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The map label for this gene is pdhD

Identifier: 16078525

GI number: 16078525

Start: 1531870

End: 1533282

Strand: Direct

Name: pdhD

Synonym: BSU14610

Alternate gene names: 16078525

Gene position: 1531870-1533282 (Clockwise)

Preceding gene: 16078524

Following gene: 255767338

Centisome position: 36.34

GC content: 44.52

Gene sequence:

>1413_bases
ATGGTAGTAGGAGATTTCCCTATTGAAACAGATACTCTTGTAATTGGTGCGGGACCTGGCGGCTATGTAGCTGCCATCCG
CGCTGCACAGCTTGGACAAAAAGTAACAGTCGTTGAAAAAGCAACTCTTGGAGGCGTTTGTCTGAACGTTGGATGTATCC
CTTCAAAAGCGCTGATCAATGCAGGTCACCGTTATGAGAATGCAAAACATTCTGATGACATGGGAATCACTGCTGAGAAT
GTAACAGTTGATTTCACAAAAGTTCAAGAATGGAAAGCTTCTGTTGTCAACAAGCTTACTGGCGGTGTAGCAGGTCTTCT
TAAAGGCAACAAAGTAGATGTTGTAAAAGGTGAAGCTTACTTTGTAGACAGCAATTCAGTTCGTGTTATGGATGAGAACT
CTGCTCAAACATACACGTTTAAAAACGCAATCATTGCTACTGGTTCTCGTCCTATCGAATTGCCAAACTTCAAATATAGT
GAGCGTGTCCTGAATTCAACTGGCGCTTTGGCTCTTAAAGAAATTCCTAAAAAGCTCGTTGTTATCGGCGGCGGATACAT
CGGAACTGAACTTGGAACTGCGTATGCTAACTTCGGTACTGAACTTGTTATTCTTGAAGGCGGAGATGAAATTCTTCCTG
GCTTCGAAAAACAAATGAGTTCTCTCGTTACACGCAGACTGAAGAAAAAAGGCAACGTTGAAATCCATACAAACGCGATG
GCTAAAGGCGTTGAAGAAAGACCAGACGGCGTAACAGTTACTTTCGAAGTAAAAGGCGAAGAAAAAACTGTTGATGCTGA
TTACGTATTGATTACAGTAGGACGCCGTCCAAACACTGATGAGCTTGGTCTTGAGCAAGTCGGTATCGAAATGACGGACC
GCGGTATCGTGAAAACTGACAAACAGTGCCGCACAAACGTACCTAACATTTATGCAATCGGTGATATCATCGAAGGACCG
CCGCTTGCTCATAAAGCATCTTACGAAGGTAAAATCGCTGCAGAAGCTATCGCTGGAGAGCCTGCAGAAATCGATTACCT
TGGTATTCCTGCGGTTGTTTTCTCTGAGCCTGAACTTGCATCAGTTGGTTACACTGAAGCACAGGCGAAAGAAGAAGGTC
TTGACATTGTTGCTGCTAAATTCCCATTTGCAGCAAACGGCCGCGCGCTTTCTCTTAACGAAACAGACGGCTTCATGAAG
CTGATCACTCGTAAAGAGGACGGTCTTGTGATCGGTGCGCAAATCGCCGGAGCAAGTGCTTCTGATATGATTTCTGAATT
AAGCTTAGCGATTGAAGGCGGCATGACTGCTGAAGATATCGCAATGACAATTCACGCTCACCCAACATTGGGCGAAATCA
CAATGGAAGCTGCTGAAGTGGCAATCGGAAGTCCGATTCACATCGTAAAATAA

Upstream 100 bases:

>100_bases
GACCACCGTATGATCGACGGAGCAACTGCGCAAAATGCATTAAATCACATCAAGCGTTTACTGAACGATCCACAATTAAT
TTTAATGGAGGCGTAATGTT

Downstream 100 bases:

>100_bases
TTTTCATATCAAAAACAGCCCCGCTTTGAGCGAGGGCTGTTTTTTTATTTTGACAGCACATGCTGTAATGGTTTAATGAT
GTCTTCTTTCTTTTTCACAC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; S complex, 50 kDa subunit

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MVVGDFPIETDTLVIGAGPGGYVAAIRAAQLGQKVTVVEKATLGGVCLNVGCIPSKALINAGHRYENAKHSDDMGITAEN
VTVDFTKVQEWKASVVNKLTGGVAGLLKGNKVDVVKGEAYFVDSNSVRVMDENSAQTYTFKNAIIATGSRPIELPNFKYS
ERVLNSTGALALKEIPKKLVVIGGGYIGTELGTAYANFGTELVILEGGDEILPGFEKQMSSLVTRRLKKKGNVEIHTNAM
AKGVEERPDGVTVTFEVKGEEKTVDADYVLITVGRRPNTDELGLEQVGIEMTDRGIVKTDKQCRTNVPNIYAIGDIIEGP
PLAHKASYEGKIAAEAIAGEPAEIDYLGIPAVVFSEPELASVGYTEAQAKEEGLDIVAAKFPFAANGRALSLNETDGFMK
LITRKEDGLVIGAQIAGASASDMISELSLAIEGGMTAEDIAMTIHAHPTLGEITMEAAEVAIGSPIHIVK

Sequences:

>Translated_470_residues
MVVGDFPIETDTLVIGAGPGGYVAAIRAAQLGQKVTVVEKATLGGVCLNVGCIPSKALINAGHRYENAKHSDDMGITAEN
VTVDFTKVQEWKASVVNKLTGGVAGLLKGNKVDVVKGEAYFVDSNSVRVMDENSAQTYTFKNAIIATGSRPIELPNFKYS
ERVLNSTGALALKEIPKKLVVIGGGYIGTELGTAYANFGTELVILEGGDEILPGFEKQMSSLVTRRLKKKGNVEIHTNAM
AKGVEERPDGVTVTFEVKGEEKTVDADYVLITVGRRPNTDELGLEQVGIEMTDRGIVKTDKQCRTNVPNIYAIGDIIEGP
PLAHKASYEGKIAAEAIAGEPAEIDYLGIPAVVFSEPELASVGYTEAQAKEEGLDIVAAKFPFAANGRALSLNETDGFMK
LITRKEDGLVIGAQIAGASASDMISELSLAIEGGMTAEDIAMTIHAHPTLGEITMEAAEVAIGSPIHIVK
>Mature_470_residues
MVVGDFPIETDTLVIGAGPGGYVAAIRAAQLGQKVTVVEKATLGGVCLNVGCIPSKALINAGHRYENAKHSDDMGITAEN
VTVDFTKVQEWKASVVNKLTGGVAGLLKGNKVDVVKGEAYFVDSNSVRVMDENSAQTYTFKNAIIATGSRPIELPNFKYS
ERVLNSTGALALKEIPKKLVVIGGGYIGTELGTAYANFGTELVILEGGDEILPGFEKQMSSLVTRRLKKKGNVEIHTNAM
AKGVEERPDGVTVTFEVKGEEKTVDADYVLITVGRRPNTDELGLEQVGIEMTDRGIVKTDKQCRTNVPNIYAIGDIIEGP
PLAHKASYEGKIAAEAIAGEPAEIDYLGIPAVVFSEPELASVGYTEAQAKEEGLDIVAAKFPFAANGRALSLNETDGFMK
LITRKEDGLVIGAQIAGASASDMISELSLAIEGGMTAEDIAMTIHAHPTLGEITMEAAEVAIGSPIHIVK

Specific function: Catalyzes the oxidation of dihydrolipoamide to lipoamide

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family

Homologues:

Organism=Homo sapiens, GI91199540, Length=466, Percent_Identity=43.7768240343348, Blast_Score=357, Evalue=1e-98,
Organism=Homo sapiens, GI50301238, Length=458, Percent_Identity=29.6943231441048, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI22035672, Length=455, Percent_Identity=30.3296703296703, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI291045266, Length=460, Percent_Identity=28.2608695652174, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI33519430, Length=454, Percent_Identity=26.2114537444934, Blast_Score=142, Evalue=8e-34,
Organism=Homo sapiens, GI33519428, Length=454, Percent_Identity=26.2114537444934, Blast_Score=142, Evalue=8e-34,
Organism=Homo sapiens, GI33519426, Length=454, Percent_Identity=26.2114537444934, Blast_Score=142, Evalue=8e-34,
Organism=Homo sapiens, GI148277065, Length=454, Percent_Identity=26.2114537444934, Blast_Score=142, Evalue=1e-33,
Organism=Homo sapiens, GI148277071, Length=456, Percent_Identity=26.3157894736842, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI291045268, Length=452, Percent_Identity=26.3274336283186, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1786307, Length=462, Percent_Identity=47.4025974025974, Blast_Score=400, Evalue=1e-112,
Organism=Escherichia coli, GI1789915, Length=440, Percent_Identity=30.6818181818182, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI87081717, Length=447, Percent_Identity=30.2013422818792, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI87082354, Length=463, Percent_Identity=29.5896328293737, Blast_Score=186, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI32565766, Length=461, Percent_Identity=43.8177874186551, Blast_Score=353, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI71983429, Length=455, Percent_Identity=31.2087912087912, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI71983419, Length=455, Percent_Identity=31.2087912087912, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17557007, Length=480, Percent_Identity=28.3333333333333, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71982272, Length=486, Percent_Identity=25.5144032921811, Blast_Score=108, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6321091, Length=471, Percent_Identity=39.2781316348195, Blast_Score=306, Evalue=7e-84,
Organism=Saccharomyces cerevisiae, GI6325166, Length=463, Percent_Identity=30.0215982721382, Blast_Score=192, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6325240, Length=478, Percent_Identity=27.1966527196653, Blast_Score=169, Evalue=1e-42,
Organism=Drosophila melanogaster, GI21358499, Length=470, Percent_Identity=45.531914893617, Blast_Score=359, Evalue=3e-99,
Organism=Drosophila melanogaster, GI24640553, Length=465, Percent_Identity=28.1720430107527, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24640549, Length=465, Percent_Identity=28.1720430107527, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24640551, Length=465, Percent_Identity=28.1720430107527, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI17737741, Length=468, Percent_Identity=26.0683760683761, Blast_Score=128, Evalue=1e-29,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): DLDH1_BACSU (P21880)

Other databases:

- EMBL:   M57435
- EMBL:   AF012285
- EMBL:   AL009126
- PIR:   E36718
- RefSeq:   NP_389344.1
- ProteinModelPortal:   P21880
- SMR:   P21880
- EnsemblBacteria:   EBBACT00000000385
- GeneID:   939492
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU14610
- NMPDR:   fig|224308.1.peg.1463
- GenoList:   BSU14610
- GeneTree:   EBGT00070000031801
- HOGENOM:   HBG515043
- OMA:   TLGEAIM
- PhylomeDB:   P21880
- ProtClustDB:   PRK06416
- BioCyc:   BSUB:BSU14610-MONOMER
- BioCyc:   MetaCyc:MONOMER-11687
- BRENDA:   1.8.1.4
- GO:   GO:0005737
- GO:   GO:0006096
- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327
- Gene3D:   G3DSA:3.30.390.30
- PANTHER:   PTHR22912:SF20
- PRINTS:   PR00368
- TIGRFAMs:   TIGR01350

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim; SSF55424 FAD/NAD-linked_reductase_dimer

EC number: =1.8.1.4

Molecular weight: Translated: 49733; Mature: 49733

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: ACT_SITE 447-447 BINDING 56-56 BINDING 119-119 BINDING 206-206 BINDING 315-315 BINDING 323-323

