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The map label for this gene is manA

Identifier: 16078267

GI number: 16078267

Start: 1274692

End: 1275639

Strand: Direct

Name: manA

Synonym: BSU12020

Alternate gene names: 16078267

Gene position: 1274692-1275639 (Clockwise)

Preceding gene: 255767271

Following gene: 16078268

Centisome position: 30.24

GC content: 43.46

Gene sequence:

>948_bases
ATGACGACTGAACCGTTATTTTTCAAGCCTGTTTTCAAAGAAAGAATTTGGGGCGGGACCGCTTTAGCTGATTTTGGCTA
TACCATTCCGTCACAACGAACAGGGGAGTGCTGGGCTTTTGCCGCGCATCAAAATGGTCAAAGCGTTGTTCAAAACGGAA
TGTATAAGGGGTTCACGCTCAGCGAATTATGGGAACATCACAGACATTTATTCGGACAGCTTGAAGGGGACCGTTTCCCT
CTGCTTACAAAAATATTAGATGCTGACCAGGACTTATCTGTTCAGGTGCATCCGAATGATGAATATGCCAACATACATGA
AAACGGTGAGCTTGGAAAAACAGAATGCTGGTACATTATTGATTGCCAAAAAGATGCCGAGATTATTTATGGCCACAATG
CAACAACAAAGGAAGAACTAACTACCATGATAGAGCGTGGAGAATGGGATGAGCTCTTGCGCCGTGTAAAGGTAAAGCCG
GGGGATTTTTTCTATGTGCCAAGCGGTACTGTTCATGCGATTGGAAAAGGAATTCTTGCTTTGGAGACGCAGCAGAACTC
AGACACAACCTACAGATTATATGATTATGACCGAAAAGATGCAGAAGGCAAGCTGCGCGAGCTTCATCTGAAAAAGAGCA
TTGAAGTGATAGAGGTCCCGTCTATTCCAGAACGGCATACAGTTCACCATGAACAAATTGAGGATTTGCTTACAACGACA
TTGATTGAATGCGCTTACTTTTCGGTGGGGAAATGGAACTTATCAGGATCAGCAAGCTTAAAGCAGCAAAAACCATTCCT
TCTTATCAGTGTGATTGAAGGGGAGGGCCGTATGATCTCTGGTGAGTATGTCTATCCTTTCAAAAAAGGAGATCATATGT
TGCTGCCTTACGGTCTTGGAGAATTTAAACTCGAAGGATATGCAGAATGTATCGTCTCCCATCTGTAA

Upstream 100 bases:

>100_bases
AGCTAATGGATGACAATTATAGGGAGCGGCTGCTCTCCGTGCAAACAACAGAAGAAGCATACAAGCTGCTAGAAGAAATC
GAATAAAGCGGGGGATTTTC

Downstream 100 bases:

>100_bases
CATGGCAGGGCTTGAGAAATAAATTTGCGAGTGAAGACGCCTATTCTCCAGCAATGATCTAAATAAAATAAGAGTTTAAT
AGTTGGAGAAGAAATGTTAC

Product: mannose-6 phosphate isomerase

Products: NA

Alternate protein names: Phosphohexomutase; Phosphomannose isomerase; PMI

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MTTEPLFFKPVFKERIWGGTALADFGYTIPSQRTGECWAFAAHQNGQSVVQNGMYKGFTLSELWEHHRHLFGQLEGDRFP
LLTKILDADQDLSVQVHPNDEYANIHENGELGKTECWYIIDCQKDAEIIYGHNATTKEELTTMIERGEWDELLRRVKVKP
GDFFYVPSGTVHAIGKGILALETQQNSDTTYRLYDYDRKDAEGKLRELHLKKSIEVIEVPSIPERHTVHHEQIEDLLTTT
LIECAYFSVGKWNLSGSASLKQQKPFLLISVIEGEGRMISGEYVYPFKKGDHMLLPYGLGEFKLEGYAECIVSHL

Sequences:

>Translated_315_residues
MTTEPLFFKPVFKERIWGGTALADFGYTIPSQRTGECWAFAAHQNGQSVVQNGMYKGFTLSELWEHHRHLFGQLEGDRFP
LLTKILDADQDLSVQVHPNDEYANIHENGELGKTECWYIIDCQKDAEIIYGHNATTKEELTTMIERGEWDELLRRVKVKP
GDFFYVPSGTVHAIGKGILALETQQNSDTTYRLYDYDRKDAEGKLRELHLKKSIEVIEVPSIPERHTVHHEQIEDLLTTT
LIECAYFSVGKWNLSGSASLKQQKPFLLISVIEGEGRMISGEYVYPFKKGDHMLLPYGLGEFKLEGYAECIVSHL
>Mature_314_residues
TTEPLFFKPVFKERIWGGTALADFGYTIPSQRTGECWAFAAHQNGQSVVQNGMYKGFTLSELWEHHRHLFGQLEGDRFPL
LTKILDADQDLSVQVHPNDEYANIHENGELGKTECWYIIDCQKDAEIIYGHNATTKEELTTMIERGEWDELLRRVKVKPG
DFFYVPSGTVHAIGKGILALETQQNSDTTYRLYDYDRKDAEGKLRELHLKKSIEVIEVPSIPERHTVHHEQIEDLLTTTL
IECAYFSVGKWNLSGSASLKQQKPFLLISVIEGEGRMISGEYVYPFKKGDHMLLPYGLGEFKLEGYAECIVSHL

Specific function: Unknown

COG id: COG1482

COG function: function code G; Phosphomannose isomerase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 1 family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MANA1_BACSU (O31646)

Other databases:

- EMBL:   AL009126
- PIR:   H69848
- RefSeq:   NP_389084.1
- HSSP:   P39841
- ProteinModelPortal:   O31646
- SMR:   O31646
- EnsemblBacteria:   EBBACT00000002133
- GeneID:   939393
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU12020
- NMPDR:   fig|224308.1.peg.1203
- GenoList:   BSU12020
- GeneTree:   EBGT00050000000824
- HOGENOM:   HBG729572
- OMA:   MTEPLFF
- ProtClustDB:   CLSK887088
- BioCyc:   BSUB:BSU12020-MONOMER
- InterPro:   IPR011051
- InterPro:   IPR001250
- InterPro:   IPR014628
- InterPro:   IPR014710
- Gene3D:   G3DSA:2.60.120.10
- PIRSF:   PIRSF036894
- TIGRFAMs:   TIGR00218

Pfam domain/function: PF01238 PMI_typeI; SSF51182 RmlC_like_cupin

EC number: =5.3.1.8

Molecular weight: Translated: 36003; Mature: 35872

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00965 PMI_I_1; PS00966 PMI_I_2

Important sites: ACT_SITE 192-192

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTEPLFFKPVFKERIWGGTALADFGYTIPSQRTGECWAFAAHQNGQSVVQNGMYKGFTL
CCCCCCEECHHHHHHHCCCCEEHHCCCCCCCCCCCCEEEEEECCCCHHHHHCCCCCCCCH
SELWEHHRHLFGQLEGDRFPLLTKILDADQDLSVQVHPNDEYANIHENGELGKTECWYII
HHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEE
DCQKDAEIIYGHNATTKEELTTMIERGEWDELLRRVKVKPGDFFYVPSGTVHAIGKGILA
ECCCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCEEEECCCCHHHHCCCEEE
LETQQNSDTTYRLYDYDRKDAEGKLRELHLKKSIEVIEVPSIPERHTVHHEQIEDLLTTT
EEECCCCCCEEEEEECCCCCCCCHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHH
LIECAYFSVGKWNLSGSASLKQQKPFLLISVIEGEGRMISGEYVYPFKKGDHMLLPYGLG
HHHHHHHCCEEECCCCCCCCCCCCCEEEEEEEECCCEEEECCEEEEEECCCEEEEECCCC
EFKLEGYAECIVSHL
CEEHHHHHHHHHHCC
>Mature Secondary Structure 
TTEPLFFKPVFKERIWGGTALADFGYTIPSQRTGECWAFAAHQNGQSVVQNGMYKGFTL
CCCCCEECHHHHHHHCCCCEEHHCCCCCCCCCCCCEEEEEECCCCHHHHHCCCCCCCCH
SELWEHHRHLFGQLEGDRFPLLTKILDADQDLSVQVHPNDEYANIHENGELGKTECWYII
HHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEE
DCQKDAEIIYGHNATTKEELTTMIERGEWDELLRRVKVKPGDFFYVPSGTVHAIGKGILA
ECCCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCEEEECCCCHHHHCCCEEE
LETQQNSDTTYRLYDYDRKDAEGKLRELHLKKSIEVIEVPSIPERHTVHHEQIEDLLTTT
EEECCCCCCEEEEEECCCCCCCCHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHH
LIECAYFSVGKWNLSGSASLKQQKPFLLISVIEGEGRMISGEYVYPFKKGDHMLLPYGLG
HHHHHHHCCEEECCCCCCCCCCCCCEEEEEEEECCCEEEECCEEEEEECCCEEEEECCCC
EFKLEGYAECIVSHL
CEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377