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The map label for this gene is yisK

Identifier: 16078139

GI number: 16078139

Start: 1152244

End: 1153149

Strand: Direct

Name: yisK

Synonym: BSU10750

Alternate gene names: 16078139

Gene position: 1152244-1153149 (Clockwise)

Preceding gene: 16078130

Following gene: 16078140

Centisome position: 27.33

GC content: 46.47

Gene sequence:

>906_bases
ATGAAATTTGCGACAGGGGAACTTTACAACCGAATGTTTGTCGGCCTGATCATTGACGATGAGAAAATTATGGATTTGCA
GAAGGCTGAAAAAAAACTGTTTGAACTTGAGACGATTCCGGGATCGCTGATTGAATGTATCGCAGAAGGGGATAAATTCG
TTGCGCATGCAAGGCAGCTGGCCGAGTGGGCAAAGAAACCGAATGATGAGCTGGGATCATTTATGTATTCATTATCTGAG
GTGAAGCTCCATGCACCCATTCCGAAGCCATCAAAAAATATCATCTGCATCGGCAAAAACTACCGGGATCACGCGATTGA
AATGGGGAGCGAGGCTGATATTCCGGAGCATCCGATGGTATTTACAAAATCGCCGGTAACGGTTACGGGGCATGGTGATA
TCGTAAAGAGCCATGAAGAGGTTACCTCTCAGCTTGATTATGAGGGAGAACTTGCTGTCGTGATCGGAAAAAGCGGGACT
CGCATTTCAAAAGAAGATGCATATGACCATGTTTTCGGATATACGATTGTGAATGATATCACGGCGCGTGATCTGCAAAA
AAGGCATAAGCAGTTTTTTATCGGAAAAAGCTTGGATACGACATGTCCGATGGGGCCCGTGCTTGTACATAAATCATCTA
TTCAGGAGCCAGAGCGCCTCAAGGTTGAAACAAGAGTCAACGGCGAGCTGCGCCAATCCGGTTCGGCAAGCGATATGATC
TTTTCCATTCCGGAATTAATTGAAACCCTCTCAAAGGGGATGACGCTTGAAGCGGGCGACATTATTGCCACCGGTACGCC
GTCTGGTGTCGGCAAGGGGTTTACGCCTCCAAAATTCTTGCGGTCAGGTGACAAAATTGATATTACAATTGATCCGATCG
GAACGCTGTCAAACCAAATTGGCTGA

Upstream 100 bases:

>100_bases
CTGAAACGAAATGAAAACGGAATTGAGAAAAAGGGATTTCAGCATTCGACAGAGAATACATGAAGGAGCATTCTGATTTA
AAAGATGATGGAGGAATAAC

Downstream 100 bases:

>100_bases
ATAAAACTGGAGGGCGGACCCGGACCCGCCCGTTTTTTCTGATAATCATCTTTGTGACAGAGGACAAGTTCATGGTACTA
TAAGTGGGGTAATTTATCTG

Product: catabolic enzyme

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MKFATGELYNRMFVGLIIDDEKIMDLQKAEKKLFELETIPGSLIECIAEGDKFVAHARQLAEWAKKPNDELGSFMYSLSE
VKLHAPIPKPSKNIICIGKNYRDHAIEMGSEADIPEHPMVFTKSPVTVTGHGDIVKSHEEVTSQLDYEGELAVVIGKSGT
RISKEDAYDHVFGYTIVNDITARDLQKRHKQFFIGKSLDTTCPMGPVLVHKSSIQEPERLKVETRVNGELRQSGSASDMI
FSIPELIETLSKGMTLEAGDIIATGTPSGVGKGFTPPKFLRSGDKIDITIDPIGTLSNQIG

Sequences:

>Translated_301_residues
MKFATGELYNRMFVGLIIDDEKIMDLQKAEKKLFELETIPGSLIECIAEGDKFVAHARQLAEWAKKPNDELGSFMYSLSE
VKLHAPIPKPSKNIICIGKNYRDHAIEMGSEADIPEHPMVFTKSPVTVTGHGDIVKSHEEVTSQLDYEGELAVVIGKSGT
RISKEDAYDHVFGYTIVNDITARDLQKRHKQFFIGKSLDTTCPMGPVLVHKSSIQEPERLKVETRVNGELRQSGSASDMI
FSIPELIETLSKGMTLEAGDIIATGTPSGVGKGFTPPKFLRSGDKIDITIDPIGTLSNQIG
>Mature_301_residues
MKFATGELYNRMFVGLIIDDEKIMDLQKAEKKLFELETIPGSLIECIAEGDKFVAHARQLAEWAKKPNDELGSFMYSLSE
VKLHAPIPKPSKNIICIGKNYRDHAIEMGSEADIPEHPMVFTKSPVTVTGHGDIVKSHEEVTSQLDYEGELAVVIGKSGT
RISKEDAYDHVFGYTIVNDITARDLQKRHKQFFIGKSLDTTCPMGPVLVHKSSIQEPERLKVETRVNGELRQSGSASDMI
FSIPELIETLSKGMTLEAGDIIATGTPSGVGKGFTPPKFLRSGDKIDITIDPIGTLSNQIG

Specific function: Unknown

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family

Homologues:

Organism=Homo sapiens, GI156231349, Length=265, Percent_Identity=38.8679245283019, Blast_Score=191, Evalue=9e-49,
Organism=Homo sapiens, GI40786394, Length=265, Percent_Identity=38.1132075471698, Blast_Score=185, Evalue=4e-47,
Organism=Homo sapiens, GI66348062, Length=184, Percent_Identity=42.9347826086957, Blast_Score=138, Evalue=6e-33,
Organism=Homo sapiens, GI215422413, Length=184, Percent_Identity=42.9347826086957, Blast_Score=138, Evalue=6e-33,
Organism=Homo sapiens, GI13654274, Length=184, Percent_Identity=42.9347826086957, Blast_Score=138, Evalue=8e-33,
Organism=Escherichia coli, GI1787428, Length=206, Percent_Identity=33.9805825242718, Blast_Score=118, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17557057, Length=199, Percent_Identity=39.6984924623116, Blast_Score=140, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6324161, Length=234, Percent_Identity=29.4871794871795, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI28572127, Length=297, Percent_Identity=39.3939393939394, Blast_Score=183, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24663695, Length=221, Percent_Identity=38.9140271493213, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI28571789, Length=213, Percent_Identity=31.924882629108, Blast_Score=112, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YISK_BACSU (O06724)

Other databases:

- EMBL:   Y09476
- EMBL:   AL009126
- PIR:   C69837
- RefSeq:   NP_388956.1
- HSSP:   P37352
- ProteinModelPortal:   O06724
- SMR:   O06724
- EnsemblBacteria:   EBBACT00000000404
- GeneID:   936356
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU10750
- NMPDR:   fig|224308.1.peg.1075
- GenoList:   BSU10750
- GeneTree:   EBGT00050000001710
- HOGENOM:   HBG729228
- OMA:   VLEMGSK
- ProtClustDB:   CLSK887035
- BioCyc:   BSUB:BSU10750-MONOMER
- InterPro:   IPR002529
- InterPro:   IPR011234
- Gene3D:   G3DSA:3.90.850.10

Pfam domain/function: PF01557 FAA_hydrolase; SSF56529 Fumarylacetoacetase_C-rel

EC number: NA

Molecular weight: Translated: 33146; Mature: 33146

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFATGELYNRMFVGLIIDDEKIMDLQKAEKKLFELETIPGSLIECIAEGDKFVAHARQL
CCCCCHHHHHCEEEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHH
AEWAKKPNDELGSFMYSLSEVKLHAPIPKPSKNIICIGKNYRDHAIEMGSEADIPEHPMV
HHHHHCCCHHHHHHHHHHHHEEEECCCCCCCCCEEEEECCCCHHHHHCCCCCCCCCCCEE
FTKSPVTVTGHGDIVKSHEEVTSQLDYEGELAVVIGKSGTRISKEDAYDHVFGYTIVNDI
EECCCEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
TARDLQKRHKQFFIGKSLDTTCPMGPVLVHKSSIQEPERLKVETRVNGELRQSGSASDMI
HHHHHHHHHHHEECCCCCCCCCCCCCEEEECCCCCCCHHEEEEEECCCHHHCCCCCCHHH
FSIPELIETLSKGMTLEAGDIIATGTPSGVGKGFTPPKFLRSGDKIDITIDPIGTLSNQI
HHHHHHHHHHHCCCEEECCCEEEECCCCCCCCCCCCHHHHHCCCEEEEEECCCHHHHCCC
G
C
>Mature Secondary Structure
MKFATGELYNRMFVGLIIDDEKIMDLQKAEKKLFELETIPGSLIECIAEGDKFVAHARQL
CCCCCHHHHHCEEEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHH
AEWAKKPNDELGSFMYSLSEVKLHAPIPKPSKNIICIGKNYRDHAIEMGSEADIPEHPMV
HHHHHCCCHHHHHHHHHHHHEEEECCCCCCCCCEEEEECCCCHHHHHCCCCCCCCCCCEE
FTKSPVTVTGHGDIVKSHEEVTSQLDYEGELAVVIGKSGTRISKEDAYDHVFGYTIVNDI
EECCCEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
TARDLQKRHKQFFIGKSLDTTCPMGPVLVHKSSIQEPERLKVETRVNGELRQSGSASDMI
HHHHHHHHHHHEECCCCCCCCCCCCCEEEECCCCCCCHHEEEEEECCCHHHCCCCCCHHH
FSIPELIETLSKGMTLEAGDIIATGTPSGVGKGFTPPKFLRSGDKIDITIDPIGTLSNQI
HHHHHHHHHHHCCCEEECCCEEEECCCCCCCCCCCCHHHHHCCCEEEEEECCCHHHHCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377