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The map label for this gene is yhfE

Identifier: 16078084

GI number: 16078084

Start: 1095063

End: 1096103

Strand: Direct

Name: yhfE

Synonym: BSU10200

Alternate gene names: 16078084

Gene position: 1095063-1096103 (Clockwise)

Preceding gene: 16078082

Following gene: 16078085

Centisome position: 25.98

GC content: 46.59

Gene sequence:

>1041_bases
ATGACGTCCGTACGTAAAACGATGGAGCTCATTAAAGAACTTGTATCGATCCCAAGCCCGACAGGAAATACATATGAGGT
GATCAATTATATTGAAAGCCTTTTAAAAGAATGGAAGGTAGAAACGGTTCGGAACCACAAGGGCGGCTTAATTGCAACGC
TTCCAGGACGCGACACATCCCGGCATCGCATGCTGACAGCGCACGTTGACACCCTCGGCGCTATGGTGAAGGAAATTAAA
GCGGACGGCCGGCTGAAAATCGACTTAATCGGCGGGTTCCGCTACAACTCGATTGAGGGGGAGTATTGCCAAATTGAAAC
CGCCTCGGGAAAAATGTACACAGGCACAATCTTAATGCATCAAACGTCTGTGCACGTATACAAAGACGCGGGAAAGGCTG
AGCGGAATCAAGAGAATATGGAGATCCGCCTAGATGAACCTGTTCACTGCCGGAAAGATACGGAGGAGCTTGGCATTGGC
GTCGGAGACTTTGTGTCGTTTGATCCACGTGTCGAGATCACATCAAGCGGATTTATTAAATCGCGCCATTTGGATGACAA
AGCAAGCGTCGCCTTATTGCTCCGCTTAATTCATGAAATTCAAACAGAAGACATTGAGCTGCCGTATACAACCCATTTTC
TTATTTCCAATAATGAAGAAATCGGTTACGGCGGGAATTCGAATATTCCTCCTGAAACAGTTGAATATTTGGCGGTTGAT
ATGGGCGCTATCGGTGACGGGCAGGCGACGGATGAATATTCAGTATCAATCTGTGTGAAGGATGCGAGCGGGCCGTATCA
TTATCAGCTCAGAAAGCATTTAGTTCAATTAGCCGAGAAGCATCATATTGACTATAAGCTTGATATATATCCGTACTACG
GGTCTGATGCGTCAGCAGCGATCAAATCCGGGCATGATATTGTGCATGGCCTCATAGGGCCGGGAATTGACGCTTCCCAT
GCATTTGAACGCACCCATAAATCATCTCTTCGCCATACCGCTAAGCTTCTGTATTACTATGTACAGTCACCAATGGTATA
A

Upstream 100 bases:

>100_bases
CATGCTCTCTACAGCATATGAGAGATTGATAGGAAAAGATTGGACGGGTCCCTCGGGAAAAGAGTATATTTTAAGTAGAA
AATAAAAGGAGGAATGCCCT

Downstream 100 bases:

>100_bases
GGGGGAGGACCAATGAAGATTTATAAGCTGAAAAGCACATTCGGTTGGGAAGGTGATGACGGGTTGGGAGAACAGCTGAT
TCAGCAGATCCTTGCCGGAG

Product: endoglucanase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MTSVRKTMELIKELVSIPSPTGNTYEVINYIESLLKEWKVETVRNHKGGLIATLPGRDTSRHRMLTAHVDTLGAMVKEIK
ADGRLKIDLIGGFRYNSIEGEYCQIETASGKMYTGTILMHQTSVHVYKDAGKAERNQENMEIRLDEPVHCRKDTEELGIG
VGDFVSFDPRVEITSSGFIKSRHLDDKASVALLLRLIHEIQTEDIELPYTTHFLISNNEEIGYGGNSNIPPETVEYLAVD
MGAIGDGQATDEYSVSICVKDASGPYHYQLRKHLVQLAEKHHIDYKLDIYPYYGSDASAAIKSGHDIVHGLIGPGIDASH
AFERTHKSSLRHTAKLLYYYVQSPMV

Sequences:

>Translated_346_residues
MTSVRKTMELIKELVSIPSPTGNTYEVINYIESLLKEWKVETVRNHKGGLIATLPGRDTSRHRMLTAHVDTLGAMVKEIK
ADGRLKIDLIGGFRYNSIEGEYCQIETASGKMYTGTILMHQTSVHVYKDAGKAERNQENMEIRLDEPVHCRKDTEELGIG
VGDFVSFDPRVEITSSGFIKSRHLDDKASVALLLRLIHEIQTEDIELPYTTHFLISNNEEIGYGGNSNIPPETVEYLAVD
MGAIGDGQATDEYSVSICVKDASGPYHYQLRKHLVQLAEKHHIDYKLDIYPYYGSDASAAIKSGHDIVHGLIGPGIDASH
AFERTHKSSLRHTAKLLYYYVQSPMV
>Mature_345_residues
TSVRKTMELIKELVSIPSPTGNTYEVINYIESLLKEWKVETVRNHKGGLIATLPGRDTSRHRMLTAHVDTLGAMVKEIKA
DGRLKIDLIGGFRYNSIEGEYCQIETASGKMYTGTILMHQTSVHVYKDAGKAERNQENMEIRLDEPVHCRKDTEELGIGV
GDFVSFDPRVEITSSGFIKSRHLDDKASVALLLRLIHEIQTEDIELPYTTHFLISNNEEIGYGGNSNIPPETVEYLAVDM
GAIGDGQATDEYSVSICVKDASGPYHYQLRKHLVQLAEKHHIDYKLDIYPYYGSDASAAIKSGHDIVHGLIGPGIDASHA
FERTHKSSLRHTAKLLYYYVQSPMV

Specific function: Unknown

COG id: COG1363

COG function: function code G; Cellulase M and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M42 family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YHFE_BACSU (O07603)

Other databases:

- EMBL:   Y14083
- EMBL:   AL009126
- PIR:   C69830
- RefSeq:   NP_388901.1
- ProteinModelPortal:   O07603
- SMR:   O07603
- EnsemblBacteria:   EBBACT00000001138
- GeneID:   936306
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU10200
- NMPDR:   fig|224308.1.peg.1020
- GenoList:   BSU10200
- GeneTree:   EBGT00050000001571
- HOGENOM:   HBG336725
- OMA:   PYDYHLS
- ProtClustDB:   CLSK865305
- BioCyc:   BSUB:BSU10200-MONOMER
- InterPro:   IPR008007
- PIRSF:   PIRSF001123

Pfam domain/function: PF05343 Peptidase_M42

EC number: NA

Molecular weight: Translated: 38736; Mature: 38605

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: ACT_SITE 219-219

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSVRKTMELIKELVSIPSPTGNTYEVINYIESLLKEWKVETVRNHKGGLIATLPGRDTS
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
RHRMLTAHVDTLGAMVKEIKADGRLKIDLIGGFRYNSIEGEYCQIETASGKMYTGTILMH
CCEEEEHHHHHHHHHHHHHCCCCCEEEEEEECEEECCCCCCEEEEEECCCCEEEEEEEEE
QTSVHVYKDAGKAERNQENMEIRLDEPVHCRKDTEELGIGVGDFVSFDPRVEITSSGFIK
ECEEEEEECCCCCCCCCCCCEEEECCCCCCCCCHHHHCCCCHHHCCCCCCEEEECCCCEE
SRHLDDKASVALLLRLIHEIQTEDIELPYTTHFLISNNEEIGYGGNSNIPPETVEYLAVD
ECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCEECCCCCCCCCHHHHHHHHHH
MGAIGDGQATDEYSVSICVKDASGPYHYQLRKHLVQLAEKHHIDYKLDIYPYYGSDASAA
CCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHH
IKSGHDIVHGLIGPGIDASHAFERTHKSSLRHTAKLLYYYVQSPMV
HHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TSVRKTMELIKELVSIPSPTGNTYEVINYIESLLKEWKVETVRNHKGGLIATLPGRDTS
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
RHRMLTAHVDTLGAMVKEIKADGRLKIDLIGGFRYNSIEGEYCQIETASGKMYTGTILMH
CCEEEEHHHHHHHHHHHHHCCCCCEEEEEEECEEECCCCCCEEEEEECCCCEEEEEEEEE
QTSVHVYKDAGKAERNQENMEIRLDEPVHCRKDTEELGIGVGDFVSFDPRVEITSSGFIK
ECEEEEEECCCCCCCCCCCCEEEECCCCCCCCCHHHHCCCCHHHCCCCCCEEEECCCCEE
SRHLDDKASVALLLRLIHEIQTEDIELPYTTHFLISNNEEIGYGGNSNIPPETVEYLAVD
ECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCEECCCCCCCCCHHHHHHHHHH
MGAIGDGQATDEYSVSICVKDASGPYHYQLRKHLVQLAEKHHIDYKLDIYPYYGSDASAA
CCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHH
IKSGHDIVHGLIGPGIDASHAFERTHKSSLRHTAKLLYYYVQSPMV
HHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377