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The map label for this gene is yisU

Identifier: 16078150

GI number: 16078150

Start: 1166008

End: 1166670

Strand: Reverse

Name: yisU

Synonym: BSU10870

Alternate gene names: 16078150

Gene position: 1166670-1166008 (Counterclockwise)

Preceding gene: 16078153

Following gene: 50812212

Centisome position: 27.68

GC content: 47.81

Gene sequence:

>663_bases
GTGACAATCTCTCTTATGATTGAAAAAAAATGTGAAAGGAGCTTTTTCAGCATGAATGCAATCATTCACGGAATTGTGCT
CGCGTTTGGCTTAATTTTGCCGCTGGGAGTGCAGAATGTGTTTATTTTTCAGCAAGGCGCGTTGCAAAAGCATATTTGGC
GGGCTCTCCCAGCTGTCATCAGTGCTTCAGTCTGCGATACCTTGTTAATTGTATTAGCCGTTGCGGGCGTGTCTGTCATT
GTGCAGGAGCTGCCTGTGTTTGAGACAGTCATGATGGCCGGCGGATTTTTATTTTTGCTTTATATGGGCTGGGTGACTTG
GAACATCCGTCCGAACACAAGCCAAAATGAGAAACATACGTTCACACCAAAAAAGCAAGCTGCGTTTGCGGCCGCTGTTT
CTCTTTTAAACCCGCATGCCATTCTGGACACGATCGGCGTCATCGGCACGAGCTCTCTTCAATATTCGGGGCTTGAGAAA
TGGCTCTTTATGGCCGCCTGCATCGCTGTGTCTTGGATTTGGTTCATCAGCCTTGCAATAGCGGGCCGGTTGTTTCAGAC
GATAGATACGAGCGGCAGGCTTATGCTGATCGTAAACAAATGCTCTGCAGCGGTAATGTGGGCAGCGGCAGGATATTTTG
GGGTGTCACTCTTTTGCAATTGA

Upstream 100 bases:

>100_bases
ACTTTCGGCTTGGCTGCCATTGCGACAAGGTCATGCCCCCCTTTTTTACACATTATACATTTGGCTGGTTCCTAACCCAA
CCAAATGGCTGGAGACTTTA

Downstream 100 bases:

>100_bases
AGGAGAAACAGATGAAAAAAACGAATTTCTTAGAAAAGGGGGCATGCTGATGACTGATGCAAAACAGTTTTATGACTATC
ATTCTTGGGCAAACAAGAAA

Product: aminoacid related metabolite efflux transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MTISLMIEKKCERSFFSMNAIIHGIVLAFGLILPLGVQNVFIFQQGALQKHIWRALPAVISASVCDTLLIVLAVAGVSVI
VQELPVFETVMMAGGFLFLLYMGWVTWNIRPNTSQNEKHTFTPKKQAAFAAAVSLLNPHAILDTIGVIGTSSLQYSGLEK
WLFMAACIAVSWIWFISLAIAGRLFQTIDTSGRLMLIVNKCSAAVMWAAAGYFGVSLFCN

Sequences:

>Translated_220_residues
MTISLMIEKKCERSFFSMNAIIHGIVLAFGLILPLGVQNVFIFQQGALQKHIWRALPAVISASVCDTLLIVLAVAGVSVI
VQELPVFETVMMAGGFLFLLYMGWVTWNIRPNTSQNEKHTFTPKKQAAFAAAVSLLNPHAILDTIGVIGTSSLQYSGLEK
WLFMAACIAVSWIWFISLAIAGRLFQTIDTSGRLMLIVNKCSAAVMWAAAGYFGVSLFCN
>Mature_219_residues
TISLMIEKKCERSFFSMNAIIHGIVLAFGLILPLGVQNVFIFQQGALQKHIWRALPAVISASVCDTLLIVLAVAGVSVIV
QELPVFETVMMAGGFLFLLYMGWVTWNIRPNTSQNEKHTFTPKKQAAFAAAVSLLNPHAILDTIGVIGTSSLQYSGLEKW
LFMAACIAVSWIWFISLAIAGRLFQTIDTSGRLMLIVNKCSAAVMWAAAGYFGVSLFCN

Specific function: Unknown

COG id: COG1279

COG function: function code R; Lysine efflux permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LysE/ArgO transporter (TC 2.A.75) family

Homologues:

Organism=Escherichia coli, GI1789290, Length=191, Percent_Identity=31.9371727748691, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YISU_BACSU (O06730)

Other databases:

- EMBL:   Y09476
- EMBL:   AL009126
- PIR:   D69838
- RefSeq:   NP_388967.1
- EnsemblBacteria:   EBBACT00000003679
- GeneID:   939338
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU10870
- NMPDR:   fig|224308.1.peg.1086
- GenoList:   BSU10870
- GeneTree:   EBGT00070000031912
- HOGENOM:   HBG683136
- OMA:   MRYGGAA
- PhylomeDB:   O06730
- ProtClustDB:   CLSK2752364
- BioCyc:   BSUB:BSU10870-MONOMER
- InterPro:   IPR004777
- InterPro:   IPR001123
- TIGRFAMs:   TIGR00948

Pfam domain/function: PF01810 LysE

EC number: NA

Molecular weight: Translated: 24066; Mature: 23935

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xe149200)-; HASH(0xe05d7ac)-; HASH(0xd047aa4)-; HASH(0xd5fce5c)-; HASH(0xdee6db0)-; HASH(0xdf6a4a0)-;

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTISLMIEKKCERSFFSMNAIIHGIVLAFGLILPLGVQNVFIFQQGALQKHIWRALPAVI
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEECCHHHHHHHHHHHHHH
SASVCDTLLIVLAVAGVSVIVQELPVFETVMMAGGFLFLLYMGWVTWNIRPNTSQNEKHT
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCC
FTPKKQAAFAAAVSLLNPHAILDTIGVIGTSSLQYSGLEKWLFMAACIAVSWIWFISLAI
CCCHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AGRLFQTIDTSGRLMLIVNKCSAAVMWAAAGYFGVSLFCN
HHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHEEEEEH
>Mature Secondary Structure 
TISLMIEKKCERSFFSMNAIIHGIVLAFGLILPLGVQNVFIFQQGALQKHIWRALPAVI
EEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEECCHHHHHHHHHHHHHH
SASVCDTLLIVLAVAGVSVIVQELPVFETVMMAGGFLFLLYMGWVTWNIRPNTSQNEKHT
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCC
FTPKKQAAFAAAVSLLNPHAILDTIGVIGTSSLQYSGLEKWLFMAACIAVSWIWFISLAI
CCCHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AGRLFQTIDTSGRLMLIVNKCSAAVMWAAAGYFGVSLFCN
HHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHEEEEEH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377