Click here to switch to the map view.

The map label for this gene is yjmC

Identifier: 16078297

GI number: 16078297

Start: 1303423

End: 1304436

Strand: Direct

Name: yjmC

Synonym: BSU12320

Alternate gene names: 16078297

Gene position: 1303423-1304436 (Clockwise)

Preceding gene: 16078296

Following gene: 16078298

Centisome position: 30.92

GC content: 48.72

Gene sequence:

>1014_bases
TTGAAAACGATAACAATTGCAGCTGAAGAAGCAAAGGAACTCGTTTGGCAAAAGCTGGACGGTGCCGGTTTGAATGAACG
AGATGCTGAAAAAGTGGCAGATGTTCTCGTGCACGCTGATTTGCGCAATGTACATTCGCATGGCGTGCTGCACACAGAAC
ACTATGTGAACAGGCTTTTAGCGGGAGGGATCAATCCTGGGGCACAGCCTGTTTTTAAAGAGACGGGGCCTGTGACCGGG
GTGCTTGACGGAGACGATGGTTTCGGTCATGTGAATTGCGACATGGCGATGGACCATGCAATTGACATGGCGAAGAAAAA
AGGAGTCGGCATGGTCACGGCCGTAAACAGCAGCCATTGCGGAGCGCTAAGCTATTTTGTGCAAAAAGCGGCTGACGAAA
AGCTGATCGGAATGGCAATGACGCATACAGACAGTATCGTTGTCCCATTTGGGGGGAGGACTCCTATTTTAGGGACAAAT
CCGATTGCTTACGGAGTTCCGGCTAAGCATAAAAAACCGTTTATCCTAGATATGGCGACATCCAAAGTGGCTTTTGGGAA
GATTCTGCAGGCCCGTGAAGAGGGCAAAGAAATTCCTGAAGGATGGGGAGTCGATGAAAACGGAGAAGCAGTAACTGATC
CTGACAAGGTCGTCTCACTTTCAACATTCGGGGGCCCGAAAGGCTATGGACTATCGATTGTAGTGGATGTGTTTTCCGGA
TTGCTGGCGGGCGCGGCTTTTGGCCCTCATATTGCCAAAATGTACAACGGCCTTGATCAAAAAAGAAAGCTGGGGCATTA
CGTTTGCGCGATCAATCCATCCTTTTTTACTGACTGGGATACGTTTTTAGAGCAGATGGATGCCATGATTGATGAACTGC
AGCAATCACCGCCGGCTGTTGGATTCGAAAGAGTGTATGTGCCCGGCGAGATCGAGCAGCTGCATGAAGAAAGAAATAAG
AAAAACGGAATTTCTATCGCCCGGAGCGTGTATGAATTCTTAAAAAGCAGGTGA

Upstream 100 bases:

>100_bases
ATAATATAAAGAATATTTAAAATAATTTGTAAATAAAATGTGTTTGTAGGGGTTTCGCTTGGAGAGTGCCCAAAAAACTG
AAAAGGAAGGAGGCACTTGC

Downstream 100 bases:

>100_bases
GTAAAATGAAAGCGGTTCAAGTGCGAAAAGCGTATGATCTGGTGACAGCGGAGGTGAAGAAGCCAGTTCTTTCAAAGGAT
GATGAAGTGCTCGTGAAAGT

Product: oxidoreductase

Products: oxaloacetate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MKTITIAAEEAKELVWQKLDGAGLNERDAEKVADVLVHADLRNVHSHGVLHTEHYVNRLLAGGINPGAQPVFKETGPVTG
VLDGDDGFGHVNCDMAMDHAIDMAKKKGVGMVTAVNSSHCGALSYFVQKAADEKLIGMAMTHTDSIVVPFGGRTPILGTN
PIAYGVPAKHKKPFILDMATSKVAFGKILQAREEGKEIPEGWGVDENGEAVTDPDKVVSLSTFGGPKGYGLSIVVDVFSG
LLAGAAFGPHIAKMYNGLDQKRKLGHYVCAINPSFFTDWDTFLEQMDAMIDELQQSPPAVGFERVYVPGEIEQLHEERNK
KNGISIARSVYEFLKSR

Sequences:

>Translated_337_residues
MKTITIAAEEAKELVWQKLDGAGLNERDAEKVADVLVHADLRNVHSHGVLHTEHYVNRLLAGGINPGAQPVFKETGPVTG
VLDGDDGFGHVNCDMAMDHAIDMAKKKGVGMVTAVNSSHCGALSYFVQKAADEKLIGMAMTHTDSIVVPFGGRTPILGTN
PIAYGVPAKHKKPFILDMATSKVAFGKILQAREEGKEIPEGWGVDENGEAVTDPDKVVSLSTFGGPKGYGLSIVVDVFSG
LLAGAAFGPHIAKMYNGLDQKRKLGHYVCAINPSFFTDWDTFLEQMDAMIDELQQSPPAVGFERVYVPGEIEQLHEERNK
KNGISIARSVYEFLKSR
>Mature_337_residues
MKTITIAAEEAKELVWQKLDGAGLNERDAEKVADVLVHADLRNVHSHGVLHTEHYVNRLLAGGINPGAQPVFKETGPVTG
VLDGDDGFGHVNCDMAMDHAIDMAKKKGVGMVTAVNSSHCGALSYFVQKAADEKLIGMAMTHTDSIVVPFGGRTPILGTN
PIAYGVPAKHKKPFILDMATSKVAFGKILQAREEGKEIPEGWGVDENGEAVTDPDKVVSLSTFGGPKGYGLSIVVDVFSG
LLAGAAFGPHIAKMYNGLDQKRKLGHYVCAINPSFFTDWDTFLEQMDAMIDELQQSPPAVGFERVYVPGEIEQLHEERNK
KNGISIARSVYEFLKSR

