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The map label for this gene is proJ

Identifier: 16078908

GI number: 16078908

Start: 2015733

End: 2016848

Strand: Reverse

Name: proJ

Synonym: BSU18470

Alternate gene names: 16078908

Gene position: 2016848-2015733 (Counterclockwise)

Preceding gene: 50812244

Following gene: 255767434

Centisome position: 47.84

GC content: 44.0

Gene sequence:

>1116_bases
GTGACGCCTGATACAAGCATGAAAAGAGTCGTTGTGAAGATTGGAAGCAGTTCATTAACAAGCCTTCATGGAGAAATCAG
CATTCGAAAATTAGAAGCGTTGGTCGACCAGGTCGTCAAACTCAAAGACGCCGGTTATGAAGTGATTTTGGTTTCCTCAG
GCGCAGTGGCGGCGGGATATCGGAAGCTCGGATTTATCCAAAGGCCCGAGAAGCTTCCAGAAAAACAAGCATCAGCTTCA
ATCGGCCAGGGATTGCTAATGGAGGCTTATTCAAAGCTGTTTTTGGCACACGGCTATGTCGCATCCCAAATTTTAATTAC
GAGAAGCGATTTTTCTGATGAATATCGATACAACAATGTCAGGAATACAATGAATGTACTGCTCGAGCGCGGCATCATCC
CGATCATAAACGAAAATGACACAGTTACCGTCAATCGGCTCAAGTTTGGCGACAATGATACGCTCGCAGCAAAAGTCGCA
GGATTGATTGATGCAGATATGCTTGTGATTTTATCAGATATAGACGGATTATATGACGGCAATCCGCGCACAAATCCAGA
AGCGAAAAAGATACAGCGGGTGAGTGAAATAACACCTGACATCGAAGCATGCGCAGGTGACACTGGAAGCATCGTTGGCA
CCGGCGGCATGCGCTCTAAGCTTGATGCGTTTAAAATCGTAATGGCGTCTGGCATTAAAGGTTTCCTAGGTCAGGCAGAT
GCTGGCGATATTTTATACCACGCCGTTCATGAACAGGCGGAAGGCACCTATTTTGAAGCAGAAGGAACGCTTCCGTTGAA
TCAAAAGGAGCAATGGATCGCTTTTAATTCCGGTCCGGAAGGTGAAATGATTTTATCAGATGATTGTTCACGAAAAATAA
CAAACGGACAATCGAGTTTATACCTAGACGGTGTACAGAAGATTAAAGGGAAATTCAAAAGCGGTTCAGTTGTTCGGCTG
ATGGATTCAAAAGGGACGGAAATCGGCCTTGGAATCGTCAATTACTCTTCTGTACAGCTTCAAGAACCGGAAAAGAAAAA
GGAACTGACAAATAGAGCTCTAATTGATCAAGAAGCATTTGTTTGCCATGTGGATTTTTCTTTACCTGTAAATTAA

Upstream 100 bases:

>100_bases
GAGAAGCGATTTCTCAAGCAATAAAGCATGCGGCTAAGCGTTCAAAGGAAATCAGCGAAGATATCGAAAAAACTGCAGCG
CCGCTATCAGGAGTGATAAA

Downstream 100 bases:

>100_bases
TTTGCAGAAAAAAACCCGAAGCTTCTATATAGAAGCTCGGGTTCATTTTTTTTATTGATACTGCTCCAGCTTAGAGAAAA
ATTGAATGACAAACTCATAA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase 2; GK 2

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MTPDTSMKRVVVKIGSSSLTSLHGEISIRKLEALVDQVVKLKDAGYEVILVSSGAVAAGYRKLGFIQRPEKLPEKQASAS
IGQGLLMEAYSKLFLAHGYVASQILITRSDFSDEYRYNNVRNTMNVLLERGIIPIINENDTVTVNRLKFGDNDTLAAKVA
GLIDADMLVILSDIDGLYDGNPRTNPEAKKIQRVSEITPDIEACAGDTGSIVGTGGMRSKLDAFKIVMASGIKGFLGQAD
AGDILYHAVHEQAEGTYFEAEGTLPLNQKEQWIAFNSGPEGEMILSDDCSRKITNGQSSLYLDGVQKIKGKFKSGSVVRL
MDSKGTEIGLGIVNYSSVQLQEPEKKKELTNRALIDQEAFVCHVDFSLPVN

Sequences:

>Translated_371_residues
MTPDTSMKRVVVKIGSSSLTSLHGEISIRKLEALVDQVVKLKDAGYEVILVSSGAVAAGYRKLGFIQRPEKLPEKQASAS
IGQGLLMEAYSKLFLAHGYVASQILITRSDFSDEYRYNNVRNTMNVLLERGIIPIINENDTVTVNRLKFGDNDTLAAKVA
GLIDADMLVILSDIDGLYDGNPRTNPEAKKIQRVSEITPDIEACAGDTGSIVGTGGMRSKLDAFKIVMASGIKGFLGQAD
AGDILYHAVHEQAEGTYFEAEGTLPLNQKEQWIAFNSGPEGEMILSDDCSRKITNGQSSLYLDGVQKIKGKFKSGSVVRL
MDSKGTEIGLGIVNYSSVQLQEPEKKKELTNRALIDQEAFVCHVDFSLPVN
>Mature_370_residues
TPDTSMKRVVVKIGSSSLTSLHGEISIRKLEALVDQVVKLKDAGYEVILVSSGAVAAGYRKLGFIQRPEKLPEKQASASI
GQGLLMEAYSKLFLAHGYVASQILITRSDFSDEYRYNNVRNTMNVLLERGIIPIINENDTVTVNRLKFGDNDTLAAKVAG
LIDADMLVILSDIDGLYDGNPRTNPEAKKIQRVSEITPDIEACAGDTGSIVGTGGMRSKLDAFKIVMASGIKGFLGQADA
GDILYHAVHEQAEGTYFEAEGTLPLNQKEQWIAFNSGPEGEMILSDDCSRKITNGQSSLYLDGVQKIKGKFKSGSVVRLM
DSKGTEIGLGIVNYSSVQLQEPEKKKELTNRALIDQEAFVCHVDFSLPVN

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=288, Percent_Identity=32.9861111111111, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI62912457, Length=286, Percent_Identity=32.5174825174825, Blast_Score=122, Evalue=8e-28,
Organism=Escherichia coli, GI1786437, Length=333, Percent_Identity=36.3363363363363, Blast_Score=223, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17569967, Length=267, Percent_Identity=32.5842696629214, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17569969, Length=267, Percent_Identity=32.5842696629214, Blast_Score=129, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6321822, Length=242, Percent_Identity=44.2148760330579, Blast_Score=196, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6320506, Length=235, Percent_Identity=44.2553191489362, Blast_Score=190, Evalue=3e-49,
Organism=Drosophila melanogaster, GI21357643, Length=258, Percent_Identity=32.9457364341085, Blast_Score=118, Evalue=7e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROJ_BACSU (O07509)

Other databases:

- EMBL:   AF006720
- EMBL:   AL009126
- PIR:   F69682
- RefSeq:   NP_389729.1
- ProteinModelPortal:   O07509
- SMR:   O07509
- EnsemblBacteria:   EBBACT00000002394
- GeneID:   940098
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU18470
- NMPDR:   fig|224308.1.peg.1851
- GenoList:   BSU18470
- GeneTree:   EBGT00050000001887
- HOGENOM:   HBG507643
- OMA:   NVRNTMN
- PhylomeDB:   O07509
- ProtClustDB:   PRK13402
- BioCyc:   BSUB:BSU18470-MONOMER
- BRENDA:   2.7.2.11
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 40355; Mature: 40223

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPDTSMKRVVVKIGSSSLTSLHGEISIRKLEALVDQVVKLKDAGYEVILVSSGAVAAGY
CCCCCCHHEEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHH
RKLGFIQRPEKLPEKQASASIGQGLLMEAYSKLFLAHGYVASQILITRSDFSDEYRYNNV
HHHCCCCCCHHCCHHHHHHHHCCHHHHHHHHHHHHHCCHHHEEEEEEECCCCCHHHHHHH
RNTMNVLLERGIIPIINENDTVTVNRLKFGDNDTLAAKVAGLIDADMLVILSDIDGLYDG
HHHHHHHHHCCCEEEECCCCEEEEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCCC
NPRTNPEAKKIQRVSEITPDIEACAGDTGSIVGTGGMRSKLDAFKIVMASGIKGFLGQAD
CCCCCCHHHHHHHHHHCCCCHHHHCCCCCCEEECCCCHHHHHHHHHHHHHCCHHHCCCCC
AGDILYHAVHEQAEGTYFEAEGTLPLNQKEQWIAFNSGPEGEMILSDDCSRKITNGQSSL
CCHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEECCCCCCCEEECCCCCHHHCCCCCEE
YLDGVQKIKGKFKSGSVVRLMDSKGTEIGLGIVNYSSVQLQEPEKKKELTNRALIDQEAF
EHHHHHHHHCCCCCCCEEEEECCCCCEEEEEEEECCCEEECCCHHHHHHHHHHHCCCCEE
VCHVDFSLPVN
EEEEEEECCCC
>Mature Secondary Structure 
TPDTSMKRVVVKIGSSSLTSLHGEISIRKLEALVDQVVKLKDAGYEVILVSSGAVAAGY
CCCCCHHEEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHH
RKLGFIQRPEKLPEKQASASIGQGLLMEAYSKLFLAHGYVASQILITRSDFSDEYRYNNV
HHHCCCCCCHHCCHHHHHHHHCCHHHHHHHHHHHHHCCHHHEEEEEEECCCCCHHHHHHH
RNTMNVLLERGIIPIINENDTVTVNRLKFGDNDTLAAKVAGLIDADMLVILSDIDGLYDG
HHHHHHHHHCCCEEEECCCCEEEEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCCC
NPRTNPEAKKIQRVSEITPDIEACAGDTGSIVGTGGMRSKLDAFKIVMASGIKGFLGQAD
CCCCCCHHHHHHHHHHCCCCHHHHCCCCCCEEECCCCHHHHHHHHHHHHHCCHHHCCCCC
AGDILYHAVHEQAEGTYFEAEGTLPLNQKEQWIAFNSGPEGEMILSDDCSRKITNGQSSL
CCHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEECCCCCCCEEECCCCCHHHCCCCCEE
YLDGVQKIKGKFKSGSVVRLMDSKGTEIGLGIVNYSSVQLQEPEKKKELTNRALIDQEAF
EHHHHHHHHCCCCCCCEEEEECCCCCEEEEEEEECCCEEECCCHHHHHHHHHHHCCCCEE
VCHVDFSLPVN
EEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377