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The map label for this gene is yhfT

Identifier: 16078100

GI number: 16078100

Start: 1110479

End: 1111918

Strand: Reverse

Name: yhfT

Synonym: BSU10360

Alternate gene names: 16078100

Gene position: 1111918-1110479 (Counterclockwise)

Preceding gene: 16078101

Following gene: 16078099

Centisome position: 26.38

GC content: 49.1

Gene sequence:

>1440_bases
ATGACAATTACTCATACCTATTCATCTACTGCCGAAACATCGCCCGGCCGTGTAGCGATCCAGACTGAATCGGAGCAAAT
CACGTACCATGATTGGGATCGGCTTGTCTCTCAAACCGCAAATTGGCTGCGGTCACAGCCGAGCATGCCGAATCGTGTGG
CGATCCTGCTCCCAAATAGTCTCGCGTTTTTACAGCTGTTTGCCGGAGCCGCAGCGGCTGGATGTACGGCCATTCCCATC
GACACACGCTGGAGCCCGGCTGAATGTAAGGAGCGGCTGTCCATAAGCAATGCGGATCTTGTGGTTACTTTAGCCTTTTT
CAAAAACAAACTGACAGATAGCCAGACACCTGTTGTATTGCTGGATAACTGTATGGCAGATATTTCTGAGGCAGCCGCTG
ATCCCTTGCCTACCATTGATCCGGAGCACCCTTTTTATATGGGATTTACGTCGGGCTCGACAGGAAAACCGAAGGCCTTT
ACGCGATCTCACCGCTCATGGATGGAGAGCTTTACCTGTACAGAAACAGATTTTTCGATTTCATCAGATGATAAGGTTCT
GATTCCCGGAGCGTTAATGTCCTCTCACTTCCTATATGGGGCTGTCAGCACTTTGTTTCTCGGAGGAACCGTTTGTTTGC
TGAAAAAGTTTTCTCCTGCCAAAGCGAAGGAATGGCTGTGCCGTGAATCCATCAGTGTTCTCTATACCGTACCGACGATG
ACAGACGCCCTCGCAAGGATTGAGGGTTTTCCCGACAGTCCCGTCAAAATCATTTCATCCGGCGCAGACTGGCCGGCAGA
ATCCAAGAAGAAGCTTGCCGCTGCATGGCCTCATCTCAAGCTGTACGATTTTTACGGCACATCAGAGCTTAGTTTTGTGA
CGTTTTCTTCACCGGAAGACAGCAAACGGAAGCCGCATTCAGCGGGCCGCCCTTTTCATAATGTCCGGATCGAAATCCGC
AACGCTGGAGGAGAACGCTGCCAGCCAGGAGAAATCGGAAAAATATTTGTCAAAAGCCCGATGAGGTTTTCCGGCTATGT
GAACGGCAGCACACCAGATGAATGGATGACAGTAGATGATATGGGCTACGTTGATGAAGAGGGCTTTCTATACATATCAG
GAAGAGAAAACGGGATGATCGTGTACGGAGGATTAAATATTTTCCCAGAAGAAATTGAACGTGTGCTTCTCGCCTGCCCA
GAGGTTGAAAGCGCGGCTGTCGTTGGCATTCCCGACGAGTATTGGGGAGAAATCGCTGTAGCTGTCATTCTTGGAAACGC
TAATGCCAGAACACTGAAAGCCTGGTGTAAACAGAAATTAGCCTCCTATAAAATTCCGAAAAAATGGGTGTTTGCAGACA
GCTTGCCGGAAACGAGCAGCGGAAAAATTGCCCGTTCCAGAGTGAAAAAATGGCTGGAAGAGAGTGTACAGTATAAATGA

Upstream 100 bases:

>100_bases
TGATTTGATAAAAGCGGCTGTATCTGCATTTCTGGCGATAAAAATCACTCAAGCCTTGTCTCTTTCTGATACGATGTTTA
CAAAAGGAGGATGACAATAC

Downstream 100 bases:

>100_bases
ATGCGGTTATTGTTGATGCAAAACGAACGATCTTTGGAAATCAAAACGGACTGCTGAAGCCCTTCCTGCCGGAGGATTTG
GCGGCTCCCATCATCCGCTG

Product: acyl-CoA synthetase

Products: AMP; diphosphate; acyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MTITHTYSSTAETSPGRVAIQTESEQITYHDWDRLVSQTANWLRSQPSMPNRVAILLPNSLAFLQLFAGAAAAGCTAIPI
DTRWSPAECKERLSISNADLVVTLAFFKNKLTDSQTPVVLLDNCMADISEAAADPLPTIDPEHPFYMGFTSGSTGKPKAF
TRSHRSWMESFTCTETDFSISSDDKVLIPGALMSSHFLYGAVSTLFLGGTVCLLKKFSPAKAKEWLCRESISVLYTVPTM
TDALARIEGFPDSPVKIISSGADWPAESKKKLAAAWPHLKLYDFYGTSELSFVTFSSPEDSKRKPHSAGRPFHNVRIEIR
NAGGERCQPGEIGKIFVKSPMRFSGYVNGSTPDEWMTVDDMGYVDEEGFLYISGRENGMIVYGGLNIFPEEIERVLLACP
EVESAAVVGIPDEYWGEIAVAVILGNANARTLKAWCKQKLASYKIPKKWVFADSLPETSSGKIARSRVKKWLEESVQYK

Sequences:

>Translated_479_residues
MTITHTYSSTAETSPGRVAIQTESEQITYHDWDRLVSQTANWLRSQPSMPNRVAILLPNSLAFLQLFAGAAAAGCTAIPI
DTRWSPAECKERLSISNADLVVTLAFFKNKLTDSQTPVVLLDNCMADISEAAADPLPTIDPEHPFYMGFTSGSTGKPKAF
TRSHRSWMESFTCTETDFSISSDDKVLIPGALMSSHFLYGAVSTLFLGGTVCLLKKFSPAKAKEWLCRESISVLYTVPTM
TDALARIEGFPDSPVKIISSGADWPAESKKKLAAAWPHLKLYDFYGTSELSFVTFSSPEDSKRKPHSAGRPFHNVRIEIR
NAGGERCQPGEIGKIFVKSPMRFSGYVNGSTPDEWMTVDDMGYVDEEGFLYISGRENGMIVYGGLNIFPEEIERVLLACP
EVESAAVVGIPDEYWGEIAVAVILGNANARTLKAWCKQKLASYKIPKKWVFADSLPETSSGKIARSRVKKWLEESVQYK
>Mature_478_residues
TITHTYSSTAETSPGRVAIQTESEQITYHDWDRLVSQTANWLRSQPSMPNRVAILLPNSLAFLQLFAGAAAAGCTAIPID
TRWSPAECKERLSISNADLVVTLAFFKNKLTDSQTPVVLLDNCMADISEAAADPLPTIDPEHPFYMGFTSGSTGKPKAFT
RSHRSWMESFTCTETDFSISSDDKVLIPGALMSSHFLYGAVSTLFLGGTVCLLKKFSPAKAKEWLCRESISVLYTVPTMT
DALARIEGFPDSPVKIISSGADWPAESKKKLAAAWPHLKLYDFYGTSELSFVTFSSPEDSKRKPHSAGRPFHNVRIEIRN
AGGERCQPGEIGKIFVKSPMRFSGYVNGSTPDEWMTVDDMGYVDEEGFLYISGRENGMIVYGGLNIFPEEIERVLLACPE
VESAAVVGIPDEYWGEIAVAVILGNANARTLKAWCKQKLASYKIPKKWVFADSLPETSSGKIARSRVKKWLEESVQYK

Specific function: May be involved in fatty acid metabolism

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family

Homologues:

Organism=Homo sapiens, GI156151445, Length=551, Percent_Identity=25.4083484573503, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI187761345, Length=474, Percent_Identity=24.0506329113924, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI187761343, Length=474, Percent_Identity=24.0506329113924, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI42544132, Length=508, Percent_Identity=23.0314960629921, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI38505220, Length=470, Percent_Identity=24.6808510638298, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI122937307, Length=361, Percent_Identity=25.207756232687, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI157311624, Length=472, Percent_Identity=25.4237288135593, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI157311622, Length=472, Percent_Identity=25.4237288135593, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI115511026, Length=466, Percent_Identity=24.2489270386266, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI58082049, Length=477, Percent_Identity=24.1090146750524, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1788107, Length=480, Percent_Identity=25, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI145693145, Length=513, Percent_Identity=25.3411306042885, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI1786810, Length=349, Percent_Identity=27.7936962750716, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI221142682, Length=507, Percent_Identity=25.2465483234714, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790505, Length=525, Percent_Identity=24.5714285714286, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789201, Length=478, Percent_Identity=23.6401673640167, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788595, Length=346, Percent_Identity=23.9884393063584, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1786801, Length=443, Percent_Identity=22.5733634311512, Blast_Score=69, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI32563687, Length=472, Percent_Identity=22.4576271186441, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI71994690, Length=358, Percent_Identity=26.536312849162, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71994703, Length=358, Percent_Identity=26.536312849162, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71994694, Length=358, Percent_Identity=26.536312849162, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17531443, Length=524, Percent_Identity=24.236641221374, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17558820, Length=482, Percent_Identity=25.3112033195021, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17559526, Length=497, Percent_Identity=26.1569416498994, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17538037, Length=357, Percent_Identity=27.4509803921569, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI71996755, Length=490, Percent_Identity=23.6734693877551, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17557194, Length=362, Percent_Identity=26.5193370165746, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71985884, Length=379, Percent_Identity=27.7044854881267, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17560308, Length=363, Percent_Identity=27.8236914600551, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI25141359, Length=462, Percent_Identity=26.4069264069264, Blast_Score=99, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17560140, Length=365, Percent_Identity=25.2054794520548, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71982997, Length=395, Percent_Identity=25.5696202531646, Blast_Score=75, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI71983001, Length=395, Percent_Identity=25.5696202531646, Blast_Score=75, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=347, Percent_Identity=27.0893371757925, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6323182, Length=538, Percent_Identity=22.4907063197026, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319264, Length=390, Percent_Identity=23.8461538461538, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24581924, Length=477, Percent_Identity=25.5765199161426, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI18859661, Length=483, Percent_Identity=26.5010351966874, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24656500, Length=352, Percent_Identity=25.8522727272727, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI20130357, Length=346, Percent_Identity=24.5664739884393, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19922652, Length=374, Percent_Identity=23.2620320855615, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI21356947, Length=214, Percent_Identity=29.9065420560748, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI281365686, Length=402, Percent_Identity=23.3830845771144, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24648255, Length=339, Percent_Identity=26.8436578171091, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24648257, Length=354, Percent_Identity=26.271186440678, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24648253, Length=346, Percent_Identity=26.5895953757225, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648260, Length=366, Percent_Identity=24.0437158469945, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24653035, Length=198, Percent_Identity=29.2929292929293, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21355181, Length=466, Percent_Identity=22.5321888412017, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21358303, Length=180, Percent_Identity=28.8888888888889, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI21356441, Length=348, Percent_Identity=24.1379310344828, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24667959, Length=381, Percent_Identity=27.0341207349081, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24667955, Length=381, Percent_Identity=27.0341207349081, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI62472339, Length=381, Percent_Identity=27.0341207349081, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI161076582, Length=201, Percent_Identity=27.363184079602, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21355225, Length=413, Percent_Identity=21.7917675544794, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YHFT_BACSU (O07619)

Other databases:

- EMBL:   Y14084
- EMBL:   AL009126
- PIR:   A69832
- RefSeq:   NP_388917.1
- HSSP:   P08659
- ProteinModelPortal:   O07619
- SMR:   O07619
- EnsemblBacteria:   EBBACT00000000532
- GeneID:   936315
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU10360
- NMPDR:   fig|224308.1.peg.1036
- GenoList:   BSU10360
- GeneTree:   EBGT00070000031792
- HOGENOM:   HBG337818
- ProtClustDB:   PRK07638
- BioCyc:   BSUB:BSU10360-MONOMER
- InterPro:   IPR020845
- InterPro:   IPR000873

Pfam domain/function: PF00501 AMP-binding

EC number: 6.2.1.3

Molecular weight: Translated: 52882; Mature: 52750

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00455 AMP_BINDING

Important sites: BINDING 360-360 BINDING 375-375 BINDING 462-462

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTITHTYSSTAETSPGRVAIQTESEQITYHDWDRLVSQTANWLRSQPSMPNRVAILLPNS
CEEEEECCCCCCCCCCEEEEEECCCEEEEHHHHHHHHHHHHHHHCCCCCCCEEEEEECCH
LAFLQLFAGAAAAGCTAIPIDTRWSPAECKERLSISNADLVVTLAFFKNKLTDSQTPVVL
HHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHCCCCCCEEEEEEHHHHHCCCCCCCEEE
LDNCMADISEAAADPLPTIDPEHPFYMGFTSGSTGKPKAFTRSHRSWMESFTCTETDFSI
EHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
SSDDKVLIPGALMSSHFLYGAVSTLFLGGTVCLLKKFSPAKAKEWLCRESISVLYTVPTM
CCCCCEEECCHHHHHHHHHHHHHHHHHCCCEEEHCCCCCHHHHHHHHHCCCEEEEECCHH
TDALARIEGFPDSPVKIISSGADWPAESKKKLAAAWPHLKLYDFYGTSELSFVTFSSPED
HHHHHHHCCCCCCCHHEEECCCCCCCHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCCC
SKRKPHSAGRPFHNVRIEIRNAGGERCQPGEIGKIFVKSPMRFSGYVNGSTPDEWMTVDD
CCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEECCCEEEEEECCCCCCCEEEECC
MGYVDEEGFLYISGRENGMIVYGGLNIFPEEIERVLLACPEVESAAVVGIPDEYWGEIAV
CCCCCCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCEEEECCHHHHCCEEE
AVILGNANARTLKAWCKQKLASYKIPKKWVFADSLPETSSGKIARSRVKKWLEESVQYK
EEEECCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TITHTYSSTAETSPGRVAIQTESEQITYHDWDRLVSQTANWLRSQPSMPNRVAILLPNS
EEEEECCCCCCCCCCEEEEEECCCEEEEHHHHHHHHHHHHHHHCCCCCCCEEEEEECCH
LAFLQLFAGAAAAGCTAIPIDTRWSPAECKERLSISNADLVVTLAFFKNKLTDSQTPVVL
HHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHCCCCCCEEEEEEHHHHHCCCCCCCEEE
LDNCMADISEAAADPLPTIDPEHPFYMGFTSGSTGKPKAFTRSHRSWMESFTCTETDFSI
EHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
SSDDKVLIPGALMSSHFLYGAVSTLFLGGTVCLLKKFSPAKAKEWLCRESISVLYTVPTM
CCCCCEEECCHHHHHHHHHHHHHHHHHCCCEEEHCCCCCHHHHHHHHHCCCEEEEECCHH
TDALARIEGFPDSPVKIISSGADWPAESKKKLAAAWPHLKLYDFYGTSELSFVTFSSPED
HHHHHHHCCCCCCCHHEEECCCCCCCHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCCC
SKRKPHSAGRPFHNVRIEIRNAGGERCQPGEIGKIFVKSPMRFSGYVNGSTPDEWMTVDD
CCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEECCCEEEEEECCCCCCCEEEECC
MGYVDEEGFLYISGRENGMIVYGGLNIFPEEIERVLLACPEVESAAVVGIPDEYWGEIAV
CCCCCCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCEEEECCHHHHCCEEE
AVILGNANARTLKAWCKQKLASYKIPKKWVFADSLPETSSGKIARSRVKKWLEESVQYK
EEEECCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; long-chain carboxylic acid; CoA

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377