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The map label for this gene is topA

Identifier: 16078675

GI number: 16078675

Start: 1683661

End: 1685736

Strand: Direct

Name: topA

Synonym: BSU16120

Alternate gene names: 16078675

Gene position: 1683661-1685736 (Clockwise)

Preceding gene: 16078674

Following gene: 16078676

Centisome position: 39.94

GC content: 42.63

Gene sequence:

>2076_bases
ATGTCTGATTATCTAGTCATCGTGGAATCGCCCGCTAAGGCGAAAACGATTGAACGTTACTTAGGTAAAAAATATAAAGT
AAAAGCATCAATGGGACATGTCCGGGATCTTCCAAAAAGTCAAATGGGAGTTGACATAGAACAGAATTTTGAACCGAAAT
ATATTACCATTCGAGGTAAAGGCCCGGTATTAAAAGAGCTGAAAACGGCTGCGAAAAAAGCCAAAAAAGTGTATCTCGCA
GCTGACCCCGACAGAGAAGGGGAAGCGATTGCATGGCATTTGGCACACAGTCTTGATTTAGATCTCAACTCAGACTGCCG
TGTGGTGTTTAACGAAATTACAAAAGACGCTATTAAGGAATCGTTTAAGCATCCCCGCATGATCAATATGGATTTAGTGG
ATGCACAGCAAGCGAGACGTATTTTAGACAGGCTTGTCGGATATAAAATCAGTCCAATCTTATGGAAAAAAGTCAAAAAA
GGGCTTAGCGCAGGACGCGTGCAATCCGTTGCCCTCCGTTTGATTATTGACCGTGAAAAAGAGATTAACGACTTTAAGCC
TGAGGAATATTGGACAATTGACGGTACGTTCTTAAAAGGTCAAGAAACCTTTGAAGCGAGCTTTTTCGGGAAAAACGGCA
AAAAACTTCCTTTAAATAGTGAAGCTGATGTAAAAGAGATTCTTTCTCAGCTCAAAGGGAATCAATATACAGTTGAAAAA
GTAACCAAAAAGGAACGCAAACGTAATCCTGCTTTGCCTTTTACCACTTCTACCCTGCAGCAGGAAGCGGCTCGCAAGCT
CAATTTCAGAGCGAAGAAAACGATGATGATTGCACAGCAATTATATGAAGGAATTGATTTAGGAAGAGAAGGAACGGTTG
GTCTGATCACGTATATGAGAACGGATTCAACCCGTATTTCAAATACGGCTGTTGATGAAGCAGCTGCATTTATTGATCAG
ACATATGGAAAAGAGTTCCTAGGCGGAAAACGGAAGCCTGCGAAAAAGAATGAAAATGCCCAGGATGCCCACGAAGCAAT
TCGGCCGACATCAGTTCTTAGAAAACCAAGTGAATTAAAAGCAGTCCTCGGCAGAGACCAAATGAGACTGTATAAATTAA
TTTGGGAGCGTTTTGTTGCCAGCCAAATGGCACCGGCTGTTCTCGATACAATGAGTGTCGACCTGACAAACAACGGTTTG
ACATTTCGTGCAAATGGAAGTAAAGTCAAGTTTTCCGGGTTTATGAAGGTGTATGTTGAAGGAAAAGACGATCAAATGGA
AGAAAAAGACCGGATGCTGCCTGACTTACAAGAAGGCGACACGGTATTATCAAAAGATATAGAACCTGAGCAGCATTTTA
CCCAGCCGCCTCCGCGATATACTGAGGCCCGGCTTGTTAAAACCCTTGAAGAACGCGGTATCGGCCGTCCATCCACATAT
GCTCCGACACTTGATACTATTCAGCGGCGCGGCTACGTGGCATTGGATAATAAACGTTTCGTTCCGACTGAATTAGGTCA
GATCGTTCTTGACTTGATCATGGAGTTTTTCCCTGAGATCATTAACGTTGAGTTTACAGCTAAAATGGAGAGAGATCTTG
ATCATGTTGAAGAAGGAAATACCGAATGGGTCAAGATTATCGATAATTTCTACACCGATTTTGAAAAACGCGTCAAAAAA
GCCGAATCGGAAATGAAGGAAGTTGAGATTGAACCAGAGTATGCTGGAGAGGATTGTGAATTGTGCAGTTCTCCAATGGT
ATATAAAATGGGACGGTACGGTAAATTCTTAGCTTGCTCCAACTTCCCTGACTGCCGGAACACGAAACCGATTGTGAAAC
AAATCGGTGTGAAGTGCCCGAGCTGCGGAGAAGGAAACATTGTTGAGCGAAAATCGAAAAAGAAACGGGTGTTTTACGGC
TGTGACCGTTATCCGGACTGTGAATTCGTATCATGGGACAAACCAATTGAACGAAAATGCCCGAAATGCGGAAAAATGCT
CGTCGAGAAAAAACTCAAAAAAGGTATACAAGTCCAATGCGTGGAATGCGATTATAAGGAAGAACCACAGAAGTAG

Upstream 100 bases:

>100_bases
ATGATAAAGAATGAAATGTTGAAAGCCAAATAAAGACAGCTGCAAAAATAAAATTGAAATATCTTGAAAAGAGAGAGAAC
ACCTCTTGAGGGGGATGAAA

Downstream 100 bases:

>100_bases
CGGTGAGCAGGGTCTTGCTCACCTTCTTTGTGTGTTAAAAGAAGGCCTTGAATAATAAACTAGGAGATGTGAAAGATGAA
CCAACAAACAGTGAATGTAA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme

Number of amino acids: Translated: 691; Mature: 690

Protein sequence:

>691_residues
MSDYLVIVESPAKAKTIERYLGKKYKVKASMGHVRDLPKSQMGVDIEQNFEPKYITIRGKGPVLKELKTAAKKAKKVYLA
ADPDREGEAIAWHLAHSLDLDLNSDCRVVFNEITKDAIKESFKHPRMINMDLVDAQQARRILDRLVGYKISPILWKKVKK
GLSAGRVQSVALRLIIDREKEINDFKPEEYWTIDGTFLKGQETFEASFFGKNGKKLPLNSEADVKEILSQLKGNQYTVEK
VTKKERKRNPALPFTTSTLQQEAARKLNFRAKKTMMIAQQLYEGIDLGREGTVGLITYMRTDSTRISNTAVDEAAAFIDQ
TYGKEFLGGKRKPAKKNENAQDAHEAIRPTSVLRKPSELKAVLGRDQMRLYKLIWERFVASQMAPAVLDTMSVDLTNNGL
TFRANGSKVKFSGFMKVYVEGKDDQMEEKDRMLPDLQEGDTVLSKDIEPEQHFTQPPPRYTEARLVKTLEERGIGRPSTY
APTLDTIQRRGYVALDNKRFVPTELGQIVLDLIMEFFPEIINVEFTAKMERDLDHVEEGNTEWVKIIDNFYTDFEKRVKK
AESEMKEVEIEPEYAGEDCELCSSPMVYKMGRYGKFLACSNFPDCRNTKPIVKQIGVKCPSCGEGNIVERKSKKKRVFYG
CDRYPDCEFVSWDKPIERKCPKCGKMLVEKKLKKGIQVQCVECDYKEEPQK

Sequences:

>Translated_691_residues
MSDYLVIVESPAKAKTIERYLGKKYKVKASMGHVRDLPKSQMGVDIEQNFEPKYITIRGKGPVLKELKTAAKKAKKVYLA
ADPDREGEAIAWHLAHSLDLDLNSDCRVVFNEITKDAIKESFKHPRMINMDLVDAQQARRILDRLVGYKISPILWKKVKK
GLSAGRVQSVALRLIIDREKEINDFKPEEYWTIDGTFLKGQETFEASFFGKNGKKLPLNSEADVKEILSQLKGNQYTVEK
VTKKERKRNPALPFTTSTLQQEAARKLNFRAKKTMMIAQQLYEGIDLGREGTVGLITYMRTDSTRISNTAVDEAAAFIDQ
TYGKEFLGGKRKPAKKNENAQDAHEAIRPTSVLRKPSELKAVLGRDQMRLYKLIWERFVASQMAPAVLDTMSVDLTNNGL
TFRANGSKVKFSGFMKVYVEGKDDQMEEKDRMLPDLQEGDTVLSKDIEPEQHFTQPPPRYTEARLVKTLEERGIGRPSTY
APTLDTIQRRGYVALDNKRFVPTELGQIVLDLIMEFFPEIINVEFTAKMERDLDHVEEGNTEWVKIIDNFYTDFEKRVKK
AESEMKEVEIEPEYAGEDCELCSSPMVYKMGRYGKFLACSNFPDCRNTKPIVKQIGVKCPSCGEGNIVERKSKKKRVFYG
CDRYPDCEFVSWDKPIERKCPKCGKMLVEKKLKKGIQVQCVECDYKEEPQK
>Mature_690_residues
SDYLVIVESPAKAKTIERYLGKKYKVKASMGHVRDLPKSQMGVDIEQNFEPKYITIRGKGPVLKELKTAAKKAKKVYLAA
DPDREGEAIAWHLAHSLDLDLNSDCRVVFNEITKDAIKESFKHPRMINMDLVDAQQARRILDRLVGYKISPILWKKVKKG
LSAGRVQSVALRLIIDREKEINDFKPEEYWTIDGTFLKGQETFEASFFGKNGKKLPLNSEADVKEILSQLKGNQYTVEKV
TKKERKRNPALPFTTSTLQQEAARKLNFRAKKTMMIAQQLYEGIDLGREGTVGLITYMRTDSTRISNTAVDEAAAFIDQT
YGKEFLGGKRKPAKKNENAQDAHEAIRPTSVLRKPSELKAVLGRDQMRLYKLIWERFVASQMAPAVLDTMSVDLTNNGLT
FRANGSKVKFSGFMKVYVEGKDDQMEEKDRMLPDLQEGDTVLSKDIEPEQHFTQPPPRYTEARLVKTLEERGIGRPSTYA
PTLDTIQRRGYVALDNKRFVPTELGQIVLDLIMEFFPEIINVEFTAKMERDLDHVEEGNTEWVKIIDNFYTDFEKRVKKA
ESEMKEVEIEPEYAGEDCELCSSPMVYKMGRYGKFLACSNFPDCRNTKPIVKQIGVKCPSCGEGNIVERKSKKKRVFYGC
DRYPDCEFVSWDKPIERKCPKCGKMLVEKKLKKGIQVQCVECDYKEEPQK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Homo sapiens, GI10835218, Length=624, Percent_Identity=25.8012820512821, Blast_Score=134, Evalue=4e-31,
Organism=Homo sapiens, GI4507635, Length=673, Percent_Identity=22.8826151560178, Blast_Score=107, Evalue=3e-23,
Organism=Escherichia coli, GI1787529, Length=752, Percent_Identity=40.1595744680851, Blast_Score=515, Evalue=1e-147,
Organism=Escherichia coli, GI48994931, Length=108, Percent_Identity=38.8888888888889, Blast_Score=102, Evalue=1e-22,
Organism=Escherichia coli, GI1788061, Length=518, Percent_Identity=22.5868725868726, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI32563869, Length=565, Percent_Identity=26.0176991150442, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17555378, Length=506, Percent_Identity=23.9130434782609, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323263, Length=562, Percent_Identity=23.8434163701068, Blast_Score=107, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24585251, Length=622, Percent_Identity=24.2765273311897, Blast_Score=134, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24640096, Length=543, Percent_Identity=25.2302025782689, Blast_Score=112, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOP1_BACSU (P39814)

Other databases:

- EMBL:   L27797
- EMBL:   AJ000975
- EMBL:   AL009126
- PIR:   G69724
- RefSeq:   NP_389494.1
- ProteinModelPortal:   P39814
- SMR:   P39814
- EnsemblBacteria:   EBBACT00000001698
- GeneID:   937982
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU16120
- NMPDR:   fig|224308.1.peg.1614
- GenoList:   BSU16120
- GeneTree:   EBGT00070000032133
- HOGENOM:   HBG512935
- OMA:   EEYWSID
- PhylomeDB:   P39814
- ProtClustDB:   PRK05582
- BioCyc:   BSUB:BSU16120-MONOMER
- BRENDA:   5.99.1.2
- GO:   GO:0005694
- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171
- Gene3D:   G3DSA:1.10.460.10
- Gene3D:   G3DSA:2.70.20.10
- PANTHER:   PTHR11390
- PRINTS:   PR00417
- SMART:   SM00437
- SMART:   SM00436
- SMART:   SM00493
- TIGRFAMs:   TIGR01051

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom; SSF56712 Topo_IA_core

EC number: =5.99.1.2

Molecular weight: Translated: 79079; Mature: 78948

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: ACT_SITE 298-298

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDYLVIVESPAKAKTIERYLGKKYKVKASMGHVRDLPKSQMGVDIEQNFEPKYITIRGK
CCCEEEEEECCCHHHHHHHHCCCCEEEEHHHHHHHCCCHHHHCCCHHCCCCCCEEEEECC
GPVLKELKTAAKKAKKVYLAADPDREGEAIAWHLAHSLDLDLNSDCRVVFNEITKDAIKE
CCHHHHHHHHHHHHHEEEEEECCCCCCCEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHH
SFKHPRMINMDLVDAQQARRILDRLVGYKISPILWKKVKKGLSAGRVQSVALRLIIDREK
HHCCCCEEEEHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCH
EINDFKPEEYWTIDGTFLKGQETFEASFFGKNGKKLPLNSEADVKEILSQLKGNQYTVEK
HCCCCCCCCEEEECCEEECCCHHHHHHEECCCCCCCCCCCCCCHHHHHHHHCCCCCHHHH
VTKKERKRNPALPFTTSTLQQEAARKLNFRAKKTMMIAQQLYEGIDLGREGTVGLITYMR
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEE
TDSTRISNTAVDEAAAFIDQTYGKEFLGGKRKPAKKNENAQDAHEAIRPTSVLRKPSELK
CCCCCCCHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHCCHHHHH
AVLGRDQMRLYKLIWERFVASQMAPAVLDTMSVDLTNNGLTFRANGSKVKFSGFMKVYVE
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEECCCEEEEEEEEEEEEE
GKDDQMEEKDRMLPDLQEGDTVLSKDIEPEQHFTQPPPRYTEARLVKTLEERGIGRPSTY
CCCCHHHHHHHCCCCCCCCCHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCC
APTLDTIQRRGYVALDNKRFVPTELGQIVLDLIMEFFPEIINVEFTAKMERDLDHVEEGN
CCCHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEHHHHHHHHHHHCCC
TEWVKIIDNFYTDFEKRVKKAESEMKEVEIEPEYAGEDCELCSSPMVYKMGRYGKFLACS
CHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHCCCCHHHHCCCCCEEEEC
NFPDCRNTKPIVKQIGVKCPSCGEGNIVERKSKKKRVFYGCDRYPDCEFVSWDKPIERKC
CCCCCCCCCHHHHHHCCCCCCCCCCCEECCCCCCCCEEECCCCCCCCCEECCCCCHHHHC
PKCGKMLVEKKLKKGIQVQCVECDYKEEPQK
CHHHHHHHHHHHHCCCEEEEEECCCCCCCCC
>Mature Secondary Structure 
SDYLVIVESPAKAKTIERYLGKKYKVKASMGHVRDLPKSQMGVDIEQNFEPKYITIRGK
CCEEEEEECCCHHHHHHHHCCCCEEEEHHHHHHHCCCHHHHCCCHHCCCCCCEEEEECC
GPVLKELKTAAKKAKKVYLAADPDREGEAIAWHLAHSLDLDLNSDCRVVFNEITKDAIKE
CCHHHHHHHHHHHHHEEEEEECCCCCCCEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHH
SFKHPRMINMDLVDAQQARRILDRLVGYKISPILWKKVKKGLSAGRVQSVALRLIIDREK
HHCCCCEEEEHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCH
EINDFKPEEYWTIDGTFLKGQETFEASFFGKNGKKLPLNSEADVKEILSQLKGNQYTVEK
HCCCCCCCCEEEECCEEECCCHHHHHHEECCCCCCCCCCCCCCHHHHHHHHCCCCCHHHH
VTKKERKRNPALPFTTSTLQQEAARKLNFRAKKTMMIAQQLYEGIDLGREGTVGLITYMR
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEE
TDSTRISNTAVDEAAAFIDQTYGKEFLGGKRKPAKKNENAQDAHEAIRPTSVLRKPSELK
CCCCCCCHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHCCHHHHH
AVLGRDQMRLYKLIWERFVASQMAPAVLDTMSVDLTNNGLTFRANGSKVKFSGFMKVYVE
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEECCCEEEEEEEEEEEEE
GKDDQMEEKDRMLPDLQEGDTVLSKDIEPEQHFTQPPPRYTEARLVKTLEERGIGRPSTY
CCCCHHHHHHHCCCCCCCCCHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCC
APTLDTIQRRGYVALDNKRFVPTELGQIVLDLIMEFFPEIINVEFTAKMERDLDHVEEGN
CCCHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEHHHHHHHHHHHCCC
TEWVKIIDNFYTDFEKRVKKAESEMKEVEIEPEYAGEDCELCSSPMVYKMGRYGKFLACS
CHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHCCCCHHHHCCCCCEEEEC
NFPDCRNTKPIVKQIGVKCPSCGEGNIVERKSKKKRVFYGCDRYPDCEFVSWDKPIERKC
CCCCCCCCCHHHHHHCCCCCCCCCCCEECCCCCCCCEEECCCCCCCCCEECCCCCHHHHC
PKCGKMLVEKKLKKGIQVQCVECDYKEEPQK
CHHHHHHHHHHHHCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377