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The map label for this gene is yclM

Identifier: 16077447

GI number: 16077447

Start: 430623

End: 431987

Strand: Reverse

Name: yclM

Synonym: BSU03790

Alternate gene names: 16077447

Gene position: 431987-430623 (Counterclockwise)

Preceding gene: 16077452

Following gene: 255767119

Centisome position: 10.25

GC content: 49.08

Gene sequence:

>1365_bases
ATGAAGGTCGTTAAATTCGGAGGCAGCTCACTTGCTTCAGGCGCCCAGCTTGACAAGGTGTTTCACATCGTTACCTCAGA
TCCGGCACGGAAAGCTGTAGTCGTTTCAGCTCCGGGAAAACACTATGCCGAGGATACGAAAGTGACTGATCTCTTAATCG
CATGTGCAGAACAATATTTGGCAACAGGCAGCGCACCTGAACTGGCGGAAGCTGTTGTGGAACGGTACGCTCTCATCGCC
AATGAGCTTCAGCTGGGGCAAAGCATTATCGAAAAAATCAGAGATGATCTGTTTACGCTTTTAGAAGGAGACAAAAGCAA
TCCCGAACAATACCTTGACGCAGTCAAGGCCAGCGGAGAGGATAACAATGCCAAACTGATCGCCGCTTACTTCCGTTATA
AAGGCGTCAAAGCGGAATATGTAAACCCGAAGGATGCCGGCCTCTTTGTGACAAATGAACCCGGCAACGCGCAAGTTCTT
CCTGAATCCTATCAAAACCTCTATCGTCTTCGGGAACGTGACGGACTCATCATTTTTCCCGGTTTTTTCGGATTCAGCAA
GGATGGCGATGTGATCACATTCTCACGGAGCGGTTCTGATATTACCGGTTCGATTCTTGCCAACGGACTACAAGCCGATT
TGTACGAAAACTTTACAGACGTAGACGCTGTGTATTCTGTCAATCCGTCCTTCGTTGAGAATCCAAAGGAAATCAGCGAG
CTGACATATAGAGAGATGCGGGAGCTGTCCTACGCGGGTTTTTCAGTGTTTCATGATGAAGCGCTCATTCCGGCATTCAG
AGCGGGGATTCCTGTTCAGATCAAAAATACGAACAACCCCTCAGCCGAAGGCACCCGCGTCGTCAGCAAGCGGGATAACA
CAAACGGGCCTGTCGTCGGCATTGCCAGCGACACCGGTTTTTGCAGCATTTATATCAGCAAGTATCTCATGAACAGAGAA
ATCGGTTTTGGCCGCAGAGCCCTTCAAATCCTGGAGGAGCATGGTTTGACGTATGAGCATGTTCCATCGGGAATCGATGA
CATGACAATCATTTTACGGCAGGGGCAAATGGATGCCGCCACTGAACGCAGCGTCATCAAACGGATCGAAGAGGATTTGC
ATGCCGATGAAGTCATCGTCGAGCATCATCTCGCCCTGATTATGGTTGTAGGGGAAGCGATGCGCCACAATGTCGGTACA
ACGGCAAGAGCCGCCAAAGCATTATCAGAGGCACAGGTGAATATCGAAATGATCAATCAGGGGTCTTCTGAAGTGAGCAT
GATGTTTGGCGTAAAAGAAGCCGAAGAAAGAAAAGCCGTTCAAGCGTTATACCAGGAATTTTTCGCGGGCGTGCTGATCT
CTTAA

Upstream 100 bases:

>100_bases
GTTCTCCTGAGAAAGAAATTCCCCCTTGATTTACAGTACTGAAAGAGAGATAATAAATACTAAATTTGTGTGAAAATTTT
AAACATTAGGAGATGTAAAC

Downstream 100 bases:

>100_bases
TCGTACATAAATAGCGGGCGGCAGCGCCCGCTATTTTTTTATATCACCTTCATATAGCCGAATTCTTCATTTTTTCTCTT
GTCCACTATTATCCCTGCCA

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MKVVKFGGSSLASGAQLDKVFHIVTSDPARKAVVVSAPGKHYAEDTKVTDLLIACAEQYLATGSAPELAEAVVERYALIA
NELQLGQSIIEKIRDDLFTLLEGDKSNPEQYLDAVKASGEDNNAKLIAAYFRYKGVKAEYVNPKDAGLFVTNEPGNAQVL
PESYQNLYRLRERDGLIIFPGFFGFSKDGDVITFSRSGSDITGSILANGLQADLYENFTDVDAVYSVNPSFVENPKEISE
LTYREMRELSYAGFSVFHDEALIPAFRAGIPVQIKNTNNPSAEGTRVVSKRDNTNGPVVGIASDTGFCSIYISKYLMNRE
IGFGRRALQILEEHGLTYEHVPSGIDDMTIILRQGQMDAATERSVIKRIEEDLHADEVIVEHHLALIMVVGEAMRHNVGT
TARAAKALSEAQVNIEMINQGSSEVSMMFGVKEAEERKAVQALYQEFFAGVLIS

Sequences:

>Translated_454_residues
MKVVKFGGSSLASGAQLDKVFHIVTSDPARKAVVVSAPGKHYAEDTKVTDLLIACAEQYLATGSAPELAEAVVERYALIA
NELQLGQSIIEKIRDDLFTLLEGDKSNPEQYLDAVKASGEDNNAKLIAAYFRYKGVKAEYVNPKDAGLFVTNEPGNAQVL
PESYQNLYRLRERDGLIIFPGFFGFSKDGDVITFSRSGSDITGSILANGLQADLYENFTDVDAVYSVNPSFVENPKEISE
LTYREMRELSYAGFSVFHDEALIPAFRAGIPVQIKNTNNPSAEGTRVVSKRDNTNGPVVGIASDTGFCSIYISKYLMNRE
IGFGRRALQILEEHGLTYEHVPSGIDDMTIILRQGQMDAATERSVIKRIEEDLHADEVIVEHHLALIMVVGEAMRHNVGT
TARAAKALSEAQVNIEMINQGSSEVSMMFGVKEAEERKAVQALYQEFFAGVLIS
>Mature_454_residues
MKVVKFGGSSLASGAQLDKVFHIVTSDPARKAVVVSAPGKHYAEDTKVTDLLIACAEQYLATGSAPELAEAVVERYALIA
NELQLGQSIIEKIRDDLFTLLEGDKSNPEQYLDAVKASGEDNNAKLIAAYFRYKGVKAEYVNPKDAGLFVTNEPGNAQVL
PESYQNLYRLRERDGLIIFPGFFGFSKDGDVITFSRSGSDITGSILANGLQADLYENFTDVDAVYSVNPSFVENPKEISE
LTYREMRELSYAGFSVFHDEALIPAFRAGIPVQIKNTNNPSAEGTRVVSKRDNTNGPVVGIASDTGFCSIYISKYLMNRE
IGFGRRALQILEEHGLTYEHVPSGIDDMTIILRQGQMDAATERSVIKRIEEDLHADEVIVEHHLALIMVVGEAMRHNVGT
TARAAKALSEAQVNIEMINQGSSEVSMMFGVKEAEERKAVQALYQEFFAGVLIS

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains

Homologues:

Organism=Escherichia coli, GI1786183, Length=470, Percent_Identity=28.2978723404255, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1790455, Length=458, Percent_Identity=26.6375545851528, Blast_Score=112, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6320893, Length=496, Percent_Identity=23.7903225806452, Blast_Score=116, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AK3_BACSU (P94417)

Other databases:

- EMBL:   D50453
- EMBL:   AL009126
- PIR:   A69763
- RefSeq:   NP_388261.1
- ProteinModelPortal:   P94417
- SMR:   P94417
- EnsemblBacteria:   EBBACT00000000135
- GeneID:   938276
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU03790
- NMPDR:   fig|224308.1.peg.380
- GenoList:   BSU03790
- GeneTree:   EBGT00050000001782
- HOGENOM:   HBG724395
- OMA:   EIGFGRR
- PhylomeDB:   P94417
- ProtClustDB:   PRK09034
- BioCyc:   BSUB:BSU03790-MONOMER
- BioCyc:   MetaCyc:MONOMER-6563
- BRENDA:   2.7.2.4
- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR001341
- InterPro:   IPR018042
- Gene3D:   G3DSA:3.40.1160.10
- TIGRFAMs:   TIGR00657

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT; SSF53633 Aa_kinase

EC number: =2.7.2.4

Molecular weight: Translated: 49826; Mature: 49826

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVKFGGSSLASGAQLDKVFHIVTSDPARKAVVVSAPGKHYAEDTKVTDLLIACAEQYL
CEEEEECCCCCCCCHHHHHEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHH
ATGSAPELAEAVVERYALIANELQLGQSIIEKIRDDLFTLLEGDKSNPEQYLDAVKASGE
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
DNNAKLIAAYFRYKGVKAEYVNPKDAGLFVTNEPGNAQVLPESYQNLYRLRERDGLIIFP
CCCCEEEEEEHHHCCCEEEECCCCCCEEEEECCCCCCEECHHHHHHHHHHHHCCCEEEEC
GFFGFSKDGDVITFSRSGSDITGSILANGLQADLYENFTDVDAVYSVNPSFVENPKEISE
CCCCCCCCCCEEEEECCCCCCCHHHHHCCHHHHHHCCCCCCCCEEECCCHHHCCHHHHHH
LTYREMRELSYAGFSVFHDEALIPAFRAGIPVQIKNTNNPSAEGTRVVSKRDNTNGPVVG
HHHHHHHHHHHCCHHHHCCCHHHHHHHCCCCEEEECCCCCCCCCCEEEECCCCCCCCEEE
IASDTGFCSIYISKYLMNREIGFGRRALQILEEHGLTYEHVPSGIDDMTIILRQGQMDAA
EECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHCCCCCCCEEEEEECCCCCHH
TERSVIKRIEEDLHADEVIVEHHLALIMVVGEAMRHNVGTTARAAKALSEAQVNIEMINQ
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEEECC
GSSEVSMMFGVKEAEERKAVQALYQEFFAGVLIS
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVVKFGGSSLASGAQLDKVFHIVTSDPARKAVVVSAPGKHYAEDTKVTDLLIACAEQYL
CEEEEECCCCCCCCHHHHHEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHH
ATGSAPELAEAVVERYALIANELQLGQSIIEKIRDDLFTLLEGDKSNPEQYLDAVKASGE
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
DNNAKLIAAYFRYKGVKAEYVNPKDAGLFVTNEPGNAQVLPESYQNLYRLRERDGLIIFP
CCCCEEEEEEHHHCCCEEEECCCCCCEEEEECCCCCCEECHHHHHHHHHHHHCCCEEEEC
GFFGFSKDGDVITFSRSGSDITGSILANGLQADLYENFTDVDAVYSVNPSFVENPKEISE
CCCCCCCCCCEEEEECCCCCCCHHHHHCCHHHHHHCCCCCCCCEEECCCHHHCCHHHHHH
LTYREMRELSYAGFSVFHDEALIPAFRAGIPVQIKNTNNPSAEGTRVVSKRDNTNGPVVG
HHHHHHHHHHHCCHHHHCCCHHHHHHHCCCCEEEECCCCCCCCCCEEEECCCCCCCCEEE
IASDTGFCSIYISKYLMNREIGFGRRALQILEEHGLTYEHVPSGIDDMTIILRQGQMDAA
EECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHCCCCCCCEEEEEECCCCCHH
TERSVIKRIEEDLHADEVIVEHHLALIMVVGEAMRHNVGTTARAAKALSEAQVNIEMINQ
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEEECC
GSSEVSMMFGVKEAEERKAVQALYQEFFAGVLIS
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377