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The map label for this gene is pdhC

Identifier: 16078524

GI number: 16078524

Start: 1530537

End: 1531865

Strand: Direct

Name: pdhC

Synonym: BSU14600

Alternate gene names: 16078524

Gene position: 1530537-1531865 (Clockwise)

Preceding gene: 16078523

Following gene: 16078525

Centisome position: 36.31

GC content: 44.32

Gene sequence:

>1329_bases
GTGGCATTTGAATTTAAACTTCCAGATATCGGGGAAGGTATCCACGAAGGCGAAATCGTAAAATGGTTTGTCAAGCCTAA
CGACGAGGTAGACGAAGATGATGTACTGGCTGAAGTCCAAAATGATAAAGCAGTAGTAGAAATTCCTTCACCTGTTAAAG
GAAAAGTATTAGAATTAAAAGTTGAAGAGGGAACGGTTGCAACTGTTGGACAAACGATTATTACGTTTGATGCACCTGGT
TACGAAGATCTTCAATTTAAAGGCAGCGACGAGTCTGACGATGCGAAAACTGAAGCACAAGTTCAGTCAACTGCAGAAGC
TGGACAAGACGTTGCGAAAGAAGAGCAAGCTCAAGAGCCTGCAAAAGCTACTGGCGCAGGACAGCAGGATCAAGCTGAAG
TTGACCCGAACAAACGCGTGATCGCTATGCCTTCCGTACGTAAATATGCACGTGAAAAAGGTGTAGACATCCGTAAGGTT
ACTGGTTCAGGCAACAACGGACGTGTTGTAAAAGAAGATATCGACAGCTTTGTAAACGGAGGAGCGCAAGAAGCTGCACC
GCAAGAAACAGCTGCACCACAAGAAACAGCTGCTAAACCGGCTGCTGCACCAGCTCCAGAGGGCGAATTCCCAGAAACAC
GCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCAAAAGCGATGGTTAACTCTAAACACACTGCTCCTCACGTAACGTTA
ATGGACGAAGTGGACGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCT
GACTTACTTGCCTTACGTTGTAAAAGCTCTTACATCTGCACTGAAAAAATTCCCTGTTTTAAACACGTCAATTGACGATA
AAACAGATGAAGTCATCCAAAAACATTACTTCAACATCGGTATCGCTGCTGACACTGAAAAAGGCTTGCTTGTACCGGTT
GTGAAAAATGCAGATCGTAAATCTGTCTTTGAAATTTCTGATGAAATCAATGGCCTTGCAACAAAAGCTCGTGAAGGCAA
GCTTGCTCCAGCTGAAATGAAAGGCGCATCTTGCACAATTACAAACATCGGTTCTGCCGGCGGACAATGGTTCACTCCGG
TTATCAACCATCCAGAAGTTGCGATTCTTGGTATCGGACGCATTGCAGAAAAAGCGATTGTTCGTGATGGCGAAATCGTA
GCAGCTCCAGTCTTAGCTCTTTCTCTCAGCTTCGACCACCGTATGATCGACGGAGCAACTGCGCAAAATGCATTAAATCA
CATCAAGCGTTTACTGAACGATCCACAATTAATTTTAATGGAGGCGTAA

Upstream 100 bases:

>100_bases
CGAGAGGGAAGATGAACGTTTTCCCTCTATTATATATCTGTTTTCAATGCTTACGATGTAAACTTTAAAATTGCTTAATC
AAACTAGGAGGTCGAGAACT

Downstream 100 bases:

>100_bases
TGTTATGGTAGTAGGAGATTTCCCTATTGAAACAGATACTCTTGTAATTGGTGCGGGACCTGGCGGCTATGTAGCTGCCA
TCCGCGCTGCACAGCTTGGA

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2; S complex, 48 kDa subunit

