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The map label for this gene is nusA

Identifier: 16078723

GI number: 16078723

Start: 1732281

End: 1733396

Strand: Direct

Name: nusA

Synonym: BSU16600

Alternate gene names: 16078723

Gene position: 1732281-1733396 (Clockwise)

Preceding gene: 16078722

Following gene: 16078724

Centisome position: 41.09

GC content: 43.73

Gene sequence:

>1116_bases
ATGAGCAGTGAATTATTAGATGCTCTCACGATTCTTGAAAAAGAAAAAGGCATCAGTAAAGAAATTATCATCGAAGCAAT
CGAAGCTGCGTTAATTTCTGCTTATAAGCGGAATTTTAATCAAGCGCAAAATGTACGGGTGGATCTAAACCGTGAAACTG
GCTCCATCCGTGTATTTGCAAGAAAAGATGTCGTTGACGAAGTATACGACCAGAGATTAGAAATCTCAATTGAAGAGGCA
CAGGGCATCCATCCGGAATATATGGTCGGCGATGTCGTCGAAATCGAAGTTACACCTAAGGATTTCGGGCGCATAGCTGC
TCAAACAGCGAAGCAGGTCGTCACACAGCGTGTGCGCGAAGCTGAGCGCGGTGTGATTTATTCTGAATTTATCGACCGTG
AAGAAGACATCATGACAGGGATCGTCCAGCGTCTGGACAATAAATTTATCTACGTGTCTTTAGGCAAAATAGAAGCCTTG
CTGCCGGTTAATGAGCAAATGCCGAATGAAAGCTACAAGCCCCATGACCGCATTAAGGTATATATCACAAAAGTAGAAAA
AACAACGAAGGGTCCGCAGATTTATGTGTCAAGAACACATCCAGGCCTGTTAAAGCGTTTGTTTGAAATTGAAGTGCCTG
AAATTTATGATGGAACTGTCGAGCTAAAATCTGTTGCCAGAGAAGCCGGCGACCGTTCGAAAATTTCTGTTCGCACAGAT
GATCCTGACGTTGATCCTGTCGGTTCATGCGTAGGGCCTAAAGGCCAGCGTGTGCAAGCAATCGTCAATGAATTAAAAGG
TGAAAAAATTGACATTGTCAACTGGTCCAGTGATCCTGTAGAATTTGTCGCGAATGCGCTCAGCCCTTCCAAAGTGCTTG
ATGTCATTGTTAATGAAGAAGAAAAAGCGACAACTGTTATTGTTCCTGATTACCAGCTGTCTCTGGCAATTGGAAAAAGA
GGACAAAACGCACGCTTAGCTGCTAAACTGACAGGCTGGAAGATTGATATTAAGAGCGAAACGGACGCAAGAGAACTTGG
AATCTATCCGAGAGAACTTGAAGAAGATGATGAGCCGCTCTTTACGGAGCCTGAAACTGCTGAATCGGATGAATAA

Upstream 100 bases:

>100_bases
AGAAAGAAACGGATCAATATTCCGTATGAAAAGATAGCTAATGCAAGATTAGCTGTTACATTCTAATGAATACAAATGTT
TTAAGGGGGGAGACCGTTAA

Downstream 100 bases:

>100_bases
GAGGTGACTTTAGGTGAATAAACACAAAAAGATCCCATTACGCAAATGTGTAGTGACTGGTGAAATGAAGCCTAAAAAGG
AACTGATCCGAGTTGTACGG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MSSELLDALTILEKEKGISKEIIIEAIEAALISAYKRNFNQAQNVRVDLNRETGSIRVFARKDVVDEVYDQRLEISIEEA
QGIHPEYMVGDVVEIEVTPKDFGRIAAQTAKQVVTQRVREAERGVIYSEFIDREEDIMTGIVQRLDNKFIYVSLGKIEAL
LPVNEQMPNESYKPHDRIKVYITKVEKTTKGPQIYVSRTHPGLLKRLFEIEVPEIYDGTVELKSVAREAGDRSKISVRTD
DPDVDPVGSCVGPKGQRVQAIVNELKGEKIDIVNWSSDPVEFVANALSPSKVLDVIVNEEEKATTVIVPDYQLSLAIGKR
GQNARLAAKLTGWKIDIKSETDARELGIYPRELEEDDEPLFTEPETAESDE

Sequences:

>Translated_371_residues
MSSELLDALTILEKEKGISKEIIIEAIEAALISAYKRNFNQAQNVRVDLNRETGSIRVFARKDVVDEVYDQRLEISIEEA
QGIHPEYMVGDVVEIEVTPKDFGRIAAQTAKQVVTQRVREAERGVIYSEFIDREEDIMTGIVQRLDNKFIYVSLGKIEAL
LPVNEQMPNESYKPHDRIKVYITKVEKTTKGPQIYVSRTHPGLLKRLFEIEVPEIYDGTVELKSVAREAGDRSKISVRTD
DPDVDPVGSCVGPKGQRVQAIVNELKGEKIDIVNWSSDPVEFVANALSPSKVLDVIVNEEEKATTVIVPDYQLSLAIGKR
GQNARLAAKLTGWKIDIKSETDARELGIYPRELEEDDEPLFTEPETAESDE
>Mature_370_residues
SSELLDALTILEKEKGISKEIIIEAIEAALISAYKRNFNQAQNVRVDLNRETGSIRVFARKDVVDEVYDQRLEISIEEAQ
GIHPEYMVGDVVEIEVTPKDFGRIAAQTAKQVVTQRVREAERGVIYSEFIDREEDIMTGIVQRLDNKFIYVSLGKIEALL
PVNEQMPNESYKPHDRIKVYITKVEKTTKGPQIYVSRTHPGLLKRLFEIEVPEIYDGTVELKSVAREAGDRSKISVRTDD
PDVDPVGSCVGPKGQRVQAIVNELKGEKIDIVNWSSDPVEFVANALSPSKVLDVIVNEEEKATTVIVPDYQLSLAIGKRG
QNARLAAKLTGWKIDIKSETDARELGIYPRELEEDDEPLFTEPETAESDE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Escherichia coli, GI1789560, Length=343, Percent_Identity=40.2332361516035, Blast_Score=275, Evalue=4e-75,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NUSA_BACSU (P32727)

Other databases:

- EMBL:   Z18631
- EMBL:   AL009126
- PIR:   C36905
- RefSeq:   NP_389542.1
- ProteinModelPortal:   P32727
- SMR:   P32727
- EnsemblBacteria:   EBBACT00000003975
- GeneID:   939628
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU16600
- NMPDR:   fig|224308.1.peg.1663
- GenoList:   BSU16600
- GeneTree:   EBGT00050000001690
- HOGENOM:   HBG529269
- OMA:   RSKISVY
- PhylomeDB:   P32727
- ProtClustDB:   PRK12327
- BioCyc:   BSUB:BSU16600-MONOMER
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213
- Gene3D:   G3DSA:3.30.300.20
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.30.1480.10
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR01953

Pfam domain/function: PF08529 NusA_N; PF00575 S1; SSF54814 KH_prok; SSF50249 Nucleic_acid_OB; SSF69705 TF_NusA_N

EC number: NA

Molecular weight: Translated: 41726; Mature: 41595

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSELLDALTILEKEKGISKEIIIEAIEAALISAYKRNFNQAQNVRVDLNRETGSIRVFA
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEE
RKDVVDEVYDQRLEISIEEAQGIHPEYMVGDVVEIEVTPKDFGRIAAQTAKQVVTQRVRE
HHHHHHHHHHHHEEEEEHHHCCCCCCCEECCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
AERGVIYSEFIDREEDIMTGIVQRLDNKFIYVSLGKIEALLPVNEQMPNESYKPHDRIKV
HHCCEEHHHHHCCHHHHHHHHHHHHCCCEEEEEECCEEEEECCCCCCCCCCCCCCCCEEE
YITKVEKTTKGPQIYVSRTHPGLLKRLFEIEVPEIYDGTVELKSVAREAGDRSKISVRTD
EEEEEECCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEECC
DPDVDPVGSCVGPKGQRVQAIVNELKGEKIDIVNWSSDPVEFVANALSPSKVLDVIVNEE
CCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCHHHHHHHHCCCC
EKATTVIVPDYQLSLAIGKRGQNARLAAKLTGWKIDIKSETDARELGIYPRELEEDDEPL
CCCEEEEECCCEEEEEECCCCCCCEEEEEEECEEEEECCCCCHHHHCCCCHHCCCCCCCC
FTEPETAESDE
CCCCCCCCCCC
>Mature Secondary Structure 
SSELLDALTILEKEKGISKEIIIEAIEAALISAYKRNFNQAQNVRVDLNRETGSIRVFA
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEE
RKDVVDEVYDQRLEISIEEAQGIHPEYMVGDVVEIEVTPKDFGRIAAQTAKQVVTQRVRE
HHHHHHHHHHHHEEEEEHHHCCCCCCCEECCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
AERGVIYSEFIDREEDIMTGIVQRLDNKFIYVSLGKIEALLPVNEQMPNESYKPHDRIKV
HHCCEEHHHHHCCHHHHHHHHHHHHCCCEEEEEECCEEEEECCCCCCCCCCCCCCCCEEE
YITKVEKTTKGPQIYVSRTHPGLLKRLFEIEVPEIYDGTVELKSVAREAGDRSKISVRTD
EEEEEECCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEECC
DPDVDPVGSCVGPKGQRVQAIVNELKGEKIDIVNWSSDPVEFVANALSPSKVLDVIVNEE
CCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCHHHHHHHHCCCC
EKATTVIVPDYQLSLAIGKRGQNARLAAKLTGWKIDIKSETDARELGIYPRELEEDDEPL
CCCEEEEECCCEEEEEECCCCCCCEEEEEEECEEEEECCCCCHHHHCCCCHHCCCCCCCC
FTEPETAESDE
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8491709; 9384377