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The map label for this gene is kinA

Identifier: 16078463

GI number: 16078463

Start: 1470026

End: 1471846

Strand: Direct

Name: kinA

Synonym: BSU13990

Alternate gene names: 16078463

Gene position: 1470026-1471846 (Clockwise)

Preceding gene: 16078462

Following gene: 16078465

Centisome position: 34.87

GC content: 40.53

Gene sequence:

>1821_bases
GTGGAACAGGATACGCAGCATGTTAAACCACTTCAAACAAAAACCGATATTCATGCAGTCTTGGCCTCTAATGGACGCAT
CATTTATATATCTGCCAACTCCAAACTGCATTTGGGCTATCTCCAAGGAGAGATGATCGGATCATTCCTCAAAACGTTTC
TGCATGAGGAAGACCAATTTTTGGTTGAAAGCTATTTTTATAATGAACATCATCTGATGCCGTGCACCTTTCGTTTTATT
AAAAAAGATCATACGATTGTGTGGGTGGAGGCTGCGGTAGAAATTGTTACGACAAGAGCTGAGCGGACAGAACGGGAAAT
CATTTTGAAAATGAAGGTTCTTGAAGAAGAAACAGGCCATCAATCCCTAAACTGCGAAAAACATGAAATCGAACCTGCAA
GCCCGGAATCGACTACATATATAACGGATGATTATGAACGGTTGGTTGAAAATCTCCCGAGTCCGCTATGCATCAGTGTC
AAAGGCAAGATCGTCTATGTAAACAGCGCGATGCTTTCAATGCTGGGAGCCAAAAGCAAGGATGCTATTATTGGTAAATC
GTCCTATGAATTTATTGAAGAAGAATATCATGATATCGTGAAAAACAGGATTATACGAATGCAAAAAGGAATGGAAGTCG
GAATGATTGAACAGACGTGGAAAAGGCTTGATGGCACACCTGTTCATTTAGAAGTGAAAGCATCCCCGACCGTCTACAAA
AACCAGCAGGCTGAGCTGCTGCTGCTGATCGATATCTCTTCAAGGAAAAAATTCCAAACCATCCTGCAAAAAAGCCGTGA
ACGATATCAGCTGCTGATTCAAAATTCCATTGATACCATTGCGGTGATTCACAATGGAAAATGGGTATTTATGAATGAAT
CGGGAATTTCCCTGTTTGAAGCGGCTACATATGAGGATTTAATTGGCAAAAACATATACGATCAGCTGCATCCTTGCGAT
CACGAGGATGTAAAAGAGAGAATCCAAAACATTGCCGAGCAAAAAACAGAATCTGAAATTGTCAAGCAATCCTGGTTCAC
CTTTCAGAACAGGGTCATCTATACGGAGATGGTCTGCATTCCGACGACCTTTTTTGGTGAAGCGGCCGTCCAGGTCATTC
TTCGGGACATCTCAGAGAGAAAACAAACAGAAGAATTGATGCTGAAATCGGAAAAATTATCAATCGCAGGGCAGCTCGCG
GCGGGAATCGCCCATGAGATCCGCAACCCTCTTACAGCGATCAAAGGATTTTTACAGCTGATGAAACCGACAATGGAAGG
CAACGAACATTACTTTGATATTGTGTTTTCTGAACTCAGCCGTATCGAATTAATACTCAGTGAACTGCTCATGCTGGCGA
AACCTCAGCAAAATGCTGTCAAAGAATATTTGAACTTGAAAAAATTAATTGGTGAGGTTTCAGCCCTGTTAGAAACGCAG
GCGAATTTAAATGGCATTTTTATCAGAACAAGTTATGAAAAAGACAGCATTTATATAAACGGGGATCAAAACCAATTAAA
GCAGGTATTCATTAATTTAATCAAAAATGCAGTTGAATCAATGCCTGATGGGGGAACAGTAGACATTATCATAACCGAAG
ATGAGCATTCTGTTCATGTTACTGTCAAAGACGAAGGGGAAGGTATACCTGAAAAGGTACTAAACCGGATTGGAGAGCCA
TTTTTAACAACAAAAGAAAAAGGTACGGGGCTTGGATTAATGGTGACATTTAATATCATTGAAAACCATCAGGGAGTTAT
ACATGTGGACAGCCATCCTGAAAAAGGCACAGCGTTTAAAATTTCATTTCCAAAAAAATAA