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MAFEFKLPDIGEGIHEGEIVKWFVKPNDEVDEDDVLAEVQNDKAVVEIPSPVKGKVLELKVEEGTVATVGQTIITFDAPG
YEDLQFKGSDESDDAKTEAQVQSTAEAGQDVAKEEQAQEPAKATGAGQQDQAEVDPNKRVIAMPSVRKYAREKGVDIRKV
TGSGNNGRVVKEDIDSFVNGGAQEAAPQETAAPQETAAKPAAAPAPEGEFPETREKMSGIRKAIAKAMVNSKHTAPHVTL
MDEVDVTNLVAHRKQFKQVAADQGIKLTYLPYVVKALTSALKKFPVLNTSIDDKTDEVIQKHYFNIGIAADTEKGLLVPV
VKNADRKSVFEISDEINGLATKAREGKLAPAEMKGASCTITNIGSAGGQWFTPVINHPEVAILGIGRIAEKAIVRDGEIV
AAPVLALSLSFDHRMIDGATAQNALNHIKRLLNDPQLILMEA

Sequences:

>Translated_442_residues
MAFEFKLPDIGEGIHEGEIVKWFVKPNDEVDEDDVLAEVQNDKAVVEIPSPVKGKVLELKVEEGTVATVGQTIITFDAPG
YEDLQFKGSDESDDAKTEAQVQSTAEAGQDVAKEEQAQEPAKATGAGQQDQAEVDPNKRVIAMPSVRKYAREKGVDIRKV
TGSGNNGRVVKEDIDSFVNGGAQEAAPQETAAPQETAAKPAAAPAPEGEFPETREKMSGIRKAIAKAMVNSKHTAPHVTL
MDEVDVTNLVAHRKQFKQVAADQGIKLTYLPYVVKALTSALKKFPVLNTSIDDKTDEVIQKHYFNIGIAADTEKGLLVPV
VKNADRKSVFEISDEINGLATKAREGKLAPAEMKGASCTITNIGSAGGQWFTPVINHPEVAILGIGRIAEKAIVRDGEIV
AAPVLALSLSFDHRMIDGATAQNALNHIKRLLNDPQLILMEA
>Mature_441_residues
AFEFKLPDIGEGIHEGEIVKWFVKPNDEVDEDDVLAEVQNDKAVVEIPSPVKGKVLELKVEEGTVATVGQTIITFDAPGY
EDLQFKGSDESDDAKTEAQVQSTAEAGQDVAKEEQAQEPAKATGAGQQDQAEVDPNKRVIAMPSVRKYAREKGVDIRKVT
GSGNNGRVVKEDIDSFVNGGAQEAAPQETAAPQETAAKPAAAPAPEGEFPETREKMSGIRKAIAKAMVNSKHTAPHVTLM
DEVDVTNLVAHRKQFKQVAADQGIKLTYLPYVVKALTSALKKFPVLNTSIDDKTDEVIQKHYFNIGIAADTEKGLLVPVV
KNADRKSVFEISDEINGLATKAREGKLAPAEMKGASCTITNIGSAGGQWFTPVINHPEVAILGIGRIAEKAIVRDGEIVA
APVLALSLSFDHRMIDGATAQNALNHIKRLLNDPQLILMEA

Specific function: The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI110671329, Length=449, Percent_Identity=34.2984409799555, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI31711992, Length=436, Percent_Identity=30.9633027522936, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI19923748, Length=253, Percent_Identity=35.5731225296443, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI203098753, Length=461, Percent_Identity=27.3318872017354, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI203098816, Length=463, Percent_Identity=26.9978401727862, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI260898739, Length=158, Percent_Identity=34.1772151898734, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI1786305, Length=444, Percent_Identity=35.3603603603604, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1786946, Length=441, Percent_Identity=30.3854875283447, Blast_Score=211, Evalue=7e-56,
Organism=Caenorhabditis elegans, GI17537937, Length=449, Percent_Identity=31.8485523385301, Blast_Score=223, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI17560088, Length=455, Percent_Identity=29.2307692307692, Blast_Score=168, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI25146366, Length=230, Percent_Identity=34.7826086956522, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17538894, Length=308, Percent_Identity=29.5454545454545, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6320352, Length=455, Percent_Identity=29.8901098901099, Blast_Score=195, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6324258, Length=456, Percent_Identity=27.6315789473684, Blast_Score=150, Evalue=3e-37,
Organism=Drosophila melanogaster, GI18859875, Length=449, Percent_Identity=36.7483296213808, Blast_Score=249, Evalue=3e-66,
Organism=Drosophila melanogaster, GI24645909, Length=233, Percent_Identity=34.3347639484979, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24582497, Length=229, Percent_Identity=34.061135371179, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI20129315, Length=229, Percent_Identity=34.061135371179, Blast_Score=133, Evalue=3e-31,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ODP2_BACSU (P21883)

