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The map label for this gene is truB

Identifier: 16078729

GI number: 16078729

Start: 1736886

End: 1737815

Strand: Direct

Name: truB

Synonym: BSU16660

Alternate gene names: 16078729

Gene position: 1736886-1737815 (Clockwise)

Preceding gene: 16078728

Following gene: 16078730

Centisome position: 41.2

GC content: 43.12

Gene sequence:

>930_bases
ATGGTTAACGGAGTTCTCCTTTTACATAAACCTGTTGGCATGACATCGCACGACTGTGTCATGAAGATCCGAAAGCTGTT
AAAAACAAAAAAAGTAGGACATACAGGAACGCTCGATCCGGAAGTATCAGGCGTGCTTCCGATTTGTGTAGGAAGAGCGA
CAAAAATAGTCGAATACTTAACTGAAAAATCTAAAACGTATGATGCAGAAATAACATTGGGCTTTTCAACCACAACAGAG
GATCAAACCGGAGAAACGGTGGAAACAAAGCCTGTCAATCACGACATTGATAAAGCGGATGTAGAAAAGGTTTTAAACAG
TTTAAAGGGGAAACAAGAACAAATTCCGCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAATACGCAA
GAGCAGGGATTGAAGTAGAACGTCCGAAACGTATGATAACCATTGAAGACATTGCCCTGACAACAGAGATTAAACATCAT
GGAGAAACCGCAAGCTTCCGGTTTACAGTGACTTGCTCCAAAGGGACCTATGTAAGGACGCTGGCGGTCATGATCGGAGA
AAAGCTCGGATATCCGGCCCATATGTCTCATTTAATCCGTACGGCATCCGGAGATTTTTCTCTCGACGAATGCTTTACTT
TTGATGAACTCGAAGCACAGGCCCAATCCGGAACCGTGGAAGAGCATACAGTTCCTATTGAACGTGCGCTCAATCATTTG
CCGAAATGGATCATAAGTGATACATTAGCTAAGAAAGTAGAAAATGGGGCTTTGCTTGAGACGCCCGAGCAGTTTTCTGA
AATGACAAGTGGAGACCGTATTGCCGTCTTTACTGAATCCGGAACCTGCTTGGCGATCTATTTCCCTCATCCTGCAAAAA
AAGGGCTGTTAAAGCCGGCAAAAGTATTGATGCAAAAAAGCGAACAATAG

Upstream 100 bases:

>100_bases
AGAGAAACCATCTGAATAAAAAAAGAGAGGATAGGCGTATATCGTCTGTCCTCTTTCTTCGTTTATAAGAAAAAAACTCA
AAAGAAGGAGTGAAAGGCAA

Downstream 100 bases:

>100_bases
AAAAAAGGTGACCGTTCTGTGAAGACGATACATATTACACATCCTCATCATTTAATAAAAGAGGAGCAGGCAAAATCAGT
GATGGCGTTAGGTTATTTTG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MVNGVLLLHKPVGMTSHDCVMKIRKLLKTKKVGHTGTLDPEVSGVLPICVGRATKIVEYLTEKSKTYDAEITLGFSTTTE
DQTGETVETKPVNHDIDKADVEKVLNSLKGKQEQIPPMYSAVKVNGKKLYEYARAGIEVERPKRMITIEDIALTTEIKHH
GETASFRFTVTCSKGTYVRTLAVMIGEKLGYPAHMSHLIRTASGDFSLDECFTFDELEAQAQSGTVEEHTVPIERALNHL
PKWIISDTLAKKVENGALLETPEQFSEMTSGDRIAVFTESGTCLAIYFPHPAKKGLLKPAKVLMQKSEQ

Sequences:

>Translated_309_residues
MVNGVLLLHKPVGMTSHDCVMKIRKLLKTKKVGHTGTLDPEVSGVLPICVGRATKIVEYLTEKSKTYDAEITLGFSTTTE
DQTGETVETKPVNHDIDKADVEKVLNSLKGKQEQIPPMYSAVKVNGKKLYEYARAGIEVERPKRMITIEDIALTTEIKHH
GETASFRFTVTCSKGTYVRTLAVMIGEKLGYPAHMSHLIRTASGDFSLDECFTFDELEAQAQSGTVEEHTVPIERALNHL
PKWIISDTLAKKVENGALLETPEQFSEMTSGDRIAVFTESGTCLAIYFPHPAKKGLLKPAKVLMQKSEQ
>Mature_309_residues
MVNGVLLLHKPVGMTSHDCVMKIRKLLKTKKVGHTGTLDPEVSGVLPICVGRATKIVEYLTEKSKTYDAEITLGFSTTTE
DQTGETVETKPVNHDIDKADVEKVLNSLKGKQEQIPPMYSAVKVNGKKLYEYARAGIEVERPKRMITIEDIALTTEIKHH
GETASFRFTVTCSKGTYVRTLAVMIGEKLGYPAHMSHLIRTASGDFSLDECFTFDELEAQAQSGTVEEHTVPIERALNHL
PKWIISDTLAKKVENGALLETPEQFSEMTSGDRIAVFTESGTCLAIYFPHPAKKGLLKPAKVLMQKSEQ

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=226, Percent_Identity=34.9557522123894, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI215599015, Length=298, Percent_Identity=28.5234899328859, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI4503337, Length=296, Percent_Identity=28.7162162162162, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI2367200, Length=239, Percent_Identity=41.0041841004184, Blast_Score=184, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI17553978, Length=297, Percent_Identity=28.6195286195286, Blast_Score=91, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6323204, Length=299, Percent_Identity=28.0936454849498, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324037, Length=195, Percent_Identity=33.3333333333333, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI281364189, Length=303, Percent_Identity=29.3729372937294, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI281364187, Length=303, Percent_Identity=29.3729372937294, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI281364185, Length=303, Percent_Identity=29.3729372937294, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI281364183, Length=303, Percent_Identity=29.3729372937294, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI62471759, Length=303, Percent_Identity=29.3729372937294, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI17975520, Length=303, Percent_Identity=29.3729372937294, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_BACSU (P32732)

Other databases:

- EMBL:   AL009126
- EMBL:   Z18631
- PIR:   G69726
- RefSeq:   NP_389548.1
- ProteinModelPortal:   P32732
- SMR:   P32732
- EnsemblBacteria:   EBBACT00000001635
- GeneID:   939638
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU16660
- NMPDR:   fig|224308.1.peg.1669
- GenoList:   BSU16660
- GeneTree:   EBGT00050000002629
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- PhylomeDB:   P32732
- ProtClustDB:   PRK01550
- BioCyc:   BSUB:BSU16660-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 34205; Mature: 34205

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: ACT_SITE 39-39

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNGVLLLHKPVGMTSHDCVMKIRKLLKTKKVGHTGTLDPEVSGVLPICVGRATKIVEYL
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
TEKSKTYDAEITLGFSTTTEDQTGETVETKPVNHDIDKADVEKVLNSLKGKQEQIPPMYS
HHCCCCEEEEEEEEECCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHCCCHHHCCCHHH
AVKVNGKKLYEYARAGIEVERPKRMITIEDIALTTEIKHHGETASFRFTVTCSKGTYVRT
HEEECHHHHHHHHHCCCEECCCCEEEEEEHHEEEHHHHHCCCCEEEEEEEEECCCCHHHH
LAVMIGEKLGYPAHMSHLIRTASGDFSLDECFTFDELEAQAQSGTVEEHTVPIERALNHL
HHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHCCCCHHHCCCHHHHHHHH
PKWIISDTLAKKVENGALLETPEQFSEMTSGDRIAVFTESGTCLAIYFPHPAKKGLLKPA
HHHHHHHHHHHHHCCCCEECCHHHHHHHCCCCEEEEEECCCCEEEEECCCCCCCCCCHHH
KVLMQKSEQ
HHHHHCCCC
>Mature Secondary Structure
MVNGVLLLHKPVGMTSHDCVMKIRKLLKTKKVGHTGTLDPEVSGVLPICVGRATKIVEYL
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
TEKSKTYDAEITLGFSTTTEDQTGETVETKPVNHDIDKADVEKVLNSLKGKQEQIPPMYS
HHCCCCEEEEEEEEECCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHCCCHHHCCCHHH
AVKVNGKKLYEYARAGIEVERPKRMITIEDIALTTEIKHHGETASFRFTVTCSKGTYVRT
HEEECHHHHHHHHHCCCEECCCCEEEEEEHHEEEHHHHHCCCCEEEEEEEEECCCCHHHH
LAVMIGEKLGYPAHMSHLIRTASGDFSLDECFTFDELEAQAQSGTVEEHTVPIERALNHL
HHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHCCCCHHHCCCHHHHHHHH
PKWIISDTLAKKVENGALLETPEQFSEMTSGDRIAVFTESGTCLAIYFPHPAKKGLLKPA
HHHHHHHHHHHHHCCCCEECCHHHHHHHCCCCEEEEEECCCCEEEEECCCCCCCCCCHHH
KVLMQKSEQ
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 8491709