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The map label for this gene is gid

Identifier: 16078676

GI number: 16078676

Start: 1685812

End: 1687119

Strand: Direct

Name: gid

Synonym: BSU16130

Alternate gene names: 16078676

Gene position: 1685812-1687119 (Clockwise)

Preceding gene: 16078675

Following gene: 16078677

Centisome position: 39.99

GC content: 43.12

Gene sequence:

>1308_bases
ATGAACCAACAAACAGTGAATGTAATCGGAGCCGGACTCGCAGGAAGTGAAGCAGCATGGCAGCTTGCCAAACGTGGGAT
TCAAGTCAAATTGTATGAAATGCGGCCAGTTAAACAAACGCCTGCGCATCATACAGATAAATTTGCTGAGCTTGTCTGCA
GCAACTCTCTTCGCTCTAATACACTTGCTAACGCTGTCGGTGTATTAAAGGAAGAAATGCGTGCGCTTGATTCAGCCATT
ATCGCTGCGGCTGACGAATGTTCGGTGCCTGCCGGGGGCGCTCTTGCAGTAGACCGTCATGAATTTGCCGCAAGTGTGAC
AAATCGGGTGAAAAATCATCCAAACGTAACCGTTATTAATGAAGAAGTGACTGAAATTCCTGAAGGCCCTACTATCATCG
CAACGGGTCCGTTAACATCTGAATCGCTGTCTGCCCAGCTGAAGGAGCTGACTGGAGAGGACTATTTATATTTTTATGAC
GCAGCGGCGCCAATTGTAGAAAAAGACAGCCTTGATATGGATAAAGTGTACCTGAAATCCCGTTATGATAAAGGTGAAGC
AGCATATTTGAACTGCCCGATGACAGAAGAAGAGTTTGACCGTTTTCATGAAGCATTAACATCAGCAGAAACAGTGCCGT
TAAAAGAATTTGAAAAAGAGATTTTCTTTGAAGGCTGCATGCCGATTGAAGTCATGGCAAAACGGGGTAAGAAAACAATG
CTTTTCGGTCCGATGAAACCAGTTGGTCTAGAGCATCCTGTTACAGGAAAACGCCCTTATGCCGTCGTTCAGCTCAGACA
GGATGATGCTGCGGGAACACTTTATAATATTGTAGGATTCCAGACACATTTAAAATGGGGAGACCAAAAGGAAGTTCTCA
AGTTGATTCCAGGACTTGAAAATGTAGAAATCGTCAGATATGGCGTGATGCATAGAAACACATTTATTAACTCTCCAAGC
CTGTTAAAGCCGACTTATCAATTTAAAAACCGCAGTGATCTGTTCTTTGCAGGCCAAATGACGGGAGTAGAAGGATATGT
GGAATCAGCTGCCTCAGGACTTGTAGCAGGCATTAATGCGGCGAAGCTTGTATTGGGAGAAGAGCTTGTGATCTTCCCTC
AGGAAACAGCAATTGGCAGTATGGCACATTATATTACAACAACAAACCAGAAGAACTTCCAGCCGATGAATGCAAACTTT
GGGCTTTTGAAAGAATTGCCAGTTAAAATTAAAAATAAAAAAGAACGAAATGAACAATACGCGAACCGTGCGATTGAAAC
AATTCAAACAATTTCGAAAACAATATAG

Upstream 100 bases:

>100_bases
TTATAAGGAAGAACCACAGAAGTAGCGGTGAGCAGGGTCTTGCTCACCTTCTTTGTGTGTTAAAAGAAGGCCTTGAATAA
TAAACTAGGAGATGTGAAAG

Downstream 100 bases:

>100_bases
GTATTGATTGCAACCTGAGCGCGATTTGTGATACCATTTAAAAGCCCTTCCTGGGGAGGTATTAGGCATGGAGAATGTTA
AGAATTTCGTAAAGTTATTC

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MNQQTVNVIGAGLAGSEAAWQLAKRGIQVKLYEMRPVKQTPAHHTDKFAELVCSNSLRSNTLANAVGVLKEEMRALDSAI
IAAADECSVPAGGALAVDRHEFAASVTNRVKNHPNVTVINEEVTEIPEGPTIIATGPLTSESLSAQLKELTGEDYLYFYD
AAAPIVEKDSLDMDKVYLKSRYDKGEAAYLNCPMTEEEFDRFHEALTSAETVPLKEFEKEIFFEGCMPIEVMAKRGKKTM
LFGPMKPVGLEHPVTGKRPYAVVQLRQDDAAGTLYNIVGFQTHLKWGDQKEVLKLIPGLENVEIVRYGVMHRNTFINSPS
LLKPTYQFKNRSDLFFAGQMTGVEGYVESAASGLVAGINAAKLVLGEELVIFPQETAIGSMAHYITTTNQKNFQPMNANF
GLLKELPVKIKNKKERNEQYANRAIETIQTISKTI

Sequences:

