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The map label for this gene is yflS

Identifier: 16077824

GI number: 16077824

Start: 829382

End: 830818

Strand: Direct

Name: yflS

Synonym: BSU07570

Alternate gene names: 16077824

Gene position: 829382-830818 (Clockwise)

Preceding gene: 16077823

Following gene: 16077825

Centisome position: 19.67

GC content: 46.0

Gene sequence:

>1437_bases
ATGGCTTCAGAAAAAGACGCAGGAAAACAGTCAGCAGTAAAGCTTGTTCCATTGCTTATTACTGTCGCTGTGGGACTAAT
CATCTGGTTTATTCCCGCTCCGTCCGGACTTGAACCTAAAGCTTGGCATTTGTTTGCGATTTTTGTCGCAACAATTATCG
GCTTTATCTCCAAGCCCTTGCCAATGGGTGCAATTGCAATTTTTGCATTGGCGGTTACTGCACTAACTGGAACACTATCA
ATTGAGGATACATTAAGCGGATTCGGGAATAAGACCATTTGGCTTATCGTTATCGCATTCTTTATTTCCCGGGGATTTAT
CAAAACCGGTCTCGGTGCGAGAATTTCGTATGTATTCGTTCAGAAATTCGGAAAAAAAACCCTTGGACTTTCTTATTCAC
TGCTATTCAGTGATTTAATACTTTCACCTGCTATTCCAAGTAATACGGCGCGTGCAGGAGGCATTATATTTCCTATTATC
AGATCATTATCCGAAACATTCGGATCAAGCCCGGCAAATGGAACAGAGAGAAAAATCGGTGCATTCTTATTAAAAACCGG
TTTTCAGGGGAATCTGATCACATCTGCTATGTTCCTGACAGCGATGGCGGCGAACCCGCTGATTGCCAAGCTGGCCCATG
ATGTCGCAGGGGTGGACTTAACATGGACAAGCTGGGCAATTGCCGCGATTGTACCGGGACTTGTAAGCTTAATCATCACG
CCGCTTGTGATTTACAAACTGTATCCGCCGGAAATCAAAGAAACACCGGATGCGGCGAAAATCGCAACAGAAAAACTGAA
AGAAATGGGACCGTTCAAAAAATCGGAGCTTTCCATGGTTATCGTGTTTCTTTTGGTGCTTGTGCTGTGGATTTTTGGCG
GCAGCTTCAACATCGACGCTACCACAACCGCATTGATCGGTTTGGCCGTTCTCTTATTATCACAAGTTCTGACTTGGGAT
GATATCAAGAAAGAACAGGGCGCTTGGGATACGCTCACTTGGTTTGCGGCGCTTGTCATGCTCGCCAACTTCTTGAATGA
ATTAGGCATGGTGTCTTGGTTCAGTAATGCCATGAAATCATCCGTATCAGGGTTCTCTTGGATTGTGGCATTCATCATTT
TAATTGTTGTGTATTATTACTCTCACTATTTCTTTGCAAGTGCGACAGCCCACATCAGTGCGATGTATTCAGCATTTTTG
GCTGTCGTCGTGGCAGCGGGCGCACCGCCGCTTTTAGCAGCGCTGAGCCTCGCGTTCATCAGCAACCTGTTCGGGTCAAC
GACTCACTACGGTTCTGGAGCGGCTCCGGTCTTCTTCGGAGCAGGCTACATCCCGCAAGGCAAATGGTGGTCCATCGGAT
TTATCCTGTCGATTGTTCATATCATCGTATGGCTTGTGATCGGCGGATTATGGTGGAAAGTACTAGGAATATGGTAG

Upstream 100 bases:

>100_bases
ACCTTTGTGTTTTTTAATTAATTAAAATGTTTATTAACTTAGTTAAGGAGTAGAATGGAAAAGGGGATCGGAAAACAAGT
ATATAGGAGGAGACCTATTT

Downstream 100 bases:

>100_bases
AAAGAAAAAGGCAGACGCGGTCTGCCTTTTTTTATTTTCACTCCTTCGTAAGAAAATGGATTTTGAAAAATGAGAAAATT
CCCTGTGAAAAATGGTATGA

Product: 2-oxoglutarate/malate transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MASEKDAGKQSAVKLVPLLITVAVGLIIWFIPAPSGLEPKAWHLFAIFVATIIGFISKPLPMGAIAIFALAVTALTGTLS
IEDTLSGFGNKTIWLIVIAFFISRGFIKTGLGARISYVFVQKFGKKTLGLSYSLLFSDLILSPAIPSNTARAGGIIFPII
RSLSETFGSSPANGTERKIGAFLLKTGFQGNLITSAMFLTAMAANPLIAKLAHDVAGVDLTWTSWAIAAIVPGLVSLIIT
PLVIYKLYPPEIKETPDAAKIATEKLKEMGPFKKSELSMVIVFLLVLVLWIFGGSFNIDATTTALIGLAVLLLSQVLTWD
DIKKEQGAWDTLTWFAALVMLANFLNELGMVSWFSNAMKSSVSGFSWIVAFIILIVVYYYSHYFFASATAHISAMYSAFL
AVVVAAGAPPLLAALSLAFISNLFGSTTHYGSGAAPVFFGAGYIPQGKWWSIGFILSIVHIIVWLVIGGLWWKVLGIW

Sequences:

>Translated_478_residues
MASEKDAGKQSAVKLVPLLITVAVGLIIWFIPAPSGLEPKAWHLFAIFVATIIGFISKPLPMGAIAIFALAVTALTGTLS
IEDTLSGFGNKTIWLIVIAFFISRGFIKTGLGARISYVFVQKFGKKTLGLSYSLLFSDLILSPAIPSNTARAGGIIFPII
RSLSETFGSSPANGTERKIGAFLLKTGFQGNLITSAMFLTAMAANPLIAKLAHDVAGVDLTWTSWAIAAIVPGLVSLIIT
PLVIYKLYPPEIKETPDAAKIATEKLKEMGPFKKSELSMVIVFLLVLVLWIFGGSFNIDATTTALIGLAVLLLSQVLTWD
DIKKEQGAWDTLTWFAALVMLANFLNELGMVSWFSNAMKSSVSGFSWIVAFIILIVVYYYSHYFFASATAHISAMYSAFL
AVVVAAGAPPLLAALSLAFISNLFGSTTHYGSGAAPVFFGAGYIPQGKWWSIGFILSIVHIIVWLVIGGLWWKVLGIW
>Mature_477_residues
ASEKDAGKQSAVKLVPLLITVAVGLIIWFIPAPSGLEPKAWHLFAIFVATIIGFISKPLPMGAIAIFALAVTALTGTLSI
EDTLSGFGNKTIWLIVIAFFISRGFIKTGLGARISYVFVQKFGKKTLGLSYSLLFSDLILSPAIPSNTARAGGIIFPIIR
SLSETFGSSPANGTERKIGAFLLKTGFQGNLITSAMFLTAMAANPLIAKLAHDVAGVDLTWTSWAIAAIVPGLVSLIITP
LVIYKLYPPEIKETPDAAKIATEKLKEMGPFKKSELSMVIVFLLVLVLWIFGGSFNIDATTTALIGLAVLLLSQVLTWDD
IKKEQGAWDTLTWFAALVMLANFLNELGMVSWFSNAMKSSVSGFSWIVAFIILIVVYYYSHYFFASATAHISAMYSAFLA
VVVAAGAPPLLAALSLAFISNLFGSTTHYGSGAAPVFFGAGYIPQGKWWSIGFILSIVHIIVWLVIGGLWWKVLGIW

Specific function: Might be a malate transporter

COG id: COG0471

COG function: function code P; Di- and tricarboxylate transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SLC13A transporter (TC 2.A.47) family

Homologues:

Organism=Escherichia coli, GI1786986, Length=468, Percent_Identity=39.957264957265, Blast_Score=346, Evalue=1e-96,
Organism=Escherichia coli, GI1789444, Length=476, Percent_Identity=36.1344537815126, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI1786829, Length=480, Percent_Identity=34.1666666666667, Blast_Score=295, Evalue=4e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YFLS_BACSU (O34726)

Other databases:

- EMBL:   D86417
- EMBL:   AL009126
- PIR:   F69811
- RefSeq:   NP_388638.1
- ProteinModelPortal:   O34726
- EnsemblBacteria:   EBBACT00000002384
- GeneID:   936122
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU07570
- NMPDR:   fig|224308.1.peg.757
- GenoList:   BSU07570
- GeneTree:   EBGT00050000001366
- HOGENOM:   HBG321139
- OMA:   ANTTAWE
- PhylomeDB:   O34726
- ProtClustDB:   CLSK886854
- BioCyc:   BSUB:BSU07570-MONOMER
- InterPro:   IPR001898
- TIGRFAMs:   TIGR00785

Pfam domain/function: PF00939 Na_sulph_symp

EC number: NA

Molecular weight: Translated: 51433; Mature: 51301

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xc11180c)-; HASH(0xcf537e4)-; HASH(0xdabddd4)-; HASH(0xd29d544)-; HASH(0xdaa9034)-; HASH(0xd8eab54)-; HASH(0xccb02a8)-; HASH(0xda9f2e8)-; HASH(0xd5fa6ac)-; HASH(0xda5c92c)-; HASH(0xd12dd3c)-; HASH(0xd793fd4)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASEKDAGKQSAVKLVPLLITVAVGLIIWFIPAPSGLEPKAWHLFAIFVATIIGFISKPL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
PMGAIAIFALAVTALTGTLSIEDTLSGFGNKTIWLIVIAFFISRGFIKTGLGARISYVFV
CHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHCCHHHHCCCHHHHHHHH
QKFGKKTLGLSYSLLFSDLILSPAIPSNTARAGGIIFPIIRSLSETFGSSPANGTERKIG
HHHCCHHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHH
AFLLKTGFQGNLITSAMFLTAMAANPLIAKLAHDVAGVDLTWTSWAIAAIVPGLVSLIIT
HHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHH
PLVIYKLYPPEIKETPDAAKIATEKLKEMGPFKKSELSMVIVFLLVLVLWIFGGSFNIDA
HHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCH
TTTALIGLAVLLLSQVLTWDDIKKEQGAWDTLTWFAALVMLANFLNELGMVSWFSNAMKS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVSGFSWIVAFIILIVVYYYSHYFFASATAHISAMYSAFLAVVVAAGAPPLLAALSLAFI
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
SNLFGSTTHYGSGAAPVFFGAGYIPQGKWWSIGFILSIVHIIVWLVIGGLWWKVLGIW
HHHHCCCCCCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure 
ASEKDAGKQSAVKLVPLLITVAVGLIIWFIPAPSGLEPKAWHLFAIFVATIIGFISKPL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
PMGAIAIFALAVTALTGTLSIEDTLSGFGNKTIWLIVIAFFISRGFIKTGLGARISYVFV
CHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHCCHHHHCCCHHHHHHHH
QKFGKKTLGLSYSLLFSDLILSPAIPSNTARAGGIIFPIIRSLSETFGSSPANGTERKIG
HHHCCHHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHH
AFLLKTGFQGNLITSAMFLTAMAANPLIAKLAHDVAGVDLTWTSWAIAAIVPGLVSLIIT
HHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHH
PLVIYKLYPPEIKETPDAAKIATEKLKEMGPFKKSELSMVIVFLLVLVLWIFGGSFNIDA
HHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCH
TTTALIGLAVLLLSQVLTWDDIKKEQGAWDTLTWFAALVMLANFLNELGMVSWFSNAMKS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVSGFSWIVAFIILIVVYYYSHYFFASATAHISAMYSAFLAVVVAAGAPPLLAALSLAFI
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
SNLFGSTTHYGSGAAPVFFGAGYIPQGKWWSIGFILSIVHIIVWLVIGGLWWKVLGIW
HHHHCCCCCCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377; 12949159