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The map label for this gene is ykuU

Identifier: 16078486

GI number: 16078486

Start: 1492261

End: 1492803

Strand: Direct

Name: ykuU

Synonym: BSU14220

Alternate gene names: 16078486

Gene position: 1492261-1492803 (Clockwise)

Preceding gene: 16078484

Following gene: 255767334

Centisome position: 35.4

GC content: 44.01

Gene sequence:

>543_bases
ATGGCAGAACGTATGGTAGGTAAACAAGCTCCTCGTTTTGAAATGGAAGCAGTCCTTGCAAGCAAAGAATTCGGCAAAGT
AAGTCTTGAGGAAAACATGAAAAACGACAAATGGACAGTGCTGTTCTTCTATCCAATGGACTTCACTTTTGTTTGTCCGA
CAGAAATTACAGCAATGTCTGACCGCTATGACGAGTTCGAAGATCTTGATGCAGAAGTCATCGGGGTTTCAACTGATACC
ATCCATACTCATTTAGCTTGGATCAATACAGACCGCAAAGAAAACGGTCTTGGCCAGCTGAAATACCCGCTTGCTGCTGA
TACAAACCATGAGGTATCCCGGGAATATGGCGTTTTAATCGAAGAAGAAGGTGTGGCTTTGCGCGGATTGTTTATTATTA
ATCCTGAAGGGGAGCTTCAATATCAAACTGTTTTCCATAACAACATTGGGCGTGATGTTGATGAAACGCTTCGTGTTCTC
CAGGCGCTGCAAACTGGCGGACTCTGTCCGGCTAACTGGAAACCAGGCCAAAAAACACTTTAA

Upstream 100 bases:

>100_bases
TTTATATAATGGCATTGCAATATAACTAAAAATGGTTAATCACCCTTTATTTTCATGCGATTTTGACCTTTAAGCCAAAC
AATAAATTGGAGGGATACAT

Downstream 100 bases:

>100_bases
TTCTTTCCAAGAACGAAAAGCGGTCTAGCATATTCGTTAGGCCTCTCTTTTAAAATAGGAGGGAAAAAGCAATGAAATTA
CGTCAGCCCATGCCGGAATT

Product: 2-cys peroxiredoxin

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 180; Mature: 179

Protein sequence:

>180_residues
MAERMVGKQAPRFEMEAVLASKEFGKVSLEENMKNDKWTVLFFYPMDFTFVCPTEITAMSDRYDEFEDLDAEVIGVSTDT
IHTHLAWINTDRKENGLGQLKYPLAADTNHEVSREYGVLIEEEGVALRGLFIINPEGELQYQTVFHNNIGRDVDETLRVL
QALQTGGLCPANWKPGQKTL

Sequences:

>Translated_180_residues
MAERMVGKQAPRFEMEAVLASKEFGKVSLEENMKNDKWTVLFFYPMDFTFVCPTEITAMSDRYDEFEDLDAEVIGVSTDT
IHTHLAWINTDRKENGLGQLKYPLAADTNHEVSREYGVLIEEEGVALRGLFIINPEGELQYQTVFHNNIGRDVDETLRVL
QALQTGGLCPANWKPGQKTL
>Mature_179_residues
AERMVGKQAPRFEMEAVLASKEFGKVSLEENMKNDKWTVLFFYPMDFTFVCPTEITAMSDRYDEFEDLDAEVIGVSTDTI
HTHLAWINTDRKENGLGQLKYPLAADTNHEVSREYGVLIEEEGVALRGLFIINPEGELQYQTVFHNNIGRDVDETLRVLQ
ALQTGGLCPANWKPGQKTL

Specific function: Directly Reduces Organic Hydroperoxides In Its Reduced Dithiol Form. [C]

COG id: COG0450

COG function: function code O; Peroxiredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain

Homologues:

Organism=Homo sapiens, GI4505591, Length=179, Percent_Identity=48.0446927374302, Blast_Score=181, Evalue=2e-46,
Organism=Homo sapiens, GI32455266, Length=179, Percent_Identity=48.0446927374302, Blast_Score=181, Evalue=2e-46,
Organism=Homo sapiens, GI32455264, Length=179, Percent_Identity=48.0446927374302, Blast_Score=181, Evalue=2e-46,
Organism=Homo sapiens, GI32189392, Length=179, Percent_Identity=52.5139664804469, Blast_Score=180, Evalue=5e-46,
Organism=Homo sapiens, GI5802974, Length=179, Percent_Identity=49.1620111731844, Blast_Score=179, Evalue=1e-45,
Organism=Homo sapiens, GI32483377, Length=177, Percent_Identity=49.7175141242938, Blast_Score=179, Evalue=1e-45,
Organism=Homo sapiens, GI5453549, Length=184, Percent_Identity=46.7391304347826, Blast_Score=175, Evalue=2e-44,
Organism=Homo sapiens, GI4758638, Length=189, Percent_Identity=30.6878306878307, Blast_Score=91, Evalue=5e-19,
Organism=Homo sapiens, GI33188454, Length=95, Percent_Identity=47.3684210526316, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1786822, Length=159, Percent_Identity=38.3647798742138, Blast_Score=125, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI32565831, Length=184, Percent_Identity=51.6304347826087, Blast_Score=197, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI193204376, Length=184, Percent_Identity=51.6304347826087, Blast_Score=197, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17554494, Length=180, Percent_Identity=47.7777777777778, Blast_Score=165, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI25153706, Length=189, Percent_Identity=26.984126984127, Blast_Score=80, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6323613, Length=179, Percent_Identity=50.8379888268156, Blast_Score=173, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6320661, Length=179, Percent_Identity=48.6033519553073, Blast_Score=169, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6319407, Length=188, Percent_Identity=27.6595744680851, Blast_Score=96, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24656348, Length=180, Percent_Identity=52.7777777777778, Blast_Score=188, Evalue=2e-48,
Organism=Drosophila melanogaster, GI17864676, Length=180, Percent_Identity=52.7777777777778, Blast_Score=188, Evalue=2e-48,
Organism=Drosophila melanogaster, GI17157991, Length=177, Percent_Identity=52.5423728813559, Blast_Score=179, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24641739, Length=177, Percent_Identity=52.5423728813559, Blast_Score=179, Evalue=1e-45,
Organism=Drosophila melanogaster, GI17738015, Length=179, Percent_Identity=48.6033519553073, Blast_Score=176, Evalue=6e-45,
Organism=Drosophila melanogaster, GI21357347, Length=181, Percent_Identity=45.3038674033149, Blast_Score=166, Evalue=8e-42,
Organism=Drosophila melanogaster, GI17975518, Length=189, Percent_Identity=31.2169312169312, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24652434, Length=189, Percent_Identity=31.2169312169312, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24652436, Length=189, Percent_Identity=31.2169312169312, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24581278, Length=185, Percent_Identity=30.8108108108108, Blast_Score=100, Evalue=9e-22,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2250 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 6040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1580 Molecules/Cell In: Stationary-Phase

Swissprot (AC and ID): YKUU_BACSU (O34564)

Other databases:

- EMBL:   AJ222587
- EMBL:   AL009126
- PIR:   B69867
- RefSeq:   NP_389305.1
- HSSP:   P32119
- ProteinModelPortal:   O34564
- SMR:   O34564
- EnsemblBacteria:   EBBACT00000003962
- GeneID:   938810
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU14220
- NMPDR:   fig|224308.1.peg.1424
- GenoList:   BSU14220
- GeneTree:   EBGT00050000000759
- HOGENOM:   HBG493509
- OMA:   KEYFAAI
- PhylomeDB:   O34564
- ProtClustDB:   CLSK873385
- BioCyc:   BSUB:BSU14220-MONOMER
- InterPro:   IPR000866
- InterPro:   IPR019479
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR012335
- Gene3D:   G3DSA:3.40.30.10

Pfam domain/function: PF10417 1-cysPrx_C; PF00578 AhpC-TSA; SSF52833 Thiordxn-like_fd

EC number: 1.6.4.- [C]

Molecular weight: Translated: 20447; Mature: 20316

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00194 THIOREDOXIN_1; PS51352 THIOREDOXIN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERMVGKQAPRFEMEAVLASKEFGKVSLEENMKNDKWTVLFFYPMDFTFVCPTEITAMS
CCCCCCCCCCCCHHHHHHHHHCCCCCEEHHHCCCCCCEEEEEEECCCEEEECCCCHHHHH
DRYDEFEDLDAEVIGVSTDTIHTHLAWINTDRKENGLGQLKYPLAADTNHEVSREYGVLI
HCCCHHHCCCCEEEEECCCCEEEEEEEEECCCCCCCCCEEECCEECCCCCCCHHHHCEEE
EEEGVALRGLFIINPEGELQYQTVFHNNIGRDVDETLRVLQALQTGGLCPANWKPGQKTL
CCCCEEEEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AERMVGKQAPRFEMEAVLASKEFGKVSLEENMKNDKWTVLFFYPMDFTFVCPTEITAMS
CCCCCCCCCCCHHHHHHHHHCCCCCEEHHHCCCCCCEEEEEEECCCEEEECCCCHHHHH
DRYDEFEDLDAEVIGVSTDTIHTHLAWINTDRKENGLGQLKYPLAADTNHEVSREYGVLI
HCCCHHHCCCCEEEEECCCCEEEEEEEEECCCCCCCCCEEECCEECCCCCCCHHHHCEEE
EEEGVALRGLFIINPEGELQYQTVFHNNIGRDVDETLRVLQALQTGGLCPANWKPGQKTL
CCCCEEEEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377