Upstream 100 bases:

>100_bases
ATAAGAATAGAAGGAGAATACTCATTTTCTAGCGAATCATACTAGGTAAAAGTCAATCTGTATATGTCGAAACACGATGA
TCATGCAAAGGAGGGATTCT

Downstream 100 bases:

>100_bases
AAACAACGGCTTAAACGCCGTTGTTTATCGTCTGCATTGCTTCACGTTTTTTTAATACAAATAATTCTAAACGGTCTAGG
CCTTCTCTCAGCGTGTCCAT

Product: sporulation-specific ATP-dependent protein histidine kinase

Products: NA

Alternate protein names: Stage II sporulation protein F; Stage II sporulation protein J

Number of amino acids: Translated: 606; Mature: 606

Protein sequence:

>606_residues
MEQDTQHVKPLQTKTDIHAVLASNGRIIYISANSKLHLGYLQGEMIGSFLKTFLHEEDQFLVESYFYNEHHLMPCTFRFI
KKDHTIVWVEAAVEIVTTRAERTEREIILKMKVLEEETGHQSLNCEKHEIEPASPESTTYITDDYERLVENLPSPLCISV
KGKIVYVNSAMLSMLGAKSKDAIIGKSSYEFIEEEYHDIVKNRIIRMQKGMEVGMIEQTWKRLDGTPVHLEVKASPTVYK
NQQAELLLLIDISSRKKFQTILQKSRERYQLLIQNSIDTIAVIHNGKWVFMNESGISLFEAATYEDLIGKNIYDQLHPCD
HEDVKERIQNIAEQKTESEIVKQSWFTFQNRVIYTEMVCIPTTFFGEAAVQVILRDISERKQTEELMLKSEKLSIAGQLA
AGIAHEIRNPLTAIKGFLQLMKPTMEGNEHYFDIVFSELSRIELILSELLMLAKPQQNAVKEYLNLKKLIGEVSALLETQ
ANLNGIFIRTSYEKDSIYINGDQNQLKQVFINLIKNAVESMPDGGTVDIIITEDEHSVHVTVKDEGEGIPEKVLNRIGEP
FLTTKEKGTGLGLMVTFNIIENHQGVIHVDSHPEKGTAFKISFPKK

Sequences:

>Translated_606_residues
MEQDTQHVKPLQTKTDIHAVLASNGRIIYISANSKLHLGYLQGEMIGSFLKTFLHEEDQFLVESYFYNEHHLMPCTFRFI
KKDHTIVWVEAAVEIVTTRAERTEREIILKMKVLEEETGHQSLNCEKHEIEPASPESTTYITDDYERLVENLPSPLCISV
KGKIVYVNSAMLSMLGAKSKDAIIGKSSYEFIEEEYHDIVKNRIIRMQKGMEVGMIEQTWKRLDGTPVHLEVKASPTVYK
NQQAELLLLIDISSRKKFQTILQKSRERYQLLIQNSIDTIAVIHNGKWVFMNESGISLFEAATYEDLIGKNIYDQLHPCD
HEDVKERIQNIAEQKTESEIVKQSWFTFQNRVIYTEMVCIPTTFFGEAAVQVILRDISERKQTEELMLKSEKLSIAGQLA
AGIAHEIRNPLTAIKGFLQLMKPTMEGNEHYFDIVFSELSRIELILSELLMLAKPQQNAVKEYLNLKKLIGEVSALLETQ
ANLNGIFIRTSYEKDSIYINGDQNQLKQVFINLIKNAVESMPDGGTVDIIITEDEHSVHVTVKDEGEGIPEKVLNRIGEP
FLTTKEKGTGLGLMVTFNIIENHQGVIHVDSHPEKGTAFKISFPKK
>Mature_606_residues
MEQDTQHVKPLQTKTDIHAVLASNGRIIYISANSKLHLGYLQGEMIGSFLKTFLHEEDQFLVESYFYNEHHLMPCTFRFI
KKDHTIVWVEAAVEIVTTRAERTEREIILKMKVLEEETGHQSLNCEKHEIEPASPESTTYITDDYERLVENLPSPLCISV
KGKIVYVNSAMLSMLGAKSKDAIIGKSSYEFIEEEYHDIVKNRIIRMQKGMEVGMIEQTWKRLDGTPVHLEVKASPTVYK
NQQAELLLLIDISSRKKFQTILQKSRERYQLLIQNSIDTIAVIHNGKWVFMNESGISLFEAATYEDLIGKNIYDQLHPCD
HEDVKERIQNIAEQKTESEIVKQSWFTFQNRVIYTEMVCIPTTFFGEAAVQVILRDISERKQTEELMLKSEKLSIAGQLA
AGIAHEIRNPLTAIKGFLQLMKPTMEGNEHYFDIVFSELSRIELILSELLMLAKPQQNAVKEYLNLKKLIGEVSALLETQ
ANLNGIFIRTSYEKDSIYINGDQNQLKQVFINLIKNAVESMPDGGTVDIIITEDEHSVHVTVKDEGEGIPEKVLNRIGEP
FLTTKEKGTGLGLMVTFNIIENHQGVIHVDSHPEKGTAFKISFPKK

