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The map label for this gene is cysH

Identifier: 16078621

GI number: 16078621

Start: 1630382

End: 1631083

Strand: Direct

Name: cysH

Synonym: BSU15570

Alternate gene names: 16078621

Gene position: 1630382-1631083 (Clockwise)

Preceding gene: 16078620

Following gene: 16078622

Centisome position: 38.67

GC content: 43.45

Gene sequence:

>702_bases
ATGTTAACGTATGATAATTGGGAAGAACCAACGATTACATTTCCGGAAGACGATCCTTATAAAGGAGCGCTGTCAGTTTT
AAAATGGGCATACGGCCATTACGGCGATCAGCTTGTTTATGCCTGCAGTTTCGGAATTGAGGGAATCGTTCTGATTGACT
TAATTTATAAAGTGAAAAAAGACGCAGAGATTGTGTTTCTTGATACAGGACTTCATTTTAAAGAAACCTATGAAACAATT
GAACGAGTGAAAGAGCGTTATCCGGGTCTTAATATCATCCTGAAAAAACCAGATCTCACCCTTGAGGAACAAGCCGAAGA
ACATGGTGACAAACTATGGGAAAGAGAGCCGAATCAGTGCTGCTATCTTAGAAAGGTCGTTCCTTTAAGAGAGGCGCTTT
CAGGACATCCAGCCTGGCTTTCCGGCCTGCGCCGTGATCAAGGGCCAAGCCGTGCCAATACGAATTTCTTAAACAAAGAT
GAAAAATTCAAGTCAGTAAAAGTTTGCCCGCTTATCCATTGGACTTGGAAAGACATTTGGAGATATACATCCCGGAATGA
ACTGGACTACAATCCGCTTCATGACCAAGGCTATCCAAGTATTGGCTGTGCGCCTTGCACTTCTCCCGCTTTTACAGCAG
AAGATTTGCGCTCAGGCAGATGGAATGGTATGGCGAAAACAGAATGCGGACTGCATGAATAA

Upstream 100 bases:

>100_bases
AAGGCTATCTGTATATCAGCAGCCGCGAATCACATTACATGGGAAAAGACAACCGGCAGAAAGCTACTGTTTGTTTGTCT
CCGAAAGGAGGAAAGAAGAA

Downstream 100 bases:

>100_bases
GGAGCTGCAAAATGGAATTAGCCGCTATTTTATTTAGCTTGTTTTTCGCGATGAATATTGGTGCAAGCGGCGCTGCAGCT
TCGATGGGGGTTGCTTACGG

Product: (phospho)adenosine phosphosulfate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MLTYDNWEEPTITFPEDDPYKGALSVLKWAYGHYGDQLVYACSFGIEGIVLIDLIYKVKKDAEIVFLDTGLHFKETYETI
ERVKERYPGLNIILKKPDLTLEEQAEEHGDKLWEREPNQCCYLRKVVPLREALSGHPAWLSGLRRDQGPSRANTNFLNKD
EKFKSVKVCPLIHWTWKDIWRYTSRNELDYNPLHDQGYPSIGCAPCTSPAFTAEDLRSGRWNGMAKTECGLHE

Sequences:

>Translated_233_residues
MLTYDNWEEPTITFPEDDPYKGALSVLKWAYGHYGDQLVYACSFGIEGIVLIDLIYKVKKDAEIVFLDTGLHFKETYETI
ERVKERYPGLNIILKKPDLTLEEQAEEHGDKLWEREPNQCCYLRKVVPLREALSGHPAWLSGLRRDQGPSRANTNFLNKD
EKFKSVKVCPLIHWTWKDIWRYTSRNELDYNPLHDQGYPSIGCAPCTSPAFTAEDLRSGRWNGMAKTECGLHE
>Mature_233_residues
MLTYDNWEEPTITFPEDDPYKGALSVLKWAYGHYGDQLVYACSFGIEGIVLIDLIYKVKKDAEIVFLDTGLHFKETYETI
ERVKERYPGLNIILKKPDLTLEEQAEEHGDKLWEREPNQCCYLRKVVPLREALSGHPAWLSGLRRDQGPSRANTNFLNKD
EKFKSVKVCPLIHWTWKDIWRYTSRNELDYNPLHDQGYPSIGCAPCTSPAFTAEDLRSGRWNGMAKTECGLHE

Specific function: Reduction of activated sulfate into sulfite

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily

Homologues:

Organism=Escherichia coli, GI1789121, Length=215, Percent_Identity=33.0232558139535, Blast_Score=98, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6325425, Length=207, Percent_Identity=36.231884057971, Blast_Score=125, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSH1_BACSU (P94498)

Other databases:

- EMBL:   U76751
- EMBL:   AJ000974
- EMBL:   AL009126
- PIR:   H69611
- RefSeq:   NP_389440.1
- ProteinModelPortal:   P94498
- SMR:   P94498
- EnsemblBacteria:   EBBACT00000003420
- GeneID:   938645
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU15570
- NMPDR:   fig|224308.1.peg.1559
- GenoList:   BSU15570
- GeneTree:   EBGT00050000002870
- HOGENOM:   HBG758022
- OMA:   KVEPAHR
- PhylomeDB:   P94498
- ProtClustDB:   CLSK887282
- BioCyc:   BSUB:BSU15570-MONOMER
- BRENDA:   1.8.4.8
- GO:   GO:0005737
- HAMAP:   MF_00063
- InterPro:   IPR011798
- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR02055
- TIGRFAMs:   TIGR00434

Pfam domain/function: PF01507 PAPS_reduct

EC number: =1.8.4.8

Molecular weight: Translated: 26975; Mature: 26975

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTYDNWEEPTITFPEDDPYKGALSVLKWAYGHYGDQLVYACSFGIEGIVLIDLIYKVKK
CCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCC
DAEIVFLDTGLHFKETYETIERVKERYPGLNIILKKPDLTLEEQAEEHGDKLWEREPNQC
CCEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCH
CYLRKVVPLREALSGHPAWLSGLRRDQGPSRANTNFLNKDEKFKSVKVCPLIHWTWKDIW
HHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHCCEEECEEEEHHHHHHH
RYTSRNELDYNPLHDQGYPSIGCAPCTSPAFTAEDLRSGRWNGMAKTECGLHE
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLTYDNWEEPTITFPEDDPYKGALSVLKWAYGHYGDQLVYACSFGIEGIVLIDLIYKVKK
CCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCC
DAEIVFLDTGLHFKETYETIERVKERYPGLNIILKKPDLTLEEQAEEHGDKLWEREPNQC
CCEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCH
CYLRKVVPLREALSGHPAWLSGLRRDQGPSRANTNFLNKDEKFKSVKVCPLIHWTWKDIW
HHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHCCEEECEEEEHHHHHHH
RYTSRNELDYNPLHDQGYPSIGCAPCTSPAFTAEDLRSGRWNGMAKTECGLHE
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9006060; 9384377