Other databases:

- EMBL:   M57435
- EMBL:   AF012285
- EMBL:   AL009126
- PIR:   D36718
- RefSeq:   NP_389343.1
- ProteinModelPortal:   P21883
- EnsemblBacteria:   EBBACT00000003193
- GeneID:   936010
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU14600
- NMPDR:   fig|224308.1.peg.1462
- GenoList:   BSU14600
- GeneTree:   EBGT00050000001223
- HOGENOM:   HBG630916
- OMA:   TSIDDKT
- PhylomeDB:   P21883
- ProtClustDB:   PRK11856
- BioCyc:   BSUB:BSU14600-MONOMER
- BRENDA:   2.3.1.12
- GO:   GO:0006096
- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- Gene3D:   G3DSA:4.10.320.10
- Gene3D:   G3DSA:3.30.559.10

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding; SSF47005 E3_bd; SSF51230 Hybrid_motif

EC number: =2.3.1.12

Molecular weight: Translated: 47539; Mature: 47408

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50968 BIOTINYL_LIPOYL; PS00189 LIPOYL

Important sites: ACT_SITE 413-413

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFEFKLPDIGEGIHEGEIVKWFVKPNDEVDEDDVLAEVQNDKAVVEIPSPVKGKVLELK
CCEEECCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCEEEECCCCCCCCEEEEE
VEEGTVATVGQTIITFDAPGYEDLQFKGSDESDDAKTEAQVQSTAEAGQDVAKEEQAQEP
ECCCCEEECCCEEEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
AKATGAGQQDQAEVDPNKRVIAMPSVRKYAREKGVDIRKVTGSGNNGRVVKEDIDSFVNG
HHHCCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCEEEEEECCCCCCEEEHHHHHHHHCC
GAQEAAPQETAAPQETAAKPAAAPAPEGEFPETREKMSGIRKAIAKAMVNSKHTAPHVTL
CCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
MDEVDVTNLVAHRKQFKQVAADQGIKLTYLPYVVKALTSALKKFPVLNTSIDDKTDEVIQ
ECCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
KHYFNIGIAADTEKGLLVPVVKNADRKSVFEISDEINGLATKAREGKLAPAEMKGASCTI
HHHHEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCEEEE
TNIGSAGGQWFTPVINHPEVAILGIGRIAEKAIVRDGEIVAAPVLALSLSFDHRMIDGAT
EECCCCCCCEECCCCCCCCEEEEECHHHHHHHHHCCCCEEEECHHHEEECCCCHHCCCHH
AQNALNHIKRLLNDPQLILMEA
HHHHHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure 
AFEFKLPDIGEGIHEGEIVKWFVKPNDEVDEDDVLAEVQNDKAVVEIPSPVKGKVLELK
CEEECCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCEEEECCCCCCCCEEEEE
VEEGTVATVGQTIITFDAPGYEDLQFKGSDESDDAKTEAQVQSTAEAGQDVAKEEQAQEP
ECCCCEEECCCEEEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
AKATGAGQQDQAEVDPNKRVIAMPSVRKYAREKGVDIRKVTGSGNNGRVVKEDIDSFVNG
HHHCCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCEEEEEECCCCCCEEEHHHHHHHHCC
GAQEAAPQETAAPQETAAKPAAAPAPEGEFPETREKMSGIRKAIAKAMVNSKHTAPHVTL
CCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
MDEVDVTNLVAHRKQFKQVAADQGIKLTYLPYVVKALTSALKKFPVLNTSIDDKTDEVIQ
ECCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
KHYFNIGIAADTEKGLLVPVVKNADRKSVFEISDEINGLATKAREGKLAPAEMKGASCTI
HHHHEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCEEEE
TNIGSAGGQWFTPVINHPEVAILGIGRIAEKAIVRDGEIVAAPVLALSLSFDHRMIDGAT
EECCCCCCCEECCCCCCCCEEEEECHHHHHHHHHCCCCEEEECHHHEEECCCCHHCCCHH
AQNALNHIKRLLNDPQLILMEA
HHHHHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377