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The map label for this gene is yhfJ

Identifier: 16078089

GI number: 16078089

Start: 1099159

End: 1100154

Strand: Direct

Name: yhfJ

Synonym: BSU10250

Alternate gene names: 16078089

Gene position: 1099159-1100154 (Clockwise)

Preceding gene: 16078088

Following gene: 16078090

Centisome position: 26.07

GC content: 42.17

Gene sequence:

>996_bases
ATGTTATTTATAGACAATCAAAATATCAATGATCCGCGGATCAATCTTGCTATCGAGGAGTACTGTGTAAAGCATTTAGA
CCCTGAACAGCAATATTTGCTCTTTTATGTGAATCAACCGTCTATTATCATTGGGAAAAACCAAAATACAATAGAAGAAA
TCAATACAAAATATGTTGAGGAAAACGGGATTATCGTCGTCCGCCGTTTATCAGGCGGGGGCGCTGTGTACCATGATCTG
GGGAACTTGAACTTCAGCTTTATTACCAAGGATGACGGGGACAGCTTTCATAACTTTAAAAAATTCACTGAGCCAGTGAT
TCAGGCGTTACATCAGCTTGGGGTTGAAGCCGAATTAAGCGGCCGGAACGACATTGTAGTAGATGGCCGGAAAATATCCG
GAAACGCTCAGTTTGCAACGAAAGGCCGTATTTTCAGCCACGGCACCCTCATGTTTGATTCAGCCATTGATCATGTTGTA
TCAGCATTAAAGGTGAAAAAGGATAAAATTGAATCAAAAGGCATCAAATCGATCAGAAGCCGAGTGGCAAACATCAGTGA
GTTTCTCGATGATAAAATGACCACCGAAGAATTCCGCAGCCATTTGCTTCGCCATATTTTTAACACAAACGACGTTGGAA
ACGTGCCGGAGTATAAGCTGACGGAAAAAGATTGGGAGACCATTCATCAAATTTCGAAAGAGCGCTATCAGAATTGGGAT
TGGAACTACGGCCGCTCACCAAAATTTAACCTTAATCATTCGAAGCGTTATCCGGTTGGATCGATCGATTTGCACCTGGA
AGTCAAGAAAGGCAAAATCGAGGACTGCAAAATCTTCGGAGATTTCTTCGGTGTCGGTGATGTATCTGAAATTGAAAACC
TGCTGGTCGGAAAACAATACGAACGCAGCGTCATCGCTGATGTGCTCGAAGGTGTGAATCTCAAGCATTACTTTGGGAAC
ATTACGAAAGAGGATTTCCTTGATCTGATTTACTAA

Upstream 100 bases:

>100_bases
CGTGCATGACAACCTTCGCAAAGAAGCGAAAACCGTTTTTAACGGAGAAGTGAATATTGCGAAATCAGGTTTTGTGTGGG
AAGGATAAAGGAGGAGCACC

Downstream 100 bases:

>100_bases
GGCAAAACACATCGTTTGAAAGAGCGGTTGTGTTTTTGAAATAATGGAGGCAGGAGGGATTCACATGAAAGTGTTTTTAA
TCGGAGCGAACGGACAAATC

Product: lipoate-protein ligase

Products: NA

Alternate protein names: Lipoate--protein ligase

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MLFIDNQNINDPRINLAIEEYCVKHLDPEQQYLLFYVNQPSIIIGKNQNTIEEINTKYVEENGIIVVRRLSGGGAVYHDL
GNLNFSFITKDDGDSFHNFKKFTEPVIQALHQLGVEAELSGRNDIVVDGRKISGNAQFATKGRIFSHGTLMFDSAIDHVV
SALKVKKDKIESKGIKSIRSRVANISEFLDDKMTTEEFRSHLLRHIFNTNDVGNVPEYKLTEKDWETIHQISKERYQNWD
WNYGRSPKFNLNHSKRYPVGSIDLHLEVKKGKIEDCKIFGDFFGVGDVSEIENLLVGKQYERSVIADVLEGVNLKHYFGN
ITKEDFLDLIY

Sequences:

>Translated_331_residues
MLFIDNQNINDPRINLAIEEYCVKHLDPEQQYLLFYVNQPSIIIGKNQNTIEEINTKYVEENGIIVVRRLSGGGAVYHDL
GNLNFSFITKDDGDSFHNFKKFTEPVIQALHQLGVEAELSGRNDIVVDGRKISGNAQFATKGRIFSHGTLMFDSAIDHVV
SALKVKKDKIESKGIKSIRSRVANISEFLDDKMTTEEFRSHLLRHIFNTNDVGNVPEYKLTEKDWETIHQISKERYQNWD
WNYGRSPKFNLNHSKRYPVGSIDLHLEVKKGKIEDCKIFGDFFGVGDVSEIENLLVGKQYERSVIADVLEGVNLKHYFGN
ITKEDFLDLIY
>Mature_331_residues
MLFIDNQNINDPRINLAIEEYCVKHLDPEQQYLLFYVNQPSIIIGKNQNTIEEINTKYVEENGIIVVRRLSGGGAVYHDL
GNLNFSFITKDDGDSFHNFKKFTEPVIQALHQLGVEAELSGRNDIVVDGRKISGNAQFATKGRIFSHGTLMFDSAIDHVV
SALKVKKDKIESKGIKSIRSRVANISEFLDDKMTTEEFRSHLLRHIFNTNDVGNVPEYKLTEKDWETIHQISKERYQNWD
WNYGRSPKFNLNHSKRYPVGSIDLHLEVKKGKIEDCKIFGDFFGVGDVSEIENLLVGKQYERSVIADVLEGVNLKHYFGN
ITKEDFLDLIY

