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The map label for this gene is proH

Identifier: 50812244

GI number: 50812244

Start: 2016845

End: 2017738

Strand: Reverse

Name: proH

Synonym: BSU18480

Alternate gene names: 50812244

Gene position: 2017738-2016845 (Counterclockwise)

Preceding gene: 16078910

Following gene: 16078908

Centisome position: 47.86

GC content: 44.74

Gene sequence:

>894_bases
ATGCGAACAAAAAAGCGAACAAAGGAGATGTTACCGATCTTTGATCAAAAGAAAGTAGCCTTTATAGGAGCAGGATCTAT
GGCGGAAGGAATGATATCAGGTATCGTTCGAGCCAACAAAATCCCGAAACAAAACATCTGCGTTACAAACCGCAGCAATA
CAGAGCGATTAACTGAACTTGAACTTCAATATGGCATTAAAGGCGCCTTGCCGAACCAACTATGTATTGAAGACATGGAT
GTGCTCATTTTGGCAATGAAGCCAAAAGACGCCGAAAACGCTCTGTCATCACTAAAATCACGTATTCAACCCCATCAATT
AATATTGTCGGTCCTTGCCGGCATAACTACCTCTTTTATTGAACAATCATTGCTTAATGAACAGCCGGTTGTCAGAGTGA
TGCCCAACACTTCCAGTATGATTGGCGCTTCTGCAACAGCTATTGCCCTTGGCAAATATGTGTCGGAAGACCTGAAGAAG
CTGGCCGAAGCATTGCTCGGATGCATGGGCGAAGTCTATACAATCCAAGAAAATCAAATGGATATATTCACCGGAATTGC
AGGCAGCGGACCCGCGTATTTTTATTATTTAATGGAATTCATTGAAAAGACAGGCGAGGAAGCGGGCCTCGATAAACAGT
TATCTCGAAGCATCGGCGCACAGACACTTTTAGGTGCCGCAAAAATGCTCATGGAAACAGGAGAACATCCTGAGATCTTA
AGAGATAATATCACGTCACCGAACGGAACGACAGCAGCGGGGCTGCAAGCCTTGAAAAAGAGCGGCGGCGGAGAAGCGAT
TTCTCAAGCAATAAAGCATGCGGCTAAGCGTTCAAAGGAAATCAGCGAAGATATCGAAAAAACTGCAGCGCCGCTATCAG
GAGTGATAAAGTGA

Upstream 100 bases:

>100_bases
TTTCATTATACATATTTGTCGAAAAGAAACAATACAAATCAATAATGGCCTTCAAACTTGACATCATTTCCTCACGTGGT
AACATTTTAAACGTGTGAAA

Downstream 100 bases:

>100_bases
CGCCTGATACAAGCATGAAAAGAGTCGTTGTGAAGATTGGAAGCAGTTCATTAACAAGCCTTCATGGAGAAATCAGCATT
CGAAAATTAGAAGCGTTGGT

Product: pyrroline-5-carboxylate reductase

Products: NA

Alternate protein names: P5C reductase 1; P5CR 1 [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MRTKKRTKEMLPIFDQKKVAFIGAGSMAEGMISGIVRANKIPKQNICVTNRSNTERLTELELQYGIKGALPNQLCIEDMD
VLILAMKPKDAENALSSLKSRIQPHQLILSVLAGITTSFIEQSLLNEQPVVRVMPNTSSMIGASATAIALGKYVSEDLKK
LAEALLGCMGEVYTIQENQMDIFTGIAGSGPAYFYYLMEFIEKTGEEAGLDKQLSRSIGAQTLLGAAKMLMETGEHPEIL
RDNITSPNGTTAAGLQALKKSGGGEAISQAIKHAAKRSKEISEDIEKTAAPLSGVIK

Sequences:

>Translated_297_residues
MRTKKRTKEMLPIFDQKKVAFIGAGSMAEGMISGIVRANKIPKQNICVTNRSNTERLTELELQYGIKGALPNQLCIEDMD
VLILAMKPKDAENALSSLKSRIQPHQLILSVLAGITTSFIEQSLLNEQPVVRVMPNTSSMIGASATAIALGKYVSEDLKK
LAEALLGCMGEVYTIQENQMDIFTGIAGSGPAYFYYLMEFIEKTGEEAGLDKQLSRSIGAQTLLGAAKMLMETGEHPEIL
RDNITSPNGTTAAGLQALKKSGGGEAISQAIKHAAKRSKEISEDIEKTAAPLSGVIK
>Mature_297_residues
MRTKKRTKEMLPIFDQKKVAFIGAGSMAEGMISGIVRANKIPKQNICVTNRSNTERLTELELQYGIKGALPNQLCIEDMD
VLILAMKPKDAENALSSLKSRIQPHQLILSVLAGITTSFIEQSLLNEQPVVRVMPNTSSMIGASATAIALGKYVSEDLKK
LAEALLGCMGEVYTIQENQMDIFTGIAGSGPAYFYYLMEFIEKTGEEAGLDKQLSRSIGAQTLLGAAKMLMETGEHPEIL
RDNITSPNGTTAAGLQALKKSGGGEAISQAIKHAAKRSKEISEDIEKTAAPLSGVIK

Specific function: Proline biosynthesis; third (last) step. [C]

COG id: COG0345

COG function: function code E; Pyrroline-5-carboxylate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrroline-5-carboxylate reductase family [H]

Homologues:

Organism=Homo sapiens, GI198041662, Length=267, Percent_Identity=33.3333333333333, Blast_Score=148, Evalue=6e-36,
Organism=Homo sapiens, GI24797097, Length=266, Percent_Identity=31.9548872180451, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI21361454, Length=267, Percent_Identity=31.8352059925094, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI24797095, Length=266, Percent_Identity=31.9548872180451, Blast_Score=142, Evalue=4e-34,
Organism=Escherichia coli, GI1786585, Length=268, Percent_Identity=28.3582089552239, Blast_Score=142, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17569021, Length=262, Percent_Identity=33.587786259542, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17540664, Length=284, Percent_Identity=29.5774647887324, Blast_Score=114, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6320861, Length=283, Percent_Identity=28.9752650176678, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21358587, Length=269, Percent_Identity=32.3420074349442, Blast_Score=151, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24648116, Length=279, Percent_Identity=31.8996415770609, Blast_Score=141, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24647700, Length=169, Percent_Identity=35.5029585798817, Blast_Score=119, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.5.1.2 [H]

Molecular weight: Translated: 32032; Mature: 32032

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: PS00521 P5CR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTKKRTKEMLPIFDQKKVAFIGAGSMAEGMISGIVRANKIPKQNICVTNRSNTERLTEL
CCCHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHH
ELQYGIKGALPNQLCIEDMDVLILAMKPKDAENALSSLKSRIQPHQLILSVLAGITTSFI
HHHHCCCCCCCCHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
EQSLLNEQPVVRVMPNTSSMIGASATAIALGKYVSEDLKKLAEALLGCMGEVYTIQENQM
HHHHCCCCCEEEECCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCH
DIFTGIAGSGPAYFYYLMEFIEKTGEEAGLDKQLSRSIGAQTLLGAAKMLMETGEHPEIL
HHHHCCCCCCHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
RDNITSPNGTTAAGLQALKKSGGGEAISQAIKHAAKRSKEISEDIEKTAAPLSGVIK
HHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRTKKRTKEMLPIFDQKKVAFIGAGSMAEGMISGIVRANKIPKQNICVTNRSNTERLTEL
CCCHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHH
ELQYGIKGALPNQLCIEDMDVLILAMKPKDAENALSSLKSRIQPHQLILSVLAGITTSFI
HHHHCCCCCCCCHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
EQSLLNEQPVVRVMPNTSSMIGASATAIALGKYVSEDLKKLAEALLGCMGEVYTIQENQM
HHHHCCCCCEEEECCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCH
DIFTGIAGSGPAYFYYLMEFIEKTGEEAGLDKQLSRSIGAQTLLGAAKMLMETGEHPEIL
HHHHCCCCCCHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
RDNITSPNGTTAAGLQALKKSGGGEAISQAIKHAAKRSKEISEDIEKTAAPLSGVIK
HHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1849493; 2493444 [H]