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The map label for this gene is pdhA

Identifier: 16078522

GI number: 16078522

Start: 1528326

End: 1529441

Strand: Direct

Name: pdhA

Synonym: BSU14580

Alternate gene names: 16078522

Gene position: 1528326-1529441 (Clockwise)

Preceding gene: 255767337

Following gene: 16078523

Centisome position: 36.25

GC content: 43.37

Gene sequence:

>1116_bases
ATGGCTGCAAAAACGAAAAAAGCTATCGTTGACAGTAAGAAGCAATTTGATGCCATTAAAAAGCAGTTCGAAACGTTCCA
AATTTTAAATGAAAAGGGAGAAGTCGTGAATGAAGCGGCGATGCCTGATTTAACTGATGATCAATTAAAAGAGCTAATGC
GCCGCATGGTATTTACGCGTGTGCTTGACCAACGCTCTATCTCATTAAACCGTCAAGGACGTCTCGGATTTTACGCTCCT
ACTGCGGGTCAAGAAGCTTCTCAGATTGCAACGCATTTCGCGCTTGAAAAAGAAGACTTTGTTCTTCCTGGATACCGTGA
TGTGCCTCAGTTAATTTGGCACGGCCTTCCATTATATCAAGCGTTTCTTTTCTCTCGCGGACATTTCAGAGGAAACCAAA
TGCCTGATGATGTGAATGCCCTTTCTCCACAAATCATTATCGGTGCTCAATACATTCAAACTGCCGGTGTTGCGCTAGGT
CTTAAAAAACGCGGTAAGAAAGCTGTCGCAATCACTTACACTGGTGACGGCGGAGCTTCTCAAGGGGACTTCTACGAAGG
AATTAACTTTGCCGGAGCTTATAAAGCACCTGCAATCTTCGTGGTGCAAAACAACCGTTACGCGATTTCAACTCCTGTTG
AAAAACAATCTGCAGCTGAAACAATTGCACAAAAAGCTGTAGCTGCCGGTATTGTCGGCGTACAAGTAGACGGAATGGAT
CCGCTTGCTGTATACGCAGCAACTGCTGAAGCACGCGAGCGCGCAATCAACGGCGAAGGTCCAACACTAATTGAAACACT
TACATTCCGTTATGGCCCGCACACAATGGCTGGTGACGATCCTACTAAATATCGTACAAAAGAAATCGAAAATGAGTGGG
AACAAAAAGATCCGCTTGTACGTTTCCGTGCGTTCCTTGAAAACAAAGGCTTATGGTCTGAAGAAGAAGAAGCAAAAGTG
ATTGAGGATGCGAAAGAAGAAATTAAGCAAGCGATCAAAAAAGCTGATGCTGAACCGAAGCAAAAAGTAACTGATTTAAT
GAAAATCATGTACGAAAAAATGCCTCACAACCTTGAGGAGCAATTTGAAATTTATACACAGAAGGAGTCGAAGTAA

Upstream 100 bases:

>100_bases
ATACGACTTACTGCTGATACTTTAGGGTACCCTAAGTTTGTATGTATAAAAGGAATGCACGTCTAATGACTGTTTAAAAG
AAAGGAAGAGGTGACTTAGT

Downstream 100 bases:

>100_bases
GCCATGGCGCAAATGACAATGATTCAAGCAATCACGGATGCGTTACGCACAGAACTGAAAAATGACGAAAATGTCTTAGT
TTTCGGAGAAGACGTTGGTG

Product: pyruvate dehydrogenase (E1 alpha subunit)

Products: NA

Alternate protein names: S complex, 42 kDa subunit; Vegetative protein 220; VEG220

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MAAKTKKAIVDSKKQFDAIKKQFETFQILNEKGEVVNEAAMPDLTDDQLKELMRRMVFTRVLDQRSISLNRQGRLGFYAP
TAGQEASQIATHFALEKEDFVLPGYRDVPQLIWHGLPLYQAFLFSRGHFRGNQMPDDVNALSPQIIIGAQYIQTAGVALG
LKKRGKKAVAITYTGDGGASQGDFYEGINFAGAYKAPAIFVVQNNRYAISTPVEKQSAAETIAQKAVAAGIVGVQVDGMD
PLAVYAATAEARERAINGEGPTLIETLTFRYGPHTMAGDDPTKYRTKEIENEWEQKDPLVRFRAFLENKGLWSEEEEAKV
IEDAKEEIKQAIKKADAEPKQKVTDLMKIMYEKMPHNLEEQFEIYTQKESK

Sequences:

>Translated_371_residues
MAAKTKKAIVDSKKQFDAIKKQFETFQILNEKGEVVNEAAMPDLTDDQLKELMRRMVFTRVLDQRSISLNRQGRLGFYAP
TAGQEASQIATHFALEKEDFVLPGYRDVPQLIWHGLPLYQAFLFSRGHFRGNQMPDDVNALSPQIIIGAQYIQTAGVALG
LKKRGKKAVAITYTGDGGASQGDFYEGINFAGAYKAPAIFVVQNNRYAISTPVEKQSAAETIAQKAVAAGIVGVQVDGMD
PLAVYAATAEARERAINGEGPTLIETLTFRYGPHTMAGDDPTKYRTKEIENEWEQKDPLVRFRAFLENKGLWSEEEEAKV
IEDAKEEIKQAIKKADAEPKQKVTDLMKIMYEKMPHNLEEQFEIYTQKESK
>Mature_370_residues
AAKTKKAIVDSKKQFDAIKKQFETFQILNEKGEVVNEAAMPDLTDDQLKELMRRMVFTRVLDQRSISLNRQGRLGFYAPT
AGQEASQIATHFALEKEDFVLPGYRDVPQLIWHGLPLYQAFLFSRGHFRGNQMPDDVNALSPQIIIGAQYIQTAGVALGL
KKRGKKAVAITYTGDGGASQGDFYEGINFAGAYKAPAIFVVQNNRYAISTPVEKQSAAETIAQKAVAAGIVGVQVDGMDP
LAVYAATAEARERAINGEGPTLIETLTFRYGPHTMAGDDPTKYRTKEIENEWEQKDPLVRFRAFLENKGLWSEEEEAKVI
EDAKEEIKQAIKKADAEPKQKVTDLMKIMYEKMPHNLEEQFEIYTQKESK

