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The map label for this gene is trmD

Identifier: 16078666

GI number: 16078666

Start: 1675171

End: 1675902

Strand: Direct

Name: trmD

Synonym: BSU16030

Alternate gene names: 16078666

Gene position: 1675171-1675902 (Clockwise)

Preceding gene: 16078665

Following gene: 16078667

Centisome position: 39.74

GC content: 46.99

Gene sequence:

>732_bases
ATGAAAATCGATTTTTTGACGCTGTTTCCCGAAATGTTTGAAGGCGTGCTCGGCTCATCGATTCTTCAAAAAGCCCAGGA
AAAAGATGCGGTGCAGTTTCAAGTCGTAAATTTCCGAGAGTACTCTGATAACAAGCACAATACTGTTGATGATTATCCTT
ATGGCGGCGGGGCAGGCATGGTTCTCAAGCCCCAGCCTGTTTTTGACGCGGTCGAGGACCTGACATCAAAGGCAGCCGCT
GCTCCGCGTATTATCCTCGTATGCCCGCAAGGTGAGCGTTTTACCCAAAAAAAAGCCGAACAATTAGCTAAGGAAGAGCA
TTTGCTGTTCATTTGCGGCCACTATGAAGGCTATGATGAACGCATTCGCGAGCACTTGGTAACGGATGAAATATCAATTG
GCGACTTTGTTCTGACGGGCGGTGAGCTCCCTGCAATGATGATCGCAGACAGTGTGGTCAGACTGCTTCCGGGTGTACTG
GGTAAAGAGGCTTCCCATATTGAGGATTCCTTCAGCACCGGACTTTTAGAGCACCCGCATTATACAAGACCGGCAGATTA
CAAAGGTTTAAAAGTGCCTGAAACACTCTTGTCAGGAAACCATGCAAAAATTGAAGAATGGCGGAATAAAGAATCGATCA
GAAGGACCTACCTAAGACGTCCGGATCTGTTGAAAGACCACCCGCTTACAGAGCAGCAAAGAAAGTGGATTTCTGAATGG
GAAAAAGAATAG

Upstream 100 bases:

>100_bases
AAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTTAAACATATCGATGTCAGGGAAAAGAAAATTGAGATTGAACTC
ATGGAAGGGTTAATAGACGA

Downstream 100 bases:

>100_bases
ATTTTATTGCATAGGCTATGAAATTGTGATAAGATACTACTTGTGGCTTAAGCGGGCTTATCCGCTGCAAGCTTGAAAAC
GATGTTCCGCTGTGCCGGTT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MKIDFLTLFPEMFEGVLGSSILQKAQEKDAVQFQVVNFREYSDNKHNTVDDYPYGGGAGMVLKPQPVFDAVEDLTSKAAA
APRIILVCPQGERFTQKKAEQLAKEEHLLFICGHYEGYDERIREHLVTDEISIGDFVLTGGELPAMMIADSVVRLLPGVL
GKEASHIEDSFSTGLLEHPHYTRPADYKGLKVPETLLSGNHAKIEEWRNKESIRRTYLRRPDLLKDHPLTEQQRKWISEW
EKE

Sequences:

>Translated_243_residues
MKIDFLTLFPEMFEGVLGSSILQKAQEKDAVQFQVVNFREYSDNKHNTVDDYPYGGGAGMVLKPQPVFDAVEDLTSKAAA
APRIILVCPQGERFTQKKAEQLAKEEHLLFICGHYEGYDERIREHLVTDEISIGDFVLTGGELPAMMIADSVVRLLPGVL
GKEASHIEDSFSTGLLEHPHYTRPADYKGLKVPETLLSGNHAKIEEWRNKESIRRTYLRRPDLLKDHPLTEQQRKWISEW
EKE
>Mature_243_residues
MKIDFLTLFPEMFEGVLGSSILQKAQEKDAVQFQVVNFREYSDNKHNTVDDYPYGGGAGMVLKPQPVFDAVEDLTSKAAA
APRIILVCPQGERFTQKKAEQLAKEEHLLFICGHYEGYDERIREHLVTDEISIGDFVLTGGELPAMMIADSVVRLLPGVL
GKEASHIEDSFSTGLLEHPHYTRPADYKGLKVPETLLSGNHAKIEEWRNKESIRRTYLRRPDLLKDHPLTEQQRKWISEW
EKE

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=243, Percent_Identity=46.0905349794239, Blast_Score=232, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_BACSU (O31741)

Other databases:

- EMBL:   AL009126
- PIR:   F69725
- RefSeq:   NP_389485.1
- ProteinModelPortal:   O31741
- SMR:   O31741
- EnsemblBacteria:   EBBACT00000001559
- GeneID:   936650
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU16030
- NMPDR:   fig|224308.1.peg.1605
- GenoList:   BSU16030
- GeneTree:   EBGT00050000001149
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- PhylomeDB:   O31741
- ProtClustDB:   PRK00026
- BioCyc:   BSUB:BSU16030-MONOMER
- BRENDA:   2.1.1.31
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 27709; Mature: 27709

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDFLTLFPEMFEGVLGSSILQKAQEKDAVQFQVVNFREYSDNKHNTVDDYPYGGGAGM
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHCCCCCCCCCCCCCCCCCCC
VLKPQPVFDAVEDLTSKAAAAPRIILVCPQGERFTQKKAEQLAKEEHLLFICGHYEGYDE
EECCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCEEEEEECCCCHHH
RIREHLVTDEISIGDFVLTGGELPAMMIADSVVRLLPGVLGKEASHIEDSFSTGLLEHPH
HHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCC
YTRPADYKGLKVPETLLSGNHAKIEEWRNKESIRRTYLRRPDLLKDHPLTEQQRKWISEW
CCCCCCCCCCCCCHHHHCCCCHHHHHHHCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
EKE
CCC
>Mature Secondary Structure
MKIDFLTLFPEMFEGVLGSSILQKAQEKDAVQFQVVNFREYSDNKHNTVDDYPYGGGAGM
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHCCCCCCCCCCCCCCCCCCC
VLKPQPVFDAVEDLTSKAAAAPRIILVCPQGERFTQKKAEQLAKEEHLLFICGHYEGYDE
EECCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCEEEEEECCCCHHH
RIREHLVTDEISIGDFVLTGGELPAMMIADSVVRLLPGVLGKEASHIEDSFSTGLLEHPH
HHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCC
YTRPADYKGLKVPETLLSGNHAKIEEWRNKESIRRTYLRRPDLLKDHPLTEQQRKWISEW
CCCCCCCCCCCCCHHHHCCCCHHHHHHHCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
EKE
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377