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The map label for this gene is citS

Identifier: 16077825

GI number: 16077825

Start: 830945

End: 832573

Strand: Direct

Name: citS

Synonym: BSU07580

Alternate gene names: 16077825

Gene position: 830945-832573 (Clockwise)

Preceding gene: 16077824

Following gene: 16077826

Centisome position: 19.71

GC content: 47.58

Gene sequence:

>1629_bases
GTGGTGAAAAAGCGGTTCCATTTTTCCCTGCAAACAAAAATAATGGGGCTGATTGCGGCTCTGCTGGTCTTTGTCATTGG
TGTGCTGACCATTACGTTAGCCGTTCAGCATACACAGGGAGAACGGAGACAGGCAGAGCAGCTGGCGGTTCAAACGGCGA
GAACCATTTCCTATATGCCGCCGGTTAAAGAGCTCATTGAGAGAAAAGACGGACATGCGGCTCAGACGCAAGAGGTCATT
GAACAAATGAAAGAACAGACTGGTGCGTTTGCCATTTATGTTTTGAACGAAAAAGGAGACATTCGCAGCGCCTCTGGAAA
AAGCGGATTAAAGAAACTGGAGCGCAGCAGAGAAATTTTGTTTGGCGGTTCGCATGTTTCTGAAACAAAAGCGGATGGAC
GAAGAGTGATCAGAGGGAGCGCGCCGATTATAAAAGAACAGAAGGGATACAGCCAAGTGATCGGCAGCGTGTCTGTTGAT
TTTCTGCAAACGGAGACAGAGCAAAGCATCAAAAAGCATTTGAGAAATTTGAGTGTGATTGCTGTGCTTGTACTGCTGCT
CGGATTTATTGGCGCCGCCGTGCTGGCGAAAAGCATCAGAAAGGATACGCTCGGGCTTGAACCGCATGAGATCGCGGCTC
TATATCGTGAGAGGAACGCAATGCTTTTCGCGATTCGAGAAGGGATTATTGCCACCAATCGTGAAGGCGTCGTCACCATG
ATGAACGTATCGGCGGCCGAGATGCTGAAGCTGCCCGAGCCTGTGATCCATCTTCCTATAGATGACGTCATGCCGGGAGC
AGGGCTGATGTCTGTGCTTGAAAAAGGAGAAATGCTGCCGAACCAGGAAGTAAGCGTCAACGATCAAGTGTTTATTATCA
ATACGAAAGTGATGAATCAAGGCGGGCAGGCGTATGGGATTGTCGTCAGCTTCAGGGAGAAAACAGAGCTGAAGAAGCTG
ATCGACACATTGACAGAGGTTCGCAAATATTCAGAGGATCTCAGGGCGCAGACTCATGAATTTTCAAATAAGCTTTATGC
GATTTTAGGGCTGCTTGAGCTTGGGGAGTATGATGAAGCCATTGATCTGATTAAAGAGGAATATGCGATACAAAATGAAC
AGCATGATCTTTTATTCCATAACATCCATTCGCAGCAGGTGCAGGCCATTTTGTTAGGGAAAATAAGCAAGGCATCGGAA
AAGAAGGTCAAGCTGGTGATTGATGAGAACAGCTCACTCGCGCCTCTTCCTGCGCATATCGGCTTGTCCCATCTTATTAC
GATTATTGGCAATTTAATTGATAACGCTTTCGAAGCTGTAGCGGAGCAAAGCGTGAAGGAAGTTTTGTTTTTTATCACGG
ATATGGGCCATGACATTGTCATTGAAGTATCAGACACAGGGCCCGGTGTGCCGCCAGAGAAAATAGAAGCTGTGTTTGAG
AGAGGCTATTCTTCAAAGGGGATGAGGAGAGGCTACGGTCTGGCCAATGTGAAAGACTCAGTGCGTGAACTGGGCGGCTG
GATCGAACTGGCGAATCAAAAAACTGGCGGGGCGGTATTCACTGTATTTATACCGAAGGAGAAACAAAGGGGGAATCCAT
TTGATTCACATCGCGATTGCGGAGGATGA

Upstream 100 bases:

>100_bases
TTTTTTTATTTTCACTCCTTCGTAAGAAAATGGATTTTGAAAAATGAGAAAATTCCCTGTGAAAAATGGTATGATCTAGG
TAGAAAGGACGGCTGGTGCT

Downstream 100 bases:

>100_bases
TTTTCGAGTTGCGCAAATCCATGAGAGATTGATTAAACAGCTTGATGGATTCAAGATTATCGGCAAGGCGGCTAACGCAA
AAGAGACATTGGCGCTTTTG

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 542; Mature: 542

Protein sequence:

>542_residues
MVKKRFHFSLQTKIMGLIAALLVFVIGVLTITLAVQHTQGERRQAEQLAVQTARTISYMPPVKELIERKDGHAAQTQEVI
EQMKEQTGAFAIYVLNEKGDIRSASGKSGLKKLERSREILFGGSHVSETKADGRRVIRGSAPIIKEQKGYSQVIGSVSVD
FLQTETEQSIKKHLRNLSVIAVLVLLLGFIGAAVLAKSIRKDTLGLEPHEIAALYRERNAMLFAIREGIIATNREGVVTM
MNVSAAEMLKLPEPVIHLPIDDVMPGAGLMSVLEKGEMLPNQEVSVNDQVFIINTKVMNQGGQAYGIVVSFREKTELKKL
IDTLTEVRKYSEDLRAQTHEFSNKLYAILGLLELGEYDEAIDLIKEEYAIQNEQHDLLFHNIHSQQVQAILLGKISKASE
KKVKLVIDENSSLAPLPAHIGLSHLITIIGNLIDNAFEAVAEQSVKEVLFFITDMGHDIVIEVSDTGPGVPPEKIEAVFE
RGYSSKGMRRGYGLANVKDSVRELGGWIELANQKTGGAVFTVFIPKEKQRGNPFDSHRDCGG

Sequences:

>Translated_542_residues
MVKKRFHFSLQTKIMGLIAALLVFVIGVLTITLAVQHTQGERRQAEQLAVQTARTISYMPPVKELIERKDGHAAQTQEVI
EQMKEQTGAFAIYVLNEKGDIRSASGKSGLKKLERSREILFGGSHVSETKADGRRVIRGSAPIIKEQKGYSQVIGSVSVD
FLQTETEQSIKKHLRNLSVIAVLVLLLGFIGAAVLAKSIRKDTLGLEPHEIAALYRERNAMLFAIREGIIATNREGVVTM
MNVSAAEMLKLPEPVIHLPIDDVMPGAGLMSVLEKGEMLPNQEVSVNDQVFIINTKVMNQGGQAYGIVVSFREKTELKKL
IDTLTEVRKYSEDLRAQTHEFSNKLYAILGLLELGEYDEAIDLIKEEYAIQNEQHDLLFHNIHSQQVQAILLGKISKASE
KKVKLVIDENSSLAPLPAHIGLSHLITIIGNLIDNAFEAVAEQSVKEVLFFITDMGHDIVIEVSDTGPGVPPEKIEAVFE
RGYSSKGMRRGYGLANVKDSVRELGGWIELANQKTGGAVFTVFIPKEKQRGNPFDSHRDCGG
>Mature_542_residues
MVKKRFHFSLQTKIMGLIAALLVFVIGVLTITLAVQHTQGERRQAEQLAVQTARTISYMPPVKELIERKDGHAAQTQEVI
EQMKEQTGAFAIYVLNEKGDIRSASGKSGLKKLERSREILFGGSHVSETKADGRRVIRGSAPIIKEQKGYSQVIGSVSVD
FLQTETEQSIKKHLRNLSVIAVLVLLLGFIGAAVLAKSIRKDTLGLEPHEIAALYRERNAMLFAIREGIIATNREGVVTM
MNVSAAEMLKLPEPVIHLPIDDVMPGAGLMSVLEKGEMLPNQEVSVNDQVFIINTKVMNQGGQAYGIVVSFREKTELKKL
IDTLTEVRKYSEDLRAQTHEFSNKLYAILGLLELGEYDEAIDLIKEEYAIQNEQHDLLFHNIHSQQVQAILLGKISKASE
KKVKLVIDENSSLAPLPAHIGLSHLITIIGNLIDNAFEAVAEQSVKEVLFFITDMGHDIVIEVSDTGPGVPPEKIEAVFE
RGYSSKGMRRGYGLANVKDSVRELGGWIELANQKTGGAVFTVFIPKEKQRGNPFDSHRDCGG

Specific function: Member of the two-component regulatory system CitT/CitS. Regulates the expression of the citM-yflN operon. Functions probably as a membrane-associated protein kinase that phosphorylates CitT in response to environmental citrate or Mg(2+)-citrate complex

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain

Homologues:

Organism=Escherichia coli, GI1790567, Length=539, Percent_Identity=28.7569573283859, Blast_Score=213, Evalue=4e-56,
Organism=Escherichia coli, GI1786837, Length=553, Percent_Identity=29.2947558770344, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1790436, Length=136, Percent_Identity=28.6764705882353, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CITS_BACSU (O34427)

Other databases:

- EMBL:   D86417
- EMBL:   AL009126
- PIR:   E69600
- RefSeq:   NP_388639.1
- ProteinModelPortal:   O34427
- EnsemblBacteria:   EBBACT00000001891
- GeneID:   939687
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU07580
- NMPDR:   fig|224308.1.peg.758
- GenoList:   BSU07580
- GeneTree:   EBGT00050000000017
- HOGENOM:   HBG537380
- OMA:   ELGGWIE
- PhylomeDB:   O34427
- ProtClustDB:   CLSK886855
- BioCyc:   BSUB:BSU07580-MONOMER
- BRENDA:   2.7.13.3
- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121
- Gene3D:   G3DSA:3.30.565.10
- Gene3D:   G3DSA:1.10.287.130
- PRINTS:   PR00344
- SMART:   SM00387
- SMART:   SM00091

Pfam domain/function: PF02518 HATPase_c; SSF55874 ATP_bd_ATPase; SSF55890 Sensor_kinase_SpoOB-type

EC number: =2.7.13.3

Molecular weight: Translated: 59892; Mature: 59892

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS50109 HIS_KIN; PS50112 PAS; PS00697 DNA_LIGASE_A1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xcc7fbbc)-; HASH(0xd88c63c)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKRFHFSLQTKIMGLIAALLVFVIGVLTITLAVQHTQGERRQAEQLAVQTARTISYMP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
PVKELIERKDGHAAQTQEVIEQMKEQTGAFAIYVLNEKGDIRSASGKSGLKKLERSREIL
CHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCEE
FGGSHVSETKADGRRVIRGSAPIIKEQKGYSQVIGSVSVDFLQTETEQSIKKHLRNLSVI
ECCCCCCHHHHCCCEEEECCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVLVLLLGFIGAAVLAKSIRKDTLGLEPHEIAALYRERNAMLFAIREGIIATNREGVVTM
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCEECCCCCEEEE
MNVSAAEMLKLPEPVIHLPIDDVMPGAGLMSVLEKGEMLPNQEVSVNDQVFIINTKVMNQ
ECCCHHHHHHCCCCEEECCHHHCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEHHHCC
GGQAYGIVVSFREKTELKKLIDTLTEVRKYSEDLRAQTHEFSNKLYAILGLLELGEYDEA
CCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
IDLIKEEYAIQNEQHDLLFHNIHSQQVQAILLGKISKASEKKVKLVIDENSSLAPLPAHI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHH
GLSHLITIIGNLIDNAFEAVAEQSVKEVLFFITDMGHDIVIEVSDTGPGVPPEKIEAVFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHH
RGYSSKGMRRGYGLANVKDSVRELGGWIELANQKTGGAVFTVFIPKEKQRGNPFDSHRDC
CCCCCCCCCCCCCCCHHHHHHHHHHHHHEECCCCCCCEEEEEEECCCCCCCCCCCCCCCC
GG
CH
>Mature Secondary Structure
MVKKRFHFSLQTKIMGLIAALLVFVIGVLTITLAVQHTQGERRQAEQLAVQTARTISYMP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
PVKELIERKDGHAAQTQEVIEQMKEQTGAFAIYVLNEKGDIRSASGKSGLKKLERSREIL
CHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCCEE
FGGSHVSETKADGRRVIRGSAPIIKEQKGYSQVIGSVSVDFLQTETEQSIKKHLRNLSVI
ECCCCCCHHHHCCCEEEECCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVLVLLLGFIGAAVLAKSIRKDTLGLEPHEIAALYRERNAMLFAIREGIIATNREGVVTM
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCEECCCCCEEEE
MNVSAAEMLKLPEPVIHLPIDDVMPGAGLMSVLEKGEMLPNQEVSVNDQVFIINTKVMNQ
ECCCHHHHHHCCCCEEECCHHHCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEHHHCC
GGQAYGIVVSFREKTELKKLIDTLTEVRKYSEDLRAQTHEFSNKLYAILGLLELGEYDEA
CCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
IDLIKEEYAIQNEQHDLLFHNIHSQQVQAILLGKISKASEKKVKLVIDENSSLAPLPAHI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHH
GLSHLITIIGNLIDNAFEAVAEQSVKEVLFFITDMGHDIVIEVSDTGPGVPPEKIEAVFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHH
RGYSSKGMRRGYGLANVKDSVRELGGWIELANQKTGGAVFTVFIPKEKQRGNPFDSHRDC
CCCCCCCCCCCCCCCHHHHHHHHHHHHHEECCCCCCCEEEEEEECCCCCCCCCCCCCCCC
GG
CH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377; 10972810