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The map label for this gene is pdhB

Identifier: 16078523

GI number: 16078523

Start: 1529445

End: 1530422

Strand: Direct

Name: pdhB

Synonym: BSU14590

Alternate gene names: 16078523

Gene position: 1529445-1530422 (Clockwise)

Preceding gene: 16078522

Following gene: 16078524

Centisome position: 36.28

GC content: 44.99

Gene sequence:

>978_bases
ATGGCGCAAATGACAATGATTCAAGCAATCACGGATGCGTTACGCACAGAACTGAAAAATGACGAAAATGTCTTAGTTTT
CGGAGAAGACGTTGGTGTAAACGGCGGCGTATTCCGTGCGACAGAAGGATTGCAAAAAGAATTCGGTGAAGACCGTGTGT
TTGACACGCCACTTGCTGAATCTGGTATCGGCGGTCTTGCCCTTGGTTTAGGCTTAAACGGCTTCCGTCCGGTAATGGAA
ATCCAATTCTTCGGATTTGTTTATGAAGTAATGGATTCAGTTTCTGGCCAAATGGCTCGTATGCGCTACCGTTCTGGCGG
ACGCTGGACTTCACCTGTAACAATTCGTTCTCCATTCGGCGGCGGTGTTCATACTCCTGAACTTCACGCTGACAGCTTGG
AAGGTCTTGTTGCACAACAGCCTGGTATCAAAGTTGTTATTCCATCAACTCCTTACGATGCCAAAGGACTTTTAATTTCT
GCGATCAGAGACAATGATCCTGTTGTCTTCTTAGAGCATATGAAGCTTTACCGTTCTTTCCGTCAGGAAGTTCCTGAAGA
AGAATACACAATTGAGCTTGGAAAAGCTGACGTGAAACGTGAAGGTACTGATCTTTCAATCATCACTTACGGCGCAATGG
TTCATGAATCATTAAAAGCTGCTGATGAGCTTGAAAAAGACGGCATTTCTGCTGAAGTTGTCGACCTTCGTACAGTAAGC
CCACTTGATATCGATACAATTATCGCCTCTGTAGAAAAAACAGGACGCGCGATTGTCGTTCAAGAGGCACAAAAACAAGC
CGGTATTGCTGCAAACGTAGTAGCAGAAATTAATGACCGTGCGATCCTGAGCTTGGAAGCACCTGTACTTCGCGTTGCAG
CGCCAGATACAGTATTTCCTTTCTCTCAAGCGGAGAGCGTATGGCTTCCAAACCATAAAGACGTTCTTGAAACAGCAAGA
AAAGTGCTTGAATTTTAA

Upstream 100 bases:

>100_bases
GCAAAAAGTAACTGATTTAATGAAAATCATGTACGAAAAAATGCCTCACAACCTTGAGGAGCAATTTGAAATTTATACAC
AGAAGGAGTCGAAGTAAGCC

Downstream 100 bases:

>100_bases
TCAAACTGCATAATCGAGAGGGAAGATGAACGTTTTCCCTCTATTATATATCTGTTTTCAATGCTTACGATGTAAACTTT
AAAATTGCTTAATCAAACTA

Product: pyruvate dehydrogenase (E1 beta subunit)

Products: NA

Alternate protein names: S complex, 36 kDa subunit

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MAQMTMIQAITDALRTELKNDENVLVFGEDVGVNGGVFRATEGLQKEFGEDRVFDTPLAESGIGGLALGLGLNGFRPVME
IQFFGFVYEVMDSVSGQMARMRYRSGGRWTSPVTIRSPFGGGVHTPELHADSLEGLVAQQPGIKVVIPSTPYDAKGLLIS
AIRDNDPVVFLEHMKLYRSFRQEVPEEEYTIELGKADVKREGTDLSIITYGAMVHESLKAADELEKDGISAEVVDLRTVS
PLDIDTIIASVEKTGRAIVVQEAQKQAGIAANVVAEINDRAILSLEAPVLRVAAPDTVFPFSQAESVWLPNHKDVLETAR
KVLEF

Sequences:

>Translated_325_residues
MAQMTMIQAITDALRTELKNDENVLVFGEDVGVNGGVFRATEGLQKEFGEDRVFDTPLAESGIGGLALGLGLNGFRPVME
IQFFGFVYEVMDSVSGQMARMRYRSGGRWTSPVTIRSPFGGGVHTPELHADSLEGLVAQQPGIKVVIPSTPYDAKGLLIS
AIRDNDPVVFLEHMKLYRSFRQEVPEEEYTIELGKADVKREGTDLSIITYGAMVHESLKAADELEKDGISAEVVDLRTVS
PLDIDTIIASVEKTGRAIVVQEAQKQAGIAANVVAEINDRAILSLEAPVLRVAAPDTVFPFSQAESVWLPNHKDVLETAR
KVLEF
>Mature_324_residues
AQMTMIQAITDALRTELKNDENVLVFGEDVGVNGGVFRATEGLQKEFGEDRVFDTPLAESGIGGLALGLGLNGFRPVMEI
QFFGFVYEVMDSVSGQMARMRYRSGGRWTSPVTIRSPFGGGVHTPELHADSLEGLVAQQPGIKVVIPSTPYDAKGLLISA
IRDNDPVVFLEHMKLYRSFRQEVPEEEYTIELGKADVKREGTDLSIITYGAMVHESLKAADELEKDGISAEVVDLRTVSP
LDIDTIIASVEKTGRAIVVQEAQKQAGIAANVVAEINDRAILSLEAPVLRVAAPDTVFPFSQAESVWLPNHKDVLETARK
VLEF

