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The map label for this gene is pyrE

Identifier: 16078620

GI number: 16078620

Start: 1629320

End: 1629970

Strand: Direct

Name: pyrE

Synonym: BSU15560

Alternate gene names: 16078620

Gene position: 1629320-1629970 (Clockwise)

Preceding gene: 16078619

Following gene: 16078621

Centisome position: 38.65

GC content: 45.78

Gene sequence:

>651_bases
ATGGGAGGGAATCAAATCTTGAAACAAATCATCGCAAAACATCTATTAGACATCCAAGCTGTATTTTTACGCCCGAACGA
GCCGTTCACATGGGCAAGCGGCATTTTATCACCGATCTACTGTGACAACCGCCTTACGCTATCTTTCCCAGAGGTCAGAA
ACGATGTTGCTTCAGGTATCAGCAAGCTTGTTAAAGAGCATTTTCCTGAAGCTGAAATGATTGCGGGAACAGCAACTGCC
GGTATTCCTCATGCTGCTCTTGCGGCGGACCATTTGAATCTTCCGATGTGTTATGTGAGGAGCAAGCCGAAGGCGCACGG
AAAAGGCAATCAGATTGAGGGAGCTGTGCAAGAAGGGCAAAAAACAGTCGTCATTGAAGACTTAATTTCCACAGGAGGCA
GCGTGCTTGAAGCTTGTGCAGCTTTACAAGCGGCCGGCTGTGAAGTGCTTGGTGTCGTCTCAATCTTTACGTACGGACTT
CCTAAAGCGGAGGAAGCCTTCGCAAAGGCAGAACTGCCATACTACTCATTAACCGATTATGATACGCTCACAGAGGTCGC
GCTTGAAAACGGAAATATTCATTCAGATGATCTAAAAAAGCTGCAAACATGGAAACGAAATCCCGAGTCAAAAGATTGGT
TTAAAAAATAA

Upstream 100 bases:

>100_bases
CTGCCATTGCAAGAGAGAAAGGTTCATCAGCGATTGTAGTAGGACGCTCGATTACAAAAGCGGAAGACCCGGTAAAAGCC
TATAAGGCTGTCAGACTTGA

Downstream 100 bases:

>100_bases
AAAATAAATTCAAATGATGTAAAGAGGCTTCAAAGCCTTGCTGTACTTGAAAACAGGCTGTGAGGCCTGTTTTTTTATTA
ATCCTATAAAAAAAGTCGGG

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MGGNQILKQIIAKHLLDIQAVFLRPNEPFTWASGILSPIYCDNRLTLSFPEVRNDVASGISKLVKEHFPEAEMIAGTATA
GIPHAALAADHLNLPMCYVRSKPKAHGKGNQIEGAVQEGQKTVVIEDLISTGGSVLEACAALQAAGCEVLGVVSIFTYGL
PKAEEAFAKAELPYYSLTDYDTLTEVALENGNIHSDDLKKLQTWKRNPESKDWFKK

Sequences:

>Translated_216_residues
MGGNQILKQIIAKHLLDIQAVFLRPNEPFTWASGILSPIYCDNRLTLSFPEVRNDVASGISKLVKEHFPEAEMIAGTATA
GIPHAALAADHLNLPMCYVRSKPKAHGKGNQIEGAVQEGQKTVVIEDLISTGGSVLEACAALQAAGCEVLGVVSIFTYGL
PKAEEAFAKAELPYYSLTDYDTLTEVALENGNIHSDDLKKLQTWKRNPESKDWFKK
>Mature_215_residues
GGNQILKQIIAKHLLDIQAVFLRPNEPFTWASGILSPIYCDNRLTLSFPEVRNDVASGISKLVKEHFPEAEMIAGTATAG
IPHAALAADHLNLPMCYVRSKPKAHGKGNQIEGAVQEGQKTVVIEDLISTGGSVLEACAALQAAGCEVLGVVSIFTYGLP
KAEEAFAKAELPYYSLTDYDTLTEVALENGNIHSDDLKKLQTWKRNPESKDWFKK

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=121, Percent_Identity=29.7520661157025, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17508631, Length=128, Percent_Identity=29.6875, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17554964, Length=166, Percent_Identity=28.9156626506024, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI17933654, Length=118, Percent_Identity=28.8135593220339, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_BACSU (P25972)

Other databases:

- EMBL:   M59757
- EMBL:   AL009126
- EMBL:   AJ000974
- PIR:   F69686
- RefSeq:   NP_389439.1
- ProteinModelPortal:   P25972
- SMR:   P25972
- EnsemblBacteria:   EBBACT00000002919
- GeneID:   936714
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU15560
- NMPDR:   fig|224308.1.peg.1558
- GenoList:   BSU15560
- GeneTree:   EBGT00050000002725
- HOGENOM:   HBG404341
- OMA:   LPMTYVR
- ProtClustDB:   PRK00455
- BioCyc:   BSUB:BSU15560-MONOMER
- BRENDA:   2.4.2.10
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 23522; Mature: 23391

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 100-100 BINDING 104-104 BINDING 106-106 BINDING 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGNQILKQIIAKHLLDIQAVFLRPNEPFTWASGILSPIYCDNRLTLSFPEVRNDVASGI
CCHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHCCCHHEECCEEEEECHHHHHHHHHHH
SKLVKEHFPEAEMIAGTATAGIPHAALAADHLNLPMCYVRSKPKAHGKGNQIEGAVQEGQ
HHHHHHHCCCHHHHHCCHHCCCCHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHCCC
KTVVIEDLISTGGSVLEACAALQAAGCEVLGVVSIFTYGLPKAEEAFAKAELPYYSLTDY
CEEHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCEECCCH
DTLTEVALENGNIHSDDLKKLQTWKRNPESKDWFKK
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHCCC
>Mature Secondary Structure 
GGNQILKQIIAKHLLDIQAVFLRPNEPFTWASGILSPIYCDNRLTLSFPEVRNDVASGI
CHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHCCCHHEECCEEEEECHHHHHHHHHHH
SKLVKEHFPEAEMIAGTATAGIPHAALAADHLNLPMCYVRSKPKAHGKGNQIEGAVQEGQ
HHHHHHHCCCHHHHHCCHHCCCCHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHCCC
KTVVIEDLISTGGSVLEACAALQAAGCEVLGVVSIFTYGLPKAEEAFAKAELPYYSLTDY
CEEHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCEECCCH
DTLTEVALENGNIHSDDLKKLQTWKRNPESKDWFKK
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1709162; 9384377; 9006060