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The map label for this gene is yhfI

Identifier: 16078088

GI number: 16078088

Start: 1098412

End: 1099146

Strand: Direct

Name: yhfI

Synonym: BSU10240

Alternate gene names: 16078088

Gene position: 1098412-1099146 (Clockwise)

Preceding gene: 16078085

Following gene: 16078089

Centisome position: 26.06

GC content: 46.8

Gene sequence:

>735_bases
ATGAAAGTTACAGTTATCGGATGCTATGGCGGTTTTCCGGCCGCAAATGAAGCGACGTCAGGCTATTTGTTTCAGTCAGG
TGATTACTCTCTGCTTGTTGATTGCGGCAGTGCCGTATTGTCTAAGCTGTTCGGATATGTGCCGGCGGAAAAGCTGGATG
CGGTCGTTCTGTCTCATTATCACCATGACCATATCGCTGATATCGGACCGCTGCAATTTGCCAAGCAAGTCGGTTCGTTT
CTCGGCAAAGGAGAGCATACGCTTCCGATTTACGGACATGATGCTGATATAGAACAGTTTCAAAAGCTTACATATAAAAC
ACATACAAAAGGGATCGCCTATCAGCCGGATCAGCCGCTGACTGCCGGTCCGTTTACGATCACCTTTTTAAAAACGATTC
ACCCGGTGACGTGCTATGCCATGCGGATTACTGACGGCAGCCACACTGTCGTTTATACTGCTGATTCCAGCTATCAGGAT
TCATTTATACCGTTTTCGAAAGATGCTGATTTATTGATCTCAGAATGCAATTTTTATGCCGATCAAGACGGAACAAGTGC
AGGCCATATGAACAGCCTGGAAGCTGGGCGCATTGCAAAAGAAGCCGGAGCGGGAGAACTGCTGCTGACACATTTGCCGC
ATTTTGGCGTGCATGACAACCTTCGCAAAGAAGCGAAAACCGTTTTTAACGGAGAAGTGAATATTGCGAAATCAGGTTTT
GTGTGGGAAGGATAA

Upstream 100 bases:

>100_bases
ATCAATAACTGTTTTGATACAATGTTTAAAACATGCTTTTTTCAAGAAAAATGGGTATATTGAAGGAGGACCAATTTTTT
TGAAAAGATAGGAGGACATT

Downstream 100 bases:

>100_bases
AGGAGGAGCACCATGTTATTTATAGACAATCAAAATATCAATGATCCGCGGATCAATCTTGCTATCGAGGAGTACTGTGT
AAAGCATTTAGACCCTGAAC

Product: metal-dependent hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MKVTVIGCYGGFPAANEATSGYLFQSGDYSLLVDCGSAVLSKLFGYVPAEKLDAVVLSHYHHDHIADIGPLQFAKQVGSF
LGKGEHTLPIYGHDADIEQFQKLTYKTHTKGIAYQPDQPLTAGPFTITFLKTIHPVTCYAMRITDGSHTVVYTADSSYQD
SFIPFSKDADLLISECNFYADQDGTSAGHMNSLEAGRIAKEAGAGELLLTHLPHFGVHDNLRKEAKTVFNGEVNIAKSGF
VWEG

Sequences:

>Translated_244_residues
MKVTVIGCYGGFPAANEATSGYLFQSGDYSLLVDCGSAVLSKLFGYVPAEKLDAVVLSHYHHDHIADIGPLQFAKQVGSF
LGKGEHTLPIYGHDADIEQFQKLTYKTHTKGIAYQPDQPLTAGPFTITFLKTIHPVTCYAMRITDGSHTVVYTADSSYQD
SFIPFSKDADLLISECNFYADQDGTSAGHMNSLEAGRIAKEAGAGELLLTHLPHFGVHDNLRKEAKTVFNGEVNIAKSGF
VWEG
>Mature_244_residues
MKVTVIGCYGGFPAANEATSGYLFQSGDYSLLVDCGSAVLSKLFGYVPAEKLDAVVLSHYHHDHIADIGPLQFAKQVGSF
LGKGEHTLPIYGHDADIEQFQKLTYKTHTKGIAYQPDQPLTAGPFTITFLKTIHPVTCYAMRITDGSHTVVYTADSSYQD
SFIPFSKDADLLISECNFYADQDGTSAGHMNSLEAGRIAKEAGAGELLLTHLPHFGVHDNLRKEAKTVFNGEVNIAKSGF
VWEG

Specific function: Unknown

COG id: COG1234

COG function: function code R; Metal-dependent hydrolases of the beta-lactamase superfamily III

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YHFI_BACSU (O07607)

Other databases:

- EMBL:   Y14083
- EMBL:   AL009126
- PIR:   F69830
- RefSeq:   NP_388905.1
- HSSP:   Q81U06
- ProteinModelPortal:   O07607
- SMR:   O07607
- EnsemblBacteria:   EBBACT00000003230
- GeneID:   936318
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU10240
- NMPDR:   fig|224308.1.peg.1024
- GenoList:   BSU10240
- GeneTree:   EBGT00050000001790
- HOGENOM:   HBG728821
- OMA:   HHDHVAD
- ProtClustDB:   CLSK886999
- BioCyc:   BSUB:BSU10240-MONOMER
- InterPro:   IPR001279
- SMART:   SM00849

Pfam domain/function: PF00753 Lactamase_B

EC number: NA

Molecular weight: Translated: 26525; Mature: 26525

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVTVIGCYGGFPAANEATSGYLFQSGDYSLLVDCGSAVLSKLFGYVPAEKLDAVVLSHY
CEEEEEEECCCCCCCCCCCCCEEEECCCEEEEEECHHHHHHHHHCCCCHHHHHHHHHHHH
HHDHIADIGPLQFAKQVGSFLGKGEHTLPIYGHDADIEQFQKLTYKTHTKGIAYQPDQPL
CCCHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEECCCCCC
TAGPFTITFLKTIHPVTCYAMRITDGSHTVVYTADSSYQDSFIPFSKDADLLISECNFYA
CCCCEEEEHHHHCCCEEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCHHHEEECCCEEE
DQDGTSAGHMNSLEAGRIAKEAGAGELLLTHLPHFGVHDNLRKEAKTVFNGEVNIAKSGF
CCCCCCCCCCCCCCHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCC
VWEG
EECC
>Mature Secondary Structure
MKVTVIGCYGGFPAANEATSGYLFQSGDYSLLVDCGSAVLSKLFGYVPAEKLDAVVLSHY
CEEEEEEECCCCCCCCCCCCCEEEECCCEEEEEECHHHHHHHHHCCCCHHHHHHHHHHHH
HHDHIADIGPLQFAKQVGSFLGKGEHTLPIYGHDADIEQFQKLTYKTHTKGIAYQPDQPL
CCCHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEECCCCCC
TAGPFTITFLKTIHPVTCYAMRITDGSHTVVYTADSSYQDSFIPFSKDADLLISECNFYA
CCCCEEEEHHHHCCCEEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCHHHEEECCCEEE
DQDGTSAGHMNSLEAGRIAKEAGAGELLLTHLPHFGVHDNLRKEAKTVFNGEVNIAKSGF
CCCCCCCCCCCCCCHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCC
VWEG
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377