Specific function: The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4557353, Length=325, Percent_Identity=41.5384615384615, Blast_Score=266, Evalue=1e-71,
Organism=Homo sapiens, GI34101272, Length=325, Percent_Identity=41.5384615384615, Blast_Score=266, Evalue=1e-71,
Organism=Homo sapiens, GI156564403, Length=326, Percent_Identity=35.2760736196319, Blast_Score=219, Evalue=4e-57,
Organism=Homo sapiens, GI291084858, Length=326, Percent_Identity=33.7423312883436, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI225637463, Length=254, Percent_Identity=29.9212598425197, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI225637459, Length=254, Percent_Identity=29.9212598425197, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI225637461, Length=254, Percent_Identity=29.9212598425197, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI133778974, Length=97, Percent_Identity=40.2061855670103, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17506935, Length=326, Percent_Identity=39.8773006134969, Blast_Score=239, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI17538422, Length=328, Percent_Identity=36.5853658536585, Blast_Score=222, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6319698, Length=327, Percent_Identity=38.2262996941896, Blast_Score=217, Evalue=3e-57,
Organism=Drosophila melanogaster, GI160714832, Length=326, Percent_Identity=39.8773006134969, Blast_Score=245, Evalue=4e-65,
Organism=Drosophila melanogaster, GI160714828, Length=326, Percent_Identity=39.8773006134969, Blast_Score=244, Evalue=4e-65,
Organism=Drosophila melanogaster, GI21358145, Length=326, Percent_Identity=38.0368098159509, Blast_Score=228, Evalue=4e-60,
Organism=Drosophila melanogaster, GI24650940, Length=326, Percent_Identity=38.0368098159509, Blast_Score=228, Evalue=4e-60,
Organism=Drosophila melanogaster, GI24650943, Length=90, Percent_Identity=38.8888888888889, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24650945, Length=90, Percent_Identity=38.8888888888889, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ODPB_BACSU (P21882)

Other databases:

- EMBL:   M57435
- EMBL:   AF012285
- EMBL:   AL009126
- PIR:   C36718
- RefSeq:   NP_389342.1
- ProteinModelPortal:   P21882
- SMR:   P21882
- PhosSite:   P21882
- EnsemblBacteria:   EBBACT00000003000
- GeneID:   939496
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU14590
- NMPDR:   fig|224308.1.peg.1461
- GenoList:   BSU14590
- GeneTree:   EBGT00050000000572
- HOGENOM:   HBG753264
- OMA:   TYGAMVH
- ProtClustDB:   CLSK887245
- BioCyc:   BSUB:BSU14590-MONOMER
- BRENDA:   1.2.4.1
- GO:   GO:0006096
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476
- Gene3D:   G3DSA:3.40.50.920
- SMART:   SM00861

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C; SSF52922 Transketo_C_like

EC number: =1.2.4.1

Molecular weight: Translated: 35475; Mature: 35344

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: BINDING 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQMTMIQAITDALRTELKNDENVLVFGEDVGVNGGVFRATEGLQKEFGEDRVFDTPLAE
CCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEHHHHHHHHHCCCCCCCCCCCHH
SGIGGLALGLGLNGFRPVMEIQFFGFVYEVMDSVSGQMARMRYRSGGRWTSPVTIRSPFG
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCC
GGVHTPELHADSLEGLVAQQPGIKVVIPSTPYDAKGLLISAIRDNDPVVFLEHMKLYRSF
CCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEEECCCCEEEHHHHHHHHHH
RQEVPEEEYTIELGKADVKREGTDLSIITYGAMVHESLKAADELEKDGISAEVVDLRTVS
HHHCCCCCEEEEECCCHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
PLDIDTIIASVEKTGRAIVVQEAQKQAGIAANVVAEINDRAILSLEAPVLRVAAPDTVFP
CCCHHHHHHHHHHCCCEEEEECHHHHCCCCEEHHEECCCCEEEEECCCEEEEECCCCCCC
FSQAESVWLPNHKDVLETARKVLEF
CCCCCCEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
AQMTMIQAITDALRTELKNDENVLVFGEDVGVNGGVFRATEGLQKEFGEDRVFDTPLAE
CHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEHHHHHHHHHCCCCCCCCCCCHH
SGIGGLALGLGLNGFRPVMEIQFFGFVYEVMDSVSGQMARMRYRSGGRWTSPVTIRSPFG
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCC
GGVHTPELHADSLEGLVAQQPGIKVVIPSTPYDAKGLLISAIRDNDPVVFLEHMKLYRSF
CCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEEECCCCEEEHHHHHHHHHH
RQEVPEEEYTIELGKADVKREGTDLSIITYGAMVHESLKAADELEKDGISAEVVDLRTVS
HHHCCCCCEEEEECCCHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
PLDIDTIIASVEKTGRAIVVQEAQKQAGIAANVVAEINDRAILSLEAPVLRVAAPDTVFP
CCCHHHHHHHHHHCCCEEEEECHHHHCCCCEEHHEECCCCEEEEECCCEEEEECCCCCCC
FSQAESVWLPNHKDVLETARKVLEF
CCCCCCEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377