCCTCTTTTTATACTATAGGCTATTTTTAAGCGATCCTTGTAATTCCAT
CAAGATCCAGCGTCGGTCAA

Product: pectin lyase

Products: NA

Alternate protein names: PNL

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MKRLCLWFTVFSLFLVLLPGKALGAVDFPNTSTNGLLGFAGNAKNEKGISKTGTTGGKNGQIVYIQSVNDLKTHLGGSTP
KILVLQNDLSASAKTTVTIGSNKTLVGSYAKKTLKNIYLTTSSAFGNVIFQNLTFEHSPQINGNNDIQLYLDSGMNYWID
HVTFSGHSYNASGSDLDKLVYIGKSADYITISNSKFANHKYGLILGYPDDSQHQYDGYPHMTIANNYFENLYVRGPGLMR
YGYFHVKNNYSNNFNQAITIATKAKIYSEYNYFGKGSEKGGILDDKGTGYFKDIGSYPSLNKQTSPLTSWNPGSNYSYRV
QTPEYTKEFVTKYAGSQSTTLVFGY

Sequences:

>Translated_345_residues
MKRLCLWFTVFSLFLVLLPGKALGAVDFPNTSTNGLLGFAGNAKNEKGISKTGTTGGKNGQIVYIQSVNDLKTHLGGSTP
KILVLQNDLSASAKTTVTIGSNKTLVGSYAKKTLKNIYLTTSSAFGNVIFQNLTFEHSPQINGNNDIQLYLDSGMNYWID
HVTFSGHSYNASGSDLDKLVYIGKSADYITISNSKFANHKYGLILGYPDDSQHQYDGYPHMTIANNYFENLYVRGPGLMR
YGYFHVKNNYSNNFNQAITIATKAKIYSEYNYFGKGSEKGGILDDKGTGYFKDIGSYPSLNKQTSPLTSWNPGSNYSYRV
QTPEYTKEFVTKYAGSQSTTLVFGY
>Mature_345_residues
MKRLCLWFTVFSLFLVLLPGKALGAVDFPNTSTNGLLGFAGNAKNEKGISKTGTTGGKNGQIVYIQSVNDLKTHLGGSTP
KILVLQNDLSASAKTTVTIGSNKTLVGSYAKKTLKNIYLTTSSAFGNVIFQNLTFEHSPQINGNNDIQLYLDSGMNYWID
HVTFSGHSYNASGSDLDKLVYIGKSADYITISNSKFANHKYGLILGYPDDSQHQYDGYPHMTIANNYFENLYVRGPGLMR
YGYFHVKNNYSNNFNQAITIATKAKIYSEYNYFGKGSEKGGILDDKGTGYFKDIGSYPSLNKQTSPLTSWNPGSNYSYRV
QTPEYTKEFVTKYAGSQSTTLVFGY

Specific function: Catalyzes the depolymerization of pectins of methyl esterification degree from 13 to 75%, with an endo mode of action. Can not degrade polygalacturonate

COG id: COG3866

COG function: function code G; Pectate lyase

Gene ontology:

Cell location: Secreted

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide lyase 1 family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PEL1_BACSU (O34819)

Other databases:

- EMBL:   AF027868
- EMBL:   AL009126
- PIR:   E69674
- RefSeq:   NP_389746.1
- HSSP:   P39116
- ProteinModelPortal:   O34819
- SMR:   O34819
- EnsemblBacteria:   EBBACT00000002973
- GeneID:   940111
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU18650
- NMPDR:   fig|224308.1.peg.1868
- GenoList:   BSU18650
- GeneTree:   EBGT00050000000877
- HOGENOM:   HBG338481
- OMA:   LMRYGYF
- ProtClustDB:   CLSK887387
- BioCyc:   BSUB:BSU18650-MONOMER
- InterPro:   IPR002022
- InterPro:   IPR012334
- InterPro:   IPR011050
- Gene3D:   G3DSA:2.160.20.10
- SMART:   SM00656

Pfam domain/function: PF00544 Pec_lyase_C; SSF51126 Pectin_lyas_like

EC number: =4.2.2.10

Molecular weight: Translated: 38108; Mature: 38108

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: ACT_SITE 234-234

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLCLWFTVFSLFLVLLPGKALGAVDFPNTSTNGLLGFAGNAKNEKGISKTGTTGGKNG
CCEEEHHHHHHHHHHHHCCCCHHCEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCC
QIVYIQSVNDLKTHLGGSTPKILVLQNDLSASAKTTVTIGSNKTLVGSYAKKTLKNIYLT
CEEEEECCCHHHHHHCCCCCEEEEEECCCCCCCEEEEEECCCCEEEHHHHHHHHHEEEEE
TSSAFGNVIFQNLTFEHSPQINGNNDIQLYLDSGMNYWIDHVTFSGHSYNASGSDLDKLV
ECCHHCCEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCCCCEEEE
YIGKSADYITISNSKFANHKYGLILGYPDDSQHQYDGYPHMTIANNYFENLYVRGPGLMR
EECCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEECCCCCEEEEECCCEEE
YGYFHVKNNYSNNFNQAITIATKAKIYSEYNYFGKGSEKGGILDDKGTGYFKDIGSYPSL
EEEEEEECCCCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCEECCCCCCHHHHCCCCCCC
NKQTSPLTSWNPGSNYSYRVQTPEYTKEFVTKYAGSQSTTLVFGY
CCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCEEEEEC
>Mature Secondary Structure
MKRLCLWFTVFSLFLVLLPGKALGAVDFPNTSTNGLLGFAGNAKNEKGISKTGTTGGKNG
CCEEEHHHHHHHHHHHHCCCCHHCEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCC
QIVYIQSVNDLKTHLGGSTPKILVLQNDLSASAKTTVTIGSNKTLVGSYAKKTLKNIYLT
CEEEEECCCHHHHHHCCCCCEEEEEECCCCCCCEEEEEECCCCEEEHHHHHHHHHEEEEE
TSSAFGNVIFQNLTFEHSPQINGNNDIQLYLDSGMNYWIDHVTFSGHSYNASGSDLDKLV
ECCHHCCEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCCCCEEEE
YIGKSADYITISNSKFANHKYGLILGYPDDSQHQYDGYPHMTIANNYFENLYVRGPGLMR
EECCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEECCCCCEEEEECCCEEE
YGYFHVKNNYSNNFNQAITIATKAKIYSEYNYFGKGSEKGGILDDKGTGYFKDIGSYPSL
EEEEEEECCCCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCEECCCCCCHHHHCCCCCCC
NKQTSPLTSWNPGSNYSYRVQTPEYTKEFVTKYAGSQSTTLVFGY
CCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377