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVGDFPIETDTLVIGAGPGGYVAAIRAAQLGQKVTVVEKATLGGVCLNVGCIPSKALIN
CEECCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCCCHHHHC
AGHRYENAKHSDDMGITAENVTVDFTKVQEWKASVVNKLTGGVAGLLKGNKVDVVKGEAY
CCCCCCCCCCCCCCCEEECCEEEEEHHHHHHHHHHHHHHCCCEEEHCCCCEEEEEECCEE
FVDSNSVRVMDENSAQTYTFKNAIIATGSRPIELPNFKYSERVLNSTGALALKEIPKKLV
EEECCCEEEEECCCCEEEEECCEEEECCCCCEECCCCCHHHHHHCCCCCHHHHHCCCEEE
VIGGGYIGTELGTAYANFGTELVILEGGDEILPGFEKQMSSLVTRRLKKKGNVEIHTNAM
EEECCEECHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECHH
AKGVEERPDGVTVTFEVKGEEKTVDADYVLITVGRRPNTDELGLEQVGIEMTDRGIVKTD
HCCCCCCCCCEEEEEEECCCCEEECCCEEEEEECCCCCCCCCCHHHCCCEECCCCCEECC
KQCRTNVPNIYAIGDIIEGPPLAHKASYEGKIAAEAIAGEPAEIDYLGIPAVVFSEPELA
HHHHCCCCCEEEECCCCCCCCCCCCCCCCCCEEEHHHCCCCCEEEEECCCEEEECCCCCC
SVGYTEAQAKEEGLDIVAAKFPFAANGRALSLNETDGFMKLITRKEDGLVIGAQIAGASA
CCCCCHHHHHHCCCEEEEEECCCCCCCCEEEECCCCCHHEEEEECCCCEEEEEEECCCCH
SDMISELSLAIEGGMTAEDIAMTIHAHPTLGEITMEAAEVAIGSPIHIVK
HHHHHHHHHEECCCCCHHHEEEEEECCCCHHHHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure
MVVGDFPIETDTLVIGAGPGGYVAAIRAAQLGQKVTVVEKATLGGVCLNVGCIPSKALIN
CEECCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCCCHHHHC
AGHRYENAKHSDDMGITAENVTVDFTKVQEWKASVVNKLTGGVAGLLKGNKVDVVKGEAY
CCCCCCCCCCCCCCCEEECCEEEEEHHHHHHHHHHHHHHCCCEEEHCCCCEEEEEECCEE
FVDSNSVRVMDENSAQTYTFKNAIIATGSRPIELPNFKYSERVLNSTGALALKEIPKKLV
EEECCCEEEEECCCCEEEEECCEEEECCCCCEECCCCCHHHHHHCCCCCHHHHHCCCEEE
VIGGGYIGTELGTAYANFGTELVILEGGDEILPGFEKQMSSLVTRRLKKKGNVEIHTNAM
EEECCEECHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECHH
AKGVEERPDGVTVTFEVKGEEKTVDADYVLITVGRRPNTDELGLEQVGIEMTDRGIVKTD
HCCCCCCCCCEEEEEEECCCCEEECCCEEEEEECCCCCCCCCCHHHCCCEECCCCCEECC
KQCRTNVPNIYAIGDIIEGPPLAHKASYEGKIAAEAIAGEPAEIDYLGIPAVVFSEPELA
HHHHCCCCCEEEECCCCCCCCCCCCCCCCCCEEEHHHCCCCCEEEEECCCEEEECCCCCC
SVGYTEAQAKEEGLDIVAAKFPFAANGRALSLNETDGFMKLITRKEDGLVIGAQIAGASA
CCCCCHHHHHHCCCEEEEEECCCCCCCCEEEECCCCCHHEEEEECCCCEEEEEEECCCCH
SDMISELSLAIEGGMTAEDIAMTIHAHPTLGEITMEAAEVAIGSPIHIVK
HHHHHHHHHEECCCCCHHHEEEEEECCCCHHHHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377; 1936936