Specific function: Phosphorylates the sporulation-regulatory proteins spo0A and spo0F. It also autophosphorylates in the presence of ATP

COG id: COG2202

COG function: function code T; FOG: PAS/PAC domain

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 PAS (PER-ARNT-SIM) domains

Homologues:

Organism=Escherichia coli, GI1788549, Length=349, Percent_Identity=30.0859598853868, Blast_Score=145, Evalue=9e-36,
Organism=Escherichia coli, GI1790436, Length=233, Percent_Identity=36.480686695279, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1790300, Length=237, Percent_Identity=29.1139240506329, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1786783, Length=218, Percent_Identity=29.3577981651376, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1786600, Length=217, Percent_Identity=27.1889400921659, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI145693157, Length=272, Percent_Identity=26.8382352941176, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788713, Length=232, Percent_Identity=25.8620689655172, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1788393, Length=214, Percent_Identity=25.2336448598131, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1789149, Length=250, Percent_Identity=24.8, Blast_Score=76, Evalue=7e-15,
Organism=Escherichia coli, GI1790346, Length=228, Percent_Identity=25.4385964912281, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1786912, Length=236, Percent_Identity=25.8474576271186, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI48994928, Length=244, Percent_Identity=25.8196721311475, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1790551, Length=218, Percent_Identity=27.0642201834862, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI87081816, Length=216, Percent_Identity=25.9259259259259, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790861, Length=221, Percent_Identity=24.4343891402715, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KINA_BACSU (P16497)

Other databases:

- EMBL:   M29450
- EMBL:   M31067
- EMBL:   AJ222587
- EMBL:   AL009126
- PIR:   A33496
- RefSeq:   NP_389282.1
- PDB:   2VLG
- PDBsum:   2VLG
- ProteinModelPortal:   P16497
- EnsemblBacteria:   EBBACT00000003684
- GeneID:   939230
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU13990
- NMPDR:   fig|224308.1.peg.1401
- GenoList:   BSU13990
- GeneTree:   EBGT00070000031947
- HOGENOM:   HBG337961
- OMA:   QNSIDTI
- PhylomeDB:   P16497
- ProtClustDB:   CLSK887211
- BioCyc:   BSUB:BSU13990-MONOMER
- BRENDA:   2.7.13.3
- GO:   GO:0016020
- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR013655
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- Gene3D:   G3DSA:3.30.565.10
- PRINTS:   PR00344
- SMART:   SM00387
- SMART:   SM00388
- SMART:   SM00086
- SMART:   SM00091
- TIGRFAMs:   TIGR00229

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS; PF08447 PAS_3; SSF55874 ATP_bd_ATPase; SSF47384 His_kin_homodim

