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The map label for this gene is yfiS

Identifier: 16077905

GI number: 16077905

Start: 912547

End: 913800

Strand: Reverse

Name: yfiS

Synonym: BSU08380

Alternate gene names: 16077905

Gene position: 913800-912547 (Counterclockwise)

Preceding gene: 16077907

Following gene: 16077904

Centisome position: 21.68

GC content: 47.21

Gene sequence:

>1254_bases
ATGGAAAAACCGTTGTTTCGCAATCAAAAAGGACTGATGACACTGTTAGCCTCGCAAACCATTTCTTCACTTGGAGACTG
GCTTCACATTTTGGCCGTGCTAACATTAGCCGCTTTTCAGCTTCATGCTTCTCCATTAGATATGTCGCTTTTAATGATGT
CGTTTGCTTTGCCTGTCATTGTACTCGGACCTGTATCGGGTCTGCTTGCCGACCGTTTTGACAGAAAAACGATCATGTTT
CTATCTGAAATCGGCCGTGCCCTCACTGTTATCAGCTGCGTTTATGTCAGTGAGCTTTGGCAGCTTTACGTATTGCTATC
CGTTCAGAGCTGTTTTTCTTCACTTTTTTTACCTGCTAAAAACGGAAAGCTTAAGGAATTGGCTCCGGAAGCACACATTC
AGCAGGCCGTTTCCGTCAGCAGCATCATTGATAACAGCTCAAAAATCTTCGGCCCTGCCCTTGGCGGCACGCTGATCGCT
GCTTTCTCGATACACTCTGTTTTTTATATAAACGCCGGCGCCTTCTTTCTTTCCGCTGTCATTTTGTTTTTCCTTCCCAG
AGATGCTTTTTTACAAAAAGCCAATACACCTCAAGAAAAAACAGCAGCACTTACTTCTATCAAGGAAGGCCTTCAGTTTT
TGAAACGGATGCCGCTTCTATTAACAGGCTTACTAACAGCTTGTGTCGTTCTCTTTGTTTTGCAGATTGGTGATTCCCAA
GCGATTATTCTTATCCGCTCCTTTTCAGGAGCTCCGCCCGAGCTGGCAGGCTGGTGCATGGCCGTAAGCGGAGCCGGGAT
GCTCCTTACAGCGGCCATTACCGGCAGACGGCGAATCACTTCTTATTTGCTTTATTTCTCAGCTGGAACCCTTCTTCTTG
GACTAGCAACTGGAGGTGCGCCATTCCTAAGCGGTATGGGAATTGCCGGCATCACTCTCTTTATATTTGCTTTTTTCATC
ATGGGGGCGGCTTTCGGCCTCGTCCATATTCCATTTCAGATTCTCGTGCAAACAACAGTGCCTGTCGATTACAGCGGTAG
GGTGTTCGGCGCTATTCAGAGTGCGACAACCCTCGCCTCTATCCTCGGCATGGCGGGCGGGGGCGTGCTGGCGGAATGGA
TCGGTGTATCACTTGCTTTTCTTGTCTGTGGCTGCTTGCTGATCATGATTGGGTTGATTACACTTATAGGTAAGAAAATT
GCAGAAAGCAGGCGATATCTTGTCACCAAAAGTAACAAAGGAGCACAAGGATAA

Upstream 100 bases:

>100_bases
CTTCTCCTTTATTTTACAAGAAAAAGATTGTATATTGTGTCTTTTTCCCATACAATTAAAAACGACACTAGTGTCATTTT
AATAATCAGGAGGATGAATC

Downstream 100 bases:

>100_bases
ACGGCAGGCAGAGATTTTAGAGGCTGCCAAAACGGTTTTTAAACGAAAAGGATTCGAATTAACGACGATGAAGGATGTTG
TAGAAGAGTCAGGTTTCAGC

Product: efflux transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MEKPLFRNQKGLMTLLASQTISSLGDWLHILAVLTLAAFQLHASPLDMSLLMMSFALPVIVLGPVSGLLADRFDRKTIMF
LSEIGRALTVISCVYVSELWQLYVLLSVQSCFSSLFLPAKNGKLKELAPEAHIQQAVSVSSIIDNSSKIFGPALGGTLIA
AFSIHSVFYINAGAFFLSAVILFFLPRDAFLQKANTPQEKTAALTSIKEGLQFLKRMPLLLTGLLTACVVLFVLQIGDSQ
AIILIRSFSGAPPELAGWCMAVSGAGMLLTAAITGRRRITSYLLYFSAGTLLLGLATGGAPFLSGMGIAGITLFIFAFFI
MGAAFGLVHIPFQILVQTTVPVDYSGRVFGAIQSATTLASILGMAGGGVLAEWIGVSLAFLVCGCLLIMIGLITLIGKKI
AESRRYLVTKSNKGAQG

Sequences:

>Translated_417_residues
MEKPLFRNQKGLMTLLASQTISSLGDWLHILAVLTLAAFQLHASPLDMSLLMMSFALPVIVLGPVSGLLADRFDRKTIMF
LSEIGRALTVISCVYVSELWQLYVLLSVQSCFSSLFLPAKNGKLKELAPEAHIQQAVSVSSIIDNSSKIFGPALGGTLIA
AFSIHSVFYINAGAFFLSAVILFFLPRDAFLQKANTPQEKTAALTSIKEGLQFLKRMPLLLTGLLTACVVLFVLQIGDSQ
AIILIRSFSGAPPELAGWCMAVSGAGMLLTAAITGRRRITSYLLYFSAGTLLLGLATGGAPFLSGMGIAGITLFIFAFFI
MGAAFGLVHIPFQILVQTTVPVDYSGRVFGAIQSATTLASILGMAGGGVLAEWIGVSLAFLVCGCLLIMIGLITLIGKKI
AESRRYLVTKSNKGAQG
>Mature_417_residues
MEKPLFRNQKGLMTLLASQTISSLGDWLHILAVLTLAAFQLHASPLDMSLLMMSFALPVIVLGPVSGLLADRFDRKTIMF
LSEIGRALTVISCVYVSELWQLYVLLSVQSCFSSLFLPAKNGKLKELAPEAHIQQAVSVSSIIDNSSKIFGPALGGTLIA
AFSIHSVFYINAGAFFLSAVILFFLPRDAFLQKANTPQEKTAALTSIKEGLQFLKRMPLLLTGLLTACVVLFVLQIGDSQ
AIILIRSFSGAPPELAGWCMAVSGAGMLLTAAITGRRRITSYLLYFSAGTLLLGLATGGAPFLSGMGIAGITLFIFAFFI
MGAAFGLVHIPFQILVQTTVPVDYSGRVFGAIQSATTLASILGMAGGGVLAEWIGVSLAFLVCGCLLIMIGLITLIGKKI
AESRRYLVTKSNKGAQG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YFIS_BACSU (O31561)

Other databases:

- EMBL:   D85082
- EMBL:   AL009126
- PIR:   E69804
- RefSeq:   NP_388719.1
- ProteinModelPortal:   O31561
- EnsemblBacteria:   EBBACT00000003933
- GeneID:   939219
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU08380
- NMPDR:   fig|224308.1.peg.838
- GenoList:   BSU08380
- GeneTree:   EBGT00070000031742
- BioCyc:   BSUB:BSU08380-MONOMER
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 44397; Mature: 44397

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xce9e578)-; HASH(0xd10a470)-; HASH(0xd590670)-; HASH(0xcf27cc0)-; HASH(0xd5081cc)-; HASH(0xce09340)-; HASH(0xdd2dc34)-; HASH(0xdbcd918)-; HASH(0xd5055b0)-; HASH(0xd751098)-;

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKPLFRNQKGLMTLLASQTISSLGDWLHILAVLTLAAFQLHASPLDMSLLMMSFALPVI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VLGPVSGLLADRFDRKTIMFLSEIGRALTVISCVYVSELWQLYVLLSVQSCFSSLFLPAK
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NGKLKELAPEAHIQQAVSVSSIIDNSSKIFGPALGGTLIAAFSIHSVFYINAGAFFLSAV
CCCHHHHCHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHEEEEHHHHHHHHH
ILFFLPRDAFLQKANTPQEKTAALTSIKEGLQFLKRMPLLLTGLLTACVVLFVLQIGDSQ
HHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AIILIRSFSGAPPELAGWCMAVSGAGMLLTAAITGRRRITSYLLYFSAGTLLLGLATGGA
EEEEEEECCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHEEECCCCC
PFLSGMGIAGITLFIFAFFIMGAAFGLVHIPFQILVQTTVPVDYSGRVFGAIQSATTLAS
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHH
ILGMAGGGVLAEWIGVSLAFLVCGCLLIMIGLITLIGKKIAESRRYLVTKSNKGAQG
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCH
>Mature Secondary Structure
MEKPLFRNQKGLMTLLASQTISSLGDWLHILAVLTLAAFQLHASPLDMSLLMMSFALPVI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VLGPVSGLLADRFDRKTIMFLSEIGRALTVISCVYVSELWQLYVLLSVQSCFSSLFLPAK
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NGKLKELAPEAHIQQAVSVSSIIDNSSKIFGPALGGTLIAAFSIHSVFYINAGAFFLSAV
CCCHHHHCHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHEEEEHHHHHHHHH
ILFFLPRDAFLQKANTPQEKTAALTSIKEGLQFLKRMPLLLTGLLTACVVLFVLQIGDSQ
HHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AIILIRSFSGAPPELAGWCMAVSGAGMLLTAAITGRRRITSYLLYFSAGTLLLGLATGGA
EEEEEEECCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHEEECCCCC
PFLSGMGIAGITLFIFAFFIMGAAFGLVHIPFQILVQTTVPVDYSGRVFGAIQSATTLAS
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHH
ILGMAGGGVLAEWIGVSLAFLVCGCLLIMIGLITLIGKKIAESRRYLVTKSNKGAQG
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377