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The map label for this gene is tcyP

Identifier: 16077978

GI number: 16077978

Start: 986986

End: 988377

Strand: Direct

Name: tcyP

Synonym: BSU09130

Alternate gene names: 16077978

Gene position: 986986-988377 (Clockwise)

Preceding gene: 255767210

Following gene: 255767211

Centisome position: 23.41

GC content: 45.91

Gene sequence:

>1392_bases
TTGGAGACTTTACTGGTTGTATTACATGTATTTATTTTATTTTTACTTATCTTAGGGCTCTTTGTTATGCAGAAAAAACA
TGTTTCCTTCTCTAAACGTGTATTTACTGCGCTGGGGCTGGGGATTGTATTCGGATTTGCGCTTCAGCTGATTTACGGCC
CGACTTCAAATATAGTCATTCAAACAGCCGACTGGTTTAATATTGCCGGGGGCGGATATGTCAAATTGCTTCAGATGGTT
GTCATGCCGCTCGTCTTTATTTCGATTCTCGGCGCCTTTACAAAGCTGAAGCTGACAAAAAATCTTGGGAAAATCAGCGG
TTTGATTATCGGGATTTTAGTAGCAACAACAGCAGTTGCTGCAGCTGTCGGTATCGCGTCCGCGCTTTCATTTGATCTGC
AGGCGATTCAGGTTGACCAAGGAAGTACAGAGCTTTCACGGGGACAGGAGCTTCAGCAGAAATCAGAGGATATGACAGCG
AAAACGCTGCCGCAGCAGATTGTTGAACTGCTGCCTGGAAACCCGTTTTTAGATTTTACGGGAGCTAGACCGACTTCAAC
GATCGCTGTCGTTATTTTTGCCGCTTTCCTTGGAGTGGCATTCCTCGGCGTAAAACACAAACAGCCGGAACAGGCAGAAA
CATTTAAAAAACTGGTTGATGCCGTTTATGCCATTGTCATGCGTGTTGTCACACTCATTTTGAGACTGACGCCTTATGGG
GTGCTGGCCATCATGACAAAAACAATTGCAACGAGTGATCTTGACAGCATTCTAAAACTGGGCATGTTCGTGATTGCATC
ATATGCCGCGTTAATCACTATGTTTATCATTCATTTGCTGCTGCTGACGTTCAGCGGCTTGAATCCGGTGATTTATTTGA
AAAAAGCGGTGCCTGTACTTGTATTTGCATTTACGTCAAGGTCTAGCGCAGGTGCTTTACCGCTGAATATTAAAACACAG
AGAAGCATGGGTGTACCGGAGGGAATCGCAAACTTTGCCGGTTCGTTCGGTCTTTCGATCGGCCAAAATGGATGTGCCGG
CATTTATCCTGCGATGCTGGCGATGATGATTGCTCCGACTGTCGGCCAAAATCCATTTGACCCGGTGTTTATTATCACTG
TTATCGCAGTCGTTGCCATCAGCTCCTTCGGGGTTGCCGGAGTCGGCGGCGGAGCGACATTCGCGGCGCTTCTTGTTTTA
TCATCATTAAACATGCCTGTCGCGCTCGCCGGTTTGTTAATCTCAATTGAACCGTTAATCGACATGGGGCGTACTGCTTT
GAATGTCAGCGGAAGCATGACATCCGGTCTTATTACAAGTAAGGTCACAAAAGAAATTGATCAAGGGGCATTTCATGATC
AATCCAGAGTGATTGAAGCTGAAGAAGCGTAA

Upstream 100 bases:

>100_bases
TTTTTTACTTGAATTGATAAGTTAAAATAATTCGCACTTGACCCATCGGAATTAGTGTAATATAGTAAATGAGAAAATTT
GAAGGGAGAGTTTACCTGCT

Downstream 100 bases:

>100_bases
CATATGAAAACGTGTAATCCAAGAGGATTACACGTTTTTTTAACGGCCAGGCTCTAAGTCCTCCAGCATTTCCATCATCG
TGTTGTCGTCCATGACATTC

Product: sodium-cystine symporter

Products: NA

Alternate protein names: Symporter yhcL; Transporter of cystine tcyP

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
METLLVVLHVFILFLLILGLFVMQKKHVSFSKRVFTALGLGIVFGFALQLIYGPTSNIVIQTADWFNIAGGGYVKLLQMV
VMPLVFISILGAFTKLKLTKNLGKISGLIIGILVATTAVAAAVGIASALSFDLQAIQVDQGSTELSRGQELQQKSEDMTA
KTLPQQIVELLPGNPFLDFTGARPTSTIAVVIFAAFLGVAFLGVKHKQPEQAETFKKLVDAVYAIVMRVVTLILRLTPYG
VLAIMTKTIATSDLDSILKLGMFVIASYAALITMFIIHLLLLTFSGLNPVIYLKKAVPVLVFAFTSRSSAGALPLNIKTQ
RSMGVPEGIANFAGSFGLSIGQNGCAGIYPAMLAMMIAPTVGQNPFDPVFIITVIAVVAISSFGVAGVGGGATFAALLVL
SSLNMPVALAGLLISIEPLIDMGRTALNVSGSMTSGLITSKVTKEIDQGAFHDQSRVIEAEEA

Sequences:

>Translated_463_residues
METLLVVLHVFILFLLILGLFVMQKKHVSFSKRVFTALGLGIVFGFALQLIYGPTSNIVIQTADWFNIAGGGYVKLLQMV
VMPLVFISILGAFTKLKLTKNLGKISGLIIGILVATTAVAAAVGIASALSFDLQAIQVDQGSTELSRGQELQQKSEDMTA
KTLPQQIVELLPGNPFLDFTGARPTSTIAVVIFAAFLGVAFLGVKHKQPEQAETFKKLVDAVYAIVMRVVTLILRLTPYG
VLAIMTKTIATSDLDSILKLGMFVIASYAALITMFIIHLLLLTFSGLNPVIYLKKAVPVLVFAFTSRSSAGALPLNIKTQ
RSMGVPEGIANFAGSFGLSIGQNGCAGIYPAMLAMMIAPTVGQNPFDPVFIITVIAVVAISSFGVAGVGGGATFAALLVL
SSLNMPVALAGLLISIEPLIDMGRTALNVSGSMTSGLITSKVTKEIDQGAFHDQSRVIEAEEA
>Mature_463_residues
METLLVVLHVFILFLLILGLFVMQKKHVSFSKRVFTALGLGIVFGFALQLIYGPTSNIVIQTADWFNIAGGGYVKLLQMV
VMPLVFISILGAFTKLKLTKNLGKISGLIIGILVATTAVAAAVGIASALSFDLQAIQVDQGSTELSRGQELQQKSEDMTA
KTLPQQIVELLPGNPFLDFTGARPTSTIAVVIFAAFLGVAFLGVKHKQPEQAETFKKLVDAVYAIVMRVVTLILRLTPYG
VLAIMTKTIATSDLDSILKLGMFVIASYAALITMFIIHLLLLTFSGLNPVIYLKKAVPVLVFAFTSRSSAGALPLNIKTQ
RSMGVPEGIANFAGSFGLSIGQNGCAGIYPAMLAMMIAPTVGQNPFDPVFIITVIAVVAISSFGVAGVGGGATFAALLVL
SSLNMPVALAGLLISIEPLIDMGRTALNVSGSMTSGLITSKVTKEIDQGAFHDQSRVIEAEEA

Specific function: Mediates uptake of L-cystine, the oxidized form of L- cysteine. Although it is more specific for L-cystine, it could also transport a much broader range of amino acids and sulfur compounds including S-methylcysteine

COG id: COG1823

COG function: function code R; Predicted Na+/dicarboxylate symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family

Homologues:

Organism=Homo sapiens, GI223468564, Length=282, Percent_Identity=26.241134751773, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI223468566, Length=280, Percent_Identity=26.4285714285714, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI5032093, Length=411, Percent_Identity=24.8175182481752, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI169790839, Length=341, Percent_Identity=25.5131964809384, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI40254478, Length=262, Percent_Identity=28.2442748091603, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI4827012, Length=268, Percent_Identity=25, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI194239697, Length=305, Percent_Identity=23.9344262295082, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788024, Length=463, Percent_Identity=50.755939524838, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI1790514, Length=427, Percent_Identity=24.1217798594848, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1789947, Length=437, Percent_Identity=21.9679633867277, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71983106, Length=458, Percent_Identity=23.1441048034935, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI193206505, Length=460, Percent_Identity=22.3913043478261, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71983099, Length=458, Percent_Identity=23.1441048034935, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI193206654, Length=470, Percent_Identity=21.7021276595745, Blast_Score=75, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17537407, Length=441, Percent_Identity=22.6757369614512, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71996953, Length=465, Percent_Identity=22.7956989247312, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24583025, Length=385, Percent_Identity=24.1558441558442, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17137668, Length=385, Percent_Identity=24.1558441558442, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24583023, Length=385, Percent_Identity=24.1558441558442, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TCYP_BACSU (P54596)

Other databases:

- EMBL:   X96983
- EMBL:   AL009126
- PIR:   H69822
- RefSeq:   NP_388794.1
- ProteinModelPortal:   P54596
- SMR:   P54596
- EnsemblBacteria:   EBBACT00000001459
- GeneID:   939257
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU09130
- NMPDR:   fig|224308.1.peg.913
- GenoList:   BSU09130
- GeneTree:   EBGT00070000032022
- HOGENOM:   HBG739804
- OMA:   AWVMRLV
- PhylomeDB:   P54596
- ProtClustDB:   CLSK2518215
- BioCyc:   BSUB:BSU09130-MONOMER
- InterPro:   IPR001991
- PANTHER:   PTHR11958
- PRINTS:   PR00173

Pfam domain/function: PF00375 SDF

EC number: NA

Molecular weight: Translated: 48983; Mature: 48983

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xcd01ec0)-; HASH(0xbd20658)-; HASH(0xd3d0f4c)-; HASH(0xd773a58)-; HASH(0xcf1f920)-; HASH(0xdb9c978)-; HASH(0xd6eda38)-; HASH(0xd8add10)-; HASH(0xde72150)-; HASH(0xde6d66c)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCHHEECCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCH
>Mature Secondary Structure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HCCCHHEECCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377