Specific function: The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11386135, Length=348, Percent_Identity=32.183908045977, Blast_Score=199, Evalue=5e-51,
Organism=Homo sapiens, GI258645172, Length=348, Percent_Identity=32.183908045977, Blast_Score=195, Evalue=7e-50,
Organism=Homo sapiens, GI291084742, Length=331, Percent_Identity=29.9093655589124, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI4505685, Length=333, Percent_Identity=29.4294294294294, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI291084744, Length=211, Percent_Identity=36.9668246445498, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI4885543, Length=320, Percent_Identity=29.375, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI291084757, Length=316, Percent_Identity=29.1139240506329, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI86563355, Length=347, Percent_Identity=31.9884726224784, Blast_Score=194, Evalue=9e-50,
Organism=Caenorhabditis elegans, GI86563357, Length=347, Percent_Identity=31.9884726224784, Blast_Score=194, Evalue=9e-50,
Organism=Caenorhabditis elegans, GI32564172, Length=319, Percent_Identity=31.0344827586207, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17536047, Length=319, Percent_Identity=31.0344827586207, Blast_Score=135, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6321026, Length=343, Percent_Identity=28.2798833819242, Blast_Score=129, Evalue=7e-31,
Organism=Drosophila melanogaster, GI21355903, Length=348, Percent_Identity=30.4597701149425, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24639744, Length=330, Percent_Identity=30, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI28571106, Length=330, Percent_Identity=30, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24639740, Length=330, Percent_Identity=30, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24639746, Length=297, Percent_Identity=30.6397306397306, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24639748, Length=288, Percent_Identity=32.2916666666667, Blast_Score=122, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ODPA_BACSU (P21881)

Other databases:

- EMBL:   M57435
- EMBL:   AF012285
- EMBL:   AL009126
- PIR:   B36718
- RefSeq:   NP_389341.1
- ProteinModelPortal:   P21881
- SMR:   P21881
- EnsemblBacteria:   EBBACT00000000307
- GeneID:   936005
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU14580
- NMPDR:   fig|224308.1.peg.1460
- GenoList:   BSU14580
- GeneTree:   EBGT00050000001071
- HOGENOM:   HBG753263
- OMA:   RYRTKED
- ProtClustDB:   CLSK2460915
- BioCyc:   BSUB:BSU14580-MONOMER
- BRENDA:   1.2.4.1
- GO:   GO:0006096
- InterPro:   IPR001017
- InterPro:   IPR017596
- TIGRFAMs:   TIGR03181

Pfam domain/function: PF00676 E1_dh

EC number: =1.2.4.1

Molecular weight: Translated: 41549; Mature: 41418

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00037 MYB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKTKKAIVDSKKQFDAIKKQFETFQILNEKGEVVNEAAMPDLTDDQLKELMRRMVFTR
CCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
VLDQRSISLNRQGRLGFYAPTAGQEASQIATHFALEKEDFVLPGYRDVPQLIWHGLPLYQ
HHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCEECCCCCHHHHHHHCCCHHHH
AFLFSRGHFRGNQMPDDVNALSPQIIIGAQYIQTAGVALGLKKRGKKAVAITYTGDGGAS
HHHHHCCCCCCCCCCCCHHHCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCC
QGDFYEGINFAGAYKAPAIFVVQNNRYAISTPVEKQSAAETIAQKAVAAGIVGVQVDGMD
CCCHHHCCCCCCCCCCCEEEEEECCCEEEECCCHHHHHHHHHHHHHHHHCEEEEEECCCC
PLAVYAATAEARERAINGEGPTLIETLTFRYGPHTMAGDDPTKYRTKEIENEWEQKDPLV
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHH
RFRAFLENKGLWSEEEEAKVIEDAKEEIKQAIKKADAEPKQKVTDLMKIMYEKMPHNLEE
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHH
QFEIYTQKESK
HHHHHHCCCCC
>Mature Secondary Structure 
AAKTKKAIVDSKKQFDAIKKQFETFQILNEKGEVVNEAAMPDLTDDQLKELMRRMVFTR
CCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
VLDQRSISLNRQGRLGFYAPTAGQEASQIATHFALEKEDFVLPGYRDVPQLIWHGLPLYQ
HHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCEECCCCCHHHHHHHCCCHHHH
AFLFSRGHFRGNQMPDDVNALSPQIIIGAQYIQTAGVALGLKKRGKKAVAITYTGDGGAS
HHHHHCCCCCCCCCCCCHHHCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCC
QGDFYEGINFAGAYKAPAIFVVQNNRYAISTPVEKQSAAETIAQKAVAAGIVGVQVDGMD
CCCHHHCCCCCCCCCCCEEEEEECCCEEEECCCHHHHHHHHHHHHHHHHCEEEEEECCCC
PLAVYAATAEARERAINGEGPTLIETLTFRYGPHTMAGDDPTKYRTKEIENEWEQKDPLV
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHH
RFRAFLENKGLWSEEEEAKVIEDAKEEIKQAIKKADAEPKQKVTDLMKIMYEKMPHNLEE
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHH
QFEIYTQKESK
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377; 9298659