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The map label for this gene is yflG

Identifier: 16077836

GI number: 16077836

Start: 839735

End: 840484

Strand: Reverse

Name: yflG

Synonym: BSU07690

Alternate gene names: 16077836

Gene position: 840484-839735 (Counterclockwise)

Preceding gene: 16077838

Following gene: 16077835

Centisome position: 19.94

GC content: 48.13

Gene sequence:

>750_bases
ATGATTGTAACAAACGATCAAGAATTAGAAGGCCTGAAAAAAATCGGAAGAATCGTCGCGCTTGCGCGTGAAGAAATGAA
GCGGAAGGCAGAGCCCGGCATGAGCACAAAAGACCTTGACCTTATCGGAAAAGCTGTGCTTGATGAGCACGGCGCCGTTT
CAGCTCCTGAGAAGGAATACGATTTTCCAGGTGTGACATGTATCAGCGTAAATGATGAAGTGGCTCACGGCATACCAAGC
ACATCCAAAATTTTAAAAGCAGGGGACCTTGTCAACATCGACATCTCCGCTGAATTTGGCGGCTTCTATTCCGACACAGG
CATCTCATTTGTGCTCGGTGAAGGAGAAGAACGCCTTCATAAGCTTTGCCAATGCGCGGAAAACGCTTTTCAAAAGGGGC
TTCAGCAGGCAAAAGCGGGCAAGCGCCAAAACCAGATCGGAAGAGCCGTTTATCATGAAGCACGCTCTCAAGGCTTTACC
GTCATCAAAACCCTGACCGGCCACGGAATCGGCCGAAGCCTGCATGAAGCGCCGAACCACATCATGAACTATTATGATCC
GTTTGATAATGCGCTGTTCAAAAATGGCACAGTCATCGCGCTTGAACCGTTTATTTCAACGAAAGCAGAAACGATTGTTG
AAGCTGGAGACGGCTGGACGTTCAAGACGCCGGATAAAAGCATGGTTGCCCAAGTTGAACACACGATCGTCATCACAAAA
GATGAACCGATTATCCTGACGAAACTGTAA

Upstream 100 bases:

>100_bases
CGGGGGTATTTCTTTTTTCCCAGGTCCGTGTGTTTCCTATTTTTAAGACAGTTGTGGTAAGCTTAATCTAGATTATTTAA
AAAGAAAGCGGGAATGACAG

Downstream 100 bases:

>100_bases
AACACATTCCGGGCTTCCGGCAGGTTTAACCTCCTCCAATTGCAGGTAAAGCATCATTATTCTGTACGAGGAGGTCTTGC
AATGAACAGAGATCAAGAAA

Product: methionine aminopeptidase

Products: NA

Alternate protein names: MAP 2

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MIVTNDQELEGLKKIGRIVALAREEMKRKAEPGMSTKDLDLIGKAVLDEHGAVSAPEKEYDFPGVTCISVNDEVAHGIPS
TSKILKAGDLVNIDISAEFGGFYSDTGISFVLGEGEERLHKLCQCAENAFQKGLQQAKAGKRQNQIGRAVYHEARSQGFT
VIKTLTGHGIGRSLHEAPNHIMNYYDPFDNALFKNGTVIALEPFISTKAETIVEAGDGWTFKTPDKSMVAQVEHTIVITK
DEPIILTKL

Sequences:

>Translated_249_residues
MIVTNDQELEGLKKIGRIVALAREEMKRKAEPGMSTKDLDLIGKAVLDEHGAVSAPEKEYDFPGVTCISVNDEVAHGIPS
TSKILKAGDLVNIDISAEFGGFYSDTGISFVLGEGEERLHKLCQCAENAFQKGLQQAKAGKRQNQIGRAVYHEARSQGFT
VIKTLTGHGIGRSLHEAPNHIMNYYDPFDNALFKNGTVIALEPFISTKAETIVEAGDGWTFKTPDKSMVAQVEHTIVITK
DEPIILTKL
>Mature_249_residues
MIVTNDQELEGLKKIGRIVALAREEMKRKAEPGMSTKDLDLIGKAVLDEHGAVSAPEKEYDFPGVTCISVNDEVAHGIPS
TSKILKAGDLVNIDISAEFGGFYSDTGISFVLGEGEERLHKLCQCAENAFQKGLQQAKAGKRQNQIGRAVYHEARSQGFT
VIKTLTGHGIGRSLHEAPNHIMNYYDPFDNALFKNGTVIALEPFISTKAETIVEAGDGWTFKTPDKSMVAQVEHTIVITK
DEPIILTKL

Specific function: Removes the amino-terminal methionine from nascent proteins

COG id: COG0024

COG function: function code J; Methionine aminopeptidase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24A family

Homologues:

Organism=Homo sapiens, GI164420681, Length=247, Percent_Identity=31.1740890688259, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI40385867, Length=247, Percent_Identity=29.5546558704453, Blast_Score=119, Evalue=2e-27,
Organism=Escherichia coli, GI1786364, Length=246, Percent_Identity=35.7723577235772, Blast_Score=166, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI71996291, Length=247, Percent_Identity=31.9838056680162, Blast_Score=128, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6323273, Length=241, Percent_Identity=31.1203319502075, Blast_Score=122, Evalue=4e-29,
Organism=Drosophila melanogaster, GI21355531, Length=246, Percent_Identity=31.7073170731707, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24583427, Length=241, Percent_Identity=28.6307053941909, Blast_Score=114, Evalue=5e-26,

Paralogues:

None

Copy number: 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): AMPM2_BACSU (O34484)

Other databases:

- EMBL:   D86417
- EMBL:   AL009126
- PIR:   E69810
- RefSeq:   NP_388650.1
- HSSP:   P0A078
- ProteinModelPortal:   O34484
- SMR:   O34484
- MEROPS:   M24.001
- EnsemblBacteria:   EBBACT00000002347
- GeneID:   936121
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU07690
- NMPDR:   fig|224308.1.peg.769
- GenoList:   BSU07690
- GeneTree:   EBGT00070000031874
- HOGENOM:   HBG299384
- OMA:   PGATCIS
- ProtClustDB:   PRK12896
- BioCyc:   BSUB:BSU07690-MONOMER
- GO:   GO:0005737
- GO:   GO:0006508
- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR002467
- Gene3D:   G3DSA:3.90.230.10
- PANTHER:   PTHR10804:SF13
- PANTHER:   PTHR10804
- PRINTS:   PR00599
- TIGRFAMs:   TIGR00500

Pfam domain/function: PF00557 Peptidase_M24; SSF55920 Peptidase_M24_cat_core

EC number: =3.4.11.18

Molecular weight: Translated: 27211; Mature: 27211

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: BINDING 76-76 BINDING 175-175

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVTNDQELEGLKKIGRIVALAREEMKRKAEPGMSTKDLDLIGKAVLDEHGAVSAPEKEY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
DFPGVTCISVNDEVAHGIPSTSKILKAGDLVNIDISAEFGGFYSDTGISFVLGEGEERLH
CCCCEEEEECCHHHHHCCCCHHHHHHCCCEEEEEEECCCCCEECCCCCEEEECCCHHHHH
KLCQCAENAFQKGLQQAKAGKRQNQIGRAVYHEARSQGFTVIKTLTGHGIGRSLHEAPNH
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEHHCCCCCHHHHHHHHH
IMNYYDPFDNALFKNGTVIALEPFISTKAETIVEAGDGWTFKTPDKSMVAQVEHTIVITK
HHHHCCCHHHHHHCCCCEEEECCCCCCHHHHHHCCCCCCEEECCCHHHHHHHEEEEEEEC
DEPIILTKL
CCCEEEEEC
>Mature Secondary Structure
MIVTNDQELEGLKKIGRIVALAREEMKRKAEPGMSTKDLDLIGKAVLDEHGAVSAPEKEY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
DFPGVTCISVNDEVAHGIPSTSKILKAGDLVNIDISAEFGGFYSDTGISFVLGEGEERLH
CCCCEEEEECCHHHHHCCCCHHHHHHCCCEEEEEEECCCCCEECCCCCEEEECCCHHHHH
KLCQCAENAFQKGLQQAKAGKRQNQIGRAVYHEARSQGFTVIKTLTGHGIGRSLHEAPNH
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEHHCCCCCHHHHHHHHH
IMNYYDPFDNALFKNGTVIALEPFISTKAETIVEAGDGWTFKTPDKSMVAQVEHTIVITK
HHHHCCCHHHHHHCCCCEEEECCCCCCHHHHHHCCCCCCEEECCCHHHHHHHEEEEEEEC
DEPIILTKL
CCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377