| Definition | Bacillus subtilis subsp. subtilis str. 168 chromosome, complete genome. |
|---|---|
| Accession | NC_000964 |
| Length | 4,215,606 |
Click here to switch to the map view.
The map label for this gene is yjmF
Identifier: 16078300
GI number: 16078300
Start: 1306562
End: 1307398
Strand: Direct
Name: yjmF
Synonym: BSU12350
Alternate gene names: 16078300
Gene position: 1306562-1307398 (Clockwise)
Preceding gene: 16078299
Following gene: 16078301
Centisome position: 30.99
GC content: 52.09
Gene sequence:
>837_bases ATGATCCCGCTGCATGAGAACCTGGCTGGTAAAACGGCTGTCATCACTGGCGGCAGCGGCGTGCTTTGCTCTGCGATGGC CCGGGAGCTAGCCCGTCATGGCATGAAGGTGGCGATTTTGAATCGGACGGCTGAAAAAGGCCAAGCGGTCGTGAAGGAGA TAACGGCGGCTGGCGGCACAGCGTGCGCTGTTGCTGCGGATGTGCTGGACAGGATGTCACTGGAGCGGGCAAAGGAAGAC ATCCTTGGCCAATTTGGCGCTGTTGATCTGTTAATTAACGGGGCTGGCGGCAATCATCCTGACGCGATAACCGATGTGGA GACATATGAAGAAGCGGGAGAAGGCCAATCCTTTTTTGATATGGATGAGAGGGGCTTTCTAACTGTATTCTCCACCAACT TCACCGGTGCGTTTCTGGCCTCGCAAGTGTTTGGTAAAGAACTGCTGAAGGCGGATTCACCCGCGATCATCAACCTTTCT TCCATGAGTGCTTATTCACCTATGACGAAGGTTCCGGCATACAGTGCTGCGAAAGCATCCATCAATAATTTTACGATGTG GATGGCTGTTCATTTTGCCGAAACCGGGCTGCGGGTCAATGCGATTGCCCCAGGCTTCTTTCTGACAAAACAAAATCATG ATCTGCTGATCAACCAAGACGGAACGTTCACCAGCCGATCTCACAAAATTATTGCGGGAACACCGATGAAGCGCTTCGGA AAACCGGAGGATTTGCTCGGTACGCTCCTTTGGCTGGCGGATGAATCCTATTCCGGTTTTGTCACTGGGATCACCGTTCC TGTCGATGGAGGATTTATGGCTTATTCAGGTGTGTAA
Upstream 100 bases:
>100_bases GCCGCCCGGGATATGGCTTATACGATCGGGCACTTGGCATCATGTATTTGAACGGGCTGTGGGACGCTTATGAAGCAATG GCAAAAAAAGAGGTGGGCAT
Downstream 100 bases:
>100_bases CGAAAGCGGTGCAACAGAAACTGAACCAACATAGGGGTGAGGTTGAGATGTTTTCAAAAGATAAGCTTCCCGTTATCCTT TTTTTGTTCCTGGCAGGGGT
Product: D-mannonate oxidoreductase
Products: D-Gluconate; Nicotinamide adenine dinucleotide phosphate [C]
Alternate protein names: NA
Number of amino acids: Translated: 278; Mature: 278
Protein sequence:
>278_residues MIPLHENLAGKTAVITGGSGVLCSAMARELARHGMKVAILNRTAEKGQAVVKEITAAGGTACAVAADVLDRMSLERAKED ILGQFGAVDLLINGAGGNHPDAITDVETYEEAGEGQSFFDMDERGFLTVFSTNFTGAFLASQVFGKELLKADSPAIINLS SMSAYSPMTKVPAYSAAKASINNFTMWMAVHFAETGLRVNAIAPGFFLTKQNHDLLINQDGTFTSRSHKIIAGTPMKRFG KPEDLLGTLLWLADESYSGFVTGITVPVDGGFMAYSGV
Sequences:
>Translated_278_residues MIPLHENLAGKTAVITGGSGVLCSAMARELARHGMKVAILNRTAEKGQAVVKEITAAGGTACAVAADVLDRMSLERAKED ILGQFGAVDLLINGAGGNHPDAITDVETYEEAGEGQSFFDMDERGFLTVFSTNFTGAFLASQVFGKELLKADSPAIINLS SMSAYSPMTKVPAYSAAKASINNFTMWMAVHFAETGLRVNAIAPGFFLTKQNHDLLINQDGTFTSRSHKIIAGTPMKRFG KPEDLLGTLLWLADESYSGFVTGITVPVDGGFMAYSGV >Mature_278_residues MIPLHENLAGKTAVITGGSGVLCSAMARELARHGMKVAILNRTAEKGQAVVKEITAAGGTACAVAADVLDRMSLERAKED ILGQFGAVDLLINGAGGNHPDAITDVETYEEAGEGQSFFDMDERGFLTVFSTNFTGAFLASQVFGKELLKADSPAIINLS SMSAYSPMTKVPAYSAAKASINNFTMWMAVHFAETGLRVNAIAPGFFLTKQNHDLLINQDGTFTSRSHKIIAGTPMKRFG KPEDLLGTLLWLADESYSGFVTGITVPVDGGFMAYSGV
Specific function: Catalyzes A Reversible Reduction Of 5-Ketoglutanate To Form D-Gluconate. Dependent On Nadp, Almost Inactive With NAD. [C]
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family
Homologues:
Organism=Homo sapiens, GI40254992, Length=262, Percent_Identity=24.0458015267176, Blast_Score=87, Evalue=1e-17, Organism=Homo sapiens, GI19923817, Length=275, Percent_Identity=25.0909090909091, Blast_Score=85, Evalue=6e-17, Organism=Homo sapiens, GI7705925, Length=270, Percent_Identity=25.9259259259259, Blast_Score=85, Evalue=7e-17, Organism=Homo sapiens, GI32483357, Length=271, Percent_Identity=25.4612546125461, Blast_Score=80, Evalue=3e-15, Organism=Homo sapiens, GI10190704, Length=265, Percent_Identity=26.7924528301887, Blast_Score=78, Evalue=8e-15, Organism=Homo sapiens, GI40254986, Length=259, Percent_Identity=27.027027027027, Blast_Score=72, Evalue=8e-13, Organism=Homo sapiens, GI31542939, Length=211, Percent_Identity=28.9099526066351, Blast_Score=69, Evalue=3e-12, Organism=Homo sapiens, GI15277342, Length=277, Percent_Identity=26.3537906137184, Blast_Score=69, Evalue=6e-12, Organism=Homo sapiens, GI59889578, Length=245, Percent_Identity=25.3061224489796, Blast_Score=69, Evalue=7e-12, Organism=Homo sapiens, GI4758504, Length=212, Percent_Identity=30.188679245283, Blast_Score=68, Evalue=1e-11, Organism=Escherichia coli, GI1790717, Length=269, Percent_Identity=28.996282527881, Blast_Score=114, Evalue=7e-27, Organism=Escherichia coli, GI1789208, Length=269, Percent_Identity=29.7397769516729, Blast_Score=111, Evalue=4e-26, Organism=Escherichia coli, GI1788459, Length=267, Percent_Identity=27.7153558052434, Blast_Score=99, Evalue=3e-22, Organism=Escherichia coli, GI1787905, Length=264, Percent_Identity=28.4090909090909, Blast_Score=95, Evalue=6e-21, Organism=Escherichia coli, GI87082160, Length=277, Percent_Identity=29.2418772563177, Blast_Score=94, Evalue=8e-21, Organism=Escherichia coli, GI87082100, Length=279, Percent_Identity=29.3906810035842, Blast_Score=90, Evalue=2e-19, Organism=Escherichia coli, GI1787335, Length=266, Percent_Identity=27.8195488721804, Blast_Score=89, Evalue=3e-19, Organism=Escherichia coli, GI2367175, Length=272, Percent_Identity=29.4117647058824, Blast_Score=85, Evalue=4e-18, Organism=Escherichia coli, GI1789378, Length=267, Percent_Identity=30.3370786516854, Blast_Score=82, Evalue=4e-17, Organism=Escherichia coli, GI1789057, Length=272, Percent_Identity=27.5735294117647, Blast_Score=69, Evalue=5e-13, Organism=Escherichia coli, GI2367365, Length=265, Percent_Identity=27.9245283018868, Blast_Score=67, Evalue=1e-12, Organism=Escherichia coli, GI1786812, Length=208, Percent_Identity=24.5192307692308, Blast_Score=65, Evalue=4e-12, Organism=Caenorhabditis elegans, GI17563726, Length=277, Percent_Identity=29.2418772563177, Blast_Score=102, Evalue=2e-22, Organism=Caenorhabditis elegans, GI17559104, Length=270, Percent_Identity=30.3703703703704, Blast_Score=99, Evalue=2e-21, Organism=Caenorhabditis elegans, GI17560332, Length=269, Percent_Identity=28.996282527881, Blast_Score=97, Evalue=1e-20, Organism=Caenorhabditis elegans, GI17560220, Length=273, Percent_Identity=29.6703296703297, Blast_Score=96, Evalue=2e-20, Organism=Caenorhabditis elegans, GI17560150, Length=281, Percent_Identity=28.8256227758007, Blast_Score=96, Evalue=2e-20, Organism=Caenorhabditis elegans, GI72000259, Length=281, Percent_Identity=27.0462633451957, Blast_Score=95, Evalue=3e-20, Organism=Caenorhabditis elegans, GI17562990, Length=279, Percent_Identity=27.9569892473118, Blast_Score=95, Evalue=5e-20, Organism=Caenorhabditis elegans, GI17531453, Length=276, Percent_Identity=27.1739130434783, Blast_Score=90, Evalue=1e-18, Organism=Caenorhabditis elegans, GI17538486, Length=268, Percent_Identity=27.9850746268657, Blast_Score=90, Evalue=2e-18, Organism=Caenorhabditis elegans, GI17562906, Length=275, Percent_Identity=25.8181818181818, Blast_Score=89, Evalue=4e-18, Organism=Caenorhabditis elegans, GI17562910, Length=278, Percent_Identity=28.0575539568345, Blast_Score=88, Evalue=4e-18, Organism=Caenorhabditis elegans, GI25147288, Length=267, Percent_Identity=29.5880149812734, Blast_Score=87, Evalue=1e-17, Organism=Caenorhabditis elegans, GI17565030, Length=280, Percent_Identity=28.2142857142857, Blast_Score=85, Evalue=4e-17, Organism=Caenorhabditis elegans, GI17551412, Length=285, Percent_Identity=25.9649122807018, Blast_Score=84, Evalue=8e-17, Organism=Caenorhabditis elegans, GI115534694, Length=272, Percent_Identity=24.2647058823529, Blast_Score=84, Evalue=1e-16, Organism=Caenorhabditis elegans, GI17562908, Length=275, Percent_Identity=25.8181818181818, Blast_Score=81, Evalue=5e-16, Organism=Caenorhabditis elegans, GI17562904, Length=274, Percent_Identity=27.3722627737226, Blast_Score=81, Evalue=7e-16, Organism=Caenorhabditis elegans, GI17555706, Length=266, Percent_Identity=26.3157894736842, Blast_Score=81, Evalue=7e-16, Organism=Caenorhabditis elegans, GI17538480, Length=271, Percent_Identity=25.0922509225092, Blast_Score=78, Evalue=5e-15, Organism=Caenorhabditis elegans, GI71994604, Length=268, Percent_Identity=27.9850746268657, Blast_Score=77, Evalue=9e-15, Organism=Caenorhabditis elegans, GI17544670, Length=276, Percent_Identity=25.3623188405797, Blast_Score=77, Evalue=9e-15, Organism=Caenorhabditis elegans, GI193204405, Length=279, Percent_Identity=26.8817204301075, Blast_Score=76, Evalue=2e-14, Organism=Caenorhabditis elegans, GI17561402, Length=266, Percent_Identity=24.4360902255639, Blast_Score=75, Evalue=5e-14, Organism=Caenorhabditis elegans, GI17560676, Length=272, Percent_Identity=22.7941176470588, Blast_Score=72, Evalue=3e-13, Organism=Caenorhabditis elegans, GI71994600, Length=269, Percent_Identity=24.907063197026, Blast_Score=71, Evalue=8e-13, Organism=Caenorhabditis elegans, GI17536025, Length=267, Percent_Identity=25.8426966292135, Blast_Score=70, Evalue=2e-12, Organism=Caenorhabditis elegans, GI71982365, Length=235, Percent_Identity=25.531914893617, Blast_Score=64, Evalue=6e-11, Organism=Caenorhabditis elegans, GI17536651, Length=267, Percent_Identity=25.0936329588015, Blast_Score=64, Evalue=6e-11, Organism=Saccharomyces cerevisiae, GI6324126, Length=266, Percent_Identity=27.8195488721804, Blast_Score=87, Evalue=4e-18, Organism=Drosophila melanogaster, GI23397609, Length=265, Percent_Identity=29.811320754717, Blast_Score=105, Evalue=2e-23, Organism=Drosophila melanogaster, GI24639444, Length=268, Percent_Identity=29.8507462686567, Blast_Score=98, Evalue=7e-21, Organism=Drosophila melanogaster, GI21357041, Length=265, Percent_Identity=27.9245283018868, Blast_Score=94, Evalue=1e-19, Organism=Drosophila melanogaster, GI24644339, Length=276, Percent_Identity=27.1739130434783, Blast_Score=87, Evalue=9e-18, Organism=Drosophila melanogaster, GI28571526, Length=265, Percent_Identity=27.9245283018868, Blast_Score=87, Evalue=1e-17, Organism=Drosophila melanogaster, GI21355319, Length=273, Percent_Identity=26.7399267399267, Blast_Score=86, Evalue=2e-17, Organism=Drosophila melanogaster, GI21357673, Length=239, Percent_Identity=28.8702928870293, Blast_Score=81, Evalue=6e-16, Organism=Drosophila melanogaster, GI24644337, Length=268, Percent_Identity=27.2388059701493, Blast_Score=79, Evalue=4e-15, Organism=Drosophila melanogaster, GI24643142, Length=266, Percent_Identity=25.5639097744361, Blast_Score=76, Evalue=3e-14, Organism=Drosophila melanogaster, GI24580925, Length=206, Percent_Identity=28.6407766990291, Blast_Score=67, Evalue=2e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): UXUB_BACSU (O34896)
Other databases:
- EMBL: AF015825 - EMBL: AL009126 - PIR: H69852 - RefSeq: NP_389117.1 - HSSP: P50162 - ProteinModelPortal: O34896 - SMR: O34896 - EnsemblBacteria: EBBACT00000001994 - GeneID: 939418 - GenomeReviews: AL009126_GR - KEGG: bsu:BSU12350 - NMPDR: fig|224308.1.peg.1236 - GenoList: BSU12350 - GeneTree: EBGT00070000031994 - HOGENOM: HBG750976 - OMA: VLCSAMA - ProtClustDB: PRK08277 - BioCyc: BSUB:BSU12350-MONOMER - GO: GO:0005488 - InterPro: IPR002198 - InterPro: IPR002347 - InterPro: IPR016040 - InterPro: IPR020904 - Gene3D: G3DSA:3.40.50.720 - PANTHER: PTHR19410 - PRINTS: PR00081 - PRINTS: PR00080
Pfam domain/function: PF00106 adh_short
EC number: 1.1.1.69 [C]
Molecular weight: Translated: 29481; Mature: 29481
Theoretical pI: Translated: 5.32; Mature: 5.32
Prosite motif: PS00061 ADH_SHORT
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.7 %Cys (Translated Protein) 4.0 %Met (Translated Protein) 4.7 %Cys+Met (Translated Protein) 0.7 %Cys (Mature Protein) 4.0 %Met (Mature Protein) 4.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MIPLHENLAGKTAVITGGSGVLCSAMARELARHGMKVAILNRTAEKGQAVVKEITAAGGT CCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCC ACAVAADVLDRMSLERAKEDILGQFGAVDLLINGAGGNHPDAITDVETYEEAGEGQSFFD HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHCCCCCEEC MDERGFLTVFSTNFTGAFLASQVFGKELLKADSPAIINLSSMSAYSPMTKVPAYSAAKAS CCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHCCCCHHHHHC INNFTMWMAVHFAETGLRVNAIAPGFFLTKQNHDLLINQDGTFTSRSHKIIAGTPMKRFG CCCEEEEEEEEEHHCCEEEEEECCCEEEEECCCCEEEECCCCCCCCCCEEEECCCHHHHC KPEDLLGTLLWLADESYSGFVTGITVPVDGGFMAYSGV CCHHHHHHHHHHCCCCCCCEEEEEEEEECCCEEEECCC >Mature Secondary Structure MIPLHENLAGKTAVITGGSGVLCSAMARELARHGMKVAILNRTAEKGQAVVKEITAAGGT CCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCC ACAVAADVLDRMSLERAKEDILGQFGAVDLLINGAGGNHPDAITDVETYEEAGEGQSFFD HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHCCCCCEEC MDERGFLTVFSTNFTGAFLASQVFGKELLKADSPAIINLSSMSAYSPMTKVPAYSAAKAS CCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHCCCCHHHHHC INNFTMWMAVHFAETGLRVNAIAPGFFLTKQNHDLLINQDGTFTSRSHKIIAGTPMKRFG CCCEEEEEEEEEHHCCEEEEEECCCEEEEECCCCEEEECCCCCCCCCCEEEECCCHHHHC KPEDLLGTLLWLADESYSGFVTGITVPVDGGFMAYSGV CCHHHHHHHHHHCCCCCCCEEEEEEEEECCCEEEECCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NAD+; NADH; NADP+; NADPH [C]
Metal ions: Mn2+ [C]
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): 0.006 {NADPH}} 0.9 {5-keto-D-gluconate}} 0.02 {NADP+}} 20 {D-gluconate}} [C]
Substrates: 5-Dehydro-D-gluconate; H+; Nicotinamide adenine dinucleotide phosphate - reduced [C]
Specific reaction: 5-Dehydro-D-gluconate + H+ + Nicotinamide adenine dinucleotide phosphate - reduced <==> D-Gluconate + Nicotinamide adenine dinucleotide phosphate [C]
General reaction: Redox reaction [C]
Inhibitor: Cd2+; Cs2+; Cu2+; Divalent metal ions; Hg2+; Ni2+; p-Chloromercuribenzoate; Sn2+; Sulfhydryl reagents [C]
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 9579062; 9384377