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family

Homologues:

Organism=Homo sapiens, GI7706252, Length=289, Percent_Identity=34.2560553633218, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI21729884, Length=289, Percent_Identity=34.2560553633218, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI21729882, Length=289, Percent_Identity=34.2560553633218, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI21729880, Length=289, Percent_Identity=34.2560553633218, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI21729878, Length=289, Percent_Identity=34.2560553633218, Blast_Score=132, Evalue=7e-31,
Organism=Escherichia coli, GI1790846, Length=317, Percent_Identity=34.0694006309148, Blast_Score=186, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI71981909, Length=271, Percent_Identity=31.7343173431734, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6322415, Length=279, Percent_Identity=30.4659498207885, Blast_Score=115, Evalue=9e-27,
Organism=Drosophila melanogaster, GI28573464, Length=252, Percent_Identity=31.3492063492063, Blast_Score=115, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LPLA_BACSU (O07608)

Other databases:

- EMBL:   Y14083
- EMBL:   AL009126
- PIR:   G69830
- RefSeq:   NP_388906.1
- HSSP:   P32099
- ProteinModelPortal:   O07608
- SMR:   O07608
- EnsemblBacteria:   EBBACT00000001700
- GeneID:   939310
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU10250
- NMPDR:   fig|224308.1.peg.1025
- GenoList:   BSU10250
- GeneTree:   EBGT00050000002150
- HOGENOM:   HBG715377
- OMA:   KGRNDLL
- PhylomeDB:   O07608
- ProtClustDB:   CLSK2391267
- BioCyc:   BSUB:BSU10250-MONOMER
- GO:   GO:0005737
- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562
- Gene3D:   G3DSA:3.30.390.50
- TIGRFAMs:   TIGR00545

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C

EC number: =2.7.7.63

Molecular weight: Translated: 38020; Mature: 38020

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: BINDING 69-69 BINDING 131-131 BINDING 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFIDNQNINDPRINLAIEEYCVKHLDPEQQYLLFYVNQPSIIIGKNQNTIEEINTKYVE
CEEECCCCCCCCEEEEEHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCHHHHHHHHEEE
ENGIIVVRRLSGGGAVYHDLGNLNFSFITKDDGDSFHNFKKFTEPVIQALHQLGVEAELS
CCCEEEEEEECCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEC
GRNDIVVDGRKISGNAQFATKGRIFSHGTLMFDSAIDHVVSALKVKKDKIESKGIKSIRS
CCCCEEEECEEECCCCEEEECCEEEECCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVANISEFLDDKMTTEEFRSHLLRHIFNTNDVGNVPEYKLTEKDWETIHQISKERYQNWD
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
WNYGRSPKFNLNHSKRYPVGSIDLHLEVKKGKIEDCKIFGDFFGVGDVSEIENLLVGKQY
CCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHEEEHHHCCCCCHHHHHHHHHCCHH
ERSVIADVLEGVNLKHYFGNITKEDFLDLIY
HHHHHHHHHHCCCCHHHHCCCCHHHHHHHCC
>Mature Secondary Structure
MLFIDNQNINDPRINLAIEEYCVKHLDPEQQYLLFYVNQPSIIIGKNQNTIEEINTKYVE
CEEECCCCCCCCEEEEEHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCHHHHHHHHEEE
ENGIIVVRRLSGGGAVYHDLGNLNFSFITKDDGDSFHNFKKFTEPVIQALHQLGVEAELS
CCCEEEEEEECCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEC
GRNDIVVDGRKISGNAQFATKGRIFSHGTLMFDSAIDHVVSALKVKKDKIESKGIKSIRS
CCCCEEEECEEECCCCEEEECCEEEECCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVANISEFLDDKMTTEEFRSHLLRHIFNTNDVGNVPEYKLTEKDWETIHQISKERYQNWD
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
WNYGRSPKFNLNHSKRYPVGSIDLHLEVKKGKIEDCKIFGDFFGVGDVSEIENLLVGKQY
CCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHEEEHHHCCCCCHHHHHHHHHCCHH
ERSVIADVLEGVNLKHYFGNITKEDFLDLIY
HHHHHHHHHHCCCCHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377