Definition Bacillus subtilis subsp. subtilis str. 168 chromosome, complete genome.
Accession NC_000964
Length 4,215,606

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The map label for this gene is yjcJ

Identifier: 16078253

GI number: 16078253

Start: 1259606

End: 1260778

Strand: Direct

Name: yjcJ

Synonym: BSU11880

Alternate gene names: 16078253

Gene position: 1259606-1260778 (Clockwise)

Preceding gene: 16078252

Following gene: 16078257

Centisome position: 29.88

GC content: 50.9

Gene sequence:

>1173_bases
TTGAGTAAACACAATTGGACGCTGGAAACCCAGCTCGTGCACAATCCATTTAAAACAGACGGCGGAACCGGGGCAGTCAG
TGTACCGATTCAGCACGCCTCGACTTTTCACCAATCTTCATTTGAAGAGTTCGGGGCATATGATTACAGCCGCTCTGGCA
CGCCGACAAGAACAGCTCTCGAAGAAACAATTGCCGCACTGGAAGGCGGAACAAGAGGATTTGCTTTCAGTTCAGGCATG
GCTGCCATCTCAACAGCATTTTTACTGCTGTCACAGGGTGATCACGTTCTTGTGACAGAAGATGTATACGGCGGAACCTT
CCGTATGGTGACAGAGGTGCTGACCCGGTTTGGCATTGAGCATACGTTTGTCGACATGACGGATCGTAATGAAGTGGCGC
GGAGCATCAAGCCGAACACAAAGGTCATTTATATGGAGACGCCATCAAATCCGACACTTGGGATTACGGATATTAAAGCA
GTTGTTCAGCTTGCAAAAGAAAACGGCTGCCTGACCTTTCTGGACAATACATTCATGACGCCGGCACTGCAGCGTCCGCT
TGATCTCGGCGTGGATATTGTACTTCACAGCGCGACAAAATTCTTAAGCGGACACAGCGATGTGCTCTCAGGTTTGGCTG
CTGTCAAAGATGAAGAGCTTGGGAAGCAGCTGTATAAGCTACAAAACGCGTTCGGCGCCGTCCTTGGCGTGCAAGATTGC
TGGCTTGTCCTGCGCGGATTAAAAACATTGCAGGTCCGTTTAGAGAAAGCGAGTCAAACAGCTCAGCGGCTTGCGGAATT
TTTCCAGAAGCACCCGGCGGTCAAGCGCGTGTATTATCCGGGACTGGCTGATCATCCCGGCGCTGAAACCCATAAAAGCC
AGTCAACGGGAGCGGGCGCCGTGCTATCTTTTGAGCTTGAAAGCAAAGAGGCCGTTAAGAAGTTAGTCGAGAATGTGTCA
CTCCCAGTCTTCGCTGTCAGCCTTGGCGCCGTGGAATCGATTTTGTCCTATCCGGCAACGATGTCTCATGCGGCAATGCC
TAAGGAAGAGCGTGAAAAACGCGGTATCACTGATGGCCTGCTCCGTCTGAGTGTCGGCGTCGAACATGCAGACGATTTAG
AACACGATTTCGAACAAGCGCTGAAAGAAATTGCTCCAGTTTCGGTTCGTTAA

Upstream 100 bases:

>100_bases
AATCGGTTGCTGCGCTTTTCTGTCGGTATTGAACATGCGGAAGATTTAAAAGAGGATCTAAAACAGGCATTATGTCAGGT
CAAAGAGGGAGCTGTTTCAT

Downstream 100 bases:

>100_bases
AAAAGAAGCCGGGGATAGTCCGGCTTCTTTTATTATTGTTCGTCATCAGGATTCAGAATCGCGAGGACTTGATGATCCTC
CCAAACGCCATTAATTTTTA

Product: cystathionine beta-lyase

Products: NA

Alternate protein names: CBL; Beta-cystathionase; Cysteine lyase

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MSKHNWTLETQLVHNPFKTDGGTGAVSVPIQHASTFHQSSFEEFGAYDYSRSGTPTRTALEETIAALEGGTRGFAFSSGM
AAISTAFLLLSQGDHVLVTEDVYGGTFRMVTEVLTRFGIEHTFVDMTDRNEVARSIKPNTKVIYMETPSNPTLGITDIKA
VVQLAKENGCLTFLDNTFMTPALQRPLDLGVDIVLHSATKFLSGHSDVLSGLAAVKDEELGKQLYKLQNAFGAVLGVQDC
WLVLRGLKTLQVRLEKASQTAQRLAEFFQKHPAVKRVYYPGLADHPGAETHKSQSTGAGAVLSFELESKEAVKKLVENVS
LPVFAVSLGAVESILSYPATMSHAAMPKEEREKRGITDGLLRLSVGVEHADDLEHDFEQALKEIAPVSVR

Sequences:

>Translated_390_residues
MSKHNWTLETQLVHNPFKTDGGTGAVSVPIQHASTFHQSSFEEFGAYDYSRSGTPTRTALEETIAALEGGTRGFAFSSGM
AAISTAFLLLSQGDHVLVTEDVYGGTFRMVTEVLTRFGIEHTFVDMTDRNEVARSIKPNTKVIYMETPSNPTLGITDIKA
VVQLAKENGCLTFLDNTFMTPALQRPLDLGVDIVLHSATKFLSGHSDVLSGLAAVKDEELGKQLYKLQNAFGAVLGVQDC
WLVLRGLKTLQVRLEKASQTAQRLAEFFQKHPAVKRVYYPGLADHPGAETHKSQSTGAGAVLSFELESKEAVKKLVENVS
LPVFAVSLGAVESILSYPATMSHAAMPKEEREKRGITDGLLRLSVGVEHADDLEHDFEQALKEIAPVSVR
>Mature_389_residues
SKHNWTLETQLVHNPFKTDGGTGAVSVPIQHASTFHQSSFEEFGAYDYSRSGTPTRTALEETIAALEGGTRGFAFSSGMA
AISTAFLLLSQGDHVLVTEDVYGGTFRMVTEVLTRFGIEHTFVDMTDRNEVARSIKPNTKVIYMETPSNPTLGITDIKAV
VQLAKENGCLTFLDNTFMTPALQRPLDLGVDIVLHSATKFLSGHSDVLSGLAAVKDEELGKQLYKLQNAFGAVLGVQDCW
LVLRGLKTLQVRLEKASQTAQRLAEFFQKHPAVKRVYYPGLADHPGAETHKSQSTGAGAVLSFELESKEAVKKLVENVSL
PVFAVSLGAVESILSYPATMSHAAMPKEEREKRGITDGLLRLSVGVEHADDLEHDFEQALKEIAPVSVR

Specific function: Catalyzes the transformation of cystathionine into homocysteine. Also exhibits cysteine desulfhydrase activity in vitro, producing sulfide from cysteine

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family

Homologues:

Organism=Homo sapiens, GI21361334, Length=366, Percent_Identity=42.896174863388, Blast_Score=293, Evalue=2e-79,
Organism=Homo sapiens, GI299473759, Length=366, Percent_Identity=38.5245901639344, Blast_Score=244, Evalue=9e-65,
Organism=Homo sapiens, GI25453487, Length=365, Percent_Identity=38.6301369863014, Blast_Score=235, Evalue=5e-62,
Organism=Escherichia coli, GI1790375, Length=374, Percent_Identity=40.1069518716578, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI1789383, Length=393, Percent_Identity=31.2977099236641, Blast_Score=189, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17560056, Length=363, Percent_Identity=43.801652892562, Blast_Score=298, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI17538055, Length=362, Percent_Identity=42.2651933701657, Blast_Score=286, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI71980708, Length=370, Percent_Identity=39.4594594594595, Blast_Score=270, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6319307, Length=369, Percent_Identity=43.6314363143631, Blast_Score=292, Evalue=7e-80,
Organism=Saccharomyces cerevisiae, GI6321254, Length=412, Percent_Identity=33.9805825242718, Blast_Score=202, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6321170, Length=338, Percent_Identity=33.4319526627219, Blast_Score=179, Evalue=9e-46,
Organism=Saccharomyces cerevisiae, GI6323333, Length=389, Percent_Identity=32.133676092545, Blast_Score=159, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6321904, Length=397, Percent_Identity=30.4785894206549, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6322970, Length=343, Percent_Identity=24.7813411078717, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6323557, Length=348, Percent_Identity=23.2758620689655, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6322590, Length=371, Percent_Identity=23.989218328841, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19922554, Length=370, Percent_Identity=44.3243243243243, Blast_Score=304, Evalue=7e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): METC_BACSU (O31632)

Other databases:

- EMBL:   AL009126
- PIR:   B69847
- RefSeq:   NP_389070.1
- HSSP:   P53780
- ProteinModelPortal:   O31632
- SMR:   O31632
- EnsemblBacteria:   EBBACT00000001741
- GeneID:   936424
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU11880
- NMPDR:   fig|224308.1.peg.1189
- GenoList:   BSU11880
- GeneTree:   EBGT00070000031827
- HOGENOM:   HBG754729
- OMA:   DIRTVAK
- PhylomeDB:   O31632
- ProtClustDB:   PRK08064
- BioCyc:   BSUB:BSU11880-MONOMER
- GO:   GO:0005737
- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11808
- PIRSF:   PIRSF001434

Pfam domain/function: PF01053 Cys_Met_Meta_PP; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =4.4.1.8

Molecular weight: Translated: 42492; Mature: 42361

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MSKHNWTLETQLVHNPFKTDGGTGAVSVPIQHASTFHQSSFEEFGAYDYSRSGTPTRTAL
CCCCCCEEEEEEECCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
EETIAALEGGTRGFAFSSGMAAISTAFLLLSQGDHVLVTEDVYGGTFRMVTEVLTRFGIE
HHHHHHHCCCCCCCEECCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCC
HTFVDMTDRNEVARSIKPNTKVIYMETPSNPTLGITDIKAVVQLAKENGCLTFLDNTFMT
CEEEECCCHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCC
PALQRPLDLGVDIVLHSATKFLSGHSDVLSGLAAVKDEELGKQLYKLQNAFGAVLGVQDC
HHHHCCHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLVLRGLKTLQVRLEKASQTAQRLAEFFQKHPAVKRVYYPGLADHPGAETHKSQSTGAGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCCCCCE
VLSFELESKEAVKKLVENVSLPVFAVSLGAVESILSYPATMSHAAMPKEEREKRGITDGL
EEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHCCHHHHH
LRLSVGVEHADDLEHDFEQALKEIAPVSVR
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SKHNWTLETQLVHNPFKTDGGTGAVSVPIQHASTFHQSSFEEFGAYDYSRSGTPTRTAL
CCCCCEEEEEEECCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
EETIAALEGGTRGFAFSSGMAAISTAFLLLSQGDHVLVTEDVYGGTFRMVTEVLTRFGIE
HHHHHHHCCCCCCCEECCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCC
HTFVDMTDRNEVARSIKPNTKVIYMETPSNPTLGITDIKAVVQLAKENGCLTFLDNTFMT
CEEEECCCHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCC
PALQRPLDLGVDIVLHSATKFLSGHSDVLSGLAAVKDEELGKQLYKLQNAFGAVLGVQDC
HHHHCCHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLVLRGLKTLQVRLEKASQTAQRLAEFFQKHPAVKRVYYPGLADHPGAETHKSQSTGAGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCCCCCE
VLSFELESKEAVKKLVENVSLPVFAVSLGAVESILSYPATMSHAAMPKEEREKRGITDGL
EEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHCCHHHHH
LRLSVGVEHADDLEHDFEQALKEIAPVSVR
HHHHCCCCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377