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The map label for this gene is nrdF

Identifier: 16078802

GI number: 16078802

Start: 1871089

End: 1872078

Strand: Direct

Name: nrdF

Synonym: BSU17390

Alternate gene names: 16078802

Gene position: 1871089-1872078 (Clockwise)

Preceding gene: 16078801

Following gene: 16078803

Centisome position: 44.38

GC content: 41.41

Gene sequence:

>990_bases
GTGACAAAAATTTATGACGCAGCAAACTGGTCAAAGCATGAAGACGATTTTACCCAAATGTTCTATAACCAAAACGTGAA
ACAGTTCTGGCTTCCGGAAGAGATTGCTTTAAACGGCGATCTCCTCACATGGAAGTACCTCGGAAAAAATGAGCAGGACA
CTTATATGAAGGTACTGGCCGGACTTACGCTTCTTGACACAGAGCAGGGGAATACGGGGATGCCGATCGTGGCTGAACAC
GTAGATGGCCACCAGCGGAAAGCGGTGCTGAACTTTATGGCGATGATGGAGAACGCTGTCCATGCGAAGTCGTACTCTAA
TATTTTCATGACCCTTGCACCGACTGAAACGATCAATGAAGTATTTGAATGGGTTAAACAAAATAAATATTTGCAGAAAA
AAGCGCAAATGATTGTCGGGCTTTACAAAGCCATTCAAAAAGATGATGAAATTTCACTGTTCAAAGCCATGGTTGCTTCC
GTCTATCTGGAAAGCTTCCTTTTCTACAGCGGTTTCTATTATCCGTTATATTTCTACGGACAAGGAAAACTGATGCAAAG
CGGTGAGATCATTAACCTGATCCTTCGTGACGAAGCGATTCACGGCGTGTATGTCGGTCTCCTTGCTCAGGAAATTTATA
ATAAACAGACAGAAGAGAAAAAAGCAGAGCTTCGTGAATTTGCGATCGACCTCCTGAATCAGTTATATGAAAATGAGCTT
GAGTATACAGAGGACTTGTACGATCAAGTCGGGCTCTCACATGATGTAAAGAAATTCATCCGCTATAATGCGAATAAAGC
GCTGATGAACCTTGGATTTGATCCATACTTCGAAGAGGAAGACATTAATCCGATCGTTTTAAACGGATTAAACACAAAAA
CAAAATCGCATGACTTCTTCTCTATGAAGGGGAATGGCTATAAAAAAGCGACAGTTGAGCCGCTCAAAGATGATGACTTT
TATTTTGAAGATGAAAAAGAGCAGATATAA

Upstream 100 bases:

>100_bases
CACATCATAGAGGAATTAAAACCATTTACTATGCAAGAACGAAAGATACTGGGCAAGACAGCTGCCTTTCTTGTGTTGTT
TGATTAAAGGAGAGTTTATA

Downstream 100 bases:

>100_bases
TATCTGCTCTTTTCGGCTTGAAGGTTCAAATGAGGAGGACGCAGTACCCATGGGAAAAATGGACGAAATGATTTTAGTTG
CCCCGCGCGATGATGTGTTT

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MTKIYDAANWSKHEDDFTQMFYNQNVKQFWLPEEIALNGDLLTWKYLGKNEQDTYMKVLAGLTLLDTEQGNTGMPIVAEH
VDGHQRKAVLNFMAMMENAVHAKSYSNIFMTLAPTETINEVFEWVKQNKYLQKKAQMIVGLYKAIQKDDEISLFKAMVAS
VYLESFLFYSGFYYPLYFYGQGKLMQSGEIINLILRDEAIHGVYVGLLAQEIYNKQTEEKKAELREFAIDLLNQLYENEL
EYTEDLYDQVGLSHDVKKFIRYNANKALMNLGFDPYFEEEDINPIVLNGLNTKTKSHDFFSMKGNGYKKATVEPLKDDDF
YFEDEKEQI

Sequences:

>Translated_329_residues
MTKIYDAANWSKHEDDFTQMFYNQNVKQFWLPEEIALNGDLLTWKYLGKNEQDTYMKVLAGLTLLDTEQGNTGMPIVAEH
VDGHQRKAVLNFMAMMENAVHAKSYSNIFMTLAPTETINEVFEWVKQNKYLQKKAQMIVGLYKAIQKDDEISLFKAMVAS
VYLESFLFYSGFYYPLYFYGQGKLMQSGEIINLILRDEAIHGVYVGLLAQEIYNKQTEEKKAELREFAIDLLNQLYENEL
EYTEDLYDQVGLSHDVKKFIRYNANKALMNLGFDPYFEEEDINPIVLNGLNTKTKSHDFFSMKGNGYKKATVEPLKDDDF
YFEDEKEQI
>Mature_328_residues
TKIYDAANWSKHEDDFTQMFYNQNVKQFWLPEEIALNGDLLTWKYLGKNEQDTYMKVLAGLTLLDTEQGNTGMPIVAEHV
DGHQRKAVLNFMAMMENAVHAKSYSNIFMTLAPTETINEVFEWVKQNKYLQKKAQMIVGLYKAIQKDDEISLFKAMVASV
YLESFLFYSGFYYPLYFYGQGKLMQSGEIINLILRDEAIHGVYVGLLAQEIYNKQTEEKKAELREFAIDLLNQLYENELE
YTEDLYDQVGLSHDVKKFIRYNANKALMNLGFDPYFEEEDINPIVLNGLNTKTKSHDFFSMKGNGYKKATVEPLKDDDFY
FEDEKEQI

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family

Homologues:

Organism=Homo sapiens, GI42544136, Length=298, Percent_Identity=26.510067114094, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI289177074, Length=298, Percent_Identity=26.510067114094, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1789031, Length=319, Percent_Identity=43.5736677115987, Blast_Score=259, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI17551968, Length=305, Percent_Identity=23.2786885245902, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIR2_BACSU (P50621)

Other databases:

- EMBL:   Z68500
- EMBL:   AL009126
- PIR:   C69667
- RefSeq:   NP_389621.1
- ProteinModelPortal:   P50621
- SMR:   P50621
- EnsemblBacteria:   EBBACT00000002155
- GeneID:   940076
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU17390
- NMPDR:   fig|224308.1.peg.1743
- GenoList:   BSU17390
- GeneTree:   EBGT00050000002650
- HOGENOM:   HBG516660
- OMA:   TLCSTPD
- PhylomeDB:   P50621
- ProtClustDB:   PRK09614
- BioCyc:   BSUB:BSU17390-MONOMER
- BRENDA:   1.17.4.1
- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358
- Gene3D:   G3DSA:1.10.620.20
- PANTHER:   PTHR23409

Pfam domain/function: PF00268 Ribonuc_red_sm; SSF47240 Ferritin/RR_like

EC number: =1.17.4.1

Molecular weight: Translated: 38379; Mature: 38248

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00368 RIBORED_SMALL

Important sites: ACT_SITE 105-105

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIYDAANWSKHEDDFTQMFYNQNVKQFWLPEEIALNGDLLTWKYLGKNEQDTYMKVLA
CCCCCCCCCCCCCHHHHHHHHHHCCCHHHCCCHHHEECCCEEEEEECCCCCHHHHHHHHH
GLTLLDTEQGNTGMPIVAEHVDGHQRKAVLNFMAMMENAVHAKSYSNIFMTLAPTETINE
CCCEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHH
VFEWVKQNKYLQKKAQMIVGLYKAIQKDDEISLFKAMVASVYLESFLFYSGFYYPLYFYG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEE
QGKLMQSGEIINLILRDEAIHGVYVGLLAQEIYNKQTEEKKAELREFAIDLLNQLYENEL
CCCEECCCCEEEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
EYTEDLYDQVGLSHDVKKFIRYNANKALMNLGFDPYFEEEDINPIVLNGLNTKTKSHDFF
HHHHHHHHHHCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCEE
SMKGNGYKKATVEPLKDDDFYFEDEKEQI
EECCCCCCCCCCCCCCCCCCEECCHHCCC
>Mature Secondary Structure 
TKIYDAANWSKHEDDFTQMFYNQNVKQFWLPEEIALNGDLLTWKYLGKNEQDTYMKVLA
CCCCCCCCCCCCHHHHHHHHHHCCCHHHCCCHHHEECCCEEEEEECCCCCHHHHHHHHH
GLTLLDTEQGNTGMPIVAEHVDGHQRKAVLNFMAMMENAVHAKSYSNIFMTLAPTETINE
CCCEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHH
VFEWVKQNKYLQKKAQMIVGLYKAIQKDDEISLFKAMVASVYLESFLFYSGFYYPLYFYG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEE
QGKLMQSGEIINLILRDEAIHGVYVGLLAQEIYNKQTEEKKAELREFAIDLLNQLYENEL
CCCEECCCCEEEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
EYTEDLYDQVGLSHDVKKFIRYNANKALMNLGFDPYFEEEDINPIVLNGLNTKTKSHDFF
HHHHHHHHHHCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCEE
SMKGNGYKKATVEPLKDDDFYFEDEKEQI
EECCCCCCCCCCCCCCCCCCEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969495; 9384377