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The map label for this gene is yhjG

Identifier: 16078114

GI number: 16078114

Start: 1122862

End: 1124361

Strand: Direct

Name: yhjG

Synonym: BSU10500

Alternate gene names: 16078114

Gene position: 1122862-1124361 (Clockwise)

Preceding gene: 16078113

Following gene: 16078115

Centisome position: 26.64

GC content: 53.33

Gene sequence:

>1500_bases
ATGATGATGAAATATGAAGCAGTGATTATAGGAGGAGGGCCGGTTGGATTTATGCTGGCTTCTGAGCTTGCAATAGCCGG
AGTGGGAACATGTGTGATTGAACGGCTTGAAAAACCTGTTCCGCATTCCAAGGCGTTGACGCTGCACCCCCGAACGCTGG
AGCTTCTTGAGATGAGAGGGATTTTAGAGCGGTTCGTATCCAAGGGAAGCAAGATACCTACCGGACATTTTTCAATGCTG
GATACCCGTCTGGATTTTTCAGGCTTGGATACGAGCTGTCCTTACACGCTTTTGCTCCCGCAGTCCAAAACAGAAAAGCT
TCTTGAAGATCATGCACGAAGCCTGGGAACAGAGGTGTTTCGGGGTGCCGAGGCGCTGGCGGTTACGCAAAACGGGGAAG
CGGTCCAAACAATTTTTAAGGATCGGGACGGCAGTGTACGGACGATCACAAGCAAGTTTGCCGTTGGGGCAGATGGCGCC
GGGAGTACCGTCCGGAAACAGGCGAAAATTGAGTTTCCGGGAACAGACAGCACTGTCACCGCTGCGCTTGGGGATGTGGT
GCTCCTTTCTCCGCCGCCGTCAGGTGTGCTGTCCCTTTGCACAAAAGAGGGCGGTGTTATGATTGTTCCGTTATCTCCAG
ACCGATACCGTGTCGTTGTGATCAGCCCTTATCGCACACAAACGCCGAAAGATGTGCCTGTGACTGAGGAAGAGCTGAAA
GCTGACCTCCTGCGAATTTGCGGAACTGACTTCGGTTTAACCGACCCTTCGTGGATGTCGCGGTTTGGGAACGCCGCGCG
CCAAGCGAAACGGTATCGGGACGGCAGAATCTTTCTTGCGGGTGATGCCGCGCATATTCATTTTCCGGCGGGCGGGCAAG
GACTGAATGTCGGCCTTCAGGATGCAATGAACCTCGGGTGGAAACTGGCGGCGGCCATCAAAGGCTCTGCGCCTTCTTGG
CTTTTGGACAGCTACCATGATGAGCGGCATCCGGCAGCGGAAGGGCTCCTCAGAAACACGGAGGCACAGACAAAGCTGAT
CGATTTCACTCAAGCAGGCCTCCATTTGCGAAGCATGATGTCGGAGCTGCTGGCCTTCCCTGATGTGAATCGATATGTAG
CGGGGCAAATTTCTGCGCTCGATGTCAGATATGAAGCTGATCGTACCATGCCGCCGAATCGTTTGAACGGGGCAAGGCTC
CCTGACATGAAGCTGATTCTTTCCGACGGCAATTCGGAACGGCTGTACAGCTTTCTCCAAAACGGGACATTCGTTTTGCT
GTCATTGAGGCAGGAAGCTGATGATCATATCGAAGTAAAAGGCTTACGTACGGTAACCGCCTCGCTTGCAGAACCGAATG
AAAAGTTGCGGAATGTGCATACCATATTGATTCGTCCCGATGGACATGTAGCCTGGGCGGTGGATGCATCAGCGCCCGAT
TGTTCCGAGGTGATTCAAAAAGGGATCAGCCGCTGGTTTTCCGTCACCAGCCGAGTATAA

Upstream 100 bases:

>100_bases
CAAAATTGTTACCTGTGTAACAAAATTTATATATTTTTTTGACTGGAGGGCCGTTTTTATTGGTTTTTGTTCGTTTTTCA
TGAAACAAAAGGAGTGGTGA

Downstream 100 bases:

>100_bases
TTCAATTCGATTGTTTCATCTGTAACAAAACGGTATGATAGAAGAAACAATCGAATGAAAAAGGATGGAAAACCCGATGA
ACACAGATCATACGAAACGC

Product: hypothetical protein

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MMMKYEAVIIGGGPVGFMLASELAIAGVGTCVIERLEKPVPHSKALTLHPRTLELLEMRGILERFVSKGSKIPTGHFSML
DTRLDFSGLDTSCPYTLLLPQSKTEKLLEDHARSLGTEVFRGAEALAVTQNGEAVQTIFKDRDGSVRTITSKFAVGADGA
GSTVRKQAKIEFPGTDSTVTAALGDVVLLSPPPSGVLSLCTKEGGVMIVPLSPDRYRVVVISPYRTQTPKDVPVTEEELK
ADLLRICGTDFGLTDPSWMSRFGNAARQAKRYRDGRIFLAGDAAHIHFPAGGQGLNVGLQDAMNLGWKLAAAIKGSAPSW
LLDSYHDERHPAAEGLLRNTEAQTKLIDFTQAGLHLRSMMSELLAFPDVNRYVAGQISALDVRYEADRTMPPNRLNGARL
PDMKLILSDGNSERLYSFLQNGTFVLLSLRQEADDHIEVKGLRTVTASLAEPNEKLRNVHTILIRPDGHVAWAVDASAPD
CSEVIQKGISRWFSVTSRV

Sequences:

>Translated_499_residues
MMMKYEAVIIGGGPVGFMLASELAIAGVGTCVIERLEKPVPHSKALTLHPRTLELLEMRGILERFVSKGSKIPTGHFSML
DTRLDFSGLDTSCPYTLLLPQSKTEKLLEDHARSLGTEVFRGAEALAVTQNGEAVQTIFKDRDGSVRTITSKFAVGADGA
GSTVRKQAKIEFPGTDSTVTAALGDVVLLSPPPSGVLSLCTKEGGVMIVPLSPDRYRVVVISPYRTQTPKDVPVTEEELK
ADLLRICGTDFGLTDPSWMSRFGNAARQAKRYRDGRIFLAGDAAHIHFPAGGQGLNVGLQDAMNLGWKLAAAIKGSAPSW
LLDSYHDERHPAAEGLLRNTEAQTKLIDFTQAGLHLRSMMSELLAFPDVNRYVAGQISALDVRYEADRTMPPNRLNGARL
PDMKLILSDGNSERLYSFLQNGTFVLLSLRQEADDHIEVKGLRTVTASLAEPNEKLRNVHTILIRPDGHVAWAVDASAPD
CSEVIQKGISRWFSVTSRV
>Mature_499_residues
MMMKYEAVIIGGGPVGFMLASELAIAGVGTCVIERLEKPVPHSKALTLHPRTLELLEMRGILERFVSKGSKIPTGHFSML
DTRLDFSGLDTSCPYTLLLPQSKTEKLLEDHARSLGTEVFRGAEALAVTQNGEAVQTIFKDRDGSVRTITSKFAVGADGA
GSTVRKQAKIEFPGTDSTVTAALGDVVLLSPPPSGVLSLCTKEGGVMIVPLSPDRYRVVVISPYRTQTPKDVPVTEEELK
ADLLRICGTDFGLTDPSWMSRFGNAARQAKRYRDGRIFLAGDAAHIHFPAGGQGLNVGLQDAMNLGWKLAAAIKGSAPSW
LLDSYHDERHPAAEGLLRNTEAQTKLIDFTQAGLHLRSMMSELLAFPDVNRYVAGQISALDVRYEADRTMPPNRLNGARL
PDMKLILSDGNSERLYSFLQNGTFVLLSLRQEADDHIEVKGLRTVTASLAEPNEKLRNVHTILIRPDGHVAWAVDASAPD
CSEVIQKGISRWFSVTSRV

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family

Homologues:

Organism=Escherichia coli, GI1786543, Length=385, Percent_Identity=26.7532467532467, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1789273, Length=321, Percent_Identity=25.2336448598131, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YHJG_BACSU (O07561)

Other databases:

- EMBL:   Y14081
- EMBL:   AL009126
- PIR:   F69833
- RefSeq:   NP_388931.1
- ProteinModelPortal:   O07561
- SMR:   O07561
- EnsemblBacteria:   EBBACT00000003246
- GeneID:   936325
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU10500
- NMPDR:   fig|224308.1.peg.1050
- GenoList:   BSU10500
- GeneTree:   EBGT00050000001114
- HOGENOM:   HBG672088
- OMA:   DAAHIHF
- ProtClustDB:   PRK08244
- BioCyc:   BSUB:BSU10500-MONOMER
- InterPro:   IPR002938
- InterPro:   IPR003042
- PRINTS:   PR00420

Pfam domain/function: PF01494 FAD_binding_3

EC number: 1.14.13.- [C]

Molecular weight: Translated: 54357; Mature: 54357

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMMKYEAVIIGGGPVGFMLASELAIAGVGTCVIERLEKPVPHSKALTLHPRTLELLEMRG
CCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHH
ILERFVSKGSKIPTGHFSMLDTRLDFSGLDTSCPYTLLLPQSKTEKLLEDHARSLGTEVF
HHHHHHHCCCCCCCCCHHHEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHH
RGAEALAVTQNGEAVQTIFKDRDGSVRTITSKFAVGADGAGSTVRKQAKIEFPGTDSTVT
CCCCEEEEECCCHHHHHHHHCCCCCEEEEEHHHCCCCCCCCCHHHHCEEEECCCCCCHHH
AALGDVVLLSPPPSGVLSLCTKEGGVMIVPLSPDRYRVVVISPYRTQTPKDVPVTEEELK
EECCCEEEECCCCCHHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCHHHHH
ADLLRICGTDFGLTDPSWMSRFGNAARQAKRYRDGRIFLAGDAAHIHFPAGGQGLNVGLQ
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEECCCCCCCCCCHH
DAMNLGWKLAAAIKGSAPSWLLDSYHDERHPAAEGLLRNTEAQTKLIDFTQAGLHLRSMM
HHHCCCEEEEEEECCCCCCHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SELLAFPDVNRYVAGQISALDVRYEADRTMPPNRLNGARLPDMKLILSDGNSERLYSFLQ
HHHHCCCCCCHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHC
NGTFVLLSLRQEADDHIEVKGLRTVTASLAEPNEKLRNVHTILIRPDGHVAWAVDASAPD
CCCEEEEEECCCCCCCEEEEEHHHHHHHHCCCHHHHCCEEEEEEECCCCEEEEEECCCCC
CSEVIQKGISRWFSVTSRV
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMMKYEAVIIGGGPVGFMLASELAIAGVGTCVIERLEKPVPHSKALTLHPRTLELLEMRG
CCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHH
ILERFVSKGSKIPTGHFSMLDTRLDFSGLDTSCPYTLLLPQSKTEKLLEDHARSLGTEVF
HHHHHHHCCCCCCCCCHHHEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHH
RGAEALAVTQNGEAVQTIFKDRDGSVRTITSKFAVGADGAGSTVRKQAKIEFPGTDSTVT
CCCCEEEEECCCHHHHHHHHCCCCCEEEEEHHHCCCCCCCCCHHHHCEEEECCCCCCHHH
AALGDVVLLSPPPSGVLSLCTKEGGVMIVPLSPDRYRVVVISPYRTQTPKDVPVTEEELK
EECCCEEEECCCCCHHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCHHHHH
ADLLRICGTDFGLTDPSWMSRFGNAARQAKRYRDGRIFLAGDAAHIHFPAGGQGLNVGLQ
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEECCCCCCCCCCHH
DAMNLGWKLAAAIKGSAPSWLLDSYHDERHPAAEGLLRNTEAQTKLIDFTQAGLHLRSMM
HHHCCCEEEEEEECCCCCCHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SELLAFPDVNRYVAGQISALDVRYEADRTMPPNRLNGARLPDMKLILSDGNSERLYSFLQ
HHHHCCCCCCHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHC
NGTFVLLSLRQEADDHIEVKGLRTVTASLAEPNEKLRNVHTILIRPDGHVAWAVDASAPD
CCCEEEEEECCCCCCCEEEEEHHHHHHHHCCCHHHHCCEEEEEEECCCCEEEEEECCCCC
CSEVIQKGISRWFSVTSRV
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377