EC number: =2.7.13.3

Molecular weight: Translated: 69172; Mature: 69172

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS50109 HIS_KIN; PS50113 PAC; PS50112 PAS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQDTQHVKPLQTKTDIHAVLASNGRIIYISANSKLHLGYLQGEMIGSFLKTFLHEEDQF
CCCCCCCCCCCCCHHHHHHEEECCCEEEEEECCCEEEEEEHHHHHHHHHHHHHHCCCCHH
LVESYFYNEHHLMPCTFRFIKKDHTIVWVEAAVEIVTTRAERTEREIILKMKVLEEETGH
HHHHHCCCCCCCCHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QSLNCEKHEIEPASPESTTYITDDYERLVENLPSPLCISVKGKIVYVNSAMLSMLGAKSK
CCCCCCHHCCCCCCCCCCEEECCHHHHHHHHCCCCEEEEECCEEEEECHHHHHHHCCCCC
DAIIGKSSYEFIEEEYHDIVKNRIIRMQKGMEVGMIEQTWKRLDGTPVHLEVKASPTVYK
CCEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEEECCCCEEC
NQQAELLLLIDISSRKKFQTILQKSRERYQLLIQNSIDTIAVIHNGKWVFMNESGISLFE
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEECCCCCHHH
AATYEDLIGKNIYDQLHPCDHEDVKERIQNIAEQKTESEIVKQSWFTFQNRVIYTEMVCI
HHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEE
PTTFFGEAAVQVILRDISERKQTEELMLKSEKLSIAGQLAAGIAHEIRNPLTAIKGFLQL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKPTMEGNEHYFDIVFSELSRIELILSELLMLAKPQQNAVKEYLNLKKLIGEVSALLETQ
HHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
ANLNGIFIRTSYEKDSIYINGDQNQLKQVFINLIKNAVESMPDGGTVDIIITEDEHSVHV
CCCCEEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEE
TVKDEGEGIPEKVLNRIGEPFLTTKEKGTGLGLMVTFNIIENHQGVIHVDSHPEKGTAFK
EECCCCCCCHHHHHHHCCCCCEECCCCCCCEEEEEEEEEECCCCCEEEECCCCCCCCEEE
ISFPKK
EECCCC
>Mature Secondary Structure
MEQDTQHVKPLQTKTDIHAVLASNGRIIYISANSKLHLGYLQGEMIGSFLKTFLHEEDQF
CCCCCCCCCCCCCHHHHHHEEECCCEEEEEECCCEEEEEEHHHHHHHHHHHHHHCCCCHH
LVESYFYNEHHLMPCTFRFIKKDHTIVWVEAAVEIVTTRAERTEREIILKMKVLEEETGH
HHHHHCCCCCCCCHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QSLNCEKHEIEPASPESTTYITDDYERLVENLPSPLCISVKGKIVYVNSAMLSMLGAKSK
CCCCCCHHCCCCCCCCCCEEECCHHHHHHHHCCCCEEEEECCEEEEECHHHHHHHCCCCC
DAIIGKSSYEFIEEEYHDIVKNRIIRMQKGMEVGMIEQTWKRLDGTPVHLEVKASPTVYK
CCEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEEECCCCEEC
NQQAELLLLIDISSRKKFQTILQKSRERYQLLIQNSIDTIAVIHNGKWVFMNESGISLFE
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEECCCCCHHH
AATYEDLIGKNIYDQLHPCDHEDVKERIQNIAEQKTESEIVKQSWFTFQNRVIYTEMVCI
HHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEE
PTTFFGEAAVQVILRDISERKQTEELMLKSEKLSIAGQLAAGIAHEIRNPLTAIKGFLQL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKPTMEGNEHYFDIVFSELSRIELILSELLMLAKPQQNAVKEYLNLKKLIGEVSALLETQ
HHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
ANLNGIFIRTSYEKDSIYINGDQNQLKQVFINLIKNAVESMPDGGTVDIIITEDEHSVHV
CCCCEEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEE
TVKDEGEGIPEKVLNRIGEPFLTTKEKGTGLGLMVTFNIIENHQGVIHVDSHPEKGTAFK
EECCCCCCCHHHHHHHCCCCCEECCCCCCCEEEEEEEEEECCCCCEEEECCCCCCCCEEE
ISFPKK
EECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2104615; 2509430; 9384377