>Translated_435_residues
MNQQTVNVIGAGLAGSEAAWQLAKRGIQVKLYEMRPVKQTPAHHTDKFAELVCSNSLRSNTLANAVGVLKEEMRALDSAI
IAAADECSVPAGGALAVDRHEFAASVTNRVKNHPNVTVINEEVTEIPEGPTIIATGPLTSESLSAQLKELTGEDYLYFYD
AAAPIVEKDSLDMDKVYLKSRYDKGEAAYLNCPMTEEEFDRFHEALTSAETVPLKEFEKEIFFEGCMPIEVMAKRGKKTM
LFGPMKPVGLEHPVTGKRPYAVVQLRQDDAAGTLYNIVGFQTHLKWGDQKEVLKLIPGLENVEIVRYGVMHRNTFINSPS
LLKPTYQFKNRSDLFFAGQMTGVEGYVESAASGLVAGINAAKLVLGEELVIFPQETAIGSMAHYITTTNQKNFQPMNANF
GLLKELPVKIKNKKERNEQYANRAIETIQTISKTI
>Mature_435_residues
MNQQTVNVIGAGLAGSEAAWQLAKRGIQVKLYEMRPVKQTPAHHTDKFAELVCSNSLRSNTLANAVGVLKEEMRALDSAI
IAAADECSVPAGGALAVDRHEFAASVTNRVKNHPNVTVINEEVTEIPEGPTIIATGPLTSESLSAQLKELTGEDYLYFYD
AAAPIVEKDSLDMDKVYLKSRYDKGEAAYLNCPMTEEEFDRFHEALTSAETVPLKEFEKEIFFEGCMPIEVMAKRGKKTM
LFGPMKPVGLEHPVTGKRPYAVVQLRQDDAAGTLYNIVGFQTHLKWGDQKEVLKLIPGLENVEIVRYGVMHRNTFINSPS
LLKPTYQFKNRSDLFFAGQMTGVEGYVESAASGLVAGINAAKLVLGEELVIFPQETAIGSMAHYITTTNQKNFQPMNANF
GLLKELPVKIKNKKERNEQYANRAIETIQTISKTI

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily [H]

Homologues:

Organism=Escherichia coli, GI2367273, Length=90, Percent_Identity=43.3333333333333, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17534255, Length=129, Percent_Identity=36.4341085271318, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321202, Length=97, Percent_Identity=42.2680412371134, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: =2.1.1.74 [H]

Molecular weight: Translated: 48064; Mature: 48064

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQQTVNVIGAGLAGSEAAWQLAKRGIQVKLYEMRPVKQTPAHHTDKFAELVCSNSLRSN
CCCCEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHH
TLANAVGVLKEEMRALDSAIIAAADECSVPAGGALAVDRHEFAASVTNRVKNHPNVTVIN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECHHHHHHHHHHHHCCCCCEEEEC
EEVTEIPEGPTIIATGPLTSESLSAQLKELTGEDYLYFYDAAAPIVEKDSLDMDKVYLKS
CHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHH
RYDKGEAAYLNCPMTEEEFDRFHEALTSAETVPLKEFEKEIFFEGCMPIEVMAKRGKKTM
CCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHCCCEEE
LFGPMKPVGLEHPVTGKRPYAVVQLRQDDAAGTLYNIVGFQTHLKWGDQKEVLKLIPGLE
EECCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCC
NVEIVRYGVMHRNTFINSPSLLKPTYQFKNRSDLFFAGQMTGVEGYVESAASGLVAGINA
CCEEEEEEHHHCCCCCCCCCCCCCHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCH
AKLVLGEELVIFPQETAIGSMAHYITTTNQKNFQPMNANFGLLKELPVKIKNKKERNEQY
HHEEECCEEEEEECHHHHHHHHHEEEECCCCCCCCCCCCCCCHHHCCCHHCCCHHHHHHH
ANRAIETIQTISKTI
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MNQQTVNVIGAGLAGSEAAWQLAKRGIQVKLYEMRPVKQTPAHHTDKFAELVCSNSLRSN
CCCCEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHH
TLANAVGVLKEEMRALDSAIIAAADECSVPAGGALAVDRHEFAASVTNRVKNHPNVTVIN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECHHHHHHHHHHHHCCCCCEEEEC
EEVTEIPEGPTIIATGPLTSESLSAQLKELTGEDYLYFYDAAAPIVEKDSLDMDKVYLKS
CHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHH
RYDKGEAAYLNCPMTEEEFDRFHEALTSAETVPLKEFEKEIFFEGCMPIEVMAKRGKKTM
CCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHCCCEEE
LFGPMKPVGLEHPVTGKRPYAVVQLRQDDAAGTLYNIVGFQTHLKWGDQKEVLKLIPGLE
EECCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCC
NVEIVRYGVMHRNTFINSPSLLKPTYQFKNRSDLFFAGQMTGVEGYVESAASGLVAGINA
CCEEEEEEHHHCCCCCCCCCCCCCHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCH
AKLVLGEELVIFPQETAIGSMAHYITTTNQKNFQPMNANFGLLKELPVKIKNKKERNEQY
HHEEECCEEEEEECHHHHHHHHHEEEECCCCCCCCCCCCCCCHHHCCCHHCCCHHHHHHH
ANRAIETIQTISKTI
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA