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The map label for this gene is yfiU

Identifier: 16077907

GI number: 16077907

Start: 914457

End: 916013

Strand: Reverse

Name: yfiU

Synonym: BSU08400

Alternate gene names: 16077907

Gene position: 916013-914457 (Counterclockwise)

Preceding gene: 16077908

Following gene: 16077905

Centisome position: 21.73

GC content: 49.07

Gene sequence:

>1557_bases
ATGTCTGAAGCATTAACAGAGCAGAAAAGCCAAAAGTGGGCAATCAGCCTGTTTACGATTGGCGTGTTTATGGCTGCTTT
AGATAACGGAATTATTTCGGCTGCTTTAACAACGATAAACGAGTCGTTTTCCGTTTCCCCTTCCTGGGGATCATGGGGCA
TTACCCTTTATACGCTCGGTCTTAGCGTCAGTGTGCCAATCGTCGGAAAACTTTCCGACCGCTACGGCAGAAAAAAACTC
TTTCTCATTGAGGTTTGCCTGTTCGGACTTGGTTCTTTGCTTGTTGCTTTGAGCCAAAGCTTCCCGCTCTTTCTGATATC
GCGGCTGATTCAGGCGCTTGGAGGCGGCGGGATTTTCATCATCGGCAGCTCTCACATTCTCGCGACCCTTCCAAAGGAAA
AACAGGGGAAGGCGCTTGGGCTGCTTGGCGCAATGAACGGGATGGCCGCTGTGCTCGGGCCAAATATCGGAAGCTTTCTG
CTTGATTGGACCGGGTCTTGGCATTGGCTATTTTTGATTAATTTGCCGATTGCCGTGCTATTGGTTGTTTTCGGCGCATG
TTTTATCGCGGAAACAAAAGCACCTGAGGCTAAGCGCCTCGATGCGGCCGGAATCTTCCTTTTGTCCCTGTCGATTTTAG
CTGTGATGTACGGCATGACCAATCTTGACGGGGCAAATCTCTTACACAGCTTGGGGAATCCGGAGGTTTATGGATGTATT
ATTTTTGGCATACTCTGTTTTGCAGCGCTTATCTCGTATGAAAAGAGAGTTGAAATGCGAGGAGGCGATCCCATTTTGGC
GTATTCTCTGCTGCGAAACCATATGTTTCAGCGCACGCTTATCATCGGTTTGTTATCCGGGGGGCTCTTGGCGGCAGTCA
TTTTTATCCCGTCATATGTCGAACAGTATTTGGGTGTCCCTGCCGCAAAAGCAGGCTATTGGATGACACCGCTTGCGTTG
GCTTCCGGAATCGGCGCTTGGCTTGGCGGAGCACTTACCGACAAAAAAGGTCCTGTAAAAACCGTTATTCTTTCGGGAAT
CATCTCATGTGCGGGGTTTGCTTTATTTCCGCTCTGGGTCACGGAAAAATGGGAGTTTGTGATCGCAAGTGTGGCAGCTG
GGATCGGTTTTGGCTTTCTGTTAGGCGCTCCGCTGAACGTCCTTGTATCAGAAGCGGCAAAAACAAATAAGGGTACAGCT
CTTGGCACGCTTTCATTAGTAAGGCAAATTGGACTGACCTTGGCGCCTACTCTTTATGCAGGTTTTATCACAGCCGGGTT
TGACCAAATAGGTGATGAGATCAACAGCCGCTTATCGGACAGCGGATATTCAGAAAAGGCGATGCAGATGATTCCGGAGA
TCGACAGTTCTGAAGTTTCTTCTTTACAAGAACAAATCGAACGCATTCCCGTTCCTGAAGTCAAAACCGCGATAAGCGAC
GCCATTCATGCGAGTGTCGCCAGCGGCTATGATCATTTATATGCAGCGGCTGCTGTGGTCTCACTTCTGGTGATTGCTGC
TATCAGTATTCCGGCTTTCCGCCGGCAAAAGCGTTAA

Upstream 100 bases:

>100_bases
CTGTCATCCGCAAACTGCGGGAGCAGGCTTTTTTTATTTGACATTTAGTTCATTTAAAATGAATAATATATTTCAAGTGA
ACTAAAAGGAGTTGACGAAA

Downstream 100 bases:

>100_bases
GACCACCCCATCCGTCTGGAAAGCTCCGTAATATGAGCGATATGGTGATGTGAGTGCCAGACATACAGGCCGAGCGCGTT
CTCCAGGGTAATGATCTCCT

Product: efflux transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MSEALTEQKSQKWAISLFTIGVFMAALDNGIISAALTTINESFSVSPSWGSWGITLYTLGLSVSVPIVGKLSDRYGRKKL
FLIEVCLFGLGSLLVALSQSFPLFLISRLIQALGGGGIFIIGSSHILATLPKEKQGKALGLLGAMNGMAAVLGPNIGSFL
LDWTGSWHWLFLINLPIAVLLVVFGACFIAETKAPEAKRLDAAGIFLLSLSILAVMYGMTNLDGANLLHSLGNPEVYGCI
IFGILCFAALISYEKRVEMRGGDPILAYSLLRNHMFQRTLIIGLLSGGLLAAVIFIPSYVEQYLGVPAAKAGYWMTPLAL
ASGIGAWLGGALTDKKGPVKTVILSGIISCAGFALFPLWVTEKWEFVIASVAAGIGFGFLLGAPLNVLVSEAAKTNKGTA
LGTLSLVRQIGLTLAPTLYAGFITAGFDQIGDEINSRLSDSGYSEKAMQMIPEIDSSEVSSLQEQIERIPVPEVKTAISD
AIHASVASGYDHLYAAAAVVSLLVIAAISIPAFRRQKR

Sequences:

>Translated_518_residues
MSEALTEQKSQKWAISLFTIGVFMAALDNGIISAALTTINESFSVSPSWGSWGITLYTLGLSVSVPIVGKLSDRYGRKKL
FLIEVCLFGLGSLLVALSQSFPLFLISRLIQALGGGGIFIIGSSHILATLPKEKQGKALGLLGAMNGMAAVLGPNIGSFL
LDWTGSWHWLFLINLPIAVLLVVFGACFIAETKAPEAKRLDAAGIFLLSLSILAVMYGMTNLDGANLLHSLGNPEVYGCI
IFGILCFAALISYEKRVEMRGGDPILAYSLLRNHMFQRTLIIGLLSGGLLAAVIFIPSYVEQYLGVPAAKAGYWMTPLAL
ASGIGAWLGGALTDKKGPVKTVILSGIISCAGFALFPLWVTEKWEFVIASVAAGIGFGFLLGAPLNVLVSEAAKTNKGTA
LGTLSLVRQIGLTLAPTLYAGFITAGFDQIGDEINSRLSDSGYSEKAMQMIPEIDSSEVSSLQEQIERIPVPEVKTAISD
AIHASVASGYDHLYAAAAVVSLLVIAAISIPAFRRQKR
>Mature_517_residues
SEALTEQKSQKWAISLFTIGVFMAALDNGIISAALTTINESFSVSPSWGSWGITLYTLGLSVSVPIVGKLSDRYGRKKLF
LIEVCLFGLGSLLVALSQSFPLFLISRLIQALGGGGIFIIGSSHILATLPKEKQGKALGLLGAMNGMAAVLGPNIGSFLL
DWTGSWHWLFLINLPIAVLLVVFGACFIAETKAPEAKRLDAAGIFLLSLSILAVMYGMTNLDGANLLHSLGNPEVYGCII
FGILCFAALISYEKRVEMRGGDPILAYSLLRNHMFQRTLIIGLLSGGLLAAVIFIPSYVEQYLGVPAAKAGYWMTPLALA
SGIGAWLGGALTDKKGPVKTVILSGIISCAGFALFPLWVTEKWEFVIASVAAGIGFGFLLGAPLNVLVSEAAKTNKGTAL
GTLSLVRQIGLTLAPTLYAGFITAGFDQIGDEINSRLSDSGYSEKAMQMIPEIDSSEVSSLQEQIERIPVPEVKTAISDA
IHASVASGYDHLYAAAAVVSLLVIAAISIPAFRRQKR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family

Homologues:

Organism=Escherichia coli, GI87081983, Length=435, Percent_Identity=25.0574712643678, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1790195, Length=399, Percent_Identity=26.0651629072682, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1788710, Length=318, Percent_Identity=26.7295597484277, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789042, Length=339, Percent_Identity=27.1386430678466, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1788392, Length=202, Percent_Identity=29.7029702970297, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1790146, Length=145, Percent_Identity=28.9655172413793, Blast_Score=62, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6323735, Length=443, Percent_Identity=24.8306997742664, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6322958, Length=465, Percent_Identity=23.6559139784946, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319770, Length=457, Percent_Identity=23.4135667396061, Blast_Score=63, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YFIU_BACSU (O31563)

Other databases:

- EMBL:   D85082
- EMBL:   AL009126
- PIR:   G69804
- RefSeq:   NP_388721.1
- ProteinModelPortal:   O31563
- SMR:   O31563
- EnsemblBacteria:   EBBACT00000003771
- GeneID:   936182
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU08400
- NMPDR:   fig|224308.1.peg.840
- GenoList:   BSU08400
- GeneTree:   EBGT00050000000114
- HOGENOM:   HBG338648
- OMA:   GPNIGSF
- ProtClustDB:   CLSK886899
- BioCyc:   BSUB:BSU08400-MONOMER
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411
- PRINTS:   PR01036

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 54937; Mature: 54806

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS50850 MFS; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xda19fec)-; HASH(0xdc6f318)-; HASH(0xbe7b87c)-; HASH(0xd43eef4)-; HASH(0xd07b488)-; HASH(0xcaac028)-; HASH(0xdaae870)-; HASH(0xcc16f18)-; HASH(0xdc40284)-; HASH(0xdc68078)-; HASH(0xdc5f4a4)-; HASH(0xd975d84)-; HASH(0xd6b9114)-; HASH(0xd893170)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEALTEQKSQKWAISLFTIGVFMAALDNGIISAALTTINESFSVSPSWGSWGITLYTLG
CCCHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEC
LSVSVPIVGKLSDRYGRKKLFLIEVCLFGLGSLLVALSQSFPLFLISRLIQALGGGGIFI
CEEECCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEE
IGSSHILATLPKEKQGKALGLLGAMNGMAAVLGPNIGSFLLDWTGSWHWLFLINLPIAVL
EECCCEEEECCCCCCCCCEEHHHHHCCHHHHHCCCHHHHHHCCCCCEEEEEEHHHHHHHH
LVVFGACFIAETKAPEAKRLDAAGIFLLSLSILAVMYGMTNLDGANLLHSLGNPEVYGCI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHH
IFGILCFAALISYEKRVEMRGGDPILAYSLLRNHMFQRTLIIGLLSGGLLAAVIFIPSYV
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQYLGVPAAKAGYWMTPLALASGIGAWLGGALTDKKGPVKTVILSGIISCAGFALFPLWV
HHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TEKWEFVIASVAAGIGFGFLLGAPLNVLVSEAAKTNKGTALGTLSLVRQIGLTLAPTLYA
CCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHH
GFITAGFDQIGDEINSRLSDSGYSEKAMQMIPEIDSSEVSSLQEQIERIPVPEVKTAISD
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
AIHASVASGYDHLYAAAAVVSLLVIAAISIPAFRRQKR
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHCHHHHHCCH
>Mature Secondary Structure 
SEALTEQKSQKWAISLFTIGVFMAALDNGIISAALTTINESFSVSPSWGSWGITLYTLG
CCHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEC
LSVSVPIVGKLSDRYGRKKLFLIEVCLFGLGSLLVALSQSFPLFLISRLIQALGGGGIFI
CEEECCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEE
IGSSHILATLPKEKQGKALGLLGAMNGMAAVLGPNIGSFLLDWTGSWHWLFLINLPIAVL
EECCCEEEECCCCCCCCCEEHHHHHCCHHHHHCCCHHHHHHCCCCCEEEEEEHHHHHHHH
LVVFGACFIAETKAPEAKRLDAAGIFLLSLSILAVMYGMTNLDGANLLHSLGNPEVYGCI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHH
IFGILCFAALISYEKRVEMRGGDPILAYSLLRNHMFQRTLIIGLLSGGLLAAVIFIPSYV
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQYLGVPAAKAGYWMTPLALASGIGAWLGGALTDKKGPVKTVILSGIISCAGFALFPLWV
HHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TEKWEFVIASVAAGIGFGFLLGAPLNVLVSEAAKTNKGTALGTLSLVRQIGLTLAPTLYA
CCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHH
GFITAGFDQIGDEINSRLSDSGYSEKAMQMIPEIDSSEVSSLQEQIERIPVPEVKTAISD
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
AIHASVASGYDHLYAAAAVVSLLVIAAISIPAFRRQKR
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHCHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377