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The map label for this gene is glpF

Identifier: 16077993

GI number: 16077993

Start: 1002501

End: 1003325

Strand: Direct

Name: glpF

Synonym: BSU09280

Alternate gene names: 16077993

Gene position: 1002501-1003325 (Clockwise)

Preceding gene: 16077992

Following gene: 255767217

Centisome position: 23.78

GC content: 46.3

Gene sequence:

>825_bases
ATGACAGCATTTTGGGGAGAAGTCATCGGTACGATGCTGCTTATCATTTTTGGTGCAGGTGTTTGTGCAGGTGTCAATTT
AAAGAAATCGCTTTCATTCCAGTCTGGCTGGATTGTTGTTGTATTTGGATGGGGATTGGGTGTTGCCATGGCGGCATACG
CGGTTGGCGGCATCAGCGGTGCCCATTTGAATCCGGCGCTAACGATAGCGCTTGCATTTGTAGGAGATTTTCCTTGGAAA
GAGGTTCCGGTTTATATTGCGGCGCAAATGATCGGAGCGATCATCGGTGCGGTGATTATTTATCTGCATTACCTCCCGCA
CTGGAAGTCAACGGATGATCCCGCTGCCAAGCTGGGTGTTTTCTCAACAGGGCCCAGCATTCCGCATACATTTGCAAACG
TTTTAAGCGAAGTGATTGGGACATTTGTCCTTGTACTTGGAATCTTGGCCATAGGTGCAAATCAATTTACAGAAGGACTT
AATCCTTTAATCGTCGGTTTCCTCATTGTAGCAATCGGTATTTCTCTGGGAGGCACCACCGGCTATGCTATCAATCCTGC
ACGTGACTTAGGTCCGCGGATCGCCCACGCTTTTCTTCCGATTCCGGGGAAGGGCTCATCAAACTGGAAATACGCATGGG
TTCCGGTAGTCGGCCCGATTTTAGGCGGATCATTCGGCGGTGTATTTTACAACGCTGCATTTAAAGGGCACATCACAAGC
AGCTTCTGGATTGTAAGCGTTATATTGGTTGTGGTATTGTTAGGACTCTATGTTTATACGAAATCACATTCTGCTAAAAC
ATTATCAAATTCTAAATATATTTAA

Upstream 100 bases:

>100_bases
ACGGCAAATGTTTATGCACCCGTAAAGCGGTTTGTTGTGGTTTTTTTATTCTCTTCTTCTCTATCATGCTTTTTAATCGT
GACTTTAGGAGGAATGTGCT

Downstream 100 bases:

>100_bases
TCAAAGGGGAGACATCTTATGGAAACGTACATTTTATCCTTAGATCAGGGGACGACAAGTTCAAGAGCGATTCTGTTTAA
TAAAGAAGGCAAAATTGTCC

Product: glycerol permease

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MTAFWGEVIGTMLLIIFGAGVCAGVNLKKSLSFQSGWIVVVFGWGLGVAMAAYAVGGISGAHLNPALTIALAFVGDFPWK
EVPVYIAAQMIGAIIGAVIIYLHYLPHWKSTDDPAAKLGVFSTGPSIPHTFANVLSEVIGTFVLVLGILAIGANQFTEGL
NPLIVGFLIVAIGISLGGTTGYAINPARDLGPRIAHAFLPIPGKGSSNWKYAWVPVVGPILGGSFGGVFYNAAFKGHITS
SFWIVSVILVVVLLGLYVYTKSHSAKTLSNSKYI

Sequences:

>Translated_274_residues
MTAFWGEVIGTMLLIIFGAGVCAGVNLKKSLSFQSGWIVVVFGWGLGVAMAAYAVGGISGAHLNPALTIALAFVGDFPWK
EVPVYIAAQMIGAIIGAVIIYLHYLPHWKSTDDPAAKLGVFSTGPSIPHTFANVLSEVIGTFVLVLGILAIGANQFTEGL
NPLIVGFLIVAIGISLGGTTGYAINPARDLGPRIAHAFLPIPGKGSSNWKYAWVPVVGPILGGSFGGVFYNAAFKGHITS
SFWIVSVILVVVLLGLYVYTKSHSAKTLSNSKYI
>Mature_273_residues
TAFWGEVIGTMLLIIFGAGVCAGVNLKKSLSFQSGWIVVVFGWGLGVAMAAYAVGGISGAHLNPALTIALAFVGDFPWKE
VPVYIAAQMIGAIIGAVIIYLHYLPHWKSTDDPAAKLGVFSTGPSIPHTFANVLSEVIGTFVLVLGILAIGANQFTEGLN
PLIVGFLIVAIGISLGGTTGYAINPARDLGPRIAHAFLPIPGKGSSNWKYAWVPVVGPILGGSFGGVFYNAAFKGHITSS
FWIVSVILVVVLLGLYVYTKSHSAKTLSNSKYI

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family

Homologues:

Organism=Homo sapiens, GI4826645, Length=238, Percent_Identity=37.3949579831933, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI4502187, Length=243, Percent_Identity=37.037037037037, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI157266307, Length=239, Percent_Identity=34.3096234309623, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI22538420, Length=240, Percent_Identity=32.9166666666667, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI310133356, Length=187, Percent_Identity=33.1550802139037, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI310114181, Length=187, Percent_Identity=33.1550802139037, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI4502179, Length=234, Percent_Identity=32.0512820512821, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI4502181, Length=204, Percent_Identity=31.8627450980392, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI4755125, Length=204, Percent_Identity=31.8627450980392, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI6912506, Length=241, Percent_Identity=30.2904564315353, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1790362, Length=259, Percent_Identity=37.0656370656371, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1787101, Length=160, Percent_Identity=35.625, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71994009, Length=246, Percent_Identity=32.520325203252, Blast_Score=117, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI71992966, Length=240, Percent_Identity=31.25, Blast_Score=107, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17533613, Length=243, Percent_Identity=31.6872427983539, Blast_Score=103, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17544068, Length=256, Percent_Identity=33.203125, Blast_Score=103, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI32564052, Length=243, Percent_Identity=31.6872427983539, Blast_Score=103, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17531431, Length=237, Percent_Identity=30.8016877637131, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17531429, Length=237, Percent_Identity=30.8016877637131, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17558372, Length=225, Percent_Identity=27.5555555555556, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71992961, Length=238, Percent_Identity=29.4117647058824, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71993722, Length=232, Percent_Identity=28.448275862069, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321054, Length=209, Percent_Identity=39.7129186602871, Blast_Score=129, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6322985, Length=330, Percent_Identity=28.7878787878788, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24652747, Length=234, Percent_Identity=35.042735042735, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI45551084, Length=234, Percent_Identity=35.042735042735, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24762342, Length=247, Percent_Identity=28.3400809716599, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24762344, Length=250, Percent_Identity=27.6, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24762346, Length=250, Percent_Identity=27.6, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24762348, Length=250, Percent_Identity=27.6, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI20130305, Length=250, Percent_Identity=27.6, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLPF_BACSU (P18156)

Other databases:

- EMBL:   M99611
- EMBL:   Y14079
- EMBL:   AL009126
- EMBL:   M34393
- PIR:   C47700
- RefSeq:   NP_388809.1
- ProteinModelPortal:   P18156
- SMR:   P18156
- EnsemblBacteria:   EBBACT00000003509
- GeneID:   939266
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU09280
- NMPDR:   fig|224308.1.peg.928
- GenoList:   BSU09280
- GeneTree:   EBGT00050000002004
- HOGENOM:   HBG705794
- OMA:   GDLPWAS
- PhylomeDB:   P18156
- ProtClustDB:   CLSK886948
- BioCyc:   BSUB:BSU09280-MONOMER
- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357
- PANTHER:   PTHR19139
- PRINTS:   PR00783
- TIGRFAMs:   TIGR00861

Pfam domain/function: PF00230 MIP

EC number: NA

Molecular weight: Translated: 28735; Mature: 28604

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xd53aea0)-; HASH(0xc407fa4)-; HASH(0xdec94d4)-; HASH(0xdf0f228)-; HASH(0xd1cb188)-; HASH(0xdf8c5fc)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAFWGEVIGTMLLIIFGAGVCAGVNLKKSLSFQSGWIVVVFGWGLGVAMAAYAVGGISG
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCEEEEECHHHHHHHHHHHHCCCCC
AHLNPALTIALAFVGDFPWKEVPVYIAAQMIGAIIGAVIIYLHYLPHWKSTDDPAAKLGV
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCC
FSTGPSIPHTFANVLSEVIGTFVLVLGILAIGANQFTEGLNPLIVGFLIVAIGISLGGTT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHCCCCC
GYAINPARDLGPRIAHAFLPIPGKGSSNWKYAWVPVVGPILGGSFGGVFYNAAFKGHITS
CCEECCHHHHCHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHCCCCCHHHHHHECCCCCCH
SFWIVSVILVVVLLGLYVYTKSHSAKTLSNSKYI
HHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCH
>Mature Secondary Structure 
TAFWGEVIGTMLLIIFGAGVCAGVNLKKSLSFQSGWIVVVFGWGLGVAMAAYAVGGISG
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCEEEEECHHHHHHHHHHHHCCCCC
AHLNPALTIALAFVGDFPWKEVPVYIAAQMIGAIIGAVIIYLHYLPHWKSTDDPAAKLGV
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCC
FSTGPSIPHTFANVLSEVIGTFVLVLGILAIGANQFTEGLNPLIVGFLIVAIGISLGGTT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHCCCCC
GYAINPARDLGPRIAHAFLPIPGKGSSNWKYAWVPVVGPILGGSFGGVFYNAAFKGHITS
CCEECCHHHHCHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHCCCCCHHHHHHECCCCCCH
SFWIVSVILVVVLLGLYVYTKSHSAKTLSNSKYI
HHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8436953; 9579061; 9384377; 2127799