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The map label for this gene is yisB

Identifier: 16078130

GI number: 16078130

Start: 1148155

End: 1148457

Strand: Direct

Name: yisB

Synonym: BSU10660

Alternate gene names: 16078130

Gene position: 1148155-1148457 (Clockwise)

Preceding gene: 255767245

Following gene: 16078139

Centisome position: 27.24

GC content: 45.21

Gene sequence:

>303_bases
ATGGCAAAGCAGATTGCCGGAATATGTGAGCTGTGCGGCCGGAGCGATGTCCAGCTGACAGAGCATCATCTTACACCGAA
AGAGGAAGGCGGAACGTTTTTGCCGACAGCGATGCTCTGTATTCCTTGTCATAAACAGATTCATGCCTTATATACCAATC
AAGAGCTCGCTGTACGTTTAAATGGAATGGCTGAACTCAGAAGCGATCCAGAGCTTGCCCGGTTTGTCAAATGGATCAGA
AAACAGCCGCCTGAGAAGCTGATCAAAACTAAGAAATCAAATGAACGAAAAAAGAAAAAATGA

Upstream 100 bases:

>100_bases
CTGCGTGCACGGCTTCCGAAAAAGCTGATCGTCCATCCGGCTGAACCGAGCGGCCGCGGTACGCGGGTATCACTTGAGTT
GATGTAAGGGAGGAGCGGAT

Downstream 100 bases:

>100_bases
CTAAAAAGCAGCCCTCTCTTTGCAGAGCGGCTGCTTTTATCCGTTTCCAACCATATCCTGATCGTTGACATCGGGGTCTA
TATAATTTGTCGCGTTGACT

Product: nuclease component

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 100; Mature: 99

Protein sequence:

>100_residues
MAKQIAGICELCGRSDVQLTEHHLTPKEEGGTFLPTAMLCIPCHKQIHALYTNQELAVRLNGMAELRSDPELARFVKWIR
KQPPEKLIKTKKSNERKKKK

Sequences:

>Translated_100_residues
MAKQIAGICELCGRSDVQLTEHHLTPKEEGGTFLPTAMLCIPCHKQIHALYTNQELAVRLNGMAELRSDPELARFVKWIR
KQPPEKLIKTKKSNERKKKK
>Mature_99_residues
AKQIAGICELCGRSDVQLTEHHLTPKEEGGTFLPTAMLCIPCHKQIHALYTNQELAVRLNGMAELRSDPELARFVKWIRK
QPPEKLIKTKKSNERKKKK

Specific function: Unknown

COG id: COG1403

COG function: function code V; Restriction endonuclease

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YISB_BACSU (O06715)

Other databases:

- EMBL:   Y09476
- EMBL:   AL009126
- PIR:   B69836
- RefSeq:   NP_388947.1
- ProteinModelPortal:   O06715
- SMR:   O06715
- EnsemblBacteria:   EBBACT00000000415
- GeneID:   936345
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU10660
- NMPDR:   fig|224308.1.peg.1066
- GenoList:   BSU10660
- GeneTree:   EBGT00050000002358
- HOGENOM:   HBG536772
- OMA:   HARYSEA
- ProtClustDB:   CLSK887027
- BioCyc:   BSUB:BSU10660-MONOMER

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 11450; Mature: 11318

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQIAGICELCGRSDVQLTEHHLTPKEEGGTFLPTAMLCIPCHKQIHALYTNQELAVRL
CCHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEE
NGMAELRSDPELARFVKWIRKQPPEKLIKTKKSNERKKKK
CCHHHCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
AKQIAGICELCGRSDVQLTEHHLTPKEEGGTFLPTAMLCIPCHKQIHALYTNQELAVRL
CHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEE
NGMAELRSDPELARFVKWIRKQPPEKLIKTKKSNERKKKK
CCHHHCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377