Specific function: Involved In The Anaerobic Utilization Of Allantoin. [C]

COG id: COG2055

COG function: function code C; Malate/L-lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH2/MDH2 oxidoreductase family

Homologues:

Organism=Escherichia coli, GI1786727, Length=333, Percent_Identity=40.5405405405405, Blast_Score=268, Evalue=3e-73,
Organism=Escherichia coli, GI1787020, Length=313, Percent_Identity=32.2683706070287, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1790000, Length=338, Percent_Identity=24.8520710059172, Blast_Score=110, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17536633, Length=320, Percent_Identity=32.5, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17507179, Length=322, Percent_Identity=31.6770186335404, Blast_Score=157, Evalue=7e-39,
Organism=Drosophila melanogaster, GI24667904, Length=319, Percent_Identity=34.1692789968652, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24667908, Length=319, Percent_Identity=34.7962382445141, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI45553211, Length=319, Percent_Identity=34.7962382445141, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24667912, Length=319, Percent_Identity=34.7962382445141, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24658010, Length=316, Percent_Identity=29.746835443038, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24648360, Length=335, Percent_Identity=26.2686567164179, Blast_Score=119, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YJMC_BACSU (O34736)

Other databases:

- EMBL:   AF015825
- EMBL:   AL009126
- PIR:   E69852
- RefSeq:   NP_389114.1
- ProteinModelPortal:   O34736
- SMR:   O34736
- EnsemblBacteria:   EBBACT00000000565
- GeneID:   939828
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU12320
- NMPDR:   fig|224308.1.peg.1233
- GenoList:   BSU12320
- GeneTree:   EBGT00050000006140
- HOGENOM:   HBG553037
- OMA:   VDSHGVR
- PhylomeDB:   O34736
- ProtClustDB:   CLSK2460908
- BioCyc:   BSUB:BSU12320-MONOMER
- GO:   GO:0005737
- InterPro:   IPR003767
- PANTHER:   PTHR11091

Pfam domain/function: PF02615 Ldh_2; SSF89733 MDH_LDH

EC number: 1.1.1.37

Molecular weight: Translated: 36471; Mature: 36471

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTITIAAEEAKELVWQKLDGAGLNERDAEKVADVLVHADLRNVHSHGVLHTEHYVNRLL
CCEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AGGINPGAQPVFKETGPVTGVLDGDDGFGHVNCDMAMDHAIDMAKKKGVGMVTAVNSSHC
HCCCCCCCCCHHHCCCCEEEEEECCCCCCEEECCHHHHHHHHHHHHCCCEEEEEECCCCC
GALSYFVQKAADEKLIGMAMTHTDSIVVPFGGRTPILGTNPIAYGVPAKHKKPFILDMAT
HHHHHHHHHHCCCHHHEEEEECCCCEEEECCCCCCCCCCCCCEECCCCCCCCCEEEEECH
SKVAFGKILQAREEGKEIPEGWGVDENGEAVTDPDKVVSLSTFGGPKGYGLSIVVDVFSG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHEEEEECCCCCCCCCHHHHHHHHHH
LLAGAAFGPHIAKMYNGLDQKRKLGHYVCAINPSFFTDWDTFLEQMDAMIDELQQSPPAV
HHHHHHCCHHHHHHHCCHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCC
GFERVYVPGEIEQLHEERNKKNGISIARSVYEFLKSR
CCEEEECCCHHHHHHHHHCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKTITIAAEEAKELVWQKLDGAGLNERDAEKVADVLVHADLRNVHSHGVLHTEHYVNRLL
CCEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AGGINPGAQPVFKETGPVTGVLDGDDGFGHVNCDMAMDHAIDMAKKKGVGMVTAVNSSHC
HCCCCCCCCCHHHCCCCEEEEEECCCCCCEEECCHHHHHHHHHHHHCCCEEEEEECCCCC
GALSYFVQKAADEKLIGMAMTHTDSIVVPFGGRTPILGTNPIAYGVPAKHKKPFILDMAT
HHHHHHHHHHCCCHHHEEEEECCCCEEEECCCCCCCCCCCCCEECCCCCCCCCEEEEECH
SKVAFGKILQAREEGKEIPEGWGVDENGEAVTDPDKVVSLSTFGGPKGYGLSIVVDVFSG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHEEEEECCCCCCCCCHHHHHHHHHH
LLAGAAFGPHIAKMYNGLDQKRKLGHYVCAINPSFFTDWDTFLEQMDAMIDELQQSPPAV
HHHHHHCCHHHHHHHCCHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCC
GFERVYVPGEIEQLHEERNKKNGISIARSVYEFLKSR
CCEEEECCCHHHHHHHHHCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-malate; NAD+

Specific reaction: (S)-malate + NAD+ = oxaloacetate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579062; 9384377