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The map label for this gene is ispA

Identifier: 16078384

GI number: 16078384

Start: 1386024

End: 1386983

Strand: Reverse

Name: ispA

Synonym: BSU13190

Alternate gene names: 16078384

Gene position: 1386983-1386024 (Counterclockwise)

Preceding gene: 16078386

Following gene: 255767311

Centisome position: 32.9

GC content: 47.08

Gene sequence:

>960_bases
ATGAATGGTGAAATCCGCTTGATCCCGTATGTGACGAATGAGCAGATCATGGATGTAAACGAGCTTCCTGAGGGCATTAA
AGTGATTAAGGCGCCAGAAATGTGGGCAAAAGGGGTAAAAGGCAAAAATATTAAAGTTGCTGTATTAGACACAGGCTGCG
ACACAAGCCACCCTGATTTAAAAAATCAAATCATCGGCGGAAAGAACTTCACGGATGACGACGGCGGCAAGGAAGATGCG
ATTTCCGACTACAACGGACACGGCACACACGTCGCCGGAACAATTGCAGCTAATGATTCAAACGGAGGCATTGCCGGAGT
CGCGCCTGAGGCAAGCCTATTGATTGTGAAGGTTCTTGGCGGCGAGAACGGCAGCGGACAATACGAATGGATAATTAACG
GCATCAACTACGCGGTGGAACAGAAAGTCGACATCATCTCAATGTCCCTTGGAGGGCCAAGCGATGTGCCAGAGCTAAAA
GAAGCAGTGAAAAACGCAGTGAAAAACGGAGTGCTTGTCGTTTGTGCAGCGGGAAATGAAGGTGACGGCGACGAACGCAC
AGAAGAGCTTTCCTACCCCGCAGCTTATAATGAAGTGATTGCAGTTGGATCTGTTTCTGTAGCGCGAGAATTATCAGAAT
TTTCTAACGCGAATAAAGAGATTGACCTTGTGGCACCAGGAGAAAACATCTTATCCACCCTTCCCAACAAGAAGTACGGT
AAGCTGACCGGCACTTCAATGGCTGCCCCTCATGTCAGCGGTGCGCTTGCTTTAATCAAAAGCTATGAAGAAGAATCATT
TCAAAGAAAGCTTTCTGAATCTGAGGTTTTCGCACAGCTAATCCGCAGGACACTTCCTCTTGATATTGCAAAAACGCTGG
CAGGCAATGGATTCCTGTATTTAACAGCTCCTGATGAGCTCGCAGAAAAAGCAGAGCAATCACATTTGTTGACCCTATAA

Upstream 100 bases:

>100_bases
AAATGCTTCAAATGGCTCTGTCCGAGCGCTTGCTTTTTTCATATAATATGAGGCAACACCCTTGAATCCACTTGCAAGCA
TAAAAAAGGAGGGCTTTTTT

Downstream 100 bases:

>100_bases
GATTATTTTTCTTATATAATATACACCACATCATGTAAATAAAAATTTCAAATTCTATGTTGACAATGAATTTGAATTAC
TGTTAAGATTACCAACAAAT

Product: intracellular serine protease

Products: NA

Alternate protein names: ISP-1

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MNGEIRLIPYVTNEQIMDVNELPEGIKVIKAPEMWAKGVKGKNIKVAVLDTGCDTSHPDLKNQIIGGKNFTDDDGGKEDA
ISDYNGHGTHVAGTIAANDSNGGIAGVAPEASLLIVKVLGGENGSGQYEWIINGINYAVEQKVDIISMSLGGPSDVPELK
EAVKNAVKNGVLVVCAAGNEGDGDERTEELSYPAAYNEVIAVGSVSVARELSEFSNANKEIDLVAPGENILSTLPNKKYG
KLTGTSMAAPHVSGALALIKSYEEESFQRKLSESEVFAQLIRRTLPLDIAKTLAGNGFLYLTAPDELAEKAEQSHLLTL

Sequences:

>Translated_319_residues
MNGEIRLIPYVTNEQIMDVNELPEGIKVIKAPEMWAKGVKGKNIKVAVLDTGCDTSHPDLKNQIIGGKNFTDDDGGKEDA
ISDYNGHGTHVAGTIAANDSNGGIAGVAPEASLLIVKVLGGENGSGQYEWIINGINYAVEQKVDIISMSLGGPSDVPELK
EAVKNAVKNGVLVVCAAGNEGDGDERTEELSYPAAYNEVIAVGSVSVARELSEFSNANKEIDLVAPGENILSTLPNKKYG
KLTGTSMAAPHVSGALALIKSYEEESFQRKLSESEVFAQLIRRTLPLDIAKTLAGNGFLYLTAPDELAEKAEQSHLLTL
>Mature_319_residues
MNGEIRLIPYVTNEQIMDVNELPEGIKVIKAPEMWAKGVKGKNIKVAVLDTGCDTSHPDLKNQIIGGKNFTDDDGGKEDA
ISDYNGHGTHVAGTIAANDSNGGIAGVAPEASLLIVKVLGGENGSGQYEWIINGINYAVEQKVDIISMSLGGPSDVPELK
EAVKNAVKNGVLVVCAAGNEGDGDERTEELSYPAAYNEVIAVGSVSVARELSEFSNANKEIDLVAPGENILSTLPNKKYG
KLTGTSMAAPHVSGALALIKSYEEESFQRKLSESEVFAQLIRRTLPLDIAKTLAGNGFLYLTAPDELAEKAEQSHLLTL

Specific function: Major intracellular protease produced by Bacillus subtilis

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family

Homologues:

Organism=Homo sapiens, GI4506775, Length=249, Percent_Identity=32.1285140562249, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI31317307, Length=294, Percent_Identity=27.5510204081633, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6320775, Length=334, Percent_Identity=32.6347305389222, Blast_Score=116, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6324576, Length=237, Percent_Identity=34.5991561181435, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6319893, Length=263, Percent_Identity=32.319391634981, Blast_Score=94, Evalue=3e-20,
Organism=Drosophila melanogaster, GI45550681, Length=248, Percent_Identity=32.258064516129, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI17136192, Length=259, Percent_Identity=30.1158301158301, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISP1_BACSU (P11018)

Other databases:

- EMBL:   M13760
- EMBL:   AJ002571
- EMBL:   AL009126
- PIR:   I39866
- RefSeq:   NP_389202.1
- ProteinModelPortal:   P11018
- SMR:   P11018
- MEROPS:   S08.030
- EnsemblBacteria:   EBBACT00000004059
- GeneID:   939934
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU13190
- NMPDR:   fig|224308.1.peg.1321
- GenoList:   BSU13190
- GeneTree:   EBGT00050000000194
- HOGENOM:   HBG752219
- OMA:   LDTGCDT
- ProtClustDB:   CLSK873248
- BioCyc:   BSUB:BSU13190-MONOMER
- GO:   GO:0005737
- GO:   GO:0006508
- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500
- Gene3D:   G3DSA:3.40.50.200
- PANTHER:   PTHR10795
- PRINTS:   PR00723

Pfam domain/function: PF00082 Peptidase_S8; SSF52743 Pept_S8_S53

EC number: NA

Molecular weight: Translated: 33849; Mature: 33849

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS00136 SUBTILASE_ASP; PS00137 SUBTILASE_HIS; PS00138 SUBTILASE_SER

Important sites: ACT_SITE 50-50 ACT_SITE 87-87 ACT_SITE 246-246

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGEIRLIPYVTNEQIMDVNELPEGIKVIKAPEMWAKGVKGKNIKVAVLDTGCDTSHPDL
CCCCEEEEEEECCCCCCCHHHCCCCCEEEECCHHHHCCCCCCCEEEEEEECCCCCCCCCH
KNQIIGGKNFTDDDGGKEDAISDYNGHGTHVAGTIAANDSNGGIAGVAPEASLLIVKVLG
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEECCCCEEEEEEEEC
GENGSGQYEWIINGINYAVEQKVDIISMSLGGPSDVPELKEAVKNAVKNGVLVVCAAGNE
CCCCCCEEEEEEECCCHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCC
GDGDERTEELSYPAAYNEVIAVGSVSVARELSEFSNANKEIDLVAPGENILSTLPNKKYG
CCCCHHHHHCCCCCHHHHEEEECCHHHHHHHHHHHCCCCEEEEEECCHHHHHHCCCCCCC
KLTGTSMAAPHVSGALALIKSYEEESFQRKLSESEVFAQLIRRTLPLDIAKTLAGNGFLY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEE
LTAPDELAEKAEQSHLLTL
EECCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNGEIRLIPYVTNEQIMDVNELPEGIKVIKAPEMWAKGVKGKNIKVAVLDTGCDTSHPDL
CCCCEEEEEEECCCCCCCHHHCCCCCEEEECCHHHHCCCCCCCEEEEEEECCCCCCCCCH
KNQIIGGKNFTDDDGGKEDAISDYNGHGTHVAGTIAANDSNGGIAGVAPEASLLIVKVLG
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEECCCCEEEEEEEEC
GENGSGQYEWIINGINYAVEQKVDIISMSLGGPSDVPELKEAVKNAVKNGVLVVCAAGNE
CCCCCCEEEEEEECCCHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCC
GDGDERTEELSYPAAYNEVIAVGSVSVARELSEFSNANKEIDLVAPGENILSTLPNKKYG
CCCCHHHHHCCCCCHHHHEEEECCHHHHHHHHHHHCCCCEEEEEECCHHHHHHCCCCCCC
KLTGTSMAAPHVSGALALIKSYEEESFQRKLSESEVFAQLIRRTLPLDIAKTLAGNGFLY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEE
LTAPDELAEKAEQSHLLTL
EECCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3087947; 9384377