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The map label for this gene is yhfS

Identifier: 16078099

GI number: 16078099

Start: 1109388

End: 1110482

Strand: Reverse

Name: yhfS

Synonym: BSU10350

Alternate gene names: 16078099

Gene position: 1110482-1109388 (Counterclockwise)

Preceding gene: 16078100

Following gene: 16078098

Centisome position: 26.34

GC content: 52.69

Gene sequence:

>1095_bases
ATGAATGCGGTTATTGTTGATGCAAAACGAACGATCTTTGGAAATCAAAACGGACTGCTGAAGCCCTTCCTGCCGGAGGA
TTTGGCGGCTCCCATCATCCGCTGTCTCAGCCGAAAGCTAGAGGATCAAGTTGACGAGGTCATTCTCGGAAACGCTACTG
GCAGAGGCGGCAACCTGGCCAGACTGTCAGCCCTTCAAGCCGGACTGCCTTTATCGGTTCCCGGAATGACAATTGACAGA
CAGTGCGGCTCCGGCCTTGAAGCTGTGCGCTATGCCTGCAGCCTTATTCAAGCGGGAGCCGGCACGATGTATATCGCGGG
CGGCTCAGAAAGCAGCAGCCAATCCCCTTTTTCAGAACGGGCTCGCTTTTCTCCAGATGCGATCGGCGATCCAGACATGG
GCATTGCGGCAGAATATACGGCTGCACGCTATTCCATCAGCAGAAGCATGCAGGATGAGTACGCGCTTCTCAGCCATCAA
CGCAGCAGGAACGCGCATGATGAAGGATTTTACCGTGAAGAAGTTGTTGCTCTCGGGGAATTGGAGACGGACGAAGCATT
TTTGAAAACGCGGCCAATAGAAGCGATTATTCCCCGTGCAAAGCCGGTTTTCGACACCAGCTCCGGAACAGTCACAGCAG
CCAACAGCAGTGGCATAGCAGACGGAGCAGCCGCTCTTTTGGTAATGGAAGAAGAAAAAGCAGCAGCCCTGGGACTTAAG
CCTGTGCTTCGGTTTATCGGCAGCGCTGTCAGCGGCATTCACCCCAACTTTCCGCCCGCGGCACCGGTTGTCGCGATTCG
TCAGCTCTTACATACACACGATGTAACACCTGATGATATCGATTTATTTGAAATCAATGAAGCCTTTGCCGTCAAAATTT
GTGTCTGCTCGCAAGAACTCGGCATTCCCTTTTCAAAAATCAATGTGCGCGGCGGCGCCTTAGCTCTTGGCCATCCGTAC
GGTGCATCAGGTGCAGCTCTGGTAACCAGATTGTTTTATGAAGCGAAAAGACGGCCAGACTGTCAATATGCTGTTGCAGC
CATCGGAAGCGGCGGCGGAATCGGACTGGCTTTATTATTTGAAGTTCTTGCATAG

Upstream 100 bases:

>100_bases
CGAAAAAATGGGTGTTTGCAGACAGCTTGCCGGAAACGAGCAGCGGAAAAATTGCCCGTTCCAGAGTGAAAAAATGGCTG
GAAGAGAGTGTACAGTATAA

Downstream 100 bases:

>100_bases
GCTTTTTCATAGGACACACGCTTTCGATTTAGTATAATAGCGATATGAATAAAGTCAGGAAGGGGATTTTATGACAGCCG
TTTGTTTAGTAAGACATGGA

Product: acetyl-CoA acetyltransferase

Products: CoA; acetoacetyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MNAVIVDAKRTIFGNQNGLLKPFLPEDLAAPIIRCLSRKLEDQVDEVILGNATGRGGNLARLSALQAGLPLSVPGMTIDR
QCGSGLEAVRYACSLIQAGAGTMYIAGGSESSSQSPFSERARFSPDAIGDPDMGIAAEYTAARYSISRSMQDEYALLSHQ
RSRNAHDEGFYREEVVALGELETDEAFLKTRPIEAIIPRAKPVFDTSSGTVTAANSSGIADGAAALLVMEEEKAAALGLK
PVLRFIGSAVSGIHPNFPPAAPVVAIRQLLHTHDVTPDDIDLFEINEAFAVKICVCSQELGIPFSKINVRGGALALGHPY
GASGAALVTRLFYEAKRRPDCQYAVAAIGSGGGIGLALLFEVLA

Sequences:

>Translated_364_residues
MNAVIVDAKRTIFGNQNGLLKPFLPEDLAAPIIRCLSRKLEDQVDEVILGNATGRGGNLARLSALQAGLPLSVPGMTIDR
QCGSGLEAVRYACSLIQAGAGTMYIAGGSESSSQSPFSERARFSPDAIGDPDMGIAAEYTAARYSISRSMQDEYALLSHQ
RSRNAHDEGFYREEVVALGELETDEAFLKTRPIEAIIPRAKPVFDTSSGTVTAANSSGIADGAAALLVMEEEKAAALGLK
PVLRFIGSAVSGIHPNFPPAAPVVAIRQLLHTHDVTPDDIDLFEINEAFAVKICVCSQELGIPFSKINVRGGALALGHPY
GASGAALVTRLFYEAKRRPDCQYAVAAIGSGGGIGLALLFEVLA
>Mature_364_residues
MNAVIVDAKRTIFGNQNGLLKPFLPEDLAAPIIRCLSRKLEDQVDEVILGNATGRGGNLARLSALQAGLPLSVPGMTIDR
QCGSGLEAVRYACSLIQAGAGTMYIAGGSESSSQSPFSERARFSPDAIGDPDMGIAAEYTAARYSISRSMQDEYALLSHQ
RSRNAHDEGFYREEVVALGELETDEAFLKTRPIEAIIPRAKPVFDTSSGTVTAANSSGIADGAAALLVMEEEKAAALGLK
PVLRFIGSAVSGIHPNFPPAAPVVAIRQLLHTHDVTPDDIDLFEINEAFAVKICVCSQELGIPFSKINVRGGALALGHPY
GASGAALVTRLFYEAKRRPDCQYAVAAIGSGGGIGLALLFEVLA

Specific function: May be involved in fatty acid metabolism

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family

Homologues:

Organism=Homo sapiens, GI148539872, Length=397, Percent_Identity=36.7758186397985, Blast_Score=218, Evalue=9e-57,
Organism=Homo sapiens, GI167614485, Length=392, Percent_Identity=35.2040816326531, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI4557237, Length=390, Percent_Identity=33.5897435897436, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI4501853, Length=389, Percent_Identity=34.1902313624679, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI4504327, Length=426, Percent_Identity=31.924882629108, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI194353979, Length=366, Percent_Identity=29.2349726775956, Blast_Score=125, Evalue=5e-29,
Organism=Escherichia coli, GI1788554, Length=394, Percent_Identity=38.8324873096447, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI48994986, Length=382, Percent_Identity=37.17277486911, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1787663, Length=399, Percent_Identity=37.593984962406, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI87082165, Length=391, Percent_Identity=37.5959079283887, Blast_Score=197, Evalue=9e-52,
Organism=Escherichia coli, GI1788683, Length=415, Percent_Identity=28.1927710843373, Blast_Score=122, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI133906874, Length=389, Percent_Identity=38.3033419023136, Blast_Score=221, Evalue=4e-58,
Organism=Caenorhabditis elegans, GI17535921, Length=349, Percent_Identity=35.243553008596, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI25147385, Length=391, Percent_Identity=33.5038363171356, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17551802, Length=421, Percent_Identity=29.6912114014252, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17535917, Length=377, Percent_Identity=29.7082228116711, Blast_Score=114, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6322031, Length=393, Percent_Identity=33.0788804071247, Blast_Score=166, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6325229, Length=398, Percent_Identity=31.4070351758794, Blast_Score=150, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24655093, Length=389, Percent_Identity=38.0462724935733, Blast_Score=229, Evalue=3e-60,
Organism=Drosophila melanogaster, GI17648125, Length=396, Percent_Identity=34.0909090909091, Blast_Score=191, Evalue=7e-49,
Organism=Drosophila melanogaster, GI24640423, Length=390, Percent_Identity=31.7948717948718, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI17137578, Length=426, Percent_Identity=31.4553990610329, Blast_Score=145, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YHFS_BACSU (O07618)

Other databases:

- EMBL:   Y14084
- EMBL:   AL009126
- PIR:   H69831
- RefSeq:   NP_388916.1
- HSSP:   P27796
- ProteinModelPortal:   O07618
- SMR:   O07618
- EnsemblBacteria:   EBBACT00000002846
- GeneID:   939311
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU10350
- NMPDR:   fig|224308.1.peg.1035
- GenoList:   BSU10350
- GeneTree:   EBGT00070000032174
- HOGENOM:   HBG370930
- OMA:   ANSCKEA
- ProtClustDB:   PRK06690
- BioCyc:   BSUB:BSU10350-MONOMER
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616
- Gene3D:   G3DSA:3.40.47.10
- PANTHER:   PTHR18919
- PIRSF:   PIRSF000429
- TIGRFAMs:   TIGR01930

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N; SSF53901 Thiolase-like

EC number: 2.3.1.9

Molecular weight: Translated: 38399; Mature: 38399

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00098 THIOLASE_1; PS00737 THIOLASE_2; PS00099 THIOLASE_3

Important sites: ACT_SITE 82-82 ACT_SITE 318-318

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAVIVDAKRTIFGNQNGLLKPFLPEDLAAPIIRCLSRKLEDQVDEVILGNATGRGGNLA
CCEEEEECCHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHH
RLSALQAGLPLSVPGMTIDRQCGSGLEAVRYACSLIQAGAGTMYIAGGSESSSQSPFSER
HHHHHHCCCCCCCCCCEECHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHH
ARFSPDAIGDPDMGIAAEYTAARYSISRSMQDEYALLSHQRSRNAHDEGFYREEVVALGE
HCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
LETDEAFLKTRPIEAIIPRAKPVFDTSSGTVTAANSSGIADGAAALLVMEEEKAAALGLK
CCCCHHHHHCCCHHHHCCCCCCCEECCCCEEEECCCCCCCCCCEEEEEEECCHHHHCCHH
PVLRFIGSAVSGIHPNFPPAAPVVAIRQLLHTHDVTPDDIDLFEINEAFAVKICVCSQEL
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCEEEEEEEEECHHH
GIPFSKINVRGGALALGHPYGASGAALVTRLFYEAKRRPDCQYAVAAIGSGGGIGLALLF
CCCHHHEEECCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHH
EVLA
HHHC
>Mature Secondary Structure
MNAVIVDAKRTIFGNQNGLLKPFLPEDLAAPIIRCLSRKLEDQVDEVILGNATGRGGNLA
CCEEEEECCHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHH
RLSALQAGLPLSVPGMTIDRQCGSGLEAVRYACSLIQAGAGTMYIAGGSESSSQSPFSER
HHHHHHCCCCCCCCCCEECHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHH
ARFSPDAIGDPDMGIAAEYTAARYSISRSMQDEYALLSHQRSRNAHDEGFYREEVVALGE
HCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
LETDEAFLKTRPIEAIIPRAKPVFDTSSGTVTAANSSGIADGAAALLVMEEEKAAALGLK
CCCCHHHHHCCCHHHHCCCCCCCEECCCCEEEECCCCCCCCCCEEEEEEECCHHHHCCHH
PVLRFIGSAVSGIHPNFPPAAPVVAIRQLLHTHDVTPDDIDLFEINEAFAVKICVCSQEL
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCEEEEEEEEECHHH
GIPFSKINVRGGALALGHPYGASGAALVTRLFYEAKRRPDCQYAVAAIGSGGGIGLALLF
CCCHHHEEECCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHH
EVLA
HHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acetyl-CoA

Specific reaction: 2 acetyl-CoA = CoA + acetoacetyl-CoA

General reaction: Acyl group transfer; Condensation; Thiolytic cleavage [C]

Inhibitor: Acetoacetyl-CoA; Borohydride; Iodoacetamide; N-Ethyl maleimide; p-Chloromercuribenzoate; Sulfhydryl reagents [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377