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The map label for this gene is yoaI

Identifier: 255767439

GI number: 255767439

Start: 2031439

End: 2032890

Strand: Direct

Name: yoaI

Synonym: BSU18620

Alternate gene names: 255767439

Gene position: 2031439-2032890 (Clockwise)

Preceding gene: 50812245

Following gene: 16078925

Centisome position: 48.19

GC content: 37.53

Gene sequence:

>1452_bases
ATGGGGATCATCAATGGTAAAGAATTCATTGACCGATTGAATAAACTAGAAAACGAAATATGGTATGATGGCGAAAAAAT
AAAGGGTAACATTTCTGAGCATCCTGCATTTAAAGGAATAATCAAAACAAAAAGCTCACTTTATGAATTGCAAACAAAGG
ATGAGTTAATACATGAAATGACTTATTGCCTTCCTGGAGATCATAATCGAATCGGCCTTTCCTATCTCCAGCCTAAAACA
AAAAATGATTTGAAAAAAAGAAGGACCATGATTGAACATTGGGCTAGACATACTCATGGAATGATGGGGAGAAGTCCGGA
TTACATGAATACGGTTATGATGAGCTTTGCTTCGTCAGCGGAGCTTCTAAAAGATAAAGAGAATTGCTTTCCTGAACATA
TACTAGATATGTATGAACAAGCTGCTAAGCATGACCTTTCCTTTACCCATACATTCATTACTCCGCAAGTGAACCGGTCA
CAGTCCTATTTTGGGCTAAGTGAAAAACCGATATCTGCTAAAGTCATTGATAGAACCGAAAAAGGTTTAATGATTCATGG
CGCACGACTGCTTGCCACACAAGGCGGGTTGACCGATGAAATCTTGGTTTTTTCAGCGCCTAAATTTTTCTTTGAAACAG
ATGAAGCTTATGCTTTTTCAATCCCTTCTAATACAAAAGGCGTAAAATTCATCACAAGAGAATCATTTGTATTAAGCGAC
TCTTCCTTTAATCATCCCTTAAGCTCAAGGTATGAAGAAATGGATTCAATTGTGGTTTTTGATCATGTGTTAGTGCCGTG
GAATCGTGTGTTTTTTTACGATAATGTGGAAGCAGCTAAAGATTTTATGACAAAAAGCTCTTTTCATGCCTTTACCTTTC
ACCAGGTTGTCATTAGGCAAATGATAAAAATTGAATTTCTATTAGGTGTTGCACAGCTTCTTGTCGACACCATTAATGTT
TCCGAATATCAGCACATCCAAGAAAAGCTCTCAGAAATCATTGTGGGATTGGAAACAATCAAAGCCCTCATTGACAAATC
GGAAAATGATGCTCAGTTAGATGAATTCGGCTATATGCGCCCCTGTTTAATTCCTCTTCAAGTCATCAGTACGATCATTC
CTAAGCTATATCCTCGATTTACTGAAATCATACAATTAATTGGAGCTAGCGGAATGGTGACACTGCCTACCGAAAATGCA
TTTGACTCAGAGATACGGGAAGATCTTGACCAATATCTTCAAGCAACCAATACAAATGCTGAAGAACGTGTGAAAATTTT
CCGTTTGGCATGGGATTTAACAATGAGTTCATTCGGAACTAGACAAACTCACTACGAAAGATATTTTTTCGGAGATCCTA
TTCGGATTTCAAGCAGGCTGTATACCAGTTATCCAAAGCAGGAGCAGTTGAACATGATTAAAACATTTTTACATGCAGAT
ACAGAACATTGA

Upstream 100 bases:

>100_bases
TTTTCTAAAAAAGGCTTTTCCCACCTCAAATTGGCTGAACCGGCTGTTTTCTCGAAAATATAATACAATACGCAGCATAA
TATGAAGGAGCGAAACTTAA

Downstream 100 bases:

>100_bases
AAAAACAGCGGGAAAAGATCCGCTGTTTCGTATTTTTTATTCAGGAAACTGAACATGGCCCGGTACTGTATAGGCTTTGG
ACGTTCCGCTTTCAGGCAGC

Product: 4-hydroxyphenylacetate-3-hydroxylase

Products: NA

Alternate protein names: Putative 4-HPA 3-hydroxylase; Putative 4-hydroxyphenylacetate-3-hydroxylase

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MGIINGKEFIDRLNKLENEIWYDGEKIKGNISEHPAFKGIIKTKSSLYELQTKDELIHEMTYCLPGDHNRIGLSYLQPKT
KNDLKKRRTMIEHWARHTHGMMGRSPDYMNTVMMSFASSAELLKDKENCFPEHILDMYEQAAKHDLSFTHTFITPQVNRS
QSYFGLSEKPISAKVIDRTEKGLMIHGARLLATQGGLTDEILVFSAPKFFFETDEAYAFSIPSNTKGVKFITRESFVLSD
SSFNHPLSSRYEEMDSIVVFDHVLVPWNRVFFYDNVEAAKDFMTKSSFHAFTFHQVVIRQMIKIEFLLGVAQLLVDTINV
SEYQHIQEKLSEIIVGLETIKALIDKSENDAQLDEFGYMRPCLIPLQVISTIIPKLYPRFTEIIQLIGASGMVTLPTENA
FDSEIREDLDQYLQATNTNAEERVKIFRLAWDLTMSSFGTRQTHYERYFFGDPIRISSRLYTSYPKQEQLNMIKTFLHAD
TEH

Sequences:

>Translated_483_residues
MGIINGKEFIDRLNKLENEIWYDGEKIKGNISEHPAFKGIIKTKSSLYELQTKDELIHEMTYCLPGDHNRIGLSYLQPKT
KNDLKKRRTMIEHWARHTHGMMGRSPDYMNTVMMSFASSAELLKDKENCFPEHILDMYEQAAKHDLSFTHTFITPQVNRS
QSYFGLSEKPISAKVIDRTEKGLMIHGARLLATQGGLTDEILVFSAPKFFFETDEAYAFSIPSNTKGVKFITRESFVLSD
SSFNHPLSSRYEEMDSIVVFDHVLVPWNRVFFYDNVEAAKDFMTKSSFHAFTFHQVVIRQMIKIEFLLGVAQLLVDTINV
SEYQHIQEKLSEIIVGLETIKALIDKSENDAQLDEFGYMRPCLIPLQVISTIIPKLYPRFTEIIQLIGASGMVTLPTENA
FDSEIREDLDQYLQATNTNAEERVKIFRLAWDLTMSSFGTRQTHYERYFFGDPIRISSRLYTSYPKQEQLNMIKTFLHAD
TEH
>Mature_482_residues
GIINGKEFIDRLNKLENEIWYDGEKIKGNISEHPAFKGIIKTKSSLYELQTKDELIHEMTYCLPGDHNRIGLSYLQPKTK
NDLKKRRTMIEHWARHTHGMMGRSPDYMNTVMMSFASSAELLKDKENCFPEHILDMYEQAAKHDLSFTHTFITPQVNRSQ
SYFGLSEKPISAKVIDRTEKGLMIHGARLLATQGGLTDEILVFSAPKFFFETDEAYAFSIPSNTKGVKFITRESFVLSDS
SFNHPLSSRYEEMDSIVVFDHVLVPWNRVFFYDNVEAAKDFMTKSSFHAFTFHQVVIRQMIKIEFLLGVAQLLVDTINVS
EYQHIQEKLSEIIVGLETIKALIDKSENDAQLDEFGYMRPCLIPLQVISTIIPKLYPRFTEIIQLIGASGMVTLPTENAF
DSEIREDLDQYLQATNTNAEERVKIFRLAWDLTMSSFGTRQTHYERYFFGDPIRISSRLYTSYPKQEQLNMIKTFLHADT
EH

Specific function: Catalyzs the hydroxylation of 4-hydroxyphenylacetic acid (4HPA), leading to the production of 3,4-dihydroxyphenylacetic acid (DHPA)

COG id: COG2368

COG function: function code Q; Aromatic ring hydroxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FADH(2)-utilizing monooxygenase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YOAI_BACSU (C0SPC0)

Other databases:

- EMBL:   AF027868
- EMBL:   AL009126
- PIR:   C69896
- RefSeq:   NP_389743.2
- ProteinModelPortal:   C0SPC0
- SMR:   C0SPC0
- EnsemblBacteria:   EBBACT00000001977
- GeneID:   940107
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU18620
- NMPDR:   fig|224308.1.peg.1865
- GenoList:   BSU18620
- GeneTree:   EBGT00050000002318
- ProtClustDB:   CLSK887385
- BioCyc:   BSUB:BSU18620-MONOMER
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR009100
- InterPro:   IPR004925
- InterPro:   IPR012687
- Gene3D:   G3DSA:2.40.110.10
- Gene3D:   G3DSA:1.20.140.10
- PIRSF:   PIRSF000331
- TIGRFAMs:   TIGR02309

Pfam domain/function: PF03241 HpaB; SSF56645 AcylCoA_dehyd_NM; SSF47203 AcylCoADH_C_like

EC number: NA

Molecular weight: Translated: 55851; Mature: 55719

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: BINDING 150-150 BINDING 193-193

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIINGKEFIDRLNKLENEIWYDGEKIKGNISEHPAFKGIIKTKSSLYELQTKDELIHEM
CCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TYCLPGDHNRIGLSYLQPKTKNDLKKRRTMIEHWARHTHGMMGRSPDYMNTVMMSFASSA
HHHCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
ELLKDKENCFPEHILDMYEQAAKHDLSFTHTFITPQVNRSQSYFGLSEKPISAKVIDRTE
HHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCC
KGLMIHGARLLATQGGLTDEILVFSAPKFFFETDEAYAFSIPSNTKGVKFITRESFVLSD
CCEEEECCEEEEECCCCCCCEEEEECCHHEEECCCCEEEECCCCCCCEEEEEECCEEECC
SSFNHPLSSRYEEMDSIVVFDHVLVPWNRVFFYDNVEAAKDFMTKSSFHAFTFHQVVIRQ
CCCCCCHHHHHHHHCCEEEEHHHHCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHH
MIKIEFLLGVAQLLVDTINVSEYQHIQEKLSEIIVGLETIKALIDKSENDAQLDEFGYMR
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCC
PCLIPLQVISTIIPKLYPRFTEIIQLIGASGMVTLPTENAFDSEIREDLDQYLQATNTNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCH
EERVKIFRLAWDLTMSSFGTRQTHYERYFFGDPIRISSRLYTSYPKQEQLNMIKTFLHAD
HHHHHHHHHHHHHHHHHCCCCHHHHHHEECCCCEEECHHHHHCCCCHHHHHHHHHHHHCC
TEH
CCC
>Mature Secondary Structure 
GIINGKEFIDRLNKLENEIWYDGEKIKGNISEHPAFKGIIKTKSSLYELQTKDELIHEM
CCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TYCLPGDHNRIGLSYLQPKTKNDLKKRRTMIEHWARHTHGMMGRSPDYMNTVMMSFASSA
HHHCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
ELLKDKENCFPEHILDMYEQAAKHDLSFTHTFITPQVNRSQSYFGLSEKPISAKVIDRTE
HHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCC
KGLMIHGARLLATQGGLTDEILVFSAPKFFFETDEAYAFSIPSNTKGVKFITRESFVLSD
CCEEEECCEEEEECCCCCCCEEEEECCHHEEECCCCEEEECCCCCCCEEEEEECCEEECC
SSFNHPLSSRYEEMDSIVVFDHVLVPWNRVFFYDNVEAAKDFMTKSSFHAFTFHQVVIRQ
CCCCCCHHHHHHHHCCEEEEHHHHCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHH
MIKIEFLLGVAQLLVDTINVSEYQHIQEKLSEIIVGLETIKALIDKSENDAQLDEFGYMR
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCC
PCLIPLQVISTIIPKLYPRFTEIIQLIGASGMVTLPTENAFDSEIREDLDQYLQATNTNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCH
EERVKIFRLAWDLTMSSFGTRQTHYERYFFGDPIRISSRLYTSYPKQEQLNMIKTFLHAD
HHHHHHHHHHHHHHHHHCCCCHHHHHHEECCCCEEECHHHHHCCCCHHHHHHHHHHHHCC
TEH
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377