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The map label for this gene is citT

Identifier: 16077826

GI number: 16077826

Start: 832545

End: 833225

Strand: Direct

Name: citT

Synonym: BSU07590

Alternate gene names: 16077826

Gene position: 832545-833225 (Clockwise)

Preceding gene: 16077825

Following gene: 255767186

Centisome position: 19.75

GC content: 48.02

Gene sequence:

>681_bases
TTGATTCACATCGCGATTGCGGAGGATGATTTTCGAGTTGCGCAAATCCATGAGAGATTGATTAAACAGCTTGATGGATT
CAAGATTATCGGCAAGGCGGCTAACGCAAAAGAGACATTGGCGCTTTTGAAGGAACACAAGGCTGATTTGCTTCTGCTGG
ATATTTATATGCCGGACGAGCTTGGGACCGCGTTGATTCCTGATATACGAAGCCGATTTCCTGAAGTGGACATTATGATT
ATCACAGCGGCAACAGAAACCCGTCATTTGCAGGAAGCGCTAAGGGCGGGAATTGCCCACTATTTGATCAAACCCGTAAC
AGCTGACAAGTTCAGGCAGGTGCTGCTTCAGTATAAAGAAAAAAGGAAGCTGCTCATGTCTCAGCCGGAGGTCAGCCAAT
CCATGATCGACCATATTTTTGGGAACGGTGTGAAGACAGCTTTGCCGGCAGAGGATTTGCCGACTGGCATTAATTCGATT
ACACTGCGAAAAATTAAGGAAGCGCTTCAGACTGCGTCAGAAGGATTGACAGCGGAGGAACTTGGGGAAAAAATGGGGGC
GTCACGAACAACTGCCCGCCGTTATGCCGAGTACCTTGTGTCAAAGGAAGAAGCAAGAGCCGAGCTTGAATACGGGATTA
TCGGCAGGCCGGAGAGAAAATATTATTTGGCGGCGGATTAG

Upstream 100 bases:

>100_bases
GTGCGTGAACTGGGCGGCTGGATCGAACTGGCGAATCAAAAAACTGGCGGGGCGGTATTCACTGTATTTATACCGAAGGA
GAAACAAAGGGGGAATCCAT

Downstream 100 bases:

>100_bases
ATATGAAAAAATCTATAATCCTATTGAATATTCTATTGATCTTTATGCAGGGTGATATCAGGCAGGCGGCTGCGCCGCGC
CTGCCGGACGGGCCGATAGA

Product: two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MIHIAIAEDDFRVAQIHERLIKQLDGFKIIGKAANAKETLALLKEHKADLLLLDIYMPDELGTALIPDIRSRFPEVDIMI
ITAATETRHLQEALRAGIAHYLIKPVTADKFRQVLLQYKEKRKLLMSQPEVSQSMIDHIFGNGVKTALPAEDLPTGINSI
TLRKIKEALQTASEGLTAEELGEKMGASRTTARRYAEYLVSKEEARAELEYGIIGRPERKYYLAAD

Sequences:

>Translated_226_residues
MIHIAIAEDDFRVAQIHERLIKQLDGFKIIGKAANAKETLALLKEHKADLLLLDIYMPDELGTALIPDIRSRFPEVDIMI
ITAATETRHLQEALRAGIAHYLIKPVTADKFRQVLLQYKEKRKLLMSQPEVSQSMIDHIFGNGVKTALPAEDLPTGINSI
TLRKIKEALQTASEGLTAEELGEKMGASRTTARRYAEYLVSKEEARAELEYGIIGRPERKYYLAAD
>Mature_226_residues
MIHIAIAEDDFRVAQIHERLIKQLDGFKIIGKAANAKETLALLKEHKADLLLLDIYMPDELGTALIPDIRSRFPEVDIMI
ITAATETRHLQEALRAGIAHYLIKPVTADKFRQVLLQYKEKRKLLMSQPEVSQSMIDHIFGNGVKTALPAEDLPTGINSI
TLRKIKEALQTASEGLTAEELGEKMGASRTTARRYAEYLVSKEEARAELEYGIIGRPERKYYLAAD

Specific function: Member of the two-component regulatory system CitT/CitS. Regulates the expression of the citM-yflN operon. Phosphorylated CitT binds to the citM promoter to activate the transcription of the citM-yflN operon

COG id: COG4565

COG function: function code KT; Response regulator of citrate/malate metabolism

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1786838, Length=218, Percent_Identity=34.4036697247706, Blast_Score=139, Evalue=1e-34,
Organism=Escherichia coli, GI1790566, Length=230, Percent_Identity=28.695652173913, Blast_Score=118, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CITT_BACSU (O34534)

Other databases:

- EMBL:   D86417
- EMBL:   AL009126
- PIR:   F69600
- RefSeq:   NP_388640.1
- ProteinModelPortal:   O34534
- EnsemblBacteria:   EBBACT00000001665
- GeneID:   938806
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU07590
- NMPDR:   fig|224308.1.peg.759
- GenoList:   BSU07590
- GeneTree:   EBGT00050000000045
- HOGENOM:   HBG753323
- OMA:   LHSNNEV
- PhylomeDB:   O34534
- ProtClustDB:   CLSK886856
- BioCyc:   BSUB:BSU07590-MONOMER
- GO:   GO:0005737
- GO:   GO:0006350
- InterPro:   IPR011006
- InterPro:   IPR013196
- InterPro:   IPR001789
- SMART:   SM00448

Pfam domain/function: PF08279 HTH_11; PF00072 Response_reg; SSF52172 CheY_like

EC number: NA

Molecular weight: Translated: 25411; Mature: 25411

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHIAIAEDDFRVAQIHERLIKQLDGFKIIGKAANAKETLALLKEHKADLLLLDIYMPDE
CEEEEEECCCHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCH
LGTALIPDIRSRFPEVDIMIITAATETRHLQEALRAGIAHYLIKPVTADKFRQVLLQYKE
HCHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KRKLLMSQPEVSQSMIDHIFGNGVKTALPAEDLPTGINSITLRKIKEALQTASEGLTAEE
HHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHCCCCHHH
LGEKMGASRTTARRYAEYLVSKEEARAELEYGIIGRPERKYYLAAD
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECC
>Mature Secondary Structure
MIHIAIAEDDFRVAQIHERLIKQLDGFKIIGKAANAKETLALLKEHKADLLLLDIYMPDE
CEEEEEECCCHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCH
LGTALIPDIRSRFPEVDIMIITAATETRHLQEALRAGIAHYLIKPVTADKFRQVLLQYKE
HCHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KRKLLMSQPEVSQSMIDHIFGNGVKTALPAEDLPTGINSITLRKIKEALQTASEGLTAEE
HHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHCCCCHHH
LGEKMGASRTTARRYAEYLVSKEEARAELEYGIIGRPERKYYLAAD
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377; 10972810