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The map label for this gene is nrdE

Identifier: 16078801

GI number: 16078801

Start: 1868969

End: 1871071

Strand: Direct

Name: nrdE

Synonym: BSU17380

Alternate gene names: 16078801

Gene position: 1868969-1871071 (Clockwise)

Preceding gene: 16078800

Following gene: 16078802

Centisome position: 44.33

GC content: 41.99

Gene sequence:

>2103_bases
TTGTCACAAAATCAAGTGCCAAAATGGATCCAGTTAAATAACGAAATCATGATCCAAAAAGATGGGAAATTCCAGTTTGA
TAAGGATAAAGAAGCTGTACATAGCTATTTTGTAGATTATATCAATCAAAACACAGTCTTCTTTCACAATTTAAAAGAGA
AGCTGGATTATTTGGTTGAAAACCAATACTACGAAGAGGAATTCTTAAGCCTTTATTCTTTTGAAGACATTAAAGAAGTG
TTTAAGACAGCTTACGCTAAGAAGTTTCGTTTTCCTTCCTTCATGAGTGCGTTTAAATTCTATAATGACTATGCTTTGAA
AACGAATGACAAGAAAAAAATCCTCGAGCGTTATGAGGACCGGATCTCAATTGTTGCGCTGTTCTTCGCTAACGGCGACA
CTGAGAAAGCAAAAGAATATGTAAACCTGATGATCAATCAAGAATATCAGCCGAGCACACCGACATTTTTGAATGCCGGC
AGAAAACGCCGCGGTGAACTTGTGAGCTGCTTCTTGCTCGAAGTTAATGATTCTTTAAATGACATTTCAAGAGCAATCGA
TATCTCCATGCAGCTTTCTAAGCTTGGTGGCGGGGTTTCTCTTAACCTGTCCAAGCTTCGCGCGAAAGGTGAAGCCATTA
AAGATGTTGAGAATGCGACAAAAGGCGTTGTAGGCGTCATGAAGCTTCTTGATAACGCGTTCCGTTACGCTGACCAAATG
GGACAAAGACAAGGCTCCGGCGCGGCGTATTTAAACATTTTCCACCGCGATATCAATGATTTTCTTGATACGAAAAAGAT
TTCTGCGGATGAAGATGTGCGTGTCAAAACGCTTTCGATCGGTGTTGTCATTCCGGATAAGTTCGTTGAGCTTGCACGCG
AGGACAAAGCGGCTTATGTATTCTACCCGCACACGATTTATAAAGAGTACGGCCAGCACATGGATGAGATGGACATGAAC
GAAATGTACGACAAGTTTGTCGACAACCCTCGGGTGAAAAAGGAAAAAATCAACCCGCGGAAACTGCTTGAAAAATTGGC
GATGCTTCGTTCTGAATCAGGCTATCCATACATCATGTTCCAAGATAACGTCAATAAAGTGCATGCGAATAACCATATTT
CCAAGGTCAAATTTTCTAACCTTTGCTCTGAAGTGCTGCAGGCATCTCAAGTTTCTTCATACACAGACTATGATGAAGAA
GATGAAATCGGTCTGGATATTTCCTGCAACCTGGGTTCGCTTAACATTCTCAATGTCATGGAGCATAAATCAATCGAAAA
GACAGTCAAGCTTGCGACAGACTCTTTAACACACGTGTCTGAAACAACAGACATCCGTAATGCGCCTGCTGTAAGACGGG
CAAACAAAGCGATGAAATCAATCGGCCTCGGTGCTATGAACCTTCACGGCTACCTTGCGCAAAACGGCATTGCGTATGAA
AGTCCGGAAGCGCGTGACTTTGCAAACACGTTCTTTATGATGGTGAATTTCTACTCCATCCAGCGTTCTGCGGAAATCGC
AAAAGAGAAGGGCGAAACGTTTGATCAATATGAAGGATCAACCTATGCGACAGGTGAATACTTCGACAAATACGTTTCAA
CTGATTTCTCACCGAAATACGAAAAAATCGCTAATCTGTTTGAAGGTATGCATATCCCGACGACAGAGGACTGGAAAAAG
CTAAAAGCATTTGTCGCTGAACACGGTATGTATCACAGCTACAGATTATGCATTGCGCCGACAGGCTCTATCTCATACGT
TCAATCAAGCACGGCGTCTGTTATGCCGATTATGGAACGGATTGAAGAACGAACATACGGCAACTCAAAAACGTACTACC
CAATGCCTGGGCTTGCATCTAATAACTGGTTCTTCTATAAAGAAGCCTACGATATGGACATGTTCAAGGTTGTGGATATG
ATCGCCACTATCCAGCAGCACATTGATCAGGGAATCAGCTTTACATTGTTCTTAAAAGACACAATGACGACCCGAGATTT
AAACCGAATCGATCTGTATGCACATCATAGAGGAATTAAAACCATTTACTATGCAAGAACGAAAGATACTGGGCAAGACA
GCTGCCTTTCTTGTGTTGTTTGA

Upstream 100 bases:

>100_bases
GATACCATTTCAAGACAATATCAGGTGCCGATCTTGCACAAATTTGAACTCAGCGGCACATCTAAAGACGTTGAATTGTT
TACTCAGGAGGTAGAAAGAG

Downstream 100 bases:

>100_bases
TTAAAGGAGAGTTTATAGTGACAAAAATTTATGACGCAGCAAACTGGTCAAAGCATGAAGACGATTTTACCCAAATGTTC
TATAACCAAAACGTGAAACA

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase large subunit

Number of amino acids: Translated: 700; Mature: 699

Protein sequence:

>700_residues
MSQNQVPKWIQLNNEIMIQKDGKFQFDKDKEAVHSYFVDYINQNTVFFHNLKEKLDYLVENQYYEEEFLSLYSFEDIKEV
FKTAYAKKFRFPSFMSAFKFYNDYALKTNDKKKILERYEDRISIVALFFANGDTEKAKEYVNLMINQEYQPSTPTFLNAG
RKRRGELVSCFLLEVNDSLNDISRAIDISMQLSKLGGGVSLNLSKLRAKGEAIKDVENATKGVVGVMKLLDNAFRYADQM
GQRQGSGAAYLNIFHRDINDFLDTKKISADEDVRVKTLSIGVVIPDKFVELAREDKAAYVFYPHTIYKEYGQHMDEMDMN
EMYDKFVDNPRVKKEKINPRKLLEKLAMLRSESGYPYIMFQDNVNKVHANNHISKVKFSNLCSEVLQASQVSSYTDYDEE
DEIGLDISCNLGSLNILNVMEHKSIEKTVKLATDSLTHVSETTDIRNAPAVRRANKAMKSIGLGAMNLHGYLAQNGIAYE
SPEARDFANTFFMMVNFYSIQRSAEIAKEKGETFDQYEGSTYATGEYFDKYVSTDFSPKYEKIANLFEGMHIPTTEDWKK
LKAFVAEHGMYHSYRLCIAPTGSISYVQSSTASVMPIMERIEERTYGNSKTYYPMPGLASNNWFFYKEAYDMDMFKVVDM
IATIQQHIDQGISFTLFLKDTMTTRDLNRIDLYAHHRGIKTIYYARTKDTGQDSCLSCVV

Sequences:

>Translated_700_residues
MSQNQVPKWIQLNNEIMIQKDGKFQFDKDKEAVHSYFVDYINQNTVFFHNLKEKLDYLVENQYYEEEFLSLYSFEDIKEV
FKTAYAKKFRFPSFMSAFKFYNDYALKTNDKKKILERYEDRISIVALFFANGDTEKAKEYVNLMINQEYQPSTPTFLNAG
RKRRGELVSCFLLEVNDSLNDISRAIDISMQLSKLGGGVSLNLSKLRAKGEAIKDVENATKGVVGVMKLLDNAFRYADQM
GQRQGSGAAYLNIFHRDINDFLDTKKISADEDVRVKTLSIGVVIPDKFVELAREDKAAYVFYPHTIYKEYGQHMDEMDMN
EMYDKFVDNPRVKKEKINPRKLLEKLAMLRSESGYPYIMFQDNVNKVHANNHISKVKFSNLCSEVLQASQVSSYTDYDEE
DEIGLDISCNLGSLNILNVMEHKSIEKTVKLATDSLTHVSETTDIRNAPAVRRANKAMKSIGLGAMNLHGYLAQNGIAYE
SPEARDFANTFFMMVNFYSIQRSAEIAKEKGETFDQYEGSTYATGEYFDKYVSTDFSPKYEKIANLFEGMHIPTTEDWKK
LKAFVAEHGMYHSYRLCIAPTGSISYVQSSTASVMPIMERIEERTYGNSKTYYPMPGLASNNWFFYKEAYDMDMFKVVDM
IATIQQHIDQGISFTLFLKDTMTTRDLNRIDLYAHHRGIKTIYYARTKDTGQDSCLSCVV
>Mature_699_residues
SQNQVPKWIQLNNEIMIQKDGKFQFDKDKEAVHSYFVDYINQNTVFFHNLKEKLDYLVENQYYEEEFLSLYSFEDIKEVF
KTAYAKKFRFPSFMSAFKFYNDYALKTNDKKKILERYEDRISIVALFFANGDTEKAKEYVNLMINQEYQPSTPTFLNAGR
KRRGELVSCFLLEVNDSLNDISRAIDISMQLSKLGGGVSLNLSKLRAKGEAIKDVENATKGVVGVMKLLDNAFRYADQMG
QRQGSGAAYLNIFHRDINDFLDTKKISADEDVRVKTLSIGVVIPDKFVELAREDKAAYVFYPHTIYKEYGQHMDEMDMNE
MYDKFVDNPRVKKEKINPRKLLEKLAMLRSESGYPYIMFQDNVNKVHANNHISKVKFSNLCSEVLQASQVSSYTDYDEED
EIGLDISCNLGSLNILNVMEHKSIEKTVKLATDSLTHVSETTDIRNAPAVRRANKAMKSIGLGAMNLHGYLAQNGIAYES
PEARDFANTFFMMVNFYSIQRSAEIAKEKGETFDQYEGSTYATGEYFDKYVSTDFSPKYEKIANLFEGMHIPTTEDWKKL
KAFVAEHGMYHSYRLCIAPTGSISYVQSSTASVMPIMERIEERTYGNSKTYYPMPGLASNNWFFYKEAYDMDMFKVVDMI
ATIQQHIDQGISFTLFLKDTMTTRDLNRIDLYAHHRGIKTIYYARTKDTGQDSCLSCVV

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family

Homologues:

Organism=Homo sapiens, GI4506749, Length=628, Percent_Identity=25.4777070063694, Blast_Score=184, Evalue=2e-46,
Organism=Escherichia coli, GI1789030, Length=687, Percent_Identity=43.5225618631732, Blast_Score=629, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=663, Percent_Identity=23.3785822021116, Blast_Score=151, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17555242, Length=629, Percent_Identity=26.2321144674086, Blast_Score=198, Evalue=8e-51,
Organism=Saccharomyces cerevisiae, GI6322123, Length=654, Percent_Identity=24.9235474006116, Blast_Score=184, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6320914, Length=654, Percent_Identity=25.0764525993884, Blast_Score=179, Evalue=1e-45,
Organism=Drosophila melanogaster, GI17352481, Length=628, Percent_Identity=26.1146496815287, Blast_Score=192, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIR1_BACSU (P50620)

Other databases:

- EMBL:   Z68500
- EMBL:   AL009126
- PIR:   B69667
- RefSeq:   NP_389620.1
- ProteinModelPortal:   P50620
- SMR:   P50620
- EnsemblBacteria:   EBBACT00000003902
- GeneID:   940091
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU17380
- NMPDR:   fig|224308.1.peg.1742
- GenoList:   BSU17380
- GeneTree:   EBGT00050000001799
- HOGENOM:   HBG348953
- OMA:   DKCVIST
- PhylomeDB:   P50620
- ProtClustDB:   PRK07632
- BioCyc:   BSUB:BSU17380-MONOMER
- BRENDA:   1.17.4.1
- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554
- PANTHER:   PTHR11573
- PRINTS:   PR01183
- TIGRFAMs:   TIGR02506

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N; SSF48168 Ribonucleo_red_N

EC number: =1.17.4.1

Molecular weight: Translated: 80689; Mature: 80558

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00089 RIBORED_LARGE

Important sites: ACT_SITE 380-380 ACT_SITE 382-382 ACT_SITE 384-384 BINDING 153-153 BINDING 198-198

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNQVPKWIQLNNEIMIQKDGKFQFDKDKEAVHSYFVDYINQNTVFFHNLKEKLDYLVE
CCCCCCCHHEEECCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHH
NQYYEEEFLSLYSFEDIKEVFKTAYAKKFRFPSFMSAFKFYNDYALKTNDKKKILERYED
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEECCCCHHHHHHHHHH
RISIVALFFANGDTEKAKEYVNLMINQEYQPSTPTFLNAGRKRRGELVSCFLLEVNDSLN
HEEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHCCCEEEEEEEEECCCHH
DISRAIDISMQLSKLGGGVSLNLSKLRAKGEAIKDVENATKGVVGVMKLLDNAFRYADQM
HHHHHHHHHHHHHHCCCCEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GQRQGSGAAYLNIFHRDINDFLDTKKISADEDVRVKTLSIGVVIPDKFVELAREDKAAYV
HCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEECHHHHHHHHCCCEEEE
FYPHTIYKEYGQHMDEMDMNEMYDKFVDNPRVKKEKINPRKLLEKLAMLRSESGYPYIMF
EECHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHCCCCCEEEEE
QDNVNKVHANNHISKVKFSNLCSEVLQASQVSSYTDYDEEDEIGLDISCNLGSLNILNVM
ECCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHH
EHKSIEKTVKLATDSLTHVSETTDIRNAPAVRRANKAMKSIGLGAMNLHGYLAQNGIAYE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCC
SPEARDFANTFFMMVNFYSIQRSAEIAKEKGETFDQYEGSTYATGEYFDKYVSTDFSPKY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEECHHHHHHHHCCCCCCHH
EKIANLFEGMHIPTTEDWKKLKAFVAEHGMYHSYRLCIAPTGSISYVQSSTASVMPIMER
HHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHCCCHHHHHHHHH
IEERTYGNSKTYYPMPGLASNNWFFYKEAYDMDMFKVVDMIATIQQHIDQGISFTLFLKD
HHHHCCCCCCEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEC
TMTTRDLNRIDLYAHHRGIKTIYYARTKDTGQDSCLSCVV
CCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCHHHHHHCC
>Mature Secondary Structure 
SQNQVPKWIQLNNEIMIQKDGKFQFDKDKEAVHSYFVDYINQNTVFFHNLKEKLDYLVE
CCCCCCHHEEECCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHH
NQYYEEEFLSLYSFEDIKEVFKTAYAKKFRFPSFMSAFKFYNDYALKTNDKKKILERYED
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEECCCCHHHHHHHHHH
RISIVALFFANGDTEKAKEYVNLMINQEYQPSTPTFLNAGRKRRGELVSCFLLEVNDSLN
HEEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHCCCEEEEEEEEECCCHH
DISRAIDISMQLSKLGGGVSLNLSKLRAKGEAIKDVENATKGVVGVMKLLDNAFRYADQM
HHHHHHHHHHHHHHCCCCEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GQRQGSGAAYLNIFHRDINDFLDTKKISADEDVRVKTLSIGVVIPDKFVELAREDKAAYV
HCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEECHHHHHHHHCCCEEEE
FYPHTIYKEYGQHMDEMDMNEMYDKFVDNPRVKKEKINPRKLLEKLAMLRSESGYPYIMF
EECHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHCCCCCEEEEE
QDNVNKVHANNHISKVKFSNLCSEVLQASQVSSYTDYDEEDEIGLDISCNLGSLNILNVM
ECCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHH
EHKSIEKTVKLATDSLTHVSETTDIRNAPAVRRANKAMKSIGLGAMNLHGYLAQNGIAYE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCC
SPEARDFANTFFMMVNFYSIQRSAEIAKEKGETFDQYEGSTYATGEYFDKYVSTDFSPKY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEECHHHHHHHHCCCCCCHH
EKIANLFEGMHIPTTEDWKKLKAFVAEHGMYHSYRLCIAPTGSISYVQSSTASVMPIMER
HHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHCCCHHHHHHHHH
IEERTYGNSKTYYPMPGLASNNWFFYKEAYDMDMFKVVDMIATIQQHIDQGISFTLFLKD
HHHHCCCCCCEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEC
TMTTRDLNRIDLYAHHRGIKTIYYARTKDTGQDSCLSCVV
CCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969495; 9384377