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The map label for this gene is yflE

Identifier: 16077838

GI number: 16077838

Start: 842047

End: 843996

Strand: Reverse

Name: yflE

Synonym: BSU07710

Alternate gene names: 16077838

Gene position: 843996-842047 (Counterclockwise)

Preceding gene: 16077843

Following gene: 16077836

Centisome position: 20.02

GC content: 42.1

Gene sequence:

>1950_bases
ATGAAAACATTTATAAAAGAAAGAGGACTGGCCTTCTTCTTAATTGCGGTCGTCCTGTTATGGATCAAAACGTATGTCGG
TTATGTCCTGAATTTCAACTTAGGAATAGACAACACGATACAAAAAATATTGCTTTTTGTGAATCCTCTTAGCTCAAGCT
TGTTCTTTCTTGGCTTTGGACTCTTGTTCAAGAAAAAATTACAGCAGACAGCCATTATAGTGATTCATTTTTTAATGTCT
TTTTTACTGTACGCCAACATTGTGTACTACAGATTTTTCAATGATTTTATTACAATTCCGGTCATTATGCAGGCTAAAAC
AAACGGCGGCCAACTCGGTGACAGCGCATTTTCGCTGATGAGACCGACTGACGCCTTTTACTTTATCGATACGATCATCC
TGATCATCTTGGCGATCAAAGTAAACAAGCCTGCCGAAACGTCAAGCAAAAAATCGTTCCGAATTATTTTTGCGTCTTCA
ATTCTTGTGTTCTTGATCAACCTGGCAGTTGCGGAATCAGACCGTCCTGAATTGCTGACAAGATCATTCGACCGGAACTA
TCTTGTGAAATACTTGGGAACATACAATTTCACGATTTATGACGCTGTACAGAATATCAAGTCCAACAGCCAGCGCGCGC
TTGCCGATTCCAGCGACGTAACGGAAGTAGAAAACTACATGAAAGCCAATTACGATGTGCCGAATAACGTGTATTTCGGC
AAAGCGGAAGGAAAAAACGTCATTTACGTTTCACTTGAATCTTTGCAGTCATTTATCATCGACTATAAAATTGACGGCAA
AGAAGTGACACCATTCTTAAATAAACTGGCACATGATAACGAAACGTTCTACTTTGATAACTTTTTCCACCAAACGGGAC
AAGGTAAAACATCTGATGCTGAATTTATGATGGAAAACTCTCTGTACCCGCTGGCTCAAGGTTCAGTTTTCGTAAACAAA
GCGCAAAACACGCTGCAATCCGTTCCGGCGATTCTGAAGTCTAAGAATTACACATCTGCTACTTTCCACGGGAACACGCA
GACGTTCTGGAACCGTAACGAAATGTACAAGGCGGAAGGCATTGATAAATTCTTTGATTCTGCTTACTATGACATGAACG
AAGAAAACACGAAAAACTACGGCATGAAAGACAAACCGTTCTTCAAAGAATCAATGCCGCTGCTGGAAAGCCTGCCGCAG
CCGTTCTATACGAAGTTCATTACCCTTTCCAACCACTTCCCATTCGGAATGGATGAGGGGGATACAGACTTCCCGGCTGG
AGACTTTGGTGACTCTGTCGTCGATAACTATTTCCAGTCAGCCCATTACCTTGATCAGTCCATTGAACAATTCTTCAATG
ATCTGAAAAAAGACGGGTTATATGATAAATCGATTATTGTGATGTACGGAGACCACTACGGCATCTCTGAAAACCACAAT
AAAGCGATGGCGAAAGTGCTTGGCAAGGATGAAATCACTGATTACGACAACGCCCAGCTTCAACGGGTGCCGCTCTTTAT
CCACGCTGCCGGCGTGAAGGGCGAGAAAGTTCATAAATATGCCGGAGACGTTGATGTGGCTCCTACCATTCTGCATCTGC
TCGGAGTGGATACGAAGGACTATCTGATGTCCGGTTCTGATATTTTATCGAAAGAACACCGTGAAGTGATTCCGTTCCGA
AACGGAGACTTTATTTCACCGAAGTACACGAAAATATCCGGTAAGTATTACGACACGAAAACCGGAAAAGAACTCGATGA
ATCCGAAGTCGACAAGTCAGAAGACTCACTCGTCAAGAAGGAACTTGAAATGTCCGATAAAATCATAAACGGAGACCTGC
TGCGGTTCTACGAGCCGAAAGGTTTTAAGAAGGTGAATCCTTCTGATTATGATTACACAAAACATGACGAAGATTCTTCC
GAAACGTCAAAGGATAACGAAGATAAATAA

Upstream 100 bases:

>100_bases
ACGATTTTCACTTTTCTAATCTGCTGATTCGTGTTATATTGGATACGTTCGTTTTTTCTATCGTTTCGCTCGAACTGGAT
CGGAAAAAAGGAGTGTAACA

Downstream 100 bases:

>100_bases
GAAAAAGCGGAGAGGTTGCCCTCTCCGCTTTTTTATTTGACAGCAGCCCTCAATTCCTCAGCTGCAAATTCCACATTCGG
GCCAATAATGACTTGAACCG

Product: exported enzyme and anion transporter

Products: NA

Alternate protein names: LTA synthase 2; Polyglycerol phosphate synthase 2

Number of amino acids: Translated: 649; Mature: 649

Protein sequence:

>649_residues
MKTFIKERGLAFFLIAVVLLWIKTYVGYVLNFNLGIDNTIQKILLFVNPLSSSLFFLGFGLLFKKKLQQTAIIVIHFLMS
FLLYANIVYYRFFNDFITIPVIMQAKTNGGQLGDSAFSLMRPTDAFYFIDTIILIILAIKVNKPAETSSKKSFRIIFASS
ILVFLINLAVAESDRPELLTRSFDRNYLVKYLGTYNFTIYDAVQNIKSNSQRALADSSDVTEVENYMKANYDVPNNVYFG
KAEGKNVIYVSLESLQSFIIDYKIDGKEVTPFLNKLAHDNETFYFDNFFHQTGQGKTSDAEFMMENSLYPLAQGSVFVNK
AQNTLQSVPAILKSKNYTSATFHGNTQTFWNRNEMYKAEGIDKFFDSAYYDMNEENTKNYGMKDKPFFKESMPLLESLPQ
PFYTKFITLSNHFPFGMDEGDTDFPAGDFGDSVVDNYFQSAHYLDQSIEQFFNDLKKDGLYDKSIIVMYGDHYGISENHN
KAMAKVLGKDEITDYDNAQLQRVPLFIHAAGVKGEKVHKYAGDVDVAPTILHLLGVDTKDYLMSGSDILSKEHREVIPFR
NGDFISPKYTKISGKYYDTKTGKELDESEVDKSEDSLVKKELEMSDKIINGDLLRFYEPKGFKKVNPSDYDYTKHDEDSS
ETSKDNEDK

Sequences:

>Translated_649_residues
MKTFIKERGLAFFLIAVVLLWIKTYVGYVLNFNLGIDNTIQKILLFVNPLSSSLFFLGFGLLFKKKLQQTAIIVIHFLMS
FLLYANIVYYRFFNDFITIPVIMQAKTNGGQLGDSAFSLMRPTDAFYFIDTIILIILAIKVNKPAETSSKKSFRIIFASS
ILVFLINLAVAESDRPELLTRSFDRNYLVKYLGTYNFTIYDAVQNIKSNSQRALADSSDVTEVENYMKANYDVPNNVYFG
KAEGKNVIYVSLESLQSFIIDYKIDGKEVTPFLNKLAHDNETFYFDNFFHQTGQGKTSDAEFMMENSLYPLAQGSVFVNK
AQNTLQSVPAILKSKNYTSATFHGNTQTFWNRNEMYKAEGIDKFFDSAYYDMNEENTKNYGMKDKPFFKESMPLLESLPQ
PFYTKFITLSNHFPFGMDEGDTDFPAGDFGDSVVDNYFQSAHYLDQSIEQFFNDLKKDGLYDKSIIVMYGDHYGISENHN
KAMAKVLGKDEITDYDNAQLQRVPLFIHAAGVKGEKVHKYAGDVDVAPTILHLLGVDTKDYLMSGSDILSKEHREVIPFR
NGDFISPKYTKISGKYYDTKTGKELDESEVDKSEDSLVKKELEMSDKIINGDLLRFYEPKGFKKVNPSDYDYTKHDEDSS
ETSKDNEDK
>Mature_649_residues
MKTFIKERGLAFFLIAVVLLWIKTYVGYVLNFNLGIDNTIQKILLFVNPLSSSLFFLGFGLLFKKKLQQTAIIVIHFLMS
FLLYANIVYYRFFNDFITIPVIMQAKTNGGQLGDSAFSLMRPTDAFYFIDTIILIILAIKVNKPAETSSKKSFRIIFASS
ILVFLINLAVAESDRPELLTRSFDRNYLVKYLGTYNFTIYDAVQNIKSNSQRALADSSDVTEVENYMKANYDVPNNVYFG
KAEGKNVIYVSLESLQSFIIDYKIDGKEVTPFLNKLAHDNETFYFDNFFHQTGQGKTSDAEFMMENSLYPLAQGSVFVNK
AQNTLQSVPAILKSKNYTSATFHGNTQTFWNRNEMYKAEGIDKFFDSAYYDMNEENTKNYGMKDKPFFKESMPLLESLPQ
PFYTKFITLSNHFPFGMDEGDTDFPAGDFGDSVVDNYFQSAHYLDQSIEQFFNDLKKDGLYDKSIIVMYGDHYGISENHN
KAMAKVLGKDEITDYDNAQLQRVPLFIHAAGVKGEKVHKYAGDVDVAPTILHLLGVDTKDYLMSGSDILSKEHREVIPFR
NGDFISPKYTKISGKYYDTKTGKELDESEVDKSEDSLVKKELEMSDKIINGDLLRFYEPKGFKKVNPSDYDYTKHDEDSS
ETSKDNEDK

Specific function: Catalyzes the polymerization of lipoteichoic acid (LTA) polyglycerol phosphate, a reaction that presumably uses phosphatidylglycerol (PG) as substrate

COG id: COG1368

COG function: function code M; Phosphoglycerol transferase and related proteins, alkaline phosphatase superfamily

Gene ontology:

Cell location: Processed glycerol phosphate lipoteichoic acid synthase 2:Secreted. Note=The secretion into the extracellular medium is dependent on secA and ffh

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LTA synthase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LTAS2_BACSU (O34952)

Other databases:

- EMBL:   D86417
- EMBL:   AL009126
- PIR:   C69810
- RefSeq:   NP_388652.1
- PDB:   2W8D
- PDBsum:   2W8D
- ProteinModelPortal:   O34952
- SMR:   O34952
- EnsemblBacteria:   EBBACT00000000671
- GeneID:   939200
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU07710
- NMPDR:   fig|224308.1.peg.771
- GenoList:   BSU07710
- GeneTree:   EBGT00050000000986
- HOGENOM:   HBG425954
- OMA:   FFNDFIT
- ProtClustDB:   CLSK886866
- BioCyc:   BSUB:BSU07710-MONOMER
- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012160
- InterPro:   IPR000917
- Gene3D:   G3DSA:3.40.720.10
- PIRSF:   PIRSF005091

Pfam domain/function: PF00884 Sulfatase; SSF53649 Alkaline_phosphatase_core

EC number: 3.1.6.- [C]

Molecular weight: Translated: 74235; Mature: 74235

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: ACT_SITE 297-297 BINDING 412-412

Signals:

None

Transmembrane regions:

HASH(0xc07fd90)-; HASH(0xd423a7c)-; HASH(0xd9eb608)-; HASH(0xd2b735c)-; HASH(0xdaa1000)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTFIKERGLAFFLIAVVLLWIKTYVGYVLNFNLGIDNTIQKILLFVNPLSSSLFFLGFG
CCCCHHHCCHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHH
LLFKKKLQQTAIIVIHFLMSFLLYANIVYYRFFNDFITIPVIMQAKTNGGQLGDSAFSLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCHHHHHHH
RPTDAFYFIDTIILIILAIKVNKPAETSSKKSFRIIFASSILVFLINLAVAESDRPELLT
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCHHHHH
RSFDRNYLVKYLGTYNFTIYDAVQNIKSNSQRALADSSDVTEVENYMKANYDVPNNVYFG
HHCCHHHHHHHHCCCCEEHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHCCCCCCCCEEEE
KAEGKNVIYVSLESLQSFIIDYKIDGKEVTPFLNKLAHDNETFYFDNFFHQTGQGKTSDA
CCCCCEEEEEEHHHHHHHHEEEEECCCHHHHHHHHHCCCCCEEEEEHHHHHCCCCCCCCH
EFMMENSLYPLAQGSVFVNKAQNTLQSVPAILKSKNYTSATFHGNTQTFWNRNEMYKAEG
HHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHEHHCC
IDKFFDSAYYDMNEENTKNYGMKDKPFFKESMPLLESLPQPFYTKFITLSNHFPFGMDEG
HHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCHHHHHCCCCHHHEEEEECCCCCCCCCCC
DTDFPAGDFGDSVVDNYFQSAHYLDQSIEQFFNDLKKDGLYDKSIIVMYGDHYGISENHN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCH
KAMAKVLGKDEITDYDNAQLQRVPLFIHAAGVKGEKVHKYAGDVDVAPTILHLLGVDTKD
HHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHCCCCCHHHHHHHHHCCCHHH
YLMSGSDILSKEHREVIPFRNGDFISPKYTKISGKYYDTKTGKELDESEVDKSEDSLVKK
HHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCEEECCCCCCCCCHHHCCCCHHHHHHH
ELEMSDKIINGDLLRFYEPKGFKKVNPSDYDYTKHDEDSSETSKDNEDK
HHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
>Mature Secondary Structure
MKTFIKERGLAFFLIAVVLLWIKTYVGYVLNFNLGIDNTIQKILLFVNPLSSSLFFLGFG
CCCCHHHCCHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHH
LLFKKKLQQTAIIVIHFLMSFLLYANIVYYRFFNDFITIPVIMQAKTNGGQLGDSAFSLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCHHHHHHH
RPTDAFYFIDTIILIILAIKVNKPAETSSKKSFRIIFASSILVFLINLAVAESDRPELLT
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCHHHHH
RSFDRNYLVKYLGTYNFTIYDAVQNIKSNSQRALADSSDVTEVENYMKANYDVPNNVYFG
HHCCHHHHHHHHCCCCEEHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHCCCCCCCCEEEE
KAEGKNVIYVSLESLQSFIIDYKIDGKEVTPFLNKLAHDNETFYFDNFFHQTGQGKTSDA
CCCCCEEEEEEHHHHHHHHEEEEECCCHHHHHHHHHCCCCCEEEEEHHHHHCCCCCCCCH
EFMMENSLYPLAQGSVFVNKAQNTLQSVPAILKSKNYTSATFHGNTQTFWNRNEMYKAEG
HHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHEHHCC
IDKFFDSAYYDMNEENTKNYGMKDKPFFKESMPLLESLPQPFYTKFITLSNHFPFGMDEG
HHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCHHHHHCCCCHHHEEEEECCCCCCCCCCC
DTDFPAGDFGDSVVDNYFQSAHYLDQSIEQFFNDLKKDGLYDKSIIVMYGDHYGISENHN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCH
KAMAKVLGKDEITDYDNAQLQRVPLFIHAAGVKGEKVHKYAGDVDVAPTILHLLGVDTKD
HHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCCHHHHHCCCCCHHHHHHHHHCCCHHH
YLMSGSDILSKEHREVIPFRNGDFISPKYTKISGKYYDTKTGKELDESEVDKSEDSLVKK
HHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCEEECCCCCCCCCHHHCCCCHHHHHHH
ELEMSDKIINGDLLRFYEPKGFKKVNPSDYDYTKHDEDSSETSKDNEDK